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1. 发明申请

WO2022243437A1 SAMPLE PREPARATION WITH OPPOSITELY ORIENTED GUIDE POLYNUCLEOTIDES 审中-公开
公开(公告)号：WO2022243437A1
公开(公告)日：2022-11-24
申请号：PCT/EP2022/063585
申请日：2022-05-19
申请人： KWS SAAT SE & CO. KGAA , KEYGENE N.V.
发明人： JANTO, Benjamin
IPC分类号： C12Q1/6806
摘要： The invention relates to a method for preparing a sample (400) comprising sample polynucleotides (602) for sequencing, wherein at least one of the sample polynucleotides comprises a known sequence (605), the method comprising: protecting (102) the ends of the sample polynucleotides, contacting (104) at least first and second nucleoprotein particles (608, 702, 704) with the protected sample polynucleotides, wherein each first nucleoprotein particle comprises an effector protein and a first guide polynucleotide (706) and each second nucleoprotein particle comprises an effector protein and a second guide polynucleotide (708), wherein the sequences of the first and second guide polynucleotides are different and are selected such that o the first guide polynucleotides cause the effector proteins to cut the known sequence at a first cleavage site (722); o the second guide polynucleotides cause the effector proteins to cut the known sequence at a second cleavage site (724); o wherein the first and second cleavage sites define an in-between sequence (614) as the part of the known sequence between the first and second cleavage sites (722, 724); o whereby the first and second guide polynucleotides respectively comprise a binding sequence (715, 713) whose position and orientation within the known sequence is selected such that when the sample is contacted with a sequencing adapter (616), the sequencing adapter selectively binds to the ends of the sample polynucleotide fragments created by the cutting which do not comprise the in- between sequence; adding (106) sequencing adapters to the sample.

2. 发明申请

WO2020089448A1 DUAL GUIDE RNA FOR CRISPR/CAS GENOME EDITING IN PLANTS CELLS 审中-公开
公开(公告)号：WO2020089448A1
公开(公告)日：2020-05-07
申请号：PCT/EP2019/079950
申请日：2019-11-01
申请人： KEYGENE N.V.
发明人： LHUISSIER, Franck Georges Paul
IPC分类号： C12N15/82
摘要： The invention pertains to a method for targeted modification of DNA in a plant cell, comprising a step of contacting the DNA with an RNA-guided CRISPR-system nuclease complex, wherein the complex comprises a crRNA anda tracrRNA as separate molecules. The invention further pertains to said RNA-guided CRISPR-system nuclease complex for targeting of DNA in a plant cell and kits comprising the RNA-guided CRISPR-system nuclease complex or constructs encoding the same.

3. 发明申请

WO2019193143A1 IMPROVED SHOOT REGENERATION BY OVEREXPRESSION OF CHK GENES 审中-公开
公开(公告)号：WO2019193143A1
公开(公告)日：2019-10-10
申请号：PCT/EP2019/058614
申请日：2019-04-05
申请人： KEYGENE N.V.
发明人： DE BOTH, Michiel Theodoor Jan , OP DEN CAMP, Rik Hubertus Martinus , KLOOSTERMAN, Bjorn Alexander , FIERENS-ONSTENK, Bernarda Gerharda Johanna
IPC分类号： C12N15/82
摘要： Genetic modification of plants is hampered by the limited capacity of plant cells to regenerate. The current invention solves this problem by introducing or increasing the expression of a histidine kinase in a plant cell. Preferred histidine kinases are at least one of CHK2, CHK3 and CHK4.The invention therefore concerns a method for improving a cytokinin-induced regeneration capacity of a plant cell, wherein the method comprises a step of increasing or introducing the expression of a histidine kinase in the plant cell.The invention further pertains to a method for regenerating a plant, wherein the method comprises a step of introducing or increasing the expression of a histidine kinase and to a plant obtainable from such method. Moreover, the method concerns the use of at least one of CHK2, CHK3 and CHK4 for improving a cytokinin-induced regeneration capacity of a plant.

4. 发明申请

WO2018149915A1 METHODS OF TARGETED GENETIC ALTERATION IN PLANT CELLS 审中-公开
公开(公告)号：WO2018149915A1
公开(公告)日：2018-08-23
申请号：PCT/EP2018/053775
申请日：2018-02-15
申请人： KEYGENE N.V.
发明人： BUNDOCK, Paul
IPC分类号： A01H1/06 , C12Q1/68 , C12N15/11 , C12N15/90 , C12N15/82
摘要： The current invention relates to methods of targeted genetic alteration in cells, preferably plant cells, as well as to plant cells and plants thus obtained using at least a fusion protein comprising a site-specific nuclease domain and a deaminase domain, or a construct encoding the same. The method also provides for a composition and a kit comprising a combination of a first fusion protein comprising a cytosine deaminase domain and a second fusion protein comprising an adenine deaminase domain, preferably for use in the method of the invention. The method provides for targeted alteration of a DNA duplex in plant cells with increased efficacy.

