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1. 发明申请

WO2011033005A2 CELLS FOR TRANSIENT EXPRESSION AND USES THEREOF 审中-公开
标题翻译：用于瞬时表达的细胞及其用途
公开(公告)号：WO2011033005A2
公开(公告)日：2011-03-24
申请号：PCT/EP2010/063583
申请日：2010-09-15
申请人： MEDIMMUNE LIMITED , DARAMOLA, Olalekan , DEAN, Gregory , HATTON, Diane , STEVENSON, Jessica
发明人： DARAMOLA, Olalekan , DEAN, Gregory , HATTON, Diane , STEVENSON, Jessica
IPC分类号： C12N5/10
CPC分类号： C12N7/00 , C07K14/005 , C07K16/00 , C07K2317/14 , C12N2710/16222
摘要： This invention relates to the transient expression of heterologous polypeptides in mammalian cell lines. Specifically it relates to an expression-enhanced cell line derived from a parent cell line, the expression-enhanced cell line comprising nucleic acid encoding Epstein-Barr Virus Nuclear Antigen 1 or a functional derivative, analogue, or variant thereof; and further comprising: (a) a nucleic acid encoding an exogenous glutamine synthetase; (b) a nucleic acid encoding an endogenous glutamine synthetase, wherein the endogenous glutamine is arranged to have enhanced enzymatic activity and/or enhanced expression relative to the parent cell line under comparable conditions; or (c) both (a) and (b).
摘要翻译：本发明涉及异源多肽在哺乳动物细胞系中的瞬时表达。具体地说，本发明涉及源自亲代细胞系的表达增强细胞系，该表达增强细胞系包含编码爱泼斯坦 - 巴尔病毒核抗原1的核酸或其功能衍生物，类似物或变体; 并且还包括：（a）编码外源谷氨酰胺合成酶的核酸; （b）编码内源性谷氨酰胺合成酶的核酸，其中所述内源性谷氨酰胺排列为在可比较的条件下相对于亲本细胞系具有增强的酶活性和/或增强的表达; 或（c）（a）和（b）。

2. 发明申请

WO2022123519A1 HYDROGEN PEROXIDE EVOLVED HOST CELL 审中-公开
公开(公告)号：WO2022123519A1
公开(公告)日：2022-06-16
申请号：PCT/IB2021/061570
申请日：2021-12-10
申请人： MEDIMMUNE LIMITED [GB]/[GB]
发明人： MISTRY, Rajesh Kumar , KELSALL, Emma Jane , SOU, Si Nga , HATTON, Diane , GIBSON, Suzanne , JENNS, Michal , WILLIS, Katie
IPC分类号： C07K16/00 , C12N5/10 , C12Q1/30
摘要： A directed evolution method for improving performance of a mammalian host cell is provided, as well as host cells generated using the directed evolution method. In one aspect, hydrogen peroxide (H2O2)-evolved host cells are provided. In one aspect, (H2O2)-evolved Chinese hamster ovary (CHO) host cells are provided.

3. 发明申请

WO2016113217A1 IL-13 BINDING PROTEINS AND USES THEREOF 审中-公开
标题翻译： IL-13结合蛋白及其用途
公开(公告)号：WO2016113217A1
公开(公告)日：2016-07-21
申请号：PCT/EP2016/050373
申请日：2016-01-11
申请人： MEDIMMUNE LIMITED
发明人： CARMEN, Sara , LOWE, David , GARDENER, Matthew, J , REES, Gareth, D , STRICKLAND, Ian , MAY, Richard, D , SENUSSI, Tarik , POPOVIC, Bojana , XU, Jianqing , GIBSON, Suzanne, J , HATTON, Diane
IPC分类号： C07K16/24
CPC分类号： C07K16/244 , A61K51/1021 , A61K2039/505 , A61K2039/507 , A61K2039/545 , C07K16/2866 , C07K2317/21 , C07K2317/24 , C07K2317/31 , C07K2317/33 , C07K2317/34 , C07K2317/52 , C07K2317/54 , C07K2317/55 , C07K2317/56 , C07K2317/565 , C07K2317/567 , C07K2317/622 , C07K2317/626 , C07K2317/64 , C07K2317/73 , C07K2317/76 , C07K2317/92 , C07K2317/94 , C07K2319/30 , C07K2319/55
摘要： Novel anti-IL-13 antigen-binding proteins such as antibodies and antigen-binding fragments thereof are provided. Methods of using the proteins to reduce IL-13 activity and to treat IL-13- associated diseases and conditions are further provided.
摘要翻译：提供新的抗IL-13抗原结合蛋白，如抗体及其抗原结合片段。进一步提供使用蛋白质降低IL-13活性和治疗IL-13相关疾病和病症的方法。

4. 发明申请

WO2022123519A2 HYDROGEN PEROXIDE EVOLVED HOST CELL 审中-公开
公开(公告)号：WO2022123519A2
公开(公告)日：2022-06-16
申请号：PCT/IB2021/061570
申请日：2021-12-10
申请人： MEDIMMUNE LIMITED
发明人： MISTRY, Rajesh Kumar , KELSALL, Emma Jane , SOU, Si Nga , HATTON, Diane , GIBSON, Suzanne , JENNS, Michal , WILLIS, Katie
IPC分类号： C07K16/00 , C12N5/10 , C12Q1/30 , C07K2317/14 , C07K2317/31 , C12N2510/00
摘要： A directed evolution method for improving performance of a mammalian host cell is provided, as well as host cells generated using the directed evolution method. In one aspect, hydrogen peroxide (H2O2)-evolved host cells are provided. In one aspect, (H2O2)-evolved Chinese hamster ovary (CHO) host cells are provided.

