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1. 发明申请

WO2018093898A1 ULTRASPECIFIC NUCLEIC ACID SENSORS FOR LOW-COST LIQUID BIOPSIES 审中-公开
公开(公告)号：WO2018093898A1
公开(公告)日：2018-05-24
申请号：PCT/US2017/061796
申请日：2017-11-15
申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
发明人： GREEN, Alexander , HONG, Fan , YAN, Hao , CHAUDHARY, Soma , TANG, Anli
IPC分类号： C12N15/113 , C12Q1/68
摘要： Ultraspecific, programmable nucleic acid sensors capable of detecting and preferentially amplifying target DNA molecules comprising a particular SNP or mutation are provided. In some cases, the ultraspecific programmable nucleic acid sensors are useful for detecting SNP-containing DNA molecules indicative of cancer such as cell-free DNA circulating in the blood or indicative of organ transplant rejection Also provided are methods for construction of such ultraspecific nucleic acid sensors and methods for preferential amplification of target DNA molecules containing a mutation of interest, as well as testing systems for early cancer screening and routine monitoring of circulating cancer DNA using liquid biological samples such as serum, plasma, or saliva.

2. 发明申请

WO2018075502A1 UNIMOLECULAR APTAMER-BASED SENSORS FOR PATHOGEN DETECTION 审中-公开
标题翻译：基于单分子APTAMER的传感器用于病原检测
公开(公告)号：WO2018075502A1
公开(公告)日：2018-04-26
申请号：PCT/US2017/056960
申请日：2017-10-17
申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
发明人： GREEN, Alexander , MA, Duo , TANG, Anli
IPC分类号： C12N1/19 , C12N15/09 , C12N15/63 , C12N15/79
摘要： Methods and compositions for detecting the presence of a pathogen are described. In particular, this document provides a method of detecting a pathogen-associated nucleic acid in a biological sample of a subject, where the method comprises using one or more unimolecular aptamer-based sensors comprising an aptamer-fluorophore complex and an amplification step to detect the pathogen-associated nucleic acid. Methods specific for detecting the presence of malaria and other mosquito-borne virus infections are also provided.
摘要翻译：描述了用于检测病原体存在的方法和组合物。具体而言，该文件提供了检测受试者的生物样品中的病原体相关核酸的方法，其中该方法包括使用一种或多种包含适体 - 荧光团复合物的单分子适体基传感器和扩增步骤来检测病原体相关核酸。还提供了专门用于检测疟疾和其他蚊虫传播病毒感染的方法。

3. 发明申请

WO2022046978A2 PORTABLE DETECTION OF SARS-COV-2 USING UNIMOLECULAR APTASENSORS 审中-公开
公开(公告)号：WO2022046978A2
公开(公告)日：2022-03-03
申请号：PCT/US2021/047675
申请日：2021-08-26
申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
发明人： GREEN, Alexander , YAN, Zhaoqing , TANG, Anli
IPC分类号： C12Q1/04 , C12Q1/37 , G01N21/64 , C12Q1/68 , C12Q1/70
摘要： Provided herein are methods and compositions for rapid, highly sensitive detection of SARS-CoV-2, the causative agent of the COVID-19 pandemic or other target nucleic acids. The methods are low-cost and can be implemented in a portable format that does not require elaborate biosafety precautions or sophisticated laboratory equipment.

4. 发明申请

WO2021178557A1 RATIONAL DESIGN OF UPSTREAM ENHANCEMENT RNA FOR CIRCUIT DYNAMICS REGULATION AND VIRAL DIAGNOSTICS OPTIMIZATION 审中-公开
公开(公告)号：WO2021178557A1
公开(公告)日：2021-09-10
申请号：PCT/US2021/020698
申请日：2021-03-03
申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
发明人： ZHANG, Qi , WANG, Xiao , GREEN, Alexander , MA, Duo , WU, Fuqing , STANDAGE-BEIER, Kylie , CHEN, Xingwen
IPC分类号： C12N15/11 , C12N15/113
摘要： The present invention provides a new class of RNA modules, referred to as degradation tuning RNAs (dtRNAs), which form stabilizing secondary structures. Also provided are methods of using dtRNAs to modulate the stability of RNAs. DNA constructs including a promoter that is operably connected to a sequence encoding the dtRNA are also provided.

