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1. 发明申请

WO0228841B1 REAGENTS FOR LABELING BIOMOLECULES HAVING ALDEHYDE OR KETONE MOIETIES 审中-公开
标题翻译：用于标记含有二醛或酮酮生物分子的试剂
公开(公告)号：WO0228841B1
公开(公告)日：2002-11-21
申请号：PCT/US0130851
申请日：2001-10-02
申请人： MOLECULAR PROBES INC
发明人： HAUGLAND RICHARD P , STEINBERG THOMAS H , PATTON WAYNE P , ZHENJUN DIWU
IPC分类号： G01N33/532 , C07D239/91 , C07D239/94 , C07D249/08 , C07D263/60 , C07D265/22 , C07D277/66 , C07D401/04 , G01N33/58
CPC分类号： C07D265/22 , C07D239/91 , Y10T436/143333 , Y10T436/200833
摘要： Novel fluorescent derivitization reagents are described that are suitable for coupling to biomolecules that contain aldehyde or ketone functional groups. The method of treating a sample with the derivativization reagents is described. The reagents are particularly useful for labeling glycoproteins or glycopeptides, nucleic acids, and lipopolysaccharides in electrophoresis gels.
摘要翻译：描述了适用于与含有醛或酮官能团的生物分子偶联的新型荧光衍生试剂。描述了用衍生化试剂处理样品的方法。这些试剂特别可用于在电泳凝胶中标记糖蛋白或糖肽，核酸和脂多糖。

2. 发明申请

WO0228841A3 REAGENTS FOR LABELING BIOMOLECULES HAVING ALDEHYDE OR KETONE MOIETIES 审中-公开
标题翻译：用于标记具有醛或酮的生物分子的试剂
公开(公告)号：WO0228841A3
公开(公告)日：2002-08-01
申请号：PCT/US0130851
申请日：2001-10-02
申请人： MOLECULAR PROBES INC
发明人： HAUGLAND RICHARD P , STEINBERG THOMAS H , PATTON WAYNE P , ZHENJUN DIWU
IPC分类号： G01N33/532 , C07D239/91 , C07D239/94 , C07D249/08 , C07D263/60 , C07D265/22 , C07D277/66 , C07D401/04 , G01N33/58
CPC分类号： C07D265/22 , C07D239/91 , Y10T436/143333 , Y10T436/200833
摘要： Novel fluorescent derivitization reagents are described that are suitable for coupling to biomolecules that contain aldehyde or ketone functional groups. The method of treating a sample with the derivativization reagents is described. The reagents are particularly useful for labeling glycoproteins or glycopeptides, nucleic acids, and lipopolysaccharides in electrophoresis gels.
摘要翻译：描述了适用于与含有醛或酮官能团的生物分子偶联的新的荧光衍生试剂。描述了用衍生化试剂处理样品的方法。这些试剂特别适用于标记电泳凝胶中的糖蛋白或糖肽，核酸和脂多糖。

3. 发明申请

WO0025139B1 LUMINESCENT PROTEIN STAINS CONTAINING TRANSITION METAL COMPLEXES 审中-公开
标题翻译：含有过渡金属络合物的发光蛋白染料
公开(公告)号：WO0025139B1
公开(公告)日：2000-07-06
申请号：PCT/US9925349
申请日：1999-10-27
申请人： MOLECULAR PROBES INC
发明人： BHALGAT MAHESH K , DIWU ZHENJUN , HAUGLAND RICHARD P , PATTON WAYNE F
IPC分类号： G01N31/22 , G01N1/10 , G01N21/78 , G01N27/447 , G01N31/00 , G01N33/533 , G01N33/58 , G01N33/68
CPC分类号： G01N33/6803 , G01N33/533 , G01N33/582 , G01N33/68 , G01N33/683 , G01N33/6845 , G01N33/6851 , Y10T436/25125 , Y10T436/25375
摘要： The invention relates to the staining of poly(amino acids), including peptides, polypeptides and proteins in gels and on solid supports, using neutral or anionic complexes of transition metals.
摘要翻译：本发明涉及使用过渡金属的中性或阴离子络合物染色聚（氨基酸），包括凝胶和固体载体上的肽，多肽和蛋白质。

