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1. 发明申请

WO2012138629A2 AGROBACTERIUM-MEDIATED TRANSFORMATION OF DICOT PLANTS 审中-公开
标题翻译： DICOT植物的农杆菌介导转化
公开(公告)号：WO2012138629A2
公开(公告)日：2012-10-11
申请号：PCT/US2012031947
申请日：2012-04-03
申请人： PIONEER HI BRED INT , DU PONT , CHO HYEON-JE , CASTLE LINDA A , KLEIN THEODORE MITCHELL , MINH TAI , MOY YORK
发明人： CHO HYEON-JE , CASTLE LINDA A , KLEIN THEODORE MITCHELL , MINH TAI , MOY YORK
IPC分类号： A01H1/00 , C12N5/04 , C12N15/82
CPC分类号： C12N15/8205 , C12N1/20
摘要： Compositions and methods are provided for the efficient transformation of a dicot plant. More particularly, compositions and methods of the present invention find use in agriculture for Agrobacterium-mediated transformation of a dicotyledonous plant. The compositions include cultivation media comprising high concentrations of sucrose and glucose. The cultivation media find use in methods directed to Agrobacterium-mediated transformation of a dicot plant with a gene of interest. In this manner, any gene of interest can be introduced into a dicot plant with high transformation efficiency and reduced tissue necrosis.
摘要翻译：提供组合物和方法用于双子叶植物的有效转化。更具体地，本发明的组合物和方法用于农业用于双子叶植物的农杆菌介导的转化。组合物包括含有高浓度蔗糖和葡萄糖的培养基。栽培培养基可用于涉及农杆菌介导的具有感兴趣基因的双子叶植物转化的方法。以这种方式，可以将任何感兴趣的基因导入具有高转化效率和减少的组织坏死的双子叶植物。

2. 发明申请

WO0031269A3 PLANT CELL PROLIFERATION-ASSOCIATED PROTEINS 审中-公开
标题翻译：植物细胞增殖相关蛋白
公开(公告)号：WO0031269A3
公开(公告)日：2000-10-05
申请号：PCT/US9927550
申请日：1999-11-19
申请人： DU PONT , PIONEER HI BRED INT , CAHOON REBECCA E , KLEIN THEODORE M , WENG ZUDE , LOWE KEITH S
发明人： CAHOON REBECCA E , KLEIN THEODORE M , WENG ZUDE , LOWE KEITH S
IPC分类号： C07K14/415 , C12N15/29 , C12N15/82 , C12Q1/02 , C12Q1/68 , G01N33/68
CPC分类号： C12N15/8241 , C07K14/415 , C12N15/8261 , Y02A40/146
摘要： This invention relates to an isolated nucleic acid fragment encoding a protein involved in cell division and proliferation. The invention also relates to the construction of a chimeric gene encoding all or a portion of the protein involved in cell division and proliferation, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the protein involved in cell division and proliferation in a transformed host cell.
摘要翻译：本发明涉及编码参与细胞分裂和增殖的蛋白质的分离的核酸片段。本发明还涉及以正义或反义方向构建编码参与细胞分裂和增殖的全部或部分蛋白质的嵌合基因，其中嵌合基因的表达导致产生改变水平的参与细胞的蛋白质在转化的宿主细胞中分裂和增殖。

3. 发明申请

WO9947689A3 INHIBITORS OF APOPTOSIS PROTEINS IN PLANTS 审中-公开
标题翻译：植物蛋白酶抑制剂
公开(公告)号：WO9947689A3
公开(公告)日：1999-11-25
申请号：PCT/US9905227
申请日：1999-03-10
申请人： DU PONT , CAHOON REBECCA E , CAIMI PERRY G , KLEIN THEODORE M , MORGANTE MICHELE , SAKAI HAJIME , SHEN JENNIE BIH JIEN , TINGEY SCOTT V
发明人： CAHOON REBECCA E , CAIMI PERRY G , KLEIN THEODORE M , MORGANTE MICHELE , SAKAI HAJIME , SHEN JENNIE BIH-JIEN , TINGEY SCOTT V
IPC分类号： C07K14/415 , C12N15/29 , C12N15/82 , C12N5/10 , C12Q1/68
CPC分类号： C12N15/8263 , C07K14/415
摘要： This invention relates to an isolated nucleic acid fragment encoding an apoptosis inhibitory protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the apoptosis inhibitory protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the apoptosis inhibitory protein in a transformed host cell.
摘要翻译：本发明涉及编码凋亡抑制蛋白的分离的核酸片段。本发明还涉及在正义或反义方向上构建编码全部或部分凋亡抑制蛋白的嵌合基因，其中嵌合基因的表达导致在转化的宿主细胞中产生改变的凋亡抑制蛋白的水平。

