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1. 发明申请

WO2016015018A1 NANOPIPETTE ANALYSIS OF POLYMERS 审中-公开
标题翻译：聚合物纳米材料分析
公开(公告)号：WO2016015018A1
公开(公告)日：2016-01-28
申请号：PCT/US2015/042123
申请日：2015-07-24
申请人： MIR, Kalim
发明人： MIR, Kalim
IPC分类号： G01Q60/44 , C12Q1/02 , C12Q1/68 , B01L3/02 , B82Y5/00
CPC分类号： C12Q1/6825 , B01L3/021 , B01L2200/0663 , B01L2200/143 , B01L2300/0896 , B01L2400/0415 , B01L2400/0421 , B01L2400/043 , B01L2400/0487 , B82Y5/00 , G01N33/48721 , G01N33/5438 , G01Q60/44 , C12Q2563/116 , C12Q2565/631
摘要： The disclosure relates to devices and instruments for detecting and individually analyzing biomolecules, biomolecular complexes and biomolecules with ligands attached thereon.
摘要翻译：本公开涉及用于检测和分别分析附着于其上的配体的生物分子，生物分子复合物和生物分子的装置和仪器。

2. 发明申请

WO2013012452A3 NANOPIPETTE APPARATUS FOR MANIPULATING CELLS 审中-公开
标题翻译：用于操作细胞的纳米技术装置
公开(公告)号：WO2013012452A3
公开(公告)日：2013-04-25
申请号：PCT/US2012026781
申请日：2012-02-27
申请人： UNIV CALIFORNIA , SEGER R ADAM , ACTIS PAOLO , VILOZNY BOAZ , POURMAND NADER
发明人： SEGER R ADAM , ACTIS PAOLO , VILOZNY BOAZ , POURMAND NADER
IPC分类号： C12M1/42 , C12N13/00
CPC分类号： B82Y5/00 , B01J2219/00371 , B01L3/502715 , B01L3/50273 , B01L2300/0645 , B82Y15/00 , G01N33/48728 , G01Q60/44
摘要： Disclosed herein are methods and systems for controlled ejection of desired material onto surfaces including single cells using nanopipettes, as well as ejection and into cells. Some systems are directed to a method and system comprising nanopipettes combined with an xyz controller for depositing a user defined pattern on an arbitrary substrate for the purpose of controlled cell adhesion and growth. Alternate embodiments are directed to a method and system comprising nanopipettes combined with an xyz controller and electronic control of a voltage differential in a bore of the nanopipette electroosmotically injecting material into a cell in a high-throughput manner and with minimal damage to the cell. Yet other embodiments are directed to method and system comprising functionalized nanopipettes combined with scanning ion conductance microscopy for studying molecular interactions and detection of biomolecules inside a single living cell.
摘要翻译：本文公开了用于将所需材料控制喷射到表面上的方法和系统，包括使用纳米移液管的单个细胞，以及喷射和进入细胞。一些系统涉及一种方法和系统，其包括与xyz控制器组合的纳米移液器，用于将用户定义的图案沉积在任意基底上，以便控制细胞粘附和生长。替代实施例涉及一种方法和系统，其包括与xyz控制器组合的纳米移液器，并且以高通量方式以高通量方式电动地将材料的孔中的电压差电子控制到电池中并以最小的细胞损伤来电子控制。其他实施方案涉及包括与扫描离子电导显微镜结合的功能化纳米小片剂用于研究单个活细胞内的分子相互作用和生物分子的检测的方法和系统。

3. 发明申请

WO2012122029A2 NANOPORE DEVICE FOR REVERSIBLE ION AND MOLECULE SENSING OR MIGRATION 审中-公开
标题翻译：用于可逆离子和分子检测或迁移的NANOPORE装置
公开(公告)号：WO2012122029A2
公开(公告)日：2012-09-13
申请号：PCT/US2012/027531
申请日：2012-03-02
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , POURMAND, Nader , VILOZNY, Boaz , ACTIS, Paolo , SEGER, R., Adam , SINGARAM, Bakthan
发明人： POURMAND, Nader , VILOZNY, Boaz , ACTIS, Paolo , SEGER, R., Adam , SINGARAM, Bakthan
IPC分类号： G01N27/26 , G01N27/49 , G01N35/00
CPC分类号： G01N27/44791 , B01L3/021 , B01L3/50273 , B01L2300/0896 , B82Y35/00 , G01N33/48721 , G01Q60/44
摘要： Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.
摘要翻译：公开了利用适用于电化学传感电路的纳米管来检测离子迁移和结合的方法和装置。纳米管可以在其内孔上用金属螯合剂功能化，用于结合和感测金属离子或其他特定结合分子如硼酸以结合和检测葡萄糖。这种功能化的纳米管被包含在电传感器中，该传感器检测纳米吸管何时选择性地和可逆地结合离子或小分子。还公开了一种包含纳米管的纳米反应器，用于通过电压引导的离子迁移来控制在水溶液中的沉淀，其中离子可以通过孔中的排斥电荷被引导离开内孔。

