专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO0024929A2 LINEAR AMPLIFICATION MEDIATED PCR (LAM PCR) 审中-公开
标题翻译：关于线性扩增介导PCR（= LAM PCR）
公开(公告)号：WO0024929A2
公开(公告)日：2000-05-04
申请号：PCT/EP9908077
申请日：1999-10-26
申请人： VON KALLE CHRISTOF , SCHMIDT MANFRED
发明人： VON KALLE CHRISTOF , SCHMIDT MANFRED
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12Q2563/131 , C12Q2531/101 , C12Q2521/301
摘要： The invention relates to a highly sensitive method for identifying and/or sequencing an unknown DNA or RNA sequence which borders on a known DNA or RNA region. Said method is characterized by the following steps: (a) subjecting in a first step one or more DNA or RNA fragments to one or more linear PCR steps using one or more primers, (b) complementing the obtained single strands to give double strands, (c) digesting the double strands with one or more restriction enzymes to generate smooth and/or cohesive ends, (d) adding an oligonucleotide of known sequence to the digested ends and (e) propagating and detecting the DNA fragments thus obtained.
摘要翻译：描述了一种用于鉴定和/或测序邻近已知DNA或RNA区域的未知DNA或RNA序列的高度灵敏的方法，其包括（a）第一步中的一种或多种DNA或RNA （B）将所得单链填入双链（c）用一种或多种限制酶切割双链以产生光滑和/或粘性末端（d）切割末端连接已知序列的寡核苷酸并（e）繁殖并检测获得的DNA片段。

2. 发明申请

WO2008071682A8 NON-RANDOMNESS IN THE CLUSTERING OR GENOMIC EVENTS DETECTS INSERTIONAL MUTAGENESIS IN CLINICAL GENE THERAPY 审中-公开
标题翻译：聚集或遗传事件中的非随机性检测临床基因治疗中的插入性突变
公开(公告)号：WO2008071682A8
公开(公告)日：2008-12-24
申请号：PCT/EP2007063666
申请日：2007-12-11
申请人： DKFZ KREBSFORSCHUNGSZENTRUM , UNIV RUPRECHT KARLS HEIDELBERG , ABEL ULRICH , VON KALLE CHRISTOF , SCHMIDT MANFRED
发明人： VON KALLE CHRISTOF , SCHMIDT MANFRED
IPC分类号： G06F19/24
CPC分类号： G06F19/24

3. 发明申请

WO2008071682A2 NON-RANDOMNESS IN THE CLUSTERING OR GENOMIC EVENTS DETECTS INSERTIONAL MUTAGENESIS IN CLINICAL GENE THERAPY 审中-公开
标题翻译：聚集或遗传事件中的非随机性检测临床基因治疗中的插入性突变
公开(公告)号：WO2008071682A2
公开(公告)日：2008-06-19
申请号：PCT/EP2007/063666
申请日：2007-12-11
申请人： DKFZ DEUTSCHES KREBSFORSCHUNGSZENTRUM , RUPRECHT-KARLS-UNIVERSITÄT HEIDELBERG , ABEL, Ulrich , VON KALLE, Christof , SCHMIDT, Manfred
发明人： VON KALLE, Christof , SCHMIDT, Manfred
IPC分类号： G01N33/50
CPC分类号： G06F19/24
摘要： Features such as mutations or structural characteristics can be non-randomly distributed within a genomic DNA. As an example, insertional mutagenesis is of great importance in cancer research, preclinical and clinical retroviral vector gene therapy. Even when detectable side effects of vector integration are absent,the presence of insertions at higher than expected frequency at particular loci, termed common integration sites (CIS), is indicative of a biological of the integration at that site, and indicates a selective advantage of the affected cell clones based on their integration site (IS). So far, only computational simulations could assess whether the distribution of IS or other DNA features was significantly different compared to random. These simulations require extensive computational resources and fixed parameters of CIS definition. Here, we show that statistical inferences on the distribution of IS are possible using mathematical formulae to calculate the expected frequency of insertions based on genome size, number of sampled integration sites and CIS window size. P-values can be calculated based on the Poisson-distribution. Thus, for each individual problem a flexible CIS definition can be applied to most effectively identify non-random IS distribution mathematically. We here show that application of these calculations on clinical trials detect subtle, but substantial biological effects of insertion sites on clonal selection of hematopoiesis in humans.
摘要翻译：特征如突变或结构特征可以非随机分布在基因组DNA内。作为一个例子，插入突变在癌症研究，临床前和临床逆转录病毒载体基因治疗中是非常重要的。即使不存在可检测到的载体整合的副作用，在特定位点（称为共同整合位点（CIS））高于预期频率的插入的存在表明该位点整合的生物学，并且表明选择性优势受影响的细胞克隆基于其整合位点（IS）。到目前为止，只有计算模拟可以评估IS或其他DNA特征的分布是否与随机显着不同。这些模拟需要广泛的计算资源和CIS定义的固定参数。在这里，我们显示，使用数学公式可以使用基于基因组大小，抽样积分位点数和CIS窗口大小来计算插入的预期频率的IS分布统计学推断。 P值可以基于泊松分布计算。因此，对于每个单独的问题，可以应用灵活的CIS定义以最有效地以数学方式识别非随机IS分布。我们在这里表明，这些计算在临床试验中的应用检测到微小的，但是插入位点的重大生物效应对人造血细胞克隆选择的影响。

