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1. 发明申请

WO2012083189A2 METHODS, COMPOSITIONS, SYSTEMS, APPARATUSES AND KITS FOR NUCLEIC ACID AMPLIFICATION 审中-公开
标题翻译：用于核酸扩增的方法，组合物，系统，装置和试剂盒
公开(公告)号：WO2012083189A2
公开(公告)日：2012-06-21
申请号：PCT/US2011/065535
申请日：2011-12-16
申请人： LIFE TECHNOLOGIES CORPORATION , LI, Bin , LAO, Kai Qin , O'NEIL, Jennifer , KUNKEL, Jennifer , HALEY, Kellie , KASINSKAS, Rachel , MA, Zhaochun , BRZOSKA, Pius
发明人： LI, Bin , LAO, Kai Qin , O'NEIL, Jennifer , KUNKEL, Jennifer , HALEY, Kellie , KASINSKAS, Rachel , MA, Zhaochun , BRZOSKA, Pius
IPC分类号： C12Q1/68
CPC分类号： C12Q1/46 , C12P19/34 , C12Q1/6834 , C12Q1/6846 , C12Q1/6853 , C12Q1/6869 , C12Q1/6874 , C12Q2531/119 , C12Q2565/537
摘要： Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
摘要翻译：提供了在支持物上产生固定化克隆扩增子的局部群体的新方法。

2. 发明申请

WO2013158313A1 NUCLEIC ACID AMPLIFICATION 审中-公开
标题翻译：核酸放大
公开(公告)号：WO2013158313A1
公开(公告)日：2013-10-24
申请号：PCT/US2013/032598
申请日：2013-03-15
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： LI, Chieh-Yuan , RUFF, David , CHEN, Shiaw-Min , O'NEIL, Jennifer , KASINSKAS, Rachel , ROTHBERG, Jonathan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q2521/507 , C12Q2527/146 , C12Q2535/122 , C12Q2563/159 , C12Q2565/537 , C12Q2565/607
摘要： In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming an reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of an reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.
摘要翻译：在一些实施方案中，本发明提供核酸扩增方法，包括形成反应混合物，并使反应混合物经受适合于核酸扩增的条件。在一些实施方案中，用于核酸扩增的方法包括将待扩增的核酸进行部分变性的条件。在一些实施方案中，用于核酸扩增的方法包括扩增而不完全变性所扩增的核酸。在一些实施方案中，用于核酸扩增的方法使用催化同源重组的酶和聚合酶。在一些实施方案中，用于核酸扩增的方法可以在单个反应容器中进行。在一些实施方案中，用于核酸扩增的方法可以在反应混合物的单个连续液相中进行，而不需要分隔反应混合物或固定反应组分。在一些实施方案中，用于核酸扩增的方法包括在等温扩增条件下任选在聚合物存在下在表面上扩增至少一种多核苷酸。聚合物可以包括筛分剂和/或扩散还原剂。

3. 发明申请

WO2014031163A1 METHODS, COMPOSITIONS, SYSTEMS, APPARATUSES AND KITS FOR NUCLEIC ACID PAIRED END SEQUENCING 审中-公开
标题翻译：方法，组成，系统，核酸配对结束顺序的装置和工具
公开(公告)号：WO2014031163A1
公开(公告)日：2014-02-27
申请号：PCT/US2013/031589
申请日：2013-03-14
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： LI, Bin , LAO, Kai Qin , O'NEIL, Jennifer , KUNKEL, Jennifer , HALEY, Kellie , KASINSKAS, Rachel , MA, Zhaochun , BRZOSKA, Pius
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q2535/119 , C12Q2535/122 , C12Q2537/149 , C12Q2565/537
摘要： In some embodiments, the present teachings provide methods for paired end sequencing. In some embodiment, a polynucleotide template to be subjected to paired end sequencing comprises at least one cross linking moiety and at least one scissile moiety. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cleavage step, and (c) a reverse sequencing step. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cross-linking step, (c) a cleavage step, and (d) a reverse sequencing step.
摘要翻译：在一些实施方案中，本教导提供了用于配对末端测序的方法。在一些实施方案中，待进行配对末端测序的多核苷酸模板包含至少一个交联部分和至少一个可剪切部分。在一些实施方案中，配对末端测序反应包括（a）正向测序步骤，（b）切割步骤，和（c）反向测序步骤。在一些实施方案中，配对末端测序反应包括（a）正向测序步骤，（b）交联步骤，（c）切割步骤和（d）反向测序步骤。