5. 发明申请

WO2018115389A1 METHODS OF TARGETED GENETIC ALTERATION IN PLANT CELLS 审中-公开
公开(公告)号：WO2018115389A1
公开(公告)日：2018-06-28
申请号：PCT/EP2017/084291
申请日：2017-12-22
申请人： KEYGENE N.V.
发明人： BUNDOCK, Paul
IPC分类号： C12N15/82
CPC分类号： C12N15/8213 , C12Q2521/307
摘要： The current invention relates to methods of targeted genetic alteration in plant cells, as well as to plant cells and plants thus obtained. In the method, the use of the combination of a nickase and a single-stranded oligonucleotide of the invention, provides for targeted alteration of a DNA duplex with increased efficacy.

6. 发明申请

WO2014104878A1 METHOD FOR REMOVING GENETIC LINKAGE IN A PLANT 审中-公开
标题翻译：在植物中移除遗传连锁的方法
公开(公告)号：WO2014104878A1
公开(公告)日：2014-07-03
申请号：PCT/NL2013/050939
申请日：2013-12-20
申请人： KEYGENE N.V.
发明人： BUNDOCK, Paul , STUURMAN, Jeroen
IPC分类号： A01H1/00 , A01H1/04 , C12N9/22
CPC分类号： A01H1/00 , A01H1/04 , C07K2319/81 , C12N9/22 , C12N15/8241
摘要： The current disclosure relates to the field of plants, in particular to the fields of plant breeding and plant genetics. More particular, the disclosure concerns inventive methodology that may be useful in improving plant properties.In particular the invention may be useful in removing linkage drag. Also provided are plant and plant parts obtained with the method disclosed herein.
摘要翻译：本公开涉及植物领域，特别是植物育种和植物遗传学领域。更具体地，本公开涉及可用于改善植物性质的发明方法。特别地，本发明可用于除去连锁阻力。还提供了通过本文公开的方法获得的植物和植物部分。

7. 发明申请

WO2013058654A2 METHODS FOR PRODUCING CINNAMOLIDE AND/OR DRIMENDIOL 审中-公开
标题翻译：生产新戊酰胺和/或维生素B1的方法
公开(公告)号：WO2013058654A2
公开(公告)日：2013-04-25
申请号：PCT/NL2012/050729
申请日：2012-10-19
申请人： KEYGENE N.V.
发明人： BOUWMEESTER, Hendrik Jan , HENQUET, Maurice Gerard Leon , JONGSMA, Maarten Anthonie
IPC分类号： C12N9/00
CPC分类号： C12P7/02 , C12N9/0004 , C12N9/0006 , C12N9/16 , C12N15/8241 , C12N15/8286 , C12P17/04
摘要： Described is a nucleic acid sequence isolated from Persicaria hydropiper and encoding a drimenol oxidase protein, expression vectors comprising such nucleic acid sequence, chimeric genes comprising such nucleic acid sequence, host cells or host organisms altered to harbour the drimenol oxidase nucleic acid sequence, and the drimenol oxidase protein itself. Methods for producing cinnamolide and/or drimendiol and/or enhanced levels of cinnamolide and/or drimendiol, in a cell or organism harbouring such nucleic acid sequence are provided. Transgenic organisms comprising the nucleic acid sequence or a chimeric gene of the invention are also provided. The present invention especially relates to transgenic plants with enhanced resistance to insects and enhanced insect antifeedant properties.
摘要翻译：描述了从Persicaria hydropiper分离的核酸序列，并编码一种石蜡氧化酶蛋白，包含该核酸序列的表达载体，包含该核酸序列的嵌合基因，被改变为含有该二醇氧化酶核酸序列的宿主细胞或宿主生物，以及石蜡氧化酶蛋白本身。提供了在含有这种核酸序列的细胞或生物体中生产肉桂酸酯和/或烈im醇和/或增强的肉桂酸酯和/或烈end醇水平的方法。还提供了包含本发明的核酸序列或嵌合基因的转基因生物。本发明特别涉及具有增强的对昆虫的抗性和增强的昆虫抗食性质的转基因植物。

