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1. 发明申请

WO2003015901A1 TANDEM ISOTACHOPHORESIS/ZONE ELECTROPHORESIS METHOD AND SYSTEM 审中-公开
标题翻译： TANDEM ISOTACHOPHORESIS / ZONE ELECTROPHORESIS方法和系统
公开(公告)号：WO2003015901A1
公开(公告)日：2003-02-27
申请号：PCT/US2002/026393
申请日：2002-08-19
申请人： ACLARA BIOSCIENCES, INC. , WILLIAMS, Stephen, J. , TAN, Hong, Dong , KAO, Hung, Pin , VREELAND, Wyatt, N.
发明人： WILLIAMS, Stephen, J. , TAN, Hong, Dong , KAO, Hung, Pin , VREELAND, Wyatt, N.
IPC分类号： B01D57/02
CPC分类号： B01L3/5027 , B01L3/502753 , B01L2200/0605 , B01L2300/0816 , B01L2400/0415 , B01L2400/0421 , B01L2400/049 , C07K1/26 , C07K1/28 , G01N27/44773
摘要： A method of separating components having a given negative or positive charge and contained in a sample is disclosed. The method involves, in one embodiment, loading a microchannel with a sample, placed between a trailing- edge electrolyte having a selected concentration of a titratable species, and a leading-edge electrolyte. With the application of a voltage potential across the microchannel, charged components in the sample stack by isotachophoresis, and electrolytic hydroxyl or hydrogen ions formed by electrolysis at the upstream-end electrode migrate into the trailing-edge ion buffer, titrating the titratable species therein, where the concentration of the titratable species in the trailing-edge electrolyte is selected, in relation to the lengths of the upstream channel region and sample-loading volume, to permit the sample to stack into a relatively small sample volume before electrolytic-ion migration from the upstream electrode into and through the sample-volume region is effective to overtake the charged sample components. With continued application of an electric potential across the channel ends, charged sample components in the stacked sample volume separate by zone electrophoresis.
摘要翻译：公开了一种分离具有给定负电荷并包含在样品中的组分的方法。在一个实施方案中，该方法涉及将具有样品的微通道加载在具有选定浓度的可滴定物质的后缘电解质和前沿电解质之间。通过在微通道上施加电压电位，通过等速电泳施加样品叠层中的带电组分，并且通过在上游端电极处电解形成的电解羟基或氢离子迁移到后缘离子缓冲液中，滴定其中的可滴定物质，其中选择后缘电解质中的可滴定物质的浓度，相对于上游通道区域的长度和样品加载体积，以允许样品在电离离子迁移之前堆积成相对小的样品体积进入和通过样品体积区域的上游电极有效地超过带电的样品组分。通过在通道末端继续施加电位，通过区域电泳分离堆叠样品体积中的带电样品组分。

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