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    • 8. 发明申请
    • METHOD OF SPERM SORTING USING LIPOSOMES AND/OR AN INHIBITOR OF SLO3 POTASSIUM CHANNEL
    • WO2023012247A1
    • 2023-02-09
    • PCT/EP2022/071882
    • 2022-08-03
    • UNIVERS 2020
    • CARION, OlivierSCHMITT, Eric
    • C12N5/076A01N1/02A61D19/02A61K31/47
    • The invention relates to a method of sperm sorting of the spermatozoa contained in the sperm of a mammalian species, said method comprising a step of mixing the sperm sample with liposomes and/or at least one inhibitor of Slo3 potassium channel. In particular, the invention also relates to a method of sperm sorting of the spermatozoa contained in the sperm of a mammalian species, said method comprising the following steps: - mixing said sperm and a fluorescent dye for labelling sperm DNA with a sample fluid into a fluid mixture; - providing a flow cytometric sperm sorting device selected from the in-air droplet systems and the flow cell tip close systems; - using a sperm sorting device on said labelled fluid mixture employing a high energy emitting light source regarding the specific excitation spectrum of the dye, thereby providing each spermatozoon encased in a single droplet of fluid which is hydrodynamically oriented by the use of sheath fluid, identifying the DNA content of said droplet of fluid, assigning an electric charge corresponding to the chromosome status of said droplet and sorting said droplet; and directing said droplet by electrostatic deflection into one of separate collected tubes each containing a catch fluid, wherein the sample fluid and/or the catch fluid comprises liposomes and/or at least one inhibitor of Slo3 potassium channel.