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1. 发明申请

WO2011031438A3 PORCINE TORQUE TENO VIRUS VACCINES AND DIAGNOSIS 审中-公开
标题翻译：猪瘟TENO病毒疫苗及其诊断
公开(公告)号：WO2011031438A3
公开(公告)日：2011-06-30
申请号：PCT/US2010046330
申请日：2010-08-23
申请人： VIRGINIA TECH INTELL PROP , MENG XIANG-JIN , HUANG YAOWEI
发明人： MENG XIANG-JIN , HUANG YAOWEI
IPC分类号： C12N15/34 , A01K67/027 , A61K39/12 , C12N15/63 , C12Q1/68 , G01N33/569
CPC分类号： G01N33/56983 , A61K39/12 , A61K2039/552 , C07K14/005 , C07K16/081 , C12N7/00 , C12N2750/00021 , C12N2750/00022 , C12N2750/00034 , C12N2750/14034 , C12Q1/701 , G01N2333/01 , G01N2333/085 , G01N2469/20
摘要： The present invention provides four purified preparation containing a polynucleic acid molecule encoding porcine Torque teno virus (PTTV) genotypes or subtypes PTTV1a-VA, PTTV1b-VA, PTTV2b-VA, and PTTV2c-VA. The present invention also provides infectious DNA clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of the same. The present invention further provides live, attenuated, vector-expressed and purified recombinant capsid subunit or killed viral vaccines for protection against PTTV infection. The present invention additionally provides subunit vaccines comprising PTTV specific gene products, especially ORF1 capsid gene product for protection against PTTV infection. Further, the present invention provides methods for diagnosing PTTV infection via polymerase chain reaction (PCR) using specific primer for PTTV1, PTTV2, and individual PTTV1 genotypes. Finally, the present invention provides methods for diagnosing PTTV infection via immunological methods, e.g., enzyme-linked immunoabsorbent assay (ELISA) and Western blot using PTTV specific antigens for detecting serum PTTV specific antibodies.
摘要翻译：本发明提供了四种含有编码猪扭矩型诺沃病毒（PTTV）基因型或亚型PTTV1a-VA，PTTV1b-VA，PTTV2b-VA和PTTV2c-VA的多核酸分子的纯化制剂。本发明还提供了感染性DNA克隆，生物功能性质粒或含有其的感染性核酸基因组分子的病毒载体。本发明进一步提供活的，减毒的，载体表达和纯化的重组衣壳亚单位或杀死的病毒疫苗以防止PTTV感染。本发明另外提供了包含PTTV特异性基因产物的亚单位疫苗，特别是用于防止PTTV感染的ORF1衣壳基因产物。此外，本发明提供了使用用于PTTV1，PTTV2和个体PTTV1基因型的特异性引物通过聚合酶链式反应（PCR）诊断PTTV感染的方法。最后，本发明提供了通过免疫学方法诊断PTTV感染的方法，例如酶联免疫吸附测定（ELISA）和使用PTTV特异性抗原的Western印迹来检测血清PTTV特异性抗体。

