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    • 3. 发明申请
    • PROCESS FOR THE ENZYMATIC FORMATION OF AMIDE BONDS
    • 酰胺凝胶的形成过程
    • WO2014067746A1
    • 2014-05-08
    • PCT/EP2013/070714
    • 2013-10-04
    • EVONIK INDUSTRIES AGOSSWALD, SteffenBINDL, Martin
    • OSSWALD, SteffenBINDL, Martin
    • C12P13/02C12P13/04
    • C12P13/02C12P13/04
    • The present invention is concerned with processes for the enzymatic formation of amide bonds. More specifically the invention is concerned with the formation of amide bonds between amino acid esters acylated on the amino terminus to nicotinic acid or isonicotinic acid and amino compounds with the aid of proteases. The invention provides processes for amide formation employing amino acid esters, connected to nicotinoyl- or isonicotinoyl-groups via an amide bond on the amino terminus, that are more efficient than processes for amide formation employing amino acid esters carrying standard protecting groups such as benzoyl or Z on their amino terminus. In one aspect therefore, the present invention, further, relates to the protease mediated synthesis of organogellant compounds (OG) as depicted below wherein L is a linking moiety of molecular weight from 14 g/mol to 500 g/mol, one of X 1 , X 2 is nitrogen and the other is carbon, and wherein R 1 are sidechain substituents.
    • 本发明涉及酶形成酰胺键的方法。 更具体地,本发明涉及在氨基末端酰化于烟酸或异烟酸和氨基化合物之间借助蛋白酶形成氨基酸酯之间的酰胺键。 本发明提供使用氨基酸酯,通过氨基末端上的酰胺键连接到烟酰基或异烟酰基的酰胺形成方法,其比使用具有标准保护基团的氨基酸酯(例如苯甲酰基或 Z在其氨基末端。 因此,一方面,本发明还涉及如下所述的有机凝胶化合物(OG)的蛋白酶介导的合成,其中L是分子量为14g / mol至500g / mol的连接部分,X1, X2是氮,另一个是碳,并且其中R1是侧链取代基。