4. 发明申请

WO2014160036A1 NANOPIPETTE DEVICE AND METHOD FOR SUBCELLULAR ANALYSIS 审中-公开
公开(公告)号：WO2014160036A1
公开(公告)日：2014-10-02
申请号：PCT/US2014/025682
申请日：2014-03-13
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人： ACTIS, Paolo , MAALOUF, Michelle, M. , POURMAND, Nader
IPC分类号： G01N33/53 , C12M1/34 , C12N5/00
CPC分类号： C12N15/1003 , C12M47/06 , C12Q1/6806 , C12Q1/686 , C12Q1/6874 , C12Q1/6876 , C12Q2600/158 , G01N33/573 , G01N2035/1034 , G01N2333/91 , G01N2440/14 , G01Q60/44
摘要： Described herein are devices and methods for extracting cellular material from living cells and then depositing them into a receptacle in a nanoliter scale. Using a nanopipette integrated into a scanning ion conductance microscope (SICM), extraction of mitochondrial DNA from human BJ fibroblasts and Green Fluorescent Protein (GFP) transcripts from HeLa/GFP cells was achieved with minimal disruption to the cellular milieu and without chemical treatment prior to obtaining the isolated sample. Success of the extraction was confirmed by fluorescence microscopy and PCR analysis of the extracted material. The method and apparatus may be applied to many different cell types and intracellular targets, allowing not only single cell analysis, but single subcellular compartment analysis of materials extracted in their native state.
摘要翻译：本文描述了用于从活细胞中提取细胞材料然后将其以纳升级规放置在容器中的装置和方法。使用集成到扫描离子电导显微镜（SICM）中的纳米针管，从HeLa / GFP细胞的人BJ成纤维细胞和绿色荧光蛋白（GFP）转录物中提取线粒体DNA获得了细胞环境的最小破坏，而在没有化学处理之前获得分离的样品。通过提取的材料的荧光显微镜和PCR分析证实提取的成功。该方法和装置可以应用于许多不同的细胞类型和细胞内靶标，不仅允许单细胞分析，而且允许在其天然状态下提取的材料进行单次细胞室分析。

5. 发明申请

WO2012122029A3 NANOPORE DEVICE FOR REVERSIBLE ION AND MOLECULE SENSING OR MIGRATION 审中-公开
标题翻译：用于可逆离子和分子感测或移动的纳米设备
公开(公告)号：WO2012122029A3
公开(公告)日：2012-11-22
申请号：PCT/US2012027531
申请日：2012-03-02
申请人： UNIV CALIFORNIA , POURMAND NADER , VILOZNY BOAZ , ACTIS PAOLO , SEGER R ADAM , SINGARAM BAKTHAN
发明人： POURMAND NADER , VILOZNY BOAZ , ACTIS PAOLO , SEGER R ADAM , SINGARAM BAKTHAN
IPC分类号： G01N27/26 , G01N27/49 , G01N35/00
CPC分类号： G01N27/44791 , B01L3/021 , B01L3/50273 , B01L2300/0896 , B82Y35/00 , G01N33/48721 , G01Q60/44
摘要： Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.
摘要翻译：公开了用于检测离子迁移和结合的方法和装置，利用适用于电化学感测电路的纳米管。纳米胶管可以在其内孔上用金属螯合剂进行官能化，用于结合和感测金属离子或其它特异性结合分子例如硼酸用于结合和感测葡萄糖。这种功能化的纳米移液管包含在电传感器中，该传感器检测纳米管片何时选择性地和可逆地结合离子或小分子。还公开了一种纳米反应器，其包括用于通过电压导向离子迁移控制水溶液中的沉淀的纳米滴管，其中离子可以通过孔中的排斥电荷引出到内孔内。