2. 发明申请

WO2011031438A9 PORCINE TORQUE TENO VIRUS VACCINES AND DIAGNOSIS 审中-公开
标题翻译：猪瘟TENO病毒疫苗及其诊断
公开(公告)号：WO2011031438A9
公开(公告)日：2011-03-17
申请号：PCT/US2010/046330
申请日：2010-08-23
申请人： VIRGINIA TECH INTELLECTUAL PROPERTIES, INC. , MENG, Xiang-Jin , HUANG, Yaowei
发明人： MENG, Xiang-Jin , HUANG, Yaowei
IPC分类号： C12N15/34 , C12N15/63 , A61K39/12 , A01K67/027 , C12Q1/68 , G01N33/569
摘要： The present invention provides four purified preparation containing a polynucleic acid molecule encoding porcine Torque teno virus (PTTV) genotypes or subtypes PTTV1a-VA, PTTV1b-VA, PTTV2b-VA, and PTTV2c-VA. The present invention also provides infectious DNA clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of the same. The present invention further provides live, attenuated, vector-expressed and purified recombinant capsid subunit or killed viral vaccines for protection against PTTV infection. The present invention additionally provides subunit vaccines comprising PTTV specific gene products, especially ORF1 capsid gene product for protection against PTTV infection. Further, the present invention provides methods for diagnosing PTTV infection via polymerase chain reaction (PCR) using specific primer for PTTV1, PTTV2, and individual PTTV1 genotypes. Finally, the present invention provides methods for diagnosing PTTV infection via immunological methods, e.g., enzyme-linked immunoabsorbent assay (ELISA) and Western blot using PTTV specific antigens for detecting serum PTTV specific antibodies.
摘要翻译：本发明提供了含有编码猪扭矩型诺沃病毒（PTTV）基因型或亚型PTTV1a-VA，PTTV1b-VA，PTTV2b-VA和PTTV2c-VA的多核酸分子的四种纯化制剂。本发明还提供了感染性DNA克隆，生物功能性质粒或含有其的感染性核酸基因组分子的病毒载体。本发明进一步提供活的，减毒的，载体表达和纯化的重组衣壳亚单位或杀死的病毒疫苗以防止PTTV感染。本发明另外提供了包含PTTV特异性基因产物的亚单位疫苗，特别是用于防止PTTV感染的ORF1衣壳基因产物。此外，本发明提供了使用用于PTTV1，PTTV2和个体PTTV1基因型的特异性引物通过聚合酶链式反应（PCR）诊断PTTV感染的方法。最后，本发明提供了用于通过免疫学方法诊断PTTV感染的方法，例如酶联免疫吸附测定（ELISA）和使用PTTV特异性抗原的Western印迹来检测血清PTTV特异性抗体。

3. 发明申请

WO2009128878A1 CHIMERIC PORCINE CIRCOVIRUS PCV2Gen-1Rep AND USES THEREOF 审中-公开
标题翻译： CHIMERIC PORCINE CIRCOVIRUS PCV2Gen-1Rep及其用途
公开(公告)号：WO2009128878A1
公开(公告)日：2009-10-22
申请号：PCT/US2009/002189
申请日：2009-04-08
申请人： VIRGINIA TECH INTELLECTUAL PROPERTIES, INC. , JUHAN, Nicole, M. , MENG, Xiang-jin
发明人： JUHAN, Nicole, M. , MENG, Xiang-jin
IPC分类号： A61K39/12
CPC分类号： C12N7/00 , A61K2039/525 , C12N2750/10021
摘要： The present invention relates to a novel chimeric nucleic acid molecule of porcine circovirus (PCV2Gen-lRep) that embraces a nucleic acid molecule encoding porcine circovirus type 2 (PCV2) which contains a nucleic acid sequence encoding a Rep protein of porcine circovirus type 1 (PCVl), particularly wherein the nucleic acid sequence encoding the Rep protein of PCVl is an open reading frame (ORF) gene and, more particularly, wherein the ORF Rep gene is ORFl. A highly desirable chimeric nucleic acid molecule is constructed by replacing the ORFl Rep gene of PCV2 by the ORFl Rep gene of PCVl. The invention also encompasses the biologically functional plasmid or viral vector containing the unique chimeric nucleic acid molecules, suitable host cells transfected by the plasmid or vector, infectious chimeric porcine circoviruses that are produced by the suitable host cells, the process for the production of an immunogenic polypeptide product making use of the new chimera, viral vaccines that protect a pig against viral infection or postweaning multisystemic wasting syndrome (PMWS) caused by PCV2, methods of protecting a pig against viral infection or postweaning multisystemic wasting syndrome (PMWS) caused by PCV2, methods of preparing the unique chimera of PCV2Gen-lRep and the like. This invention further includes a new method for improving the replication and titer of PCV2 in a cell culture.
摘要翻译：本发明涉及一种包含编码猪圆环病毒2型（PCV2）的核酸分子的猪圆环病毒嵌合核酸分子（PCV2Gen-1Rep），其包含编码猪圆环病毒1型Rep蛋白（PCV1）的核酸序列），特别是其中编码PCV1的Rep蛋白的核酸序列是开放阅读框（ORF）基因，更具体地，其中ORF Rep基因是ORF1。通过用PCV1的ORF1 Rep基因替换PCV2的ORF1 Rep基因来构建非常需要的嵌合核酸分子。本发明还包括含有独特的嵌合核酸分子，由质粒或载体转染的合适的宿主细胞的生物功能质粒或病毒载体，由合适的宿主细胞产生的感染性嵌合猪圆环病毒，产生免疫原性的方法使用新的嵌合体的多肽产物，保护猪免受PCV2引起的病毒感染或断奶后多系统消耗综合征（PMWS）的病毒疫苗，保护猪免受病毒感染或由PCV2引起的断奶后多系统消耗综合征（PMWS）的方法，制备PCV2Gen-lRep等独特嵌合体的方法。本发明还包括用于改善细胞培养物中PCV2的复制和滴度的新方法。

