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1. 发明申请

WO2003030817A2 ANTIBODY COMPLEXES AND METHODS FOR IMMUNOLABELING 审中-公开
标题翻译：抗体复合物和免疫结合方法
公开(公告)号：WO2003030817A2
公开(公告)日：2003-04-17
申请号：PCT/US2002/031416
申请日：2002-10-02
申请人： MOLECULAR PROBES, INC. , ARCHER, Robert, A. , BEECHEM, Joseph, M. , HAGEN, David, C. , HAUGLAND, Richard, P. , HAUGLAND, Rosaria, P.
发明人： ARCHER, Robert, A. , BEECHEM, Joseph, M. , HAGEN, David, C. , HAUGLAND, Richard, P. , HAUGLAND, Rosaria, P.
IPC分类号： A61K
CPC分类号： B82Y30/00 , B82Y5/00 , B82Y10/00 , G01N33/53 , G01N33/58 , G01N33/6857
摘要： The present invention provides labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling proteins have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Typically, the labeling reagent is an anti-Fc Fab or Fab' fragment that was generated by immunizing a goat or rabbit with the Fc fragment of an antibody. The present invention provides for discrete subsets of labeling reagent and immuno-labeled complexes that facilitate the simultaneous detection of multiple targets in a sample wherein the immuno-labeled complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeling reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.
摘要翻译：本发明提供标记试剂和用于标记初级抗体并用于使用包含靶标结合抗体和一种或多种标记试剂的免疫标记复合物检测样品中的靶标的方法。标记试剂包含单价抗体片段或非抗体单体蛋白质，由此标记蛋白质对靶标结合抗体的特定区域具有亲和力且共价连接至标记。通常，标记试剂是通过用抗体的Fc片段免疫山羊或兔产生的抗Fc Fab或Fab'片段。本发明提供了标记试剂和免疫标记复合物的离散子集，其便于同时检测样品中的多个靶标，其中免疫标记的复合物通过以下方式来区分：i）标记与标记试剂的比例，或ii）物理所述标记的性质，或iii）标记试剂与所述靶标结合抗体的比率，或者iv）所述靶标结合抗体。这对于荧光团标记是特别有用的，所述标记可以连接到标记试剂上，并且随后用于检测多个靶标的比率的免疫标记复合物。

2. 发明申请

WO2003030817A3 ANTIBODY COMPLEXES AND METHODS FOR IMMUNOLABELING 审中-公开
公开(公告)号：WO2003030817A3
公开(公告)日：2003-04-17
申请号：PCT/US2002/031416
申请日：2002-10-02
申请人： MOLECULAR PROBES, INC. , ARCHER, Robert, A. , BEECHEM, Joseph, M. , HAGEN, David, C. , HAUGLAND, Richard, P. , HAUGLAND, Rosaria, P.
发明人： ARCHER, Robert, A. , BEECHEM, Joseph, M. , HAGEN, David, C. , HAUGLAND, Richard, P. , HAUGLAND, Rosaria, P.
IPC分类号： G01N33/58
摘要： The present invention provides labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling proteins have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Typically, the labeling reagent is an anti-Fc Fab or Fab' fragment that was generated by immunizing a goat or rabbit with the Fc fragment of an antibody. The present invention provides for discrete subsets of labeling reagent and immuno-labeled complexes that facilitate the simultaneous detection of multiple targets in a sample wherein the immuno-labeled complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeling reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.

3. 发明申请

WO03030817B1 ANTIBODY COMPLEXES AND METHODS FOR IMMUNOLABELING 审中-公开
标题翻译：抗体复合物和免疫标记方法
公开(公告)号：WO03030817B1
公开(公告)日：2003-11-06
申请号：PCT/US0231416
申请日：2002-10-02
申请人： MOLECULAR PROBES INC , ARCHER ROBERT A , BEECHEM JOSEPH M , HAGEN DAVID C , HAUGLAND RICHARD P , HAUGLAND ROSARIA P
发明人： ARCHER ROBERT A , BEECHEM JOSEPH M , HAGEN DAVID C , HAUGLAND RICHARD P , HAUGLAND ROSARIA P
IPC分类号： G01N33/53 , G01N33/58 , G01N33/68
CPC分类号： B82Y30/00 , B82Y5/00 , B82Y10/00 , G01N33/53 , G01N33/58 , G01N33/6857
摘要： The present invention provides labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling proteins have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Typically, the labeling reagent is an anti-Fc Fab or Fab' fragment that was generated by immunizing a goat or rabbit with the Fc fragment of an antibody. The present invention provides for discrete subsets of labeling reagent and immuno-labeled complexes that facilitate the simultaneous detection of multiple targets in a sample wherein the immuno-labeled complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeling reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.
摘要翻译：本发明提供标记试剂和用于标记一抗的标记试剂和使用包含靶结合抗体和一种或多种标记试剂的免疫标记复合物检测样品中靶标的方法。标记试剂包括单价抗体片段或非抗体单体蛋白质，由此标记的蛋白质对靶标结合抗体的特定区域具有亲和性，并且共价连接到标记物上。通常，标记试剂是通过用抗体的Fc片段免疫山羊或兔而产生的抗Fc Fab或Fab'片段。本发明提供标记试剂和免疫标记的复合物的离散子集，其有助于同时检测样品中的多个靶标，其中通过i）标记物与标记试剂的比率来区分免疫标记的复合物，或ii）物理或iii）标记试剂与所述靶结合抗体的比例，或iv）所述靶结合抗体。这对于可以连接到标记试剂和随后的用于检测多个靶的比例的免疫标记的复合物的荧光团标记物特别有用。

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