5. 发明申请

WO2018193072A1 IN SILICO DESIGN OF MAMMALIAN PROMOTERS WITH USER-DEFINED FUNCTIONALITY 审中-公开
公开(公告)号：WO2018193072A1
公开(公告)日：2018-10-25
申请号：PCT/EP2018/060125
申请日：2018-04-19
申请人： MEDIMMUNE LIMITED
发明人： BROWN, Adam, J , JAMES, David, C , GIBSON, Suzanne, J , HATTON, Diane
IPC分类号： C12N15/10 , C12Q1/6897
摘要： Simplified models of gene regulation are a fundamental requirement for mammalian synthetic biology. However, at the transcriptional level this has been impeded by the complex design rules governing promoter activity dynamics, preventing de novo -design of regulatory elements with user-defined functionalities. As demonstrated herein, when composite transcription factor binding sites do not function synergistically, mammalian promoters can be constructed according to simple design rules. Host-cell transcriptional machinery components were analyzed in silico to identify transcription factors with desired expression dynamics. Cognate binding sites were then comprehensively tested in homotypic and heterotypic architectures to assess modularity and determine the transcriptional activity exhibited by a single copy of each site. When elements were specifically selected to prevent combinatorial interactions, heterotypic promoter activities could be accurately modeled simply as a function of constituent binding site copy numbers. As binding site order, spacing, and orientation had minimal effect on promoter activity, blocks could be optimally combined and arranged in silico according to context-specific design-criteria. To demonstrate this, CHO cell promoters were created de-novo that exhibited designed activity levels and long-term expression stability in vitro . The findings reveal new insights into eukaryotic transcriptional regulatory mechanisms, and provide novel tools for mammalian synthetic biology.

6. 发明申请

WO2017072310A1 PREVENTION OF N-TERMINAL TRUNCATION IN IGG LIGHT CHAINS 审中-公开
标题翻译：预防IGG轻链中的N端截断
公开(公告)号：WO2017072310A1
公开(公告)日：2017-05-04
申请号：PCT/EP2016/076108
申请日：2016-10-28
申请人： MEDIMMUNE LIMITED
发明人： BOND, Nicholas, J , GIBSON, Suzanne, J , HATTON, Diane , HIGAZI, Daniel, R , MILNE, Sarah , SHERIFF, Mariana, Alessia
IPC分类号： C07K16/00
CPC分类号： C07K16/00 , C07K16/18 , C07K2317/515 , C07K2317/94 , C07K2319/02
摘要： We discovered that recombinant antibody light chains having a murine secretory leader sequence and an SYE motif at the N-terminus are truncated during post-translational processing. This disclosure provides two protein engineering solutions: to alter the SYE amino acid sequence of the Lc N-terminus to other alternatives; or to change the secretory leader peptide sequence. We have shown that both of these solutions are effective for preventing N-terminal light chain truncation.
摘要翻译：我们发现在翻译后加工过程中截短了在N-末端具有鼠分泌前导序列和SYE基序的重组抗体轻链。本公开提供了两种蛋白质工程解决方案：将Lc N端的SYE氨基酸序列改变为其他替代物; 或改变分泌前导肽序列。我们已经表明，这两种解决方案对于防止N端轻链截断都是有效的。

7. 发明申请

WO2012035079A1 CHINESE HAMSTER OVARY CELL LINE 审中-公开
标题翻译：中华肝细胞系
公开(公告)号：WO2012035079A1
公开(公告)日：2012-03-22
申请号：PCT/EP2011/065966
申请日：2011-09-14
申请人： HATTON, Diane , FIELD, Ray , FORREST-OWEN, Wyn , CROOK, Victoria , MEDIMMUNE LIMITED
发明人： HATTON, Diane , FIELD, Ray , FORREST-OWEN, Wyn , CROOK, Victoria
IPC分类号： C12N5/16
CPC分类号： C12N15/85 , C12N5/0682 , C12N2510/02 , C12N2511/00 , C12P21/00
摘要： The present invention is related to a Chinese hamster ovary cell line as deposited with the European Collection of Cell Cultures (ECACC) under accession number 10090201; use of the cell line for the production of a recombinant polypeptide; a kit comprising the cell line; and methods for the production of recombinant polypeptide.
摘要翻译：本发明涉及保藏在欧洲细胞培养物库（ECACC）上的中国仓鼠卵巢细胞系，登录号为10090201; 使用细胞系生产重组多肽; 包含细胞系的试剂盒; 以及生产重组多肽的方法。

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