4. 发明申请

WO03063679A8 HEPATITIS E VIRUS CLONES AND METHODS OF THEIR USE 审中-公开
标题翻译： HEPATITIS E病毒克隆及其使用方法
公开(公告)号：WO03063679A8
公开(公告)日：2004-12-23
申请号：PCT/US0236096
申请日：2002-11-08
申请人： US GOV HEALTH & HUMAN SERV , EMERSON SUZANNE U , PURCELL ROBERT H , ZHANG MINGDONG , MENG XIANG-JIN
发明人： EMERSON SUZANNE U , PURCELL ROBERT H , ZHANG MINGDONG , MENG XIANG-JIN
IPC分类号： C07K14/08 , C12N7/00 , C12N7/04 , C12N15/51 , G01N33/576 , C07H21/04 , A61K31/711 , A61K39/29
CPC分类号： G01N33/5767 , A61K2039/5254 , C07K14/005 , C12N7/00 , C12N2770/28122 , C12N2770/28161 , G01N2500/10
摘要： Full-length cDNA clones of the Sar-55 strain of HEV that are infectious in primates are disclosed. The invention relates to HEV nucleic acid sequences, their encoded proteins, any variants thereof, and HEV antibodies for developing vaccines of HEV, and detecting, preventing, and/or treating hepatitis E in a mammal. In particular, the invention provides nucleic acid sequences comprising infectious hepatitis E viruses of the Pakastani (Sar-55) strain. The invention therefore relates to the use of these sequences, and polypeptides encoded by all or part of these sequences, in the development of vaccines and diagnostic assays for HEV and in the development of screening assays for the identification of antiviral agents for HEV.
摘要翻译：公开了在灵长类动物感染的HEV的Sar-55菌株的全长cDNA克隆。本发明涉及HEV核酸序列，其编码的蛋白质，其任何变体和HEV抗体，用于开发HEV疫苗，以及检测，预防和/或治疗哺乳动物中的戊型肝炎。特别地，本发明提供了包含Pakastani（Sar-55）菌株的感染性戊型肝炎病毒的核酸序列。因此，本发明涉及这些序列和由这些序列的全部或部分编码的多肽在开发疫苗和用于HEV的诊断测定中的用途，以及用于鉴定HEV抗病毒剂的筛选测定的开发。

