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1. 发明申请

WO2011098551A3 OPTIMIZED CELLULASE ENZYMES 审中-公开
标题翻译：优化的纤维素酶
公开(公告)号：WO2011098551A3
公开(公告)日：2012-05-31
申请号：PCT/EP2011052023
申请日：2011-02-11
申请人： SUED CHEMIE AG , KETTLING ULRICH , REISINGER CHRISTOPH , BRUECK THOMAS , KOLTERMANN ANDRE , GERLACH JOCHEN , UNTERSTRASSER ISABEL , ROECHER LUTZ , RARBACH MARKUS , CLAREN JOERG , KOHL ANDREAS , PIECK JAN CARSTEN , SCHLOSSER DOMINIK
发明人： KETTLING ULRICH , REISINGER CHRISTOPH , BRUECK THOMAS , KOLTERMANN ANDRE , GERLACH JOCHEN , UNTERSTRASSER ISABEL , ROECHER LUTZ , RARBACH MARKUS , CLAREN JOERG , KOHL ANDREAS , PIECK JAN CARSTEN , SCHLOSSER DOMINIK
IPC分类号： C12N9/42
CPC分类号： C12N9/2437 , C12N9/24 , C12Y302/01091
摘要： The invention discloses cellulase enzymes with optimized properties for processing of cellulose- and lignocellulose-containing substrates. In particular, cellobiohydrolase enzymes with preferred characteristics are disclosed. The present invention provides fusion, insertion, deletion and/or substitution variants of such enzymes. Enzyme variants have enhanced thermostability, proteolytic stability, specific activity and/or stability at extreme pH. Nucleic acid molecules encoding said enzymes, a composition comprising said enzymes, a method for preparation, and the use for cellulose processing and/or for the production of biofuels are disclosed.
摘要翻译：本发明公开了具有优化的用于加工含纤维素和木质素纤维素的底物的纤维素酶。特别地，公开了具有优选特性的纤维二糖水解酶。本发明提供了这种酶的融合，插入，缺失和/或取代变体。酶变体在极端pH下具有增强的热稳定性，蛋白水解稳定性，比活性和/或稳定性。公开了编码所述酶的核酸分子，包含所述酶的组合物，制备方法以及用于纤维素加工和/或生产生物燃料的用途。

2. 发明申请

WO2011080078A2 THERMOSTABLE XYLANASE FOR THE SELECTIVE HYDROLYSIS OF PENTOSE-CONTAINING POLYSACCHARIDES 审中-公开
标题翻译：热稳定木聚糖酶用于选择性水解含戊糖的多糖
公开(公告)号：WO2011080078A2
公开(公告)日：2011-07-07
申请号：PCT/EP2010/069736
申请日：2010-12-15
申请人： SÜD-CHEMIE AG , KOHL, Andreas , UNTERSTRASSER, Isabel , RARBACH, Markus , KOLTERMANN, Andre , REISINGER, Christoph , BRÜCK, Thomas , KETTLING, Ulrich , HREGGVIDSSON, Gudmundur Oli , FRIDJONSSON, Olafur Hedinn
发明人： KOHL, Andreas , UNTERSTRASSER, Isabel , RARBACH, Markus , KOLTERMANN, Andre , REISINGER, Christoph , BRÜCK, Thomas , KETTLING, Ulrich , HREGGVIDSSON, Gudmundur Oli , FRIDJONSSON, Olafur Hedinn
IPC分类号： C12N15/09
CPC分类号： C12N9/248
摘要： The present invention relates to polypeptides having xylanase activity and nucleic acid sequences encoding such polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides. One specific application of the xylanase is the selective hydrolysis of pentose sugar components of hemicelluiose-containing plant biomass. The nucleotide sequence may be used for the production of the xylanase or optimized mutants thereof.
摘要翻译：本发明涉及具有木聚糖酶活性的多肽和编码这些多肽的核酸序列。本发明还涉及包含核酸构建体的核酸构建体，载体和宿主细胞以及生产和使用该多肽的方法。木聚糖酶的一个具体应用是选择性水解含半纤维素的植物生物质的戊糖组分。该核苷酸序列可用于生产木聚糖酶或其优化的突变体。