4. 发明申请

WO2007008309A2 STEM AND PROGENITOR CELL EXPANSION BY EVI, EVI-LIKE GENES AND SETBP1 审中-公开
标题翻译： EVI，EVI-LIKE基因和SETBP1的干细胞和前体细胞扩增
公开(公告)号：WO2007008309A2
公开(公告)日：2007-01-18
申请号：PCT/US2006/021413
申请日：2006-06-01
申请人： CHILDREN'S HOSPITAL MEDICAL CENTER , VON KALLE, Christof , SCHMIDT, Manfred , GREZ, Manuel
发明人： VON KALLE, Christof , SCHMIDT, Manfred , GREZ, Manuel
IPC分类号： C12N15/10 , C12N5/10 , C12N15/861
CPC分类号： C12N5/0634 , A61K48/00 , C12N2501/998 , C12N2510/00 , C12N2799/027
摘要： A method of increasing cell proliferation by modulating levels of EVI and related genes. Activation of EVI-1, PRDM16, or SETBP1 can increase the proliferation rate, self renewal and/or in vitro and/or in vivo survival and/or engraftment of human cells, either in vitro or in vivo. The gene modulation can be performed by various means, including traditional cloning methods and retroviral-based gene activation methods. The method can also be used to more efficiently deliver gene-corrected cells to a patient in need of treatment.
摘要翻译：通过调节EVI和相关基因水平来增加细胞增殖的方法。 EVI-1，PRDM16或SETBP1的活化可以在体外或体内增加人细胞的增殖速率，自我更新和/或体外和/或体内存活和/或植入。基因调控可以通过各种手段进行，包括传统的克隆方法和基于逆转录病毒的基因激活方法。该方法还可用于更有效地将基因校正的细胞递送至需要治疗的患者。

5. 发明申请

WO2011086118A1 IN VIVO DETERMINATION OF THE LOKALIZATION OF DNA DOUBLE-STAND BREAKS AND APPLICATION THEREOF 审中-公开
标题翻译： DNA双链断裂及其应用的LOKAL化的体外测定
公开(公告)号：WO2011086118A1
公开(公告)日：2011-07-21
申请号：PCT/EP2011/050380
申请日：2011-01-13
申请人： DEUTSCHES KREBSFORSCHUNGSZENTRUM , FONDAZIONE CENTRO SAN RAFFAELE DEL MONTE TABOR , VON KALLE, Christof , SCHMIDT, Manfred , GABRIEL, Richard , NOWROUZI, Ali , ARENS, Anne , LOMBARDO, Angelo , NALDINI, Luigi
发明人： VON KALLE, Christof , SCHMIDT, Manfred , GABRIEL, Richard , NOWROUZI, Ali , ARENS, Anne , LOMBARDO, Angelo , NALDINI, Luigi
IPC分类号： C12Q1/68 , C12N15/90
CPC分类号： C12Q1/686 , C12N15/907 , C12N2740/15021 , C12N2740/15031 , C12N2740/15045 , C12Q1/6827 , C12Q1/6858 , C12Q1/70 , C12Q2521/301
摘要： The present invention relates to a method for determining the in vivo localization of double-strand breaks in a host cell, comprising incubating a host cell suspected to comprise DNA double-strand breaks and a linear polynucleotide comprising a known sequence, detecting the in vivo insertion sites of said polynucleotide in the genome of said host cell, and assessing the in vivo localization of double-strand breaks. Further envisaged by the present invention is a method for obtaining an endonuclease with altered in vivo specificity. Finally, the present invention is directed to a kit for determining in vivo specificity of an endonuclease.
摘要翻译：本发明涉及用于确定宿主细胞中双链断裂的体内定位的方法，包括孵育疑似包含DNA双链断裂的宿主细胞和包含已知序列的线性多核苷酸，检测体内插入所述多核苷酸在所述宿主细胞的基因组中的位点，以及评估双链断裂的体内定位。本发明进一步设想的是获得具有改变的体内特异性的核酸内切酶的方法。最后，本发明涉及用于确定内切核酸酶的体内特异性的试剂盒。