4. 发明申请

WO03030817B1 ANTIBODY COMPLEXES AND METHODS FOR IMMUNOLABELING 审中-公开
标题翻译：抗体复合物和免疫标记方法
公开(公告)号：WO03030817B1
公开(公告)日：2003-11-06
申请号：PCT/US0231416
申请日：2002-10-02
申请人： MOLECULAR PROBES INC , ARCHER ROBERT A , BEECHEM JOSEPH M , HAGEN DAVID C , HAUGLAND RICHARD P , HAUGLAND ROSARIA P
发明人： ARCHER ROBERT A , BEECHEM JOSEPH M , HAGEN DAVID C , HAUGLAND RICHARD P , HAUGLAND ROSARIA P
IPC分类号： G01N33/53 , G01N33/58 , G01N33/68
CPC分类号： B82Y30/00 , B82Y5/00 , B82Y10/00 , G01N33/53 , G01N33/58 , G01N33/6857
摘要： The present invention provides labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling proteins have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Typically, the labeling reagent is an anti-Fc Fab or Fab' fragment that was generated by immunizing a goat or rabbit with the Fc fragment of an antibody. The present invention provides for discrete subsets of labeling reagent and immuno-labeled complexes that facilitate the simultaneous detection of multiple targets in a sample wherein the immuno-labeled complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeling reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.
摘要翻译：本发明提供标记试剂和用于标记一抗的标记试剂和使用包含靶结合抗体和一种或多种标记试剂的免疫标记复合物检测样品中靶标的方法。标记试剂包括单价抗体片段或非抗体单体蛋白质，由此标记的蛋白质对靶标结合抗体的特定区域具有亲和性，并且共价连接到标记物上。通常，标记试剂是通过用抗体的Fc片段免疫山羊或兔而产生的抗Fc Fab或Fab'片段。本发明提供标记试剂和免疫标记的复合物的离散子集，其有助于同时检测样品中的多个靶标，其中通过i）标记物与标记试剂的比率来区分免疫标记的复合物，或ii）物理或iii）标记试剂与所述靶结合抗体的比例，或iv）所述靶结合抗体。这对于可以连接到标记试剂和随后的用于检测多个靶的比例的免疫标记的复合物的荧光团标记物特别有用。

5. 发明申请

WO9613552A3 SUBSTITUTED UNSYMMETRICAL CYANINE DYES WITH SELECTED PERMEABILITY 审中-公开
标题翻译：具有选择性渗透性的取代的不对称氰基染料
公开(公告)号：WO9613552A3
公开(公告)日：1996-07-11
申请号：PCT/US9513706
申请日：1995-10-27
申请人： MOLECULAR PROBES INC
发明人： YUE STEPHEN T , SINGER VICTORIA L , ROTH BRUCE L , MOZER THOMAS J , MILLARD PAUL J , JONES LAURIE J , JIN XIAOKUI , HAUGLAND RICHARD P , POOT MARTIN
IPC分类号： G01N33/48 , C07D215/18 , C07D413/06 , C07D417/06 , C07D417/14 , C09B23/00 , C09B23/02 , C12Q1/68 , G01N21/76 , G01N33/58
CPC分类号： C07D417/14 , C07D215/18 , C07D413/06 , C07D417/06 , C09B23/02 , C12Q1/6816 , G01N15/1459 , G01N2015/1402 , Y10S436/80 , Y10T436/143333 , C12Q2563/173
摘要： The invention describes the preparation and use of fluorescent stains for nucleic acids derived from unsymmetrical cyanine dyes comprising a substituted benzazolium ring system linked by a methine bridge to a pyridinium or quinolinium ring system. The cyanine dyes of the invention possess a high sensitivity to oligonucleotides and larger nucleic acid polymers in a wide range of cells and gels, and are useful for the analysis of cell structure, membrane integrity or function, and determination of cell cycle distribution. 00000
摘要翻译：本发明描述了衍生自不对称花青染料的核酸的荧光染料的制备和用途，其包含通过次甲基桥与吡啶鎓或喹啉环体系连接的取代的苯并唑鎓环系统。本发明的花青染料在宽范围的细胞和凝胶中对寡核苷酸和较大的核酸聚合物具有高度敏感性，并且可用于细胞结构，膜完整性或功能的分析以及细胞周期分布的确定。 00000

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