4. 发明申请

WO2014043143A1 NUCLEIC ACID AMPLIFICATION 审中-公开
标题翻译：核酸放大
公开(公告)号：WO2014043143A1
公开(公告)日：2014-03-20
申请号：PCT/US2013/059093
申请日：2013-09-10
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： LI, Chieh-Yuan , RUFF, David , CHEN, Shiaw-Min , O'NEIL, Jennifer , KASINSKAS, Rachel , ROTHBERG, Jonathan , HINZ, Wolfgang
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2521/507 , C12Q2527/119 , C12Q2535/122 , C12Q2565/537 , C12Q2565/607
摘要： In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.
摘要翻译：在一些实施方案中，本发明提供核酸扩增方法，包括形成反应混合物，并使反应混合物经受适合于核酸扩增的条件。在一些实施方案中，用于核酸扩增的方法包括将待扩增的核酸进行部分变性的条件。在一些实施方案中，用于核酸扩增的方法包括扩增而不完全变性扩增的核酸。在一些实施方案中，用于核酸扩增的方法使用催化同源重组的酶和聚合酶。在一些实施方案中，用于核酸扩增的方法可以在单个反应容器中进行。在一些实施方案中，用于核酸扩增的方法可以在反应混合物的单个连续液相中进行，而不需要分隔反应混合物或固定反应组分。在一些实施方案中，用于核酸扩增的方法包括在等温扩增条件下任选地在聚合物存在下在表面上扩增至少一种多核苷酸。聚合物可以包括筛分剂和/或扩散还原剂。

5. 发明申请

WO2012138926A1 METHODS AND KITS FOR BREAKING EMULSIONS 审中-公开
标题翻译：破乳乳液的方法和工具
公开(公告)号：WO2012138926A1
公开(公告)日：2012-10-11
申请号：PCT/US2012/032425
申请日：2012-04-05
申请人： LIFE TECHNOLOGIES CORPORATION , SABINA, Jeffrey , ZLATKOVSKY, Ilya , KASINSKAS, Rachel
发明人： SABINA, Jeffrey , ZLATKOVSKY, Ilya , KASINSKAS, Rachel
IPC分类号： C12Q1/68
CPC分类号： C07H21/00 , C07K1/145 , C12Q1/6806 , C12Q1/6874 , G01N1/4055 , C12Q2523/32 , C12Q2563/161 , C12Q2535/122
摘要： The disclosure relates generally to methods, systems, compositions and kits for breaking a water-in-oil emulsion including one or more biomolecules dispersed in an aqueous phase of the water-in-oil emulsion. In some embodiments, the disclosure relates to obtaining a first emulsion including a continuous hydrophobic fraction and a discontinuous aqueous fraction, the aqueous fraction having one or more biomolecules dispersed therein, breaking the first emulsion by contacting the first emulsion with a breaking solution including a second emulsion, where the second emulsion includes a discontinuous phase of organic extraction solvent dispersed in a continuous aqueous phase, and centrifuging to separate the phases of the resulting mixture. In some embodiments, the disclosure relates generally to methods, kits and systems for extracting biomolecules from a water-in-oil emulsion, including breaking a water-in-oil emulsion comprising a plurality of aqueous droplets in a continuous hydrophobic fraction using a breaking solution to produce a resulting reaction mixture containing one or more biomolecules and manipulating the resulting reaction mixture to form at least two phases, where one of the phases includes an aqueous phase containing the one or more biomolecules.
摘要翻译：本公开一般涉及用于破坏油包水乳液的方法，系统，组合物和试剂盒，其包括分散在油包水乳液的水相中的一种或多种生物分子。在一些实施方案中，本公开涉及获得包含连续疏水部分和不连续含水部分的第一乳液，所述水性部分具有分散在其中的一种或多种生物分子，通过使第一乳液与包含第二乳液乳液，其中第二乳液包含分散在连续水相中的有机萃取溶剂的不连续相，并离心以分离所得混合物的相。在一些实施方案中，本公开一般涉及用于从油包水乳液中提取生物分子的方法，试剂盒和系统，包括使用破碎溶液破坏包含连续疏水部分中的多个水滴的油包水乳液以产生所得到的含有一种或多种生物分子的反应混合物并操纵所得反应混合物以形成至少两相，其中一相包括含有一种或多种生物分子的水相。

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