3. 发明申请

WO2010136404A3 LIQUEFIED BIOMASS 审中-公开
标题翻译：液化生物量
公开(公告)号：WO2010136404A3
公开(公告)日：2011-02-24
申请号：PCT/EP2010057045
申请日：2010-05-21
申请人： SUED CHEMIE AG , KOLTERMANN ANDRE , RARBACH MARKUS , BRUECK THOMAS , GERLACH JOCHEN , UNTERSTRASER ISABEL , KOHL ANDREAS , DRAGOVIC ZDRAVKO , KETTLING ULRICH
发明人： KOLTERMANN ANDRE , RARBACH MARKUS , BRUECK THOMAS , GERLACH JOCHEN , UNTERSTRASER ISABEL , KOHL ANDREAS , DRAGOVIC ZDRAVKO , KETTLING ULRICH
IPC分类号： C12P5/00
CPC分类号： C12P19/02 , C12P19/14 , Y02E50/17
摘要： The invention is directed to a liquefied sugar beet and/or sugar cane biomass material as well as production methods and uses thereof. The liquefied biomass is storage stable and can be used for the production of a product resulting from fermentation.
摘要翻译：本发明涉及液化甜菜和/或甘蔗生物质材料及其生产方法和用途。液化生物质储存稳定，可用于生产发酵产物。

4. 发明申请

WO2008113585A1 GENERATION OF CHEMICAL BUILDING BLOCKS FROM PLANT BIOMASS BY SELECTIVE DEPOLYMERIZATION 审中-公开
标题翻译：通过选择性分解从植物生物量产生化学建筑块
公开(公告)号：WO2008113585A1
公开(公告)日：2008-09-25
申请号：PCT/EP2008/002223
申请日：2008-03-19
申请人： SÜD-CHEMIE AG , KOLTERMANN, Andre , KETTLING, Ulrich , BRÜCK, Thomas , RARBACH, Markus
发明人： KOLTERMANN, Andre , KETTLING, Ulrich , BRÜCK, Thomas , RARBACH, Markus
IPC分类号： C12P19/02 , C13K1/02 , C13K1/06 , C07H1/08 , C12N9/24 , C12N9/42 , C12N9/02 , C12S3/04 , C12P7/08 , C12P19/12 , C12P19/14 , C12P19/16 , C12P19/18 , C12P19/20
CPC分类号： C12N9/2434 , C07H1/08 , C12N9/0061 , C12P19/02 , C12P19/12 , C12P19/14 , C12P19/16 , C12P19/18 , C12P19/20 , C12P19/22 , C12P19/24 , C13B35/005 , C13K1/02 , C13K1/06 , C13K13/002
摘要： The invention concerns a method for the enzymatic treatment of raw polymeric feedstock comprising the following steps: (a) preferably separation of soluble components from the raw polymeric feedstock, (b) treating the raw polymeric feedstock with an enzyme system in order to liberate defined soluble monomeric or oligomeric building blocks from the insoluble raw polymeric feedstock; and (c) separating the defined monomeric or oligomeric building blocks produced in step b) from the remainder of the raw polymeric feedstock. Preferably, the enzyme system used in step b) contains not more than 50%, preferably not more than 20%, more preferably not more than 10%, more preferably not more than 5%, more preferably not more than 2%, more preferably not more than 1 % of other enzyme activities apart from the enzyme activity resulting in liberation of said defined monomeric or oligomeric building blocks from the raw polymeric feedstock according to step b). Further aspects of the invention concern the use of "less pure" and thus less costly enzyme systems in subsequent enzymatic treatment steps and methods for determining the optimum sequence of enzymatic treatment steps by analysis of the raw polymeric feedstock used.
摘要翻译：本发明涉及用于酶处理原料聚合物原料的方法，包括以下步骤：（a）优选将可溶性组分与原料聚合原料分离，（b）用酶体系处理原料聚合物原料，以释放限定的可溶性来自不溶性原料聚合原料的单体或低聚结构单元; 和（c）将步骤b）中制备的限定的单体或低聚结构单元与原始聚合原料的其余部分分离。优选地，步骤b）中使用的酶系统含有不超过50％，优选不超过20％，更优选不超过10％，更优选不超过5％，更优选不超过2％不超过1％的其它酶活性，除了酶活性，导致根据步骤b）从所述原料聚合物原料释放所述限定的单体或低聚结构单元。本发明的另外方面涉及在随后的酶处理步骤中使用“较不纯”且因此较低成本的酶体系，以及通过分析所用原料聚合物原料来确定酶处理步骤的最佳顺序的方法。