4. 发明申请

WO1997022703A2 NOVEL STARCHES VIA MODIFICATION OF EXPRESSION OF STARCH BIOSYNTHETIC ENZYME GENES 审中-公开
标题翻译：通过淀粉酶生物合成酶基因表达修饰的新方法
公开(公告)号：WO1997022703A2
公开(公告)日：1997-06-26
申请号：PCT/US1996019678
申请日：1996-12-12
申请人： E.I. DU PONT DE NEMOURS AND COMPANY , HUBBARD, Natalie, Louise , KLEIN, Theodore, Mitchell , BROGLIE, Karen, E.
发明人： E.I. DU PONT DE NEMOURS AND COMPANY
IPC分类号： C12N15/54
CPC分类号： C12N9/107 , A23L29/212 , C12N15/8245
摘要： The instant invention discloses utilization of a cDNA clone to construct sense and antisense genes for inhibition of starch branching enzyme enzymatic activity in corn. More specifically, this invention concerns a method of controlling the starch fine structure of starch derived from the grain of corn comprising: (1) preparing a chimeric gene comprising a nucleic acid fragment encoding a starch branching enzyme structural gene or a fragment thereof, operably linked in either sense or antisense orientation on the upstream side to a nucleic acid fragment encoding a promoter that directs gene expression in corn endosperm tissue, and operably linked on the downstream side to a nucleic acid fragment encoding a suitable regulatory sequence for transcriptional termination, and (2) transforming corn with said chimeric gene, wherein expression of said chimeric gene results in alteration of the fine structure of starch derived from the grain of said transformed corn compared to the fine structure of starch derived from corn not possessing said chimeric gene.
摘要翻译：本发明公开了利用cDNA克隆构建用于抑制玉米淀粉分支酶酶活性的有义和反义基因。更具体地说，本发明涉及一种控制来自玉米粒的淀粉的淀粉精细结构的方法，包括：（1）制备嵌合基因，其包含编码淀粉分支酶结构基因或其片段的核酸片段，其可操作地连接在上游侧的任何正义或反义方向，编码引导玉米胚乳组织中的基因表达的启动子的核酸片段，并且在下游侧可操作地连接到编码转录终止的合适调控序列的核酸片段，和（ 2）用所述嵌合基因转化玉米，其中所述嵌合基因的表达导致衍生自所述转化玉米的淀粉的淀粉的精细结构的改变与源自不具有所述嵌合基因的玉米的淀粉的精细结构相反。

5. 发明申请

WO2013033308A3 METHODS FOR TISSUE CULTURE AND TRANSFORMATION OF SUGARCANE 审中-公开
公开(公告)号：WO2013033308A3
公开(公告)日：2013-03-07
申请号：PCT/US2012/053003
申请日：2012-08-30
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , CHO, Myeong-Je , KLEIN, Theodore M. , ZHAO, Zuo-Yu
发明人： CHO, Myeong-Je , KLEIN, Theodore M. , ZHAO, Zuo-Yu
IPC分类号： C12N15/82 , A01H4/00
摘要： Compositions and methods for the efficient transformation and regeneration of monocot plants are provided. The methods of transformation involve infection with Agrobacterium . In this manner, any gene of interest can be introduced into the monocot plant with high transformation efficiency and in low copy number. Transformed and regenerated monocot cells, tissues, plants, and seed are also provided. The invention encompasses regenerating transformed plants, transgenic seeds produced therefrom, and transgenic plants and transgenic seeds from subsequent generations.