5. 发明申请

WO03063679A2 HEPATITIS E VIRUS CLONES AND METHODS OF THEIR USE 审中-公开
标题翻译： HEPATITIS E病毒克隆及其使用方法
公开(公告)号：WO03063679A2
公开(公告)日：2003-08-07
申请号：PCT/US0236096
申请日：2002-11-08
申请人： US GOV HEALTH & HUMAN SERV , EMERSON SUZANNE U , PURCELL ROBERT H , ZHANG MINGDONG , MENG XIANG-JIN
发明人： EMERSON SUZANNE U , PURCELL ROBERT H , ZHANG MINGDONG , MENG XIANG-JIN
IPC分类号： C07K14/08 , C12N7/00 , C12N7/04 , C12N15/51 , G01N33/576 , A61B
CPC分类号： G01N33/5767 , A61K2039/5254 , C07K14/005 , C12N7/00 , C12N2770/28122 , C12N2770/28161 , G01N2500/10
摘要： Full-length cDNA clones of the Sar-55 strain of HEV that are infectious in primates are disclosed. The invention relates to HEV nucleic acid sequences, their encoded proteins, any variants thereof, and HEV antibodies for developing vaccines of HEV, and detecting, preventing, and/or treating hepatitis E in a mammal. In particular, the invention provides nucleic acid sequences comprising infectious hepatitis E viruses of the Pakastani (Sar-55) strain. The invention therefore relates to the use of these sequences, and polypeptides encoded by all or part of these sequences, in the development of vaccines and diagnostic assays for HEV and in the development of screening assays for the identification of antiviral agents for HEV.
摘要翻译：公开了在灵长类动物中感染的HEV Sar-55株的全长cDNA克隆。本发明涉及用于开发HEV疫苗以及检测，预防和/或治疗哺乳动物戊型肝炎的HEV核酸序列，其编码蛋白，其任何变体和HEV抗体。特别地，本发明提供了包含Pakastani（Sar-55）菌株的传染性戊型肝炎病毒的核酸序列。因此，本发明涉及这些序列和由全部或部分这些序列编码的多肽在HEV的疫苗和诊断测定法的开发以及用于鉴定抗病毒剂用于HEV的筛选测定的开发中的用途。

6. 发明申请

WO2002089733A2 RECOMBINANT ORF2 PROTEINS OF THE SWINE HEPATITIS E VIRUS AND THEIR USE AS A VACCINE AND AS A DIAGNOSTIC REAGENT FOR MEDICAL AND VETERINARY APPLICATIONS 审中-公开
标题翻译：西班牙乙型肝炎病毒的重组ORF2蛋白及其用作疫苗和用作医学和兽医应用的诊断试剂
公开(公告)号：WO2002089733A2
公开(公告)日：2002-11-14
申请号：PCT/US2002/014100
申请日：2002-05-02
申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES , MENG, Xiang-Jin , PURCELL, Robert, H. , EMERSON, Suzanne, U.
发明人： MENG, Xiang-Jin , PURCELL, Robert, H. , EMERSON, Suzanne, U.
IPC分类号： A61K
CPC分类号： C07K14/005 , A61K38/00 , A61K39/00 , A61K2039/505 , C12N2710/14143 , C12N2770/28122 , C12N2770/28134 , G01N33/5767 , G01N2800/52
摘要： The invention relates to open reading frame 2 (ORF-2) proteins of a swine hepatitis E virus and the use of these proteins as an antigen in diagnostic immunoassays and/or as immunogen or vaccine to protect against infection by hepatitis E.
摘要翻译：本发明涉及猪戊型肝炎病毒的开放阅读框架2（ORF-2）蛋白质和这些蛋白质作为诊断免疫测定中的抗原的用途和/或作为免疫原或疫苗以防止乙型肝炎感染。