5. 发明申请

WO2004113522A1 NEW BIOLOGICAL ENTITIES AND THE PHARMACEUTICAL OR DIAGNOSTIC USE THEREOF 审中-公开
标题翻译：新生物实验及其药物或诊断用途
公开(公告)号：WO2004113522A1
公开(公告)日：2004-12-29
申请号：PCT/EP2004/051173
申请日：2004-06-18
申请人： DIREVO BIOTECH AG , HAUPTS, Ulrich , KOLTERMANN, Andre , SCHEIDIG, Andreas , VOETSMEIER, Christian , KETTLING, Ulrich
发明人： HAUPTS, Ulrich , KOLTERMANN, Andre , SCHEIDIG, Andreas , VOETSMEIER, Christian , KETTLING, Ulrich
IPC分类号： C12N9/56
CPC分类号： C12N9/6427 , A61K38/482 , A61K38/4826 , A61K38/486 , A61K38/4873 , A61K38/488 , C12P21/06 , C12Q1/37
摘要： The present invention provides method for the treatment of a disease by applying a medicament comprising a protease with a defined specificity is capable to hydrolyze specific peptide bonds within a target substrate related to such disease. The proteases with such a defined specificity can further be used for related therapeutic or diagnostic purposes.
摘要翻译：本发明提供了通过应用包含具有确定的特异性的蛋白酶的药物能够水解与这种疾病相关的靶基质内的特异性肽键来治疗疾病的方法。具有这种确定的特异性的蛋白酶可进一步用于相关的治疗或诊断目的。

6. 发明申请

WO2003012100A2 METHOD FOR THE PRODUCTION OF NUCLEIC ACIDS CONSISTING OF STOCHASTICALLY COMBINED PARTS OF SOURCE NUCLEIC ACIDS 审中-公开
标题翻译：生产源于核苷酸的生物合成组分的核酸的方法
公开(公告)号：WO2003012100A2
公开(公告)日：2003-02-13
申请号：PCT/EP2002/008122
申请日：2002-07-20
申请人： DIREVO BIOTECH AG , KOLTERMANN, Andre , KETTLING, Ulrich , GREINER-STÖFFELE, Thomas , SPANGENBERG, Oliver
发明人： KOLTERMANN, Andre , KETTLING, Ulrich , GREINER-STÖFFELE, Thomas , SPANGENBERG, Oliver
IPC分类号： C12N15/10
CPC分类号： C12N15/1027
摘要： The present invention relates to a method for the production of nucleic acids consisting of stochastically combined parts of source nucleic acids as well as to a kit containing instructions for carrying out said method.
摘要翻译：本发明涉及由源核酸的随机组合部分组成的核酸的制备方法以及包含用于实施所述方法的说明书的试剂盒。

7. 发明申请

WO2011080078A3 THERMOSTABLE XYLANASE FOR THE SELECTIVE HYDROLYSIS OF PENTOSE-CONTAINING POLYSACCHARIDES 审中-公开
标题翻译：用于选择性水解含PENTOSE的多糖的热稳定性XYLANASE
公开(公告)号：WO2011080078A3
公开(公告)日：2011-10-13
申请号：PCT/EP2010069736
申请日：2010-12-15
申请人： SUED CHEMIE AG , KOHL ANDREAS , UNTERSTRASSER ISABEL , RARBACH MARKUS , KOLTERMANN ANDRE , REISINGER CHRISTOPH , BRUECK THOMAS , KETTLING ULRICH , HREGGVIDSSON GUDMUNDUR OLI , FRIDJONSSON OLAFUR HEDINN
发明人： KOHL ANDREAS , UNTERSTRASSER ISABEL , RARBACH MARKUS , KOLTERMANN ANDRE , REISINGER CHRISTOPH , BRUECK THOMAS , KETTLING ULRICH , HREGGVIDSSON GUDMUNDUR OLI , FRIDJONSSON OLAFUR HEDINN
IPC分类号： C07K14/195 , C07K14/00
CPC分类号： C12N9/248 , C07K14/195
摘要： The present invention relates to polypeptides having xylanase activity and nucleic acid sequences encoding such polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides. One specific application of the xylanase is the selective hydrolysis of pentose sugar components of hemicelluiose-containing plant biomass. The nucleotide sequence may be used for the production of the xylanase or optimized mutants thereof.
摘要翻译：本发明涉及具有木聚糖酶活性的多肽和编码此类多肽的核酸序列。本发明还涉及包含核酸构建体的核酸构建体，载体和宿主细胞以及用于产生和使用多肽的方法。木聚糖酶的一个具体应用是含有半纤维素的植物生物质的戊糖糖成分的选择性水解。核苷酸序列可用于生产木聚糖酶或其优化的突变体。

