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    • 8. 发明申请
    • A METHOD FOR IDENTIFYING ENZYME INHIBITORS
    • 鉴定酶抑制剂的方法
    • WO0244408A2
    • 2002-06-06
    • PCT/GB0105329
    • 2001-12-03
    • ARROW THERAPEUTICS LTDCHARLES IAN GEORGERICHARDS JAMIEMASKELL DUNCAN JOHN
    • CHARLES IAN GEORGERICHARDS JAMIEMASKELL DUNCAN JOHN
    • C12Q1/25C12Q1/68G01N33/50
    • C12Q1/25G01N2500/04
    • A method for screening a compound for its ability to inhibit DNA adenine methylase (Dam) is disclosed. The method utilizes a double stranded DNA comprising at least one Dam target site. One end of the DNA is bound to a solid support such as the well of a microtitre plate. The other end is provided with a means for detecting whether the double stranded DNA comprising the Dam target site has been cleaved. In the assay, a compound to be tested is added to the well of the microtitre plate. Dam and a substrate therefor are added and if the compound is not an inhibitor of Dam the Dam will methylate the adenine residue of the Dam target site. By adding an enzyme that selectively cleaves the double stranded DNA at the Dam target site but which will not cleave a methylated Dam target site (or vice versa) it is possible to selectively cleave the detection means from the DNA. If the DNA is cleaved the detection means will be lost in a subsequent wash step whereas if the DNA is not cleaved the detection means will remain bound to the microtitre plate and can be used to generate, for example, a colour reaction which can in turn be used to determine whether the compound under test is an inhibitor of Dam. Also disclosed is a kit for conducting such an assay.
    • 公开了一种筛选化合物抑制DNA腺嘌呤甲基化酶(Dam)能力的方法。 该方法利用包含至少一个Dam靶位点的双链DNA。 DNA的一端与固体支持物结合,例如微量滴定板的孔。 另一端设置有用于检测包含Dam靶位点的双链DNA是否已被切割的装置。 在测定中,将待测试的化合物加入到微量滴定板的孔中。 添加水坝和底物,如果化合物不是Dam的抑制剂,则Dam将使Dam目标部位的腺嘌呤残基甲基化。 通过添加在Dam靶位点选择性切割双链DNA但不会切割甲基化的Dam靶位点(或反之亦然)的酶,可以从DNA选择性地切割检测手段。 如果DNA被切割,检测手段将在随后的洗涤步骤中丢失,而如果DNA未被切割,则检测装置将保持与微量滴定板结合,并且可用于产生例如可以反过来进行的着色反应 用于确定被测化合物是否是Dam的抑制剂。 还公开了用于进行这种测定的试剂盒。
    • 10. 发明申请
    • ARMOUR IMPROVEMENTS
    • 武装改进
    • WO2011103614A1
    • 2011-09-01
    • PCT/AU2011/000106
    • 2011-02-03
    • GORDON, Duncan, John
    • GORDON, Duncan, John
    • F41H7/04F41H5/007F41H5/04
    • F41H5/04
    • An improvement for armour panel that includes a first main section having a plurality of adjacent compartments, each compartment being at least partially filled with a flowable material. As a projectile attempts to pass through the armour panel it is subjected to a range of different densities and surface interactions with the flowable material depending on its compositions well as having an increased likelihood of striking the inner surfaces of the compartments, which can be of a honeycomb structure. The adjacent compartments can be interconnected to allow the flowable material to circulate
    • 一种护甲板的改进,其包括具有多个相邻隔室的第一主要部分,每个隔室至少部分地填充有可流动的材料。 当射弹试图通过护甲板时,根据组合物的不同密度和与可流动材料的表面相互作用很好地受到一定程度的冲击,这可能是打开隔室的内表面的可能性, 蜂窝结构。 相邻的隔室可以互连以允许可流动的材料循环