5. 发明申请

WO2018027177A1 LOOP-MEDIATED SYNTHETIC RIBOREGULATORS 审中-公开
标题翻译：环形介质合成弹性体
公开(公告)号：WO2018027177A1
公开(公告)日：2018-02-08
申请号：PCT/US2017/045585
申请日：2017-08-04
申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
发明人： GREEN, Alexander , MA, Duo
IPC分类号： C12N15/113 , C12N15/63 , C12N15/67 , C12Q1/68
CPC分类号： C12Q1/6897 , C12N15/113 , C12N15/63 , C12N15/67 , C12N2310/531 , C12Q1/68 , C12Q1/6809 , C12Q1/6816 , C12Q1/689 , C12Q1/70
摘要： Provided herein are synthetic nucleic acid molecules known as loop-mediated riboregulators that have single-nucleotide polymorphism (SNP) sensitivity and ultralow OFF state signal levels. Loop-mediated riboregulators can activate or repress gene expression in response to trigger RNAs bearing completely arbitrary sequences. Also provided herein are methods of using such synthetic nucleic acid molecules for detecting the presence or absence of a particular target RNA in, for example, a biological sample.
摘要翻译：本文提供被称为环介导的核糖核酸调节剂的合成核酸分子，其具有单核苷酸多态性（SNP）敏感性和超低OFF状态信号水平。响应于触发完全任意序列的RNA，环介导的核糖核酸调节剂可激活或抑制基因表达。本文还提供了使用此类合成核酸分子来检测例如生物样品中特定靶RNA的存在或不存在的方法。

6. 发明申请

WO2018026765A1 SYNTHETIC NEAR-THRESHOLD TRANSLATIONAL REPRESSORS 审中-公开
标题翻译：合成接近阈值的平移抑制器
公开(公告)号：WO2018026765A1
公开(公告)日：2018-02-08
申请号：PCT/US2017/044815
申请日：2017-08-01
申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
发明人： GREEN, Alexander , ZHOU, Yu
IPC分类号： C12N15/113 , C12N15/67 , C12N5/10 , C12Q1/68
CPC分类号： C12N15/113 , C12N15/67 , C12N2310/122 , C12N2310/531 , H03K19/20
摘要： Provided herein are synthetic nucleic acid molecules and methods of using such synthetic nucleic acid molecules for strong repression of target gene expression. In particular, provided herein are methods for altering expression of a protein in a cell, where the method comprises introducing into a cell a protein coding sequence operably linked to a near-threshold translational repressor having first and second trigger recognition sequences that are fully or partially complementary to a repressing trigger RNA; and introducing into a cell the repressing trigger RNA.
摘要翻译：本文提供了合成的核酸分子以及使用此类合成核酸分子强烈抑制靶基因表达的方法。具体而言，本文提供了用于改变细胞中蛋白质表达的方法，其中所述方法包括向蛋白质编码序列导入蛋白质编码序列，所述蛋白质编码序列可操作地连接至具有完全或部分第一和第二触发识别序列的近阈值翻译阻遏物与抑制性触发RNA互补; 并将抑制触发RNA导入细胞。

7. 发明申请

WO2017205668A1 PORTABLE, LOW-COST PATHOGEN DETECTION AND STRAIN IDENTIFICATION PLATFORM 审中-公开
标题翻译：便携式，低成本的病原体检测和菌株鉴定平台
公开(公告)号：WO2017205668A1
公开(公告)日：2017-11-30
申请号：PCT/US2017/034545
申请日：2017-05-25
申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY , PRESIDENT AND FELLOWS OF HARVARD COLLEGE , MASSACHUSETTS INSTITUTE OF TECHNOLOGY
发明人： GREEN, Alexander , BRAFF, Dana , TAKAHASHI, Melissa, K. , PARDEE, Keith , COLLINS, James, J. , LAMBERT, Guillaume , FERRANTE, Thomas
IPC分类号： C12N15/10 , C12N15/63 , C12N15/90 , C12Q1/68
摘要： Methods for detecting the presence of a pathogen infection are described. In particular, this document provides a method of detecting target nucleic acids, such as pathogen-specific RNA, in a biological sample obtained from a subject, where the method comprises using one or more toehold switch sensors and an isothermal amplification step to detect the target nucleic acid. Methods specific for detecting and identify the presence of a virus such as Zika virus are also provided.
摘要翻译：描述了用于检测病原体感染存在的方法。具体而言，该文件提供了一种检测从受试者获得的生物样品中的靶核酸如病原体特异性RNA的方法，其中该方法包括使用一个或多个脚趾开关传感器和等温扩增步骤来检测靶核酸。还提供了专门用于检测和鉴定诸如寨卡病毒等病毒存在的方法。