6. 发明申请

WO2005059148A1 RECOMBINASE MEDIATED GENE TRAPS 审中-公开
标题翻译：重组介导的基因TRAPS
公开(公告)号：WO2005059148A1
公开(公告)日：2005-06-30
申请号：PCT/US2004/042232
申请日：2004-12-16
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS AND COMPANY , GORDON-KAMM, William J. , LYZNIK, L. Alexander , SCELONGE, Christopher J. , KLEIN, Theodore M.
发明人： GORDON-KAMM, William J. , LYZNIK, L. Alexander , SCELONGE, Christopher J. , KLEIN, Theodore M.
IPC分类号： C12N15/82
CPC分类号： C12N15/8213 , C12N15/8209
摘要： The present invention provides methods for identifying a genetically modified plant cell comprising a recombinase-mediated exchange between a first nucleotide sequence and a second nucleotide sequence. The present invention provides a method for obtaining a genetically modified plant cell wherein a functional recombination at both the 5' and 3' ends of the nucleotide can be identified.
摘要翻译：本发明提供用于鉴定遗传修饰的植物细胞的方法，所述植物细胞包含第一核苷酸序列和第二核苷酸序列之间的重组酶介导的交换。本发明提供了获得遗传修饰的植物细胞的方法，其中可以鉴定核苷酸的5'和3'末端的功能重组。

7. 发明申请

WO2004067723A2 FLORAL DEVELOPMENT GENES 审中-公开
标题翻译：花卉发展基因
公开(公告)号：WO2004067723A2
公开(公告)日：2004-08-12
申请号：PCT/US2004/002422
申请日：2004-01-29
申请人： PIONEER HI-BRED INTERNATIONAL INC. , E. I. DU PONT DE NEMOURS & CO. , DANILEVSKAYA, Olga , HERMON, Pedro , BRUGGEMANN, Edward , SHIRBROUN, David , ANANIEV, Evgueni , RAFALSKI, Jan Antoni , SAKAI, Hajime , CAHOON, Edgar , CAHOON, Rebecca , KLEIN, Theodore
发明人： DANILEVSKAYA, Olga , HERMON, Pedro , BRUGGEMANN, Edward , SHIRBROUN, David , ANANIEV, Evgueni , RAFALSKI, Jan Antoni , SAKAI, Hajime , CAHOON, Edgar , CAHOON, Rebecca , KLEIN, Theodore
IPC分类号： C12N
CPC分类号： C12N15/8214 , C07K14/415 , C12N15/8261 , C12N15/8287 , C12N15/8289 , Y02A40/146
摘要： This invention relates to an isolated nucleic acid fragment encoding floral development proteins, more specifically FT, TFL or Ap3 homologs. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the floral development proteins, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the FT, TFL or Ap3 homologs in a transformed host cell.
摘要翻译：本发明涉及编码花发育蛋白的分离的核酸片段，更具体地说是FT，TFL或Ap3同源物。本发明还涉及在正义或反义方向上构建编码全部或部分花发育蛋白的重组DNA构建体，其中重组DNA构建体的表达导致产生改变的FT，TFL或Ap3同源物的水平在转化的宿主细胞中。

8. 发明申请

WO0244390A3 FLORAL DEVELOPMENT GENES 审中-公开
标题翻译：花卉发展基因
公开(公告)号：WO0244390A3
公开(公告)日：2003-09-12
申请号：PCT/US0143750
申请日：2001-11-21
申请人： DU PONT , CAHOON EDGAR B , CAHOON REBECCA E , KLEIN THEODORE M , RAFALSKI ANTONI J , SAKAI HAJIME
发明人： CAHOON EDGAR B , CAHOON REBECCA E , KLEIN THEODORE M , RAFALSKI ANTONI J , SAKAI HAJIME
IPC分类号： C07K14/415 , C12N15/29 , C12N15/82 , A01H5/00 , C12N5/10
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8214 , C12N15/8287 , C12N15/8289 , Y02A40/146
摘要： This invention relates to an isolated nucleic acid fragment encoding a floral development proteins, more specifically a FT or Ap3 homolog. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the floral development proteins, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the FT or Ap3 homolog in a transformed host cell.
摘要翻译：本发明涉及编码花发育蛋白的分离的核酸片段，更具体地，涉及FT或Ap3同源物。本发明还涉及在正义或反义方向上构建编码全部或一部分花发育蛋白质的重组DNA构建体，其中重组DNA构建体的表达导致产生改变的FT或Ap3同源物水平转化的宿主细胞。