7. 发明申请

WO2009058285A2 PORCINE DC-SIGN, ICAM-3 AND LSECtin AND USES THEREOF 审中-公开
标题翻译： PORCINE DC-SIGN，ICAM-3和LSECtin及其用途
公开(公告)号：WO2009058285A2
公开(公告)日：2009-05-07
申请号：PCT/US2008/012251
申请日：2008-10-29
申请人： VIRGINIA TECH INTELLECTUAL PROPERTIES, INC. , HUANG, Yaowei , MENG, Xiang-Jin
发明人： HUANG, Yaowei , MENG, Xiang-Jin
IPC分类号： C07K14/43
CPC分类号： C07K14/70525 , C07K14/7056
摘要： The present invention relates to the cloning, identification and characterization of the unique and entire genomic sequences encoding new porcine DC-SIGN and LSECtin proteins, including the novel nucleotide sequences of the full-length cDNA and genes of both pDC- SIGN gene and pLSECtin. Also provided are the nucleic acid molecules encoding newly discovered porcine ICAM-3 isoforms from porcine monocyte-derived dendritic cells and the use thereof. Specifically, the invention is drawn to an isolated nucleic acid molecule comprising a nucleotide sequence encoding one or more of porcine DC-SIGN, porcine ICAM- 3, porcine LSECtin, a complement of the nucleotide sequence or a functional, defined portion of the nucleotide sequence or a protein fusion product linked to a protein that may be of porcine or human origin. Methods for isolating and cloning the new porcine genes and for using the new nucleotide sequences in improved methods for propagating viruses, particularly enveloped viruses, are additionally described herein. The invention further includes new transfected cells or cell lines that can stably express the porcine proteins, new antibodies and the like.
摘要翻译：本发明涉及克隆，鉴定和表征编码新的猪DC-SIGN和LSECtin蛋白的独特和完整的基因组序列，包括全长cDNA的新型核苷酸序列和 pDC-SIGN基因和pLSECtin。还提供了编码来自猪单核细胞来源的树突细胞的新发现的猪ICAM-3同种型的核酸分子及其用途。具体而言，本发明涉及包含编码猪DC-SIGN，猪ICAM-3，猪LSECtin，核苷酸序列的互补序列或核苷酸序列的功能性限定部分中的一种或多种的核苷酸序列的分离的核酸分子或与可能是猪或人来源的蛋白质连接的蛋白质融合产物。本文额外描述了用于分离和克隆新猪基因的方法以及用于改进繁殖病毒特别是有包膜病毒的方法中的新核苷酸序列的方法。本发明还包括可稳定表达猪蛋白质的新转染细胞或细胞系，新抗体等。

