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    • 1. 发明申请
    • TARGETED FUSION PROTEINS AND METHODS FOR THE CHARACTERIZATION OF CELLULAR MEMBRANE DOMAINS
    • 有针对性的融合蛋白和细胞膜结构域特征的方法
    • WO2003006606A2
    • 2003-01-23
    • PCT/US2002/021495
    • 2002-07-09
    • OKLAHOMA MEDICAL RESEARCH FOUNDATIONRODGERS, William
    • RODGERS, William
    • C12N
    • C12N9/1205C07K14/705C07K2319/00C07K2319/02C07K2319/033C07K2319/60G01N33/5041G01N33/92
    • Cell membranes containing glycolipid-enriched membrane (GEM) and non-glycolipid-enriched membrane (non-GEM) domains are targeted using fusion proteins that are anchored in the cell membrane. Fusion proteins to target GEM (or non-GEM) domains are comprised of a selected fluorescent polypeptide, a membrane-targeting sequence of p56 lck (or pp60 c-Src for non GEM domains) and a linker inserted between the polypeptide and the membrane targeting sequence. Localization of fusion proteins in GEM and non-GEM domains is assessed using techniques including confocal microscopy, fluorescence-based techniques, and membrane fractionation. Using these techniques, compounds are screened for their effect on GEM and non-GEM domains of live cells. These fusion proteins therefore represent useful tools for studying subcellular trafficking and the function of discrete compartments in the plasma membrane.
    • 含有富含脂肪糖的膜(GEM)和非糖脂富集膜(非GEM)结构域的细胞膜使用锚定在细胞膜中的融合蛋白进行靶向。 靶向GEM(或非GEM)结构域的融合蛋白由选择的荧光多肽,p56 的膜靶向序列(或非GEM结构域的pp60 )和插入多肽 和膜靶向序列。 使用包括共焦显微镜,基于荧光的技术和膜分级技术的技术来评估GEM和非GEM结构域中融合蛋白的定位。 使用这些技术,筛选化合物对活细胞的GEM和非GEM结构域的影响。 因此,这些融合蛋白是用于研究亚细胞运输和质膜中离散隔室功能的有用工具。
    • 4. 发明申请
    • MEASUREMENT OF SEQUESTERED CARBON
    • 测定碳的测定
    • WO2009021269A1
    • 2009-02-19
    • PCT/AU2008/001148
    • 2008-08-08
    • OCEAN NOURISHMENT CORPORATION PTY LIMITEDRODGERS, William
    • RODGERS, William
    • G01N33/00
    • G06Q10/04G01N33/18Y02P90/84
    • Disclosed herein are methods and systems for determining an amount of carbon dioxide that can be sequestered from the atmosphere by a portion of an ocean. The methods or systems involve the following steps (a) determining which chemical element or elements would, when added to a photic zone of the portion, cause growth of a phytoplankton population in the portion; (b) determining a quantity of the chemical element or elements to be added to the photic zone whereby substantially all of the element or substantially all of at least one of the elements added would be consumed by the additional phytoplankton grown; and (c) calculating the amount of carbon dioxide that can be sequestered from the atmosphere based on the determined quantity of the chemical element or elements that would be added to the portion.
    • 本文公开了用于确定可由一部分海洋从大气隔离的二氧化碳的量的方法和系统。 方法或系统包括以下步骤(a)确定当加入到该部分的光泽区域时,哪个化学元素或元素会导致该部分中浮游植物群体的生长; (b)确定要添加到光区的化学元素的量,其中添加的元素中的至少一个元素或基本上全部的元素将被附加的浮游植物消耗; 和(c)基于确定的添加到该部分的化学元素的量来计算可从大气中隔离的二氧化碳的量。