6. 发明申请

WO2008075842A1 SCANNING ION CONDUCTANCE MICROSCOPY HAVING MULTIPIPETTE 审中-公开
标题翻译：扫描离子导管显微镜
公开(公告)号：WO2008075842A1
公开(公告)日：2008-06-26
申请号：PCT/KR2007/006244
申请日：2007-12-04
申请人： KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE , AHN, Sang-jung , KIM, Dal-hyun , PARK, Byong-chon , KOO, Ja-yong , MOON, Dae-won
发明人： AHN, Sang-jung , KIM, Dal-hyun , PARK, Byong-chon , KOO, Ja-yong , MOON, Dae-won
IPC分类号： G01N27/26
CPC分类号： G01Q60/44 , G01Q70/06
摘要： A scanning ion conductance microscope having a multipipette is provided, which includes multiple pipettes to measure ion currents so that it is less affected by noise, thereby achieving a more accurate image. The multipipette measures changes in the levels of ion currents flowing through pipette holes of the multiple pipettes, each having a pipette electrode, and then images the measurement. A sample is measured by comparing ion currents flowing through the pipette holes of the multipipette.
摘要翻译：提供了具有多接头的扫描离子电导显微镜，其包括多个移液管以测量离子电流，使其受到噪声的影响较小，从而获得更准确的图像。多面吸取器测量流过多个移液管的移液管孔的离子流量的变化，每个移液管均带有移液管电极，然后对测量进行成像。通过比较流过多吸嘴的移液管孔的离子流来测量样品。

7. 发明申请

WO2002077627A2 PATCH-CLAMPING AND ITS USE IN ANALYSING SUBCELLULAR FEATURES 审中-公开
标题翻译：掌握夹具及其在分析细胞特征中的应用
公开(公告)号：WO2002077627A2
公开(公告)日：2002-10-03
申请号：PCT/GB2002/001382
申请日：2002-03-22
申请人： IMPERIAL COLLEGE INNOVATIONS LIMITED , KORCHEV, Yuri, Evgenievich , LAB, Max, Joseph
发明人： KORCHEV, Yuri, Evgenievich , LAB, Max, Joseph
IPC分类号： G01N27/00
CPC分类号： G01Q60/44 , G01N33/48728
摘要： A method investigating a cell, comprises bringing a probe close to the surface of the cell or a part thereof, at a controlled distance therefrom; and into contact with the surface, essentially normal to the surface, to achieve patch-clamping.
摘要翻译：一种调查细胞的方法，包括使探头靠近细胞表面或其一部分; 并与表面接触，基本上垂直于表面，以实现贴片夹紧。

8. 发明申请

WO2017064354A1 LINKED MICROMECHANICAL POSITIONING APPARATUS FOR REAL-TIME TESTING AND MEASUREMENT 审中-公开
标题翻译：用于实时测试和测量的链接微机械定位设备
公开(公告)号：WO2017064354A1
公开(公告)日：2017-04-20
申请号：PCT/FI2015/050689
申请日：2015-10-13
申请人： SENSAPEX OY
发明人： VÄHÄSÖYRINKI, Mikko , VIMPARI, Markku
IPC分类号： G01Q20/00 , B82Y35/00 , B25J7/00 , G02B21/32 , B81B3/00
CPC分类号： B25J7/00 , B25J13/02 , B81B3/00 , G01N33/48707 , G01Q10/06 , G01Q30/14 , G01Q60/44 , G01Q70/06 , G02B21/32
摘要： The invention relates to a positioning device (120, 154), for example for testing, comprising a micromechanical positioning actuator (130) for causing movement of a probe (150, 151) with respect to a target (110), a positioning controller (145), the positioning controller (145) having an output coupled to the actuator (130) for controlling the movement, and the positioning controller (145) having a steering input (156) for receiving a steering signal to the positioning controller (145), and the positioning controller (145) arranged to control the movement based on the steering signal. The measurement device may have memory for storing positioning control instructions (300). The positioning controller (145) may be arranged to control said movement based on said steering signal and said positioning control instructions (300).
摘要翻译：本发明涉及一种例如用于测试的定位装置（120,154），其包括微机械定位致动器（130），用于使探针（150,151）相对于目标所述定位控制器（145）具有耦合到所述致动器（130）以用于控制所述移动的输出，并且所述定位控制器（145）具有用于接收转向的转向输入（156）信号提供给定位控制器（145），并且定位控制器（145）设置成基于转向信号来控制运动。测量装置可具有用于存储定位控制指令的存储器（300）。定位控制器（145）可以被布置为基于所述转向信号和所述定位控制指令（300）来控制所述移动。