8. 发明申请

WO2010076305A1 PROCESS FOR CELL-FREE PRODUCTION OF CHEMICALS 审中-公开
标题翻译：无细胞生产化学品的方法
公开(公告)号：WO2010076305A1
公开(公告)日：2010-07-08
申请号：PCT/EP2009/067954
申请日：2009-12-28
申请人： SÜD-CHEMIE AG , KOLTERMANN, Andre , KETTLING, Ulrich , SIEBER, Volker
发明人： KOLTERMANN, Andre , KETTLING, Ulrich , SIEBER, Volker
IPC分类号： C12P7/06 , C12P7/16
CPC分类号： C12P7/16 , C12P7/06 , Y02E50/10 , Y02E50/17
摘要： An enzymatic process is described for the production of chemicals from carbon sources. In particular, according to one aspect, a process for the production of a target organic compound from a carbon source by a cell-free enzyme system is disclosed.
摘要翻译：描述了从碳源生产化学品的酶法。特别地，根据一个方面，公开了一种通过无细胞酶系统从碳源生产目标有机化合物的方法。

9. 发明申请

WO2010009958A1 ABBAU VON KOHLENHYDRATHALTIGEN MATERIALIEN MIT ANORGANISCHEN KATALYSATOREN 审中-公开
标题翻译：去除效果无机催化剂碳水化合物含材料的
公开(公告)号：WO2010009958A1
公开(公告)日：2010-01-28
申请号：PCT/EP2009/058144
申请日：2009-06-30
申请人： SÜD-CHEMIE AG , KETTLING, Ulrich , KOLTERMANN, Andre , KRAUS, Michael
发明人： KETTLING, Ulrich , KOLTERMANN, Andre , KRAUS, Michael
IPC分类号： C13K1/04 , C10G1/08
CPC分类号： C13K1/04 , C13K1/02
摘要： Die Erfindung betrifft ein Verfahren zur Depolymerisation kohlenhydrathaltiger Materialen umfassend die folgenden Schritte: (a) Behandlung eines kohlenhydrathaltigen Materials mit einem anorganischen Katalysator, um definierte monomere oder oligomere Bausteine aus dem kohlenhydrathaltigen Material freizusetzen; und (b) Abtrennung der in Schritt (a) erzeugten definierten monomeren oder oligomeren Bausteine von dem Rest des kohlenhydrathaltigen Materials. Vorzugsweise umfasst der in Schritt (a) eingesetzte anorganische Katalysator Tectosilicate, Phyllosilicate oder Hydrotalcite weiter bevorzugt Zeolithe oder Bentonite. Des Weiteren umfassen das kohlenhydrathaltige Material bevorzugt LCB und die definierten monomeren oder oligomeren Bausteinen vorzugsweise Glucose, Xylose, Arabinose und deren Oligomere. Weitere Aspekte der Erfindung betreffen die Verwendung von Lösungsvermittlern in Kombination mit dem anorganischen Katalysator.
摘要翻译：本发明涉及一种用于包括以下步骤的含碳水化合物的材料的解聚的方法包括：（a）处理含碳水化合物的材料与无机催化剂以释放定义从含碳水化合物的材料的单体或低聚积木; 和（b）在步骤分离（a）中生成的所定义的含碳水化合物的材料的剩余部分的单体或低聚的构建块。优选地，在步骤（a）优选使用无机催化剂包括网状硅酸盐，层状硅酸盐或水滑石进一步沸石或膨润土。还包括含碳水化合物的材料是优选LCB和所定义的单体或低聚的积木优选葡萄糖，木糖，阿拉伯糖，和它们的低聚物。本发明的进一步的方面涉及在组合使用增溶剂与无机催化剂。

10. 发明申请

WO2006067198A3 TARGETED USE OF ENGINEERED ENZYMES 审中-公开
公开(公告)号：WO2006067198A3
公开(公告)日：2006-06-29
申请号：PCT/EP2005/057061
申请日：2005-12-21
申请人： DIREVO BIOTECH AG , VOETSMEIER, Christian , HAUPTS, Ulrich , KOLTERMANN, Andre , SCHEIDIG, Andreas , KETTLING, Ulrich , COCO, Wayne Michael
发明人： HAUPTS, Ulrich , KOLTERMANN, Andre , SCHEIDIG, Andreas , KETTLING, Ulrich , COCO, Wayne Michael
IPC分类号： A61K38/43 , C12N9/00
摘要： The present invention provides method for the treatment of a disease by applying a medicament comprising a protease with a defined specificity is capable to hydrolyze specific peptide bonds within a target substrate related to such disease. The proteases with such a defined specificity can further be used for related therapeutic or diagnostic purposes.

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