专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2004065573A2 NOVEL HIGH THROUGHPUT METHOD OF GENERATING AND PURIFYING LABELED CRNA TARGETS FOR GENE EXPRESSION ANALYSIS 审中-公开
标题翻译：用于基因表达分析的产生和纯化标记的CRNA靶的新型高通量方法
公开(公告)号：WO2004065573A2
公开(公告)日：2004-08-05
申请号：PCT/US2004/000538
申请日：2004-01-12
申请人： WYETH , LUCIANO, Joseph, Peter, Jr. , BROWN, Eugene, L.
发明人： LUCIANO, Joseph, Peter, Jr. , BROWN, Eugene, L.
IPC分类号： C12N
CPC分类号： C12P19/34
摘要： A method of generating substantially pure polynucleotides in a multiple-compartment container using a multiple-compartment purification filter is described. In particular, a method of generating cDNA or cRNA labeled with biotin for the ease of detection is described.
摘要翻译：描述了使用多室纯化过滤器在多室容器中产生基本上纯的多核苷酸的方法。特别地，描述了为了便于检测而生成用生物素标记的cDNA或cRNA的方法。

2. 发明申请

WO2005111246A1 OLIGONUCLEOTIDE ARRAYS TO MONITOR GENE EXPRESSION AND METHODS FOR MAKING AND USING SAME 审中-公开
标题翻译：寡核苷酸阵列监测基因表达及其制备和使用方法
公开(公告)号：WO2005111246A1
公开(公告)日：2005-11-24
申请号：PCT/US2005/016880
申请日：2005-05-11
申请人： WYETH , MELVILLE, Mark, W. , CHARLEBOIS, Timothy, S. , MOUNTS, William, M. , HANN, Louane, E. , SINACORE, Martin, S. , LEONARD, Mark, W. , BROWN, Eugene, L. , MILLER, Christopher, P. , LEE, Gene, W.
发明人： MELVILLE, Mark, W. , CHARLEBOIS, Timothy, S. , MOUNTS, William, M. , HANN, Louane, E. , SINACORE, Martin, S. , LEONARD, Mark, W. , BROWN, Eugene, L. , MILLER, Christopher, P. , LEE, Gene, W.
IPC分类号： C12Q1/68
CPC分类号： C07K14/47 , C12Q1/6837 , C12Q1/6876 , C12Q2600/158
摘要： The present invention provides an oligonucleotide array capable of identifying genes and related pathways involved with the induction of a particular phenotype by a cell line, e.g., the genes and related pathways involved with the induction of transgene expression by the cell line. The invention is particularly useful when there is little or no information about the genome of the cell line being studied, because it provides methods for identifying consensus sequences for known and previously undiscovered genes, and for designing oligonucleotide probes to the identified consensus sequences. Additionally, when the array is to be used to determine optimal conditions for expression of a transgene by the cell line, the invention teaches methods of including oligonucleotide probes to transgene sequences in the array. The invention also provides methods of using the array to identify genes and related pathways involved with the induction of a particular cell line phenotype. The invention also provides novel polynucleotides of undiscovered genes (i.e., a gene that had not been sequenced and/or shown to be expressed by CHO cells) and novel polynucleotides involved with the induction of a particular cell phenotype, e.g., increased survival when grown under stressful culture conditions, increased transgene expression, decreased production of an antigen, etc. These novel polynucleotides are termed novel CHO sequences and differential CHO sequences, respectively. The invention also provides genetically engineered expression vectors, host cells, and transgenic animals comprising the novel nucleic acid molecules of the invention. The invention additionally provides antisense and RNAi molecules to the nucleic acid molecules of the invention. The invention further provides methods of using the polynucleotides of the invention.
摘要翻译：本发明提供了能够鉴定涉及通过细胞系诱导特定表型的基因和相关途径的寡核苷酸阵列，例如涉及通过细胞系诱导转基因表达的基因和相关途径。当关于正在研究的细胞系的基因组的信息很少或没有信息时，本发明是特别有用的，因为它提供了用于鉴定已知和先前未被发现的基因的共有序列的方法，以及为所识别的共有序列设计寡核苷酸探针。另外，当该阵列用于确定由细胞系表达转基因的最佳条件时，本发明教导了将寡核苷酸探针包括在阵列中的转基因序列的方法。本发明还提供了使用该阵列鉴定涉及诱导特定细胞系表型的基因和相关途径的方法。本发明还提供未被发现的基因的新型多核苷酸（即，未被测序和/或显示由CHO细胞表达的基因）和参与诱导特定细胞表型的新型多核苷酸，例如当在下面生长时增加的存活压力培养条件，转基因表达增加，抗原产生减少等。这些新型多核苷酸分别被称为新的CHO序列和差异CHO序列。本发明还提供基因工程表达载体，宿主细胞和包含本发明的新型核酸分子的转基因动物。本发明还向本发明的核酸分子提供反义和RNAi分子。本发明还提供使用本发明的多核苷酸的方法。