6. 发明申请

WO2007008309A3 STEM AND PROGENITOR CELL EXPANSION BY EVI, EVI-LIKE GENES AND SETBP1 审中-公开
标题翻译：由EVI，EVI样基因和SETBP1进行干细胞和前列腺细胞扩增
公开(公告)号：WO2007008309A3
公开(公告)日：2007-07-26
申请号：PCT/US2006021413
申请日：2006-06-01
申请人： CHILDRENS HOSP MEDICAL CENTER , VON KALLE CHRISTOF , SCHMIDT MANFRED , GREZ MANUEL
发明人： VON KALLE CHRISTOF , SCHMIDT MANFRED , GREZ MANUEL
IPC分类号： C12N15/10 , C12N5/10 , C12N15/861
CPC分类号： C12N5/0634 , A61K48/00 , C12N2501/998 , C12N2510/00 , C12N2799/027
摘要： A method of increasing cell proliferation by modulating levels of EVI and related genes. Activation of EVI-1, PRDM16, or SETBP1 can increase the proliferation rate, self renewal and/or in vitro and/or in vivo survival and/or engraftment of human cells, either in vitro or in vivo. The gene modulation can be performed by various means, including traditional cloning methods and retroviral-based gene activation methods. The method can also be used to more efficiently deliver gene-corrected cells to a patient in need of treatment.
摘要翻译：通过调节EVI和相关基因水平来增加细胞增殖的方法。 EVI-1，PRDM16或SETBP1的激活可以在体外或体内增加人细胞的增殖速率，自我更新和/或体外和/或体内存活和/或植入。基因调节可以通过各种方式进行，包括传统的克隆方法和基于逆转录病毒的基因活化方法。该方法还可以用于更有效地将基因校正的细胞递送至需要治疗的患者。

7. 发明申请

WO0024929A9 LINEAR AMPLIFICATION MEDIATED PCR (LAM PCR) 审中-公开
标题翻译：关于线性扩增介导的PCR（PCR = LAM）
公开(公告)号：WO0024929A9
公开(公告)日：2001-07-12
申请号：PCT/EP9908077
申请日：1999-10-26
申请人： VON KALLE CHRISTOF , SCHMIDT MANFRED
发明人： VON KALLE CHRISTOF , SCHMIDT MANFRED
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12Q2563/131 , C12Q2531/101 , C12Q2521/301
摘要： The invention relates to a highly sensitive method for identifying and/or sequencing an unknown DNA or RNA sequence which borders on a known DNA or RNA region. Said method is characterized by the following steps: (a) subjecting in a first step one or more DNA or RNA fragments to one or more linear PCR steps using one or more primers, (b) complementing the obtained single strands to give double strands, (c) digesting the double strands with one or more restriction enzymes to generate smooth and/or cohesive ends, (d) adding an oligonucleotide of known sequence to the digested ends and (e) propagating and detecting the DNA fragments thus obtained.
摘要翻译：它是描述一个未知DNA或RNA序列的鉴定和/或测序的高灵敏度方法，相邻于一个已知的DNA或RNA区，其中（a）在第一阶段中的一个或多个DNA或RNA 的使用一个或多个引物的一个或多个线性PCR步骤片段受试者在双链中获得的单链（b）中填充（c）与一种或多种限制的双链酶切割，以产生平滑的和/或粘性末端（D）的切割端附着已知序列和（e）中传播的所得的DNA片段，并检测的寡核苷酸。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式