9. 发明申请

WO2015136260A1 SCANNING ION CONDUCTANCE MICROSCOPY 审中-公开
标题翻译：扫描离子导管显微镜
公开(公告)号：WO2015136260A1
公开(公告)日：2015-09-17
申请号：PCT/GB2015/050688
申请日：2015-03-10
申请人： IONSCOPE LTD
发明人： RICHARDSON, Andrew James
IPC分类号： G01Q10/06 , G01Q60/44
CPC分类号： G01Q60/44 , G01Q10/02 , G01Q10/06 , G01Q60/02 , G01Q60/60
摘要： A method for interrogating a surface of a sample bathed in electrolyte solution using SICM, comprising: controlling the potential between first and second electrodes bathed in the electrolyte solution to induce an ion current in the electrolyte solution, a submerged portion of the first electrode being contained within a micropipette and the second electrode being external to the micropipette; recording the ion current whilst controlling the micropipette to move with respect to a stage supporting the sample; and determining, from the ion current and calibration data, the surface height profile of the sample. Said potential can be controlled according to a spread spectrum modulated signal. Said micropipette motion can be according to an AC mode pattern having a modulation frequency greater than a resonant frequency of an assembly of the micropipette, first electrode and a first piezoelectric actuator configured to control z-axis motion of said micropipette.
摘要翻译：一种用于询问使用SICM浸在电解质溶液中的样品的表面的方法，包括：控制浸在电解质溶液中的第一和第二电极之间的电位，以引起电解质溶液中的离子电流，第一电极的浸没部分被包含并且所述第二电极位于所述微量移液管外部; 记录离子电流同时控制微量移液器相对于支撑样品的载物台移动; 以及从离子电流和校准数据确定样品的表面高度分布。可以根据扩频调制信号来控制所述电位。所述微量移液管运动可以根据具有大于微量移液管组件，第一电极和被配置为控制所述微量移液管的z轴运动的第一压电致动器的组件的共振频率的调制频率的AC模式图案。

10. 发明申请

WO2009095720A3 SCANNING ION CONDUCTANCE MICROSCOPY 审中-公开
标题翻译：扫描离子导管显微镜
公开(公告)号：WO2009095720A3
公开(公告)日：2009-10-29
申请号：PCT/GB2009050092
申请日：2009-02-02
申请人： NOVAK PAVEL , LI CHAO , SHEVCHUK ANDREW , OSTANIN VICTOR , KLENERMAN DAVID , KORCHEV YURI , FROLENKOV GREGORY , CLARKE RICHARD , IMP INNOVATIONS LTD
发明人： NOVAK PAVEL , LI CHAO , SHEVCHUK ANDREW , OSTANIN VICTOR , KLENERMAN DAVID , KORCHEV YURI , FROLENKOV GREGORY , CLARKE RICHARD
IPC分类号： G01Q10/06 , G01Q60/44
CPC分类号： G01Q60/44 , B82Y35/00 , G01Q10/00 , G01Q10/02 , G01Q10/065
摘要： A method for interrogating a surface using scanning ion conductance microscopy (SICM), comprising the steps of: a) repeatedly bringing a SICM probe into proximity with the surface at discrete, spaced locations in a region of the surface and measuring surface height at each location; b) estimating surface roughness or other characteristic for the region based upon the surface height measurements; and c) repeatedly bringing the probe into proximity with the surface at discrete, spaced locations in the region, the number and location of which is based upon the estimated surface roughness or other characteristic in the region, and obtaining an image of the region with a resolution adapted to the surface roughness or other characteristic.
摘要翻译：一种使用扫描离子电导显微镜（SICM）询问表面的方法，包括以下步骤：a）在表面的一个区域中的离散的间隔位置处重复地使SICM探针接近表面，并在每个位置测量表面高度 ; b）基于表面高度测量来估计区域的表面粗糙度或其他特性; 以及c）在所述区域中的离散的间隔位置处重复地使所述探针靠近所述表面，所述位置的数量和位置基于所估计的表面粗糙度或所述区域中的其它特征，并且获得所述区域的图像分辨率适应于表面粗糙度或其他特性。

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