3. 发明申请

WO2007106488A2 MODULATORS OF GLUCONEOGENESIS 审中-公开
标题翻译：葡萄糖生成的调节剂
公开(公告)号：WO2007106488A2
公开(公告)日：2007-09-20
申请号：PCT/US2007/006313
申请日：2007-03-13
申请人： WYETH , BERASI, Stephen , HUARD, Christine , LI, Dongmei , SHIH, Heather , SUN, Ying , PAULSEN, Janet , BROWN, Eugene, L. , GIMENO, Ruth , MARTINEZ, Robert
发明人： BERASI, Stephen , HUARD, Christine , LI, Dongmei , SHIH, Heather , SUN, Ying , PAULSEN, Janet , BROWN, Eugene, L. , GIMENO, Ruth , MARTINEZ, Robert
IPC分类号： A61K38/17 , A61K38/18 , A61K31/7088
CPC分类号： C07K14/4702 , A61K38/1709 , C12N9/16
摘要： The present invention provides methods of affecting gluconeogenesis by altering the levels or activity of DUSP4 and/or EGRl . Pharmaceutical compositions comprising or encoding modulators of gluconeogenesis are also disclosed.
摘要翻译：本发明提供通过改变DUSP4和/或EGR1的水平或活性来影响糖异生的方法。还公开了包含或编码糖异生的调节剂的药物组合物。

