专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2016189065A8 METHOD AND SYSTEM FOR ASSESSING STAIN QUALITY FOR IN-SITU HYBRIDIZATION AND IMMUNOHISTOCHEMISTRY 审中-公开
标题翻译：评估原位杂交和免疫组织化学质量的方法和系统
公开(公告)号：WO2016189065A8
公开(公告)日：2017-04-27
申请号：PCT/EP2016061859
申请日：2016-05-25
申请人： VENTANA MED SYST INC , F HOFFMANN-LA ROCHE AG
发明人： GARSHA KARL , OTTER MICHAEL , STEVENS BENJAMIN , TAFT JEFFERSON , VENTURA FRANK
IPC分类号： G06K9/00 , G01N1/30
CPC分类号： G06K9/00127 , G01N1/30
摘要： Immunohistochemistry (IHC) and in situ hybridization (ISH) have the aim of detecting, localizing and quantifying certain analytes for various diagnostic purposes. The quality of the stains which are analyzed may deviate for various reasons. Therefore, the present invention provides a method and system for assessing the stain quality and for establishing objective criteria for assessing the stain quality for application in the fields of in-situ hybridization and immunohistochemistry. In one possible embodiment, the invention comprises the steps of unmixing multi-spectral image data of a tissue specimen to obtain analyte intensity images, each analyte intensity image comprising signals from a single stain, computing metrics based on the analyte intensity images, wherein the metrics are uniformity, distribution and/or dispersion of pixel intensity values in the analyte intensity images and assessing a stain quality of a slide by comparing the computed metrics to pre-determined cutoff values regarding uniformity, distribution and/or dispersion of pixel intensity, wherein the stain quality of the slide is assessed as acceptable if the computed metrics meet or exceed the pre-determined cutoff values, and wherein the stain quality of the slide is assessed as unacceptable if the computed metrics do not meet the pre-determined cutoff values. In order to establish objective criteria for assessing stain quality, in one possible embodiment, the method and system includes the step of deriving cut-off values for uniformity, distribution and/or dispersion of pixel intensity by combining the computed metrics based on the analyte intensity images with pre-established data quantifying the stain quality.
摘要翻译：免疫组织化学（IHC）和原位杂交（ISH）具有检测，定位和定量某些分析物用于各种诊断目的的目的。由于各种原因，分析的污渍质量可能会有所偏差。因此，本发明提供了用于评估染色质量和建立用于评估染色质量的客观标准以用于原位杂交和免疫组织化学领域的方法和系统。在一个可能的实施例中，本发明包括以下步骤：将组织样本的多光谱图像数据解混以获得分析物强度图像，每个分析物强度图像包括来自单一染色物的信号，基于分析物强度图像计算度量，其中度量是分析物强度图像中的像素强度值的均匀性，分布和/或分散，并通过将计算出的度量与关于像素强度的均匀性，分布和/或分散的预定截止值进行比较来评估载玻片的染色质量，其中如果所计算的度量达到或超过预定的截断值，则将该载玻片的污点质量评估为可接受的，并且如果所计算的度量不满足预定的截断值，则将该载玻片的污点质量评估为不可接受。为了建立评估污渍质量的客观标准，在一个可能的实施例中，该方法和系统包括通过组合基于分析物强度计算的度量来获得像素强度的均匀性，分布和/或分散的截止值的步骤具有量化污渍质量的预先建立的数据的图像。

IPRDB

热门服务

关于我们

友情链接

联系方式