9. 发明申请

WO2003002751A2 ALTERATION OF OIL TRAITS IN PLANTS 审中-公开
标题翻译：植物油田改造
公开(公告)号：WO2003002751A2
公开(公告)日：2003-01-09
申请号：PCT/US2002/020152
申请日：2002-06-27
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL, INC. , ALLEN, Stephen, M. , ALLEN, William, B. , CAHOON, Rebecca, E. , EPELBAUM, Sabine , FAMODU, Omolayo, O. , HARVELL, Leslie, T. , JONES, Todd, J. , KINNEY, Anthony, J. , KLEIN, Theodore, M. , LI, Changjiang , OLIVEIRA, Igor, Cunha , SAKAI, Hajime , SHEN, Bo , TARCZYNSKI, Mitchell, C.
发明人： ALLEN, Stephen, M. , ALLEN, William, B. , CAHOON, Rebecca, E. , EPELBAUM, Sabine , FAMODU, Omolayo, O. , HARVELL, Leslie, T. , JONES, Todd, J. , KINNEY, Anthony, J. , KLEIN, Theodore, M. , LI, Changjiang , OLIVEIRA, Igor, Cunha , SAKAI, Hajime , SHEN, Bo , TARCZYNSKI, Mitchell, C.
IPC分类号： C12Q
CPC分类号： C12N15/8247 , C07K14/415
摘要： The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Chimeric construct incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.
摘要翻译：公开了可用于改变植物油表型的核酸片段的制备和使用。掺入此类核酸片段和合适调节序列的嵌合构建体可用于产生具有改变的脂质分布的转基因植物。还公开了使用这种核酸片段改变植物油表型的方法。

10. 发明申请

WO9722703A3 NOVEL STARCHES VIA MODIFICATION OF EXPRESSION OF STARCH BIOSYNTHETIC ENZYME GENES 审中-公开
标题翻译：通过淀粉酶生物合成酶基因表达修饰的新方法
公开(公告)号：WO9722703A3
公开(公告)日：1997-07-24
申请号：PCT/US9619678
申请日：1996-12-12
申请人： DU PONT , HUBBARD NATALIE LOUISE , KLEIN THEODORE MITCHELL , BROGLIE KAREN E
发明人： HUBBARD NATALIE LOUISE , KLEIN THEODORE MITCHELL , BROGLIE KAREN E
IPC分类号： A23L1/0522 , C12N9/10 , C12N15/29 , C12N15/54 , C12N15/82 , A01H5/00 , C08B30/00
CPC分类号： C12N9/107 , A23L29/212 , C12N15/8245
摘要： The instant invention discloses utilization of a cDNA clone to construct sense and antisense genes for inhibition of starch branching enzyme enzymatic activity in corn. More specifically, this invention concerns a method of controlling the starch fine structure of starch derived from the grain of corn comprising: (1) preparing a chimeric gene comprising a nucleic acid fragment encoding a starch branching enzyme structural gene or a fragment thereof, operably linked in either sense or antisense orientation on the upstream side to a nucleic acid fragment encoding a promoter that directs gene expression in corn endosperm tissue, and operably linked on the downstream side to a nucleic acid fragment encoding a suitable regulatory sequence for transcriptional termination, and (2) transforming corn with said chimeric gene, wherein expression of said chimeric gene results in alteration of the fine structure of starch derived from the grain of said transformed corn compared to the fine structure of starch derived from corn not possessing said chimeric gene.

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