8. 发明申请

WO2021252956A2 RAPID LOW-COST DETECTION OF SARS-COV-2 USING ISOTHERMAL AMPLIFICATION AND SENSING METHODS 审中-公开
公开(公告)号：WO2021252956A2
公开(公告)日：2021-12-16
申请号：PCT/US2021/037092
申请日：2021-06-11
申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY , THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO
发明人： GREEN, Alexander , PARDEE, Keith , KARLIKOW, Margot , WU, Kaiyue , NOROUZI, Masoud
IPC分类号： C12N15/10 , C12Q1/68 , C12Q1/6816 , C12Q1/70
摘要： Provided herein are methods and compositions for rapid, highly sensitive detection of SARS-CoV-2 in biological samples. In particular, provided herein is a rapid, low-cost method for detecting SARS-CoV-2 that provides reliable, visible test results and does not require PCR reagents, elaborate biosafety precautions, or sophisticated laboratory equipment.

9. 发明申请

WO2017083693A1 METHOD OF PREPARING METAL DIBORIDE DISPERSIONS AND FILMS 审中-公开
标题翻译：制备金属二硼化物分散体和膜的方法
公开(公告)号：WO2017083693A1
公开(公告)日：2017-05-18
申请号：PCT/US2016/061594
申请日：2016-11-11
申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
发明人： GREEN, Alexander , YOUSAF, Ahmed , DEBNATH, Abishek
IPC分类号： C01B35/04 , C08J5/18 , C08J3/20 , C08K3/38 , C08L31/04
CPC分类号： C01B35/04 , C08J3/212 , C08J2329/04 , C08K3/38
摘要： Some embodiments include a method of producing metal diboride nanomaterials having thickness down to the atomic scale and lateral areas from 10 nm to over 1 μm by preparing a mixture of a metal diboride and a suspending solution. The suspending solution can be an organic solvent or a solution containing water, and optionally can include a dispersion agent, such as a surfactant, a polymer, small molecule, or biopolymer. Further, the method includes exfoliating the metal diboride by exposing the mixture to ultrasonic energy, centrifuging the mixture forming supernatant that includes a dispersion of exfoliated metal diborides, and extracting the dispersion from the supernatant. Some embodiments include extracting the supernatant and casting the solution by diluting the dispersion with a second suspending solution that includes dissolved polymer. This can result in a composite film includes a dispersion of the exfoliated metal diborides and provides improved mechanical properties.
摘要翻译：一些实施方案包括通过制备金属二硼化物和悬浮溶液的混合物来制备厚度低至原子尺度和横向面积为10nm至超过1μm的金属二硼化物纳米材料的方法。悬浮溶液可以是有机溶剂或含有水的溶液，并且任选地可以包括分散剂，例如表面活性剂，聚合物，小分子或生物聚合物。此外，所述方法包括通过将混合物暴露于超声波能量来剥离金属二硼化物，离心形成包含剥离的金属二硼化物分散体的上清液的混合物，并且从上清液中萃取分散体。一些实施方案包括提取上清液并通过用包含溶解的聚合物的第二悬浮溶液稀释分散液来流延溶液。这可导致复合膜包含剥离的金属二硼化物的分散体并提供改进的机械性能。

10. 发明申请

WO2022046979A1 RAPID AND LOW-COST NUCLEIC ACID DETECTION USING TRANSLATION-BASED SEQUENCE VERIFICATION ASSAYS 审中-公开
公开(公告)号：WO2022046979A1
公开(公告)日：2022-03-03
申请号：PCT/US2021/047676
申请日：2021-08-26
申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
发明人： GREEN, Alexander , WU, Kaiyue
IPC分类号： C12N15/85 , C12N15/09 , C12P19/34
摘要： Provided herein are methods and compositions for rapid, highly sensitive detection of a target nucleic acid in a sample, which may be indicative of the presence of a pathogen, disease condition, or disease predisposition. In particular, provided herein is a low-cost, portable method for detecting target nucleic acid sequences that rapidly provides reliable, visible test results and does not require elaborate biosafety precautions or sophisticated laboratory equipment.

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