4. 发明申请

WO2006025879A2 NOVEL POLYNUCLEOTIDES RELATED TO OLIGONUCLEOTIDE ARRAYS TO MONITOR GENE EXPRESSION 审中-公开
标题翻译：与寡核苷酸阵列相关的新型多核苷酸监测基因表达
公开(公告)号：WO2006025879A2
公开(公告)日：2006-03-09
申请号：PCT/US2005/016425
申请日：2005-05-11
申请人： WYETH , MELVILLE, Mark, W. , CHARLEBOIS, Timothy, S. , MOUNTS, William, M. , HANN, Louane, E. , SINACORE, Martin, S. , LEONARD, Mark, W. , BROWN, Eugene, L. , MILLER, Christopher, P. , LEE, Gene, W.
发明人： MELVILLE, Mark, W. , CHARLEBOIS, Timothy, S. , MOUNTS, William, M. , HANN, Louane, E. , SINACORE, Martin, S. , LEONARD, Mark, W. , BROWN, Eugene, L. , MILLER, Christopher, P. , LEE, Gene, W.
IPC分类号： C12Q1/68
CPC分类号： C07K14/47 , C12Q1/6837 , C12Q1/6876 , C12Q2600/158
摘要： The present invention provides an oligonucleotide array capable of identifying genes and related pathways involved with the induction of a particular phenotype by a cell line, e.g., the genes and related pathways involved with the induction of transgene expression by the cell line. The invention is particularly useful when there is a little or no information about the genome of the cell line being studied, because it provides methods for identifying consensus sequences for known and previously undiscovered genes, and for designing oligonucleotide probes to the identified consensus sequences. Additionally, when the array is to be used to determine optimal conditions for expression of a transgene by the cell line, the invention teaches methods of including oligonucleotide probes to transgene sequences in the array. The invention also provides methods of using the array to identify genes and related pathways involved with the induction of a particular cell line phenotype. The invention also provides novel polynucleotides of undiscovered genes (i.e., a gene that had not been sequenced and/or shown to be expressed by CHO cells) and novel polynucleotides involved with the induction of a particular cell phenotype, e.g., increased survival when grown under stressful culture conditions, increased transgene expression, decreased production of an antigen, etc. These novel polynucleotides are termed novel CHO sequences and differential CHO sequences, respectively. The invention also provides genetically engineered expression vectors, host cells, and transgenic animals comprising the novel nucleic acid molecules of the invention. The invention additionally provides antisense and RNAi molecules to the nucleic acid molecules of the invention. The invention further provides methods of using the polynucleotides of the invention.
摘要翻译：本发明提供了能够鉴定涉及通过细胞系诱导特定表型的基因和相关途径的寡核苷酸阵列，例如涉及通过细胞系诱导转基因表达的基因和相关途径。当有关于正在研究的细胞系的基因组的信息很少或没有信息时，本发明是特别有用的，因为它提供了用于鉴定已知和先前未被发现的基因的共有序列的方法，以及为所识别的共有序列设计寡核苷酸探针。另外，当该阵列用于确定由细胞系表达转基因的最佳条件时，本发明教导了将寡核苷酸探针包括在阵列中的转基因序列的方法。本发明还提供了使用该阵列鉴定涉及诱导特定细胞系表型的基因和相关途径的方法。本发明还提供未被发现的基因（即，未被测序和/或显示由CHO细胞表达的基因）的新型多核苷酸以及参与特定细胞表型诱导的新型多核苷酸，例如当在下压力培养条件，转基因表达增加，抗原产生减少等。这些新型多核苷酸分别被称为新的CHO序列和差异CHO序列。本发明还提供了遗传工程改造的表达载体，宿主细胞和包含本发明的新型核酸分子的转基因动物。本发明还向本发明的核酸分子提供反义和RNAi分子。本发明还提供使用本发明的多核苷酸的方法。

5. 发明申请

WO2002099071A2 HYBRIDIZATION NORMALIZATION METHODS 审中-公开
标题翻译：混合正规化方法
公开(公告)号：WO2002099071A2
公开(公告)日：2002-12-12
申请号：PCT/US2002/017813
申请日：2002-06-06
申请人： GENE LOGIC, INC. , WYETH INCORPORATED , SCHERF, Uwe , ELASHOF, Michael , BEAZER-BARCLAY, Yasmin , ANTONELLIS, Kristen, J. , JELINSKY, Scott, A. , WHITLEY, Marriane , BROWN, Eugene, L.
发明人： SCHERF, Uwe , ELASHOF, Michael , BEAZER-BARCLAY, Yasmin , ANTONELLIS, Kristen, J. , JELINSKY, Scott, A. , WHITLEY, Marriane , BROWN, Eugene, L.
IPC分类号： C12N
CPC分类号： C12Q1/6832 , C12Q1/6837 , C12Q2545/113
摘要： The present invention includes methods of normalizing hybridization reactions that are designed to select normalization control genes, specifically 5'-3'-, and middle portions of the these genes, that hybridize similarly to a probe array and that produce the most consistently linear curve of hybridization signal over a range of normalization control gene segment concentrations. These methods have applicability across a broad spectrum of hybridization formats.
摘要翻译：本发明包括归一化杂交反应的方法，其被设计用于选择归一化对照基因，特别是这些基因的5'-3'-和中间部分，其与探针阵列类似地杂交并产生最一致的线性曲线杂交信号在一系列归一化控制基因片段浓度。这些方法在广泛的杂交格式中具有适用性。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式