8. 发明申请

WO2011153351A3 NOVEL MODIFIED LIVE-ATTENUATED VACCINES (MLV) AND SUBUNIT VACCINES CREATED BY DNA SHUFFLING AGAINST PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS (PRRSV) 审中-公开
标题翻译：新型改良的活体灭活疫苗（MLV）和亚硝酸盐灭活剂对磷酸盐生殖和呼吸道综合症病毒（PRRSV）
公开(公告)号：WO2011153351A3
公开(公告)日：2012-06-28
申请号：PCT/US2011038930
申请日：2011-06-02
申请人： VIRGINIA TECH INTELL PROP , NI YANYAN , HUANG YAO-WEI , MENG XIANG-JIN
发明人： NI YANYAN , HUANG YAO-WEI , MENG XIANG-JIN
IPC分类号： C12N15/40 , A61K39/12 , A61P31/14 , C12N15/62 , C12N15/63
CPC分类号： A61K39/12 , A61K2039/5254 , A61K2039/552 , C12N7/00 , C12N2770/10034 , C12N2770/10052
摘要： The present invention provides a novel infectious cDNA clone of porcine reproductive and respiratory syndrome virus (PRRSV), particularly for PRRSV strain VR2385; an improved DNA-launched reverse genetics system for PRRSV; infectious chimeric PRRSV viruses generated through DNA shuffling; modified live- attenuated virus vaccines (MLV) using DNA shuffled chimeric viruses; chimeric viral proteins produced through shuffled chimeric viruses; PRRSV antigens and subunit vaccines based on shuffled chimeric viral proteins; and method of producing broadly protective PRRSV vaccines using DNA shuffling techniques. Particularly, the present invention provides infectious chimeric viruses generated by DNA shuffling of the GP5 genes of genetically distinct strains of PRRSV; modified live-attenuated vaccines comprise infectious chimeric viruses containing the shuffled GP5 genes and/or other shuffled PRRSV proteins (such as GP2, GP3, GP4, M, and non- structural proteins); broadly -protective subunit vaccines comprising PRRSV chimeric shuffled GP5 protein and/or other shuffled PRRSV proteins (such as GP2, GP3, GP4, M, and non- structural proteins).
摘要翻译：本发明提供猪繁殖与呼吸综合征病毒（PRRSV）的新型感染性cDNA克隆，特别是PRRSV株VR2385的感染性cDNA克隆; 用于PRRSV的改进的DNA推出的反向遗传学系统; 通过DNA改组产生的感染性嵌合PRRSV病毒; 使用DNA洗牌嵌合病毒的改良活减毒病毒疫苗（MLV）; 通过混洗嵌合病毒产生的嵌合病毒蛋白; 基于混洗嵌合病毒蛋白的PRRSV抗原和亚单位疫苗; 以及使用DNA改组技术生产广泛保护性PRRSV疫苗的方法。特别地，本发明提供了通过遗传上不同的PRRSV菌株的GP5基因的DNA改组产生的感染性嵌合病毒; 修饰的活减毒疫苗包括含有洗牌的GP5基因和/或其它改组的PRRSV蛋白（例如GP2，GP3，GP4，M和非结构蛋白）的感染性嵌合病毒; 包括PRRSV嵌合洗牌的GP5蛋白和/或其它改组的PRRSV蛋白（例如GP2，GP3，GP4，M和非结构蛋白）的广泛保护性亚单位疫苗。

9. 发明申请

WO2011153351A2 NOVEL MODIFIED LIVE-ATTENUATED VACCINES (MLV) AND SUBUNIT VACCINES CREATED BY DNA SHUFFLING AGAINST PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS (PRRSV) 审中-公开
标题翻译：新型改良的活体灭活疫苗（MLV）和亚硝酸盐灭活剂对磷酸盐生殖和呼吸道综合症病毒（PRRSV）
公开(公告)号：WO2011153351A2
公开(公告)日：2011-12-08
申请号：PCT/US2011/038930
申请日：2011-06-02
申请人： VIRGINIA TECH INTELLECTUAL PROPERTIES, INC. , NI, Yanyan , HUANG, Yao-Wei , MENG, Xiang-Jin
发明人： NI, Yanyan , HUANG, Yao-Wei , MENG, Xiang-Jin
IPC分类号： C12N15/40 , C12N15/63 , C12N15/62 , A61K39/12 , A61P31/14
CPC分类号： A61K39/12 , A61K2039/5254 , A61K2039/552 , C12N7/00 , C12N2770/10034 , C12N2770/10052
摘要： The present invention provides a novel infectious cDNA clone of porcine reproductive and respiratory syndrome virus (PRRSV), particularly for PRRSV strain VR2385; an improved DNA-launched reverse genetics system for PRRSV; infectious chimeric PRRSV viruses generated through DNA shuffling; modified live- attenuated virus vaccines (MLV) using DNA shuffled chimeric viruses; chimeric viral proteins produced through shuffled chimeric viruses; PRRSV antigens and subunit vaccines based on shuffled chimeric viral proteins; and method of producing broadly protective PRRSV vaccines using DNA shuffling techniques. Particularly, the present invention provides infectious chimeric viruses generated by DNA shuffling of the GP5 genes of genetically distinct strains of PRRSV; modified live-attenuated vaccines comprise infectious chimeric viruses containing the shuffled GP5 genes and/or other shuffled PRRSV proteins (such as GP2, GP3, GP4, M, and non- structural proteins); broadly -protective subunit vaccines comprising PRRSV chimeric shuffled GP5 protein and/or other shuffled PRRSV proteins (such as GP2, GP3, GP4, M, and non- structural proteins).
摘要翻译：本发明提供猪繁殖与呼吸综合征病毒（PRRSV）的新型感染性cDNA克隆，特别是PRRSV株VR2385的感染性cDNA克隆; 用于PRRSV的改进的DNA推出的反向遗传学系统; 通过DNA改组产生的感染性嵌合PRRSV病毒; 使用DNA洗牌嵌合病毒的改良活减毒病毒疫苗（MLV）; 通过混洗嵌合病毒产生的嵌合病毒蛋白; 基于混洗嵌合病毒蛋白的PRRSV抗原和亚单位疫苗; 以及使用DNA改组技术生产广泛保护性PRRSV疫苗的方法。特别地，本发明提供了通过遗传上不同的PRRSV菌株的GP5基因的DNA改组产生的感染性嵌合病毒; 修饰的活减毒疫苗包括含有洗牌的GP5基因和/或其它改组的PRRSV蛋白（例如GP2，GP3，GP4，M和非结构蛋白）的感染性嵌合病毒; 包括PRRSV嵌合洗牌的GP5蛋白和/或其它改组的PRRSV蛋白（例如GP2，GP3，GP4，M和非结构蛋白）的广泛保护性亚单位疫苗。