8. 发明申请

WO2013006058A1 USE OF JAZ5A FOR IMPROVING DROUGHT-RESISTANCE IN A PLANT 审中-公开
标题翻译：使用JAZ5A改善植物抗旱性
公开(公告)号：WO2013006058A1
公开(公告)日：2013-01-10
申请号：PCT/NL2012/050481
申请日：2012-07-05
申请人： KEYGENE N.V. , VAN TUNEN, Adrianus, Johannes
发明人： VAN TUNEN, Adrianus, Johannes
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C12N15/8273 , C07K14/415 , C12Q1/6895
摘要： The present invention provides use of a plant gene JAZ5a for improving drought-resistance of a plant. It further provides a method for improving the drought-resistance of a plant, comprising enhancing the expression or activity of Jaz5A in said plant.
摘要翻译：本发明提供植物基因JAZ5a用于改善植物抗旱性的用途。它进一步提供了一种改善植物抗旱性的方法，包括增强所述植物中Jaz5A的表达或活性。

9. 发明申请

WO2012096579A2 PAIRED END RANDOM SEQUENCE BASED GENOTYPING 审中-公开
标题翻译：基于对偶序列序列的基因分析
公开(公告)号：WO2012096579A2
公开(公告)日：2012-07-19
申请号：PCT/NL2012/050022
申请日：2012-01-13
申请人： KEYGENE N.V. , VAN EIJK, Michael, Josephus, Theresia
发明人： VAN EIJK, Michael, Josephus, Theresia
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6809 , C12Q1/683 , C12Q1/6869 , C12Q2525/151 , C12Q2535/119 , C12Q2535/138 , C12Q2563/179 , C12Q2565/543
摘要： Method for simultaneous discovery, detection and genotyping of polymorphisms between samples by providing identifier tagged restriction fragments, obtaining sequence information using paired high throughput sequencing technologies, combining the sequence information and identify polymorphisms between the samples. The combination of sequence information from both ends allows for the discovery, detection and genotyping of polymorphisms in highly repetitive genomes.
摘要翻译：通过提供标识符标记的限制性片段同时发现，检测和多样性基因分型的方法，使用配对的高通量测序技术获得序列信息，组合序列信息并鉴定样品之间的多态性。来自两端的序列信息的组合允许在高度重复的基因组中的多态性的发现，检测和基因分型。

10. 发明申请

WO2012074386A1 TARGETED ALTERATION OF DNA WITH OLIGONUCLEOTIDES 审中-公开
标题翻译： DNA与寡核苷酸的靶向修饰
公开(公告)号：WO2012074386A1
公开(公告)日：2012-06-07
申请号：PCT/NL2011/050805
申请日：2011-11-25
申请人： KEYGENE N.V. , DE BOTH, Michiel Theodoor Jan , FURUKAWA, Tomoyuki
发明人： DE BOTH, Michiel Theodoor Jan , FURUKAWA, Tomoyuki
IPC分类号： C12N15/10
CPC分类号： C12N15/1024 , C12N15/102 , C12N15/1082
摘要： The current invention relates to a method for targeted alteration of acceptor DNA, for example duplex acceptor DNA. The method comprises use of at least two oligonucleotides, each oligonucleotide having at least one mismatch relative to the targeted (duplex) acceptor DNA. The mismatch of the first oligonucleotide is directed to a nucleotide at a position in the first strand of the duplex and the mismatch of the second oligonucleotide is directed to the nucleotide in the second strand that occupies the complementary position in the duplex acceptor DNA (e.g. forms a base-pair with the nucleotide in the first strand). These mismatches are located at specific positions within said oligonucleotides. Also provided is a kit that comprises instructions for performing the method according to the inventions, and in a preferred embodiment, comprises oligonucleotides suitable for use in the method.
摘要翻译：本发明涉及用于靶向改变受体DNA的方法，例如双链受体DNA。该方法包括使用至少两种寡核苷酸，每种寡核苷酸相对于靶向（双链体）受体DNA具有至少一个失配。第一寡核苷酸的不匹配指向双链体第一链中位置的核苷酸，第二寡核苷酸的错配指向第二链中位于双链受体DNA中的互补位置的核苷酸（例如形式与第一链中的核苷酸的碱基对）。这些错配位于所述寡核苷酸内的特定位置。还提供了包括用于执行根据本发明的方法的指令的试剂盒，并且在优选实施方案中，包含适合用于该方法的寡核苷酸。

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