10. 发明申请

WO2009058285A3 PORCINE DC-SIGN, ICAM-3 AND LSECtin AND USES THEREOF 审中-公开
标题翻译： PORCINE DC-SIGN，ICAM-3和LSECtin及其用途
公开(公告)号：WO2009058285A3
公开(公告)日：2009-12-10
申请号：PCT/US2008012251
申请日：2008-10-29
申请人： VIRGINIA TECH INTELL PROP , HUANG YAOWEI , MENG XIANG-JIN
发明人： HUANG YAOWEI , MENG XIANG-JIN
IPC分类号： C07K14/705
CPC分类号： C07K14/70525 , C07K14/7056
摘要： The present invention relates to the cloning, identification and characterization of the unique and entire genomic sequences encoding new porcine DC-SIGN and LSECtin proteins, including the novel nucleotide sequences of the full-length cDNA and genes of both pDC- SIGN gene and pLSECtin. Also provided are the nucleic acid molecules encoding newly discovered porcine ICAM-3 isoforms from porcine monocyte-derived dendritic cells and the use thereof. Specifically, the invention is drawn to an isolated nucleic acid molecule comprising a nucleotide sequence encoding one or more of porcine DC-SIGN, porcine ICAM- 3, porcine LSECtin, a complement of the nucleotide sequence or a functional, defined portion of the nucleotide sequence or a protein fusion product linked to a protein that may be of porcine or human origin. Methods for isolating and cloning the new porcine genes and for using the new nucleotide sequences in improved methods for propagating viruses, particularly enveloped viruses, are additionally described herein. The invention further includes new transfected cells or cell lines that can stably express the porcine proteins, new antibodies and the like.
摘要翻译：本发明涉及克隆，鉴定和表征编码新的猪DC-SIGN和LSECtin蛋白的独特和完整的基因组序列，包括全长cDNA的新型核苷酸序列以及pDC-SIGN基因和pLSECtin的基因。还提供了编码来自猪单核细胞来源的树突细胞的新发现的猪ICAM-3同种型的核酸分子及其用途。具体而言，本发明涉及包含编码猪DC-SIGN，猪ICAM-3，猪LSECtin，核苷酸序列的互补序列或核苷酸序列的功能性限定部分中的一种或多种的核苷酸序列的分离的核酸分子或与可能是猪或人来源的蛋白质连接的蛋白质融合产物。本文额外描述了用于分离和克隆新猪基因的方法以及用于改进繁殖病毒特别是有包膜病毒的方法中的新核苷酸序列的方法。本发明还包括可稳定表达猪蛋白质的新转染细胞或细胞系，新抗体等。

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