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1. 发明申请

WO2002059333A3 GENE PROMOTERS ISOLATED FROM POTATO AND USE THEREOF 审中-公开
公开(公告)号：WO2002059333A3
公开(公告)日：2002-08-01
申请号：PCT/US2002/001287
申请日：2002-01-18
申请人： DAI, Ziyu , SHI, Lifang , HOOKER, Brian, S.
发明人： DAI, Ziyu , SHI, Lifang , HOOKER, Brian, S.
IPC分类号： C12N15/82
摘要： There are disclosed novel pin1 gene promoter isoforms and amt gene promoter isoforms. The promoters and their functional elements amy be used independently or in combination with one or more enhancer elements to increase or otherwise manipulate gene expression. The promoters disclosed may be used in Controlled Environment Agriculture fro heterlogous protein production. Also disclosed are methods for using the promoter and promoter elements of the instant invention, as well as vectors and transgenic plants comprising the same.

2. 发明申请

WO2007032751A3 ISOLATED FUNGAL PROMOTERS AND GENE TRANSCRIPTION TERMINATORS AND METHODS OF PROTEIN AND CHEMICAL PRODUCTION IN A FUNGUS 审中-公开
公开(公告)号：WO2007032751A3
公开(公告)日：2007-03-22
申请号：PCT/US2005/028651
申请日：2005-08-12
申请人： BATTELLE MEMORIAL INSTITUTE , DAI, Ziyu , LASRE, Linda, L. , MAGNUSON, Jon, K.
发明人： DAI, Ziyu , LASRE, Linda, L. , MAGNUSON, Jon, K.
IPC分类号： C12N15/80
摘要： The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

3. 发明申请

WO2005123928A1 PRODUCTION OF HUMAN COAGULATION FACTOR VIII FROM PLANT CELLS AND WHOLE PLANTS 审中-公开
标题翻译：从植物细胞和全植物生产人类凝血因子VIII
公开(公告)号：WO2005123928A1
公开(公告)日：2005-12-29
申请号：PCT/US2004/018312
申请日：2004-06-08
申请人： BATTELLE MEMORIAL INSTITUTE , HOOKER, Brian , ANDERSON, Daniel , GAO, Johnway , DAI, Ziyu
发明人： HOOKER, Brian , ANDERSON, Daniel , GAO, Johnway , DAI, Ziyu
IPC分类号： C12N15/82
CPC分类号： C12N15/8257 , C12N5/04 , C12N2510/02
摘要： The invention includes methods for production of a polypeptide having factor VIII activity by introduction of a polynucleotide construct into a plant cell. The construct includes an encoding sequence for a polypeptide of coagulation factor VIII or a functional variant thereof. The plant cell is cultured or regenerated into a plant and the polypeptide or functional variant of factor VIII is expressed therein. The invention also includes vectors, plant cells, plant tissues, plants and seeds containing a polynucleotide sequence encoding a functional variant of human coagulation factor VIII. The invention further includes a recombinant DNA molecule having a promoter which is functional in plants operably linked to a coding sequence which codes for a polynucleotide having coagulation factor VIII activity.
摘要翻译：本发明包括通过将多核苷酸构建体引入植物细胞来生产具有因子VIII活性的多肽的方法。该构建体包含凝血因子VIII多肽或其功能变体的编码序列。将植物细胞培养或再生成植物，并且在其中表达因子VIII的多肽或功能变体。本发明还包括含有编码人凝血因子VIII功能变体的多核苷酸序列的载体，植物细胞，植物组织，植物和种子。本发明还包括具有启动子的重组DNA分子，所述启动子在植物中可操作地连接编码具有凝血因子VIII活性的多核苷酸的编码序列。

4. 发明申请

WO2002070649A3 EPIMERASE GENE AND USE THEREOF 审中-公开
公开(公告)号：WO2002070649A3
公开(公告)日：2002-09-12
申请号：PCT/US2002/002727
申请日：2002-02-01
申请人： DAI, Ziyu , SHI, Lifang , HOOKER, Brian, S.
发明人： DAI, Ziyu , SHI, Lifang , HOOKER, Brian, S.
IPC分类号： A01H5/00
摘要： This invention provides transgenic plants that have increased or decreased activity or uridine 5' -diphospho-galactose 4-epimerase. Controlling the level of uridine 5' -diphospho-galactose 4-epimerase in the transgenic plants is used to regulate the nutritional profile of the plant by controlling the use of glucose and/or galactose. An isolated polynucleotide coding for the potato uridine 5' -diphospho-galactose 4-epimerase protein, its antisense equivalent and the nucleic acid sequence of potato uridine 5' -diphospho-galactose --epimerase are also provided. The present invention also provides a method for reducing the activity or uridine 5' -diphospho-galactose 4-epimerase activity in plants. The present invention also provides a method of producing bulk quantities of uridine 5' -diphospho-galactose 4-epimerase enzyme in transgenic plants.

5. 发明申请

WO2002059333A2 GENE PROMOTERS ISOLATED FROM POTATO AND USE THEREOF 审中-公开
标题翻译：从POTATO分离的基因促进剂及其用途
公开(公告)号：WO2002059333A2
公开(公告)日：2002-08-01
申请号：PCT/US2002/001287
申请日：2002-01-18
申请人： DAI, Ziyu , SHI, Lifang , HOOKER, Brian, S.
发明人： DAI, Ziyu , SHI, Lifang , HOOKER, Brian, S.
IPC分类号： C12N15/82
CPC分类号： C23C16/45565 , C12N9/1096 , C12N15/8237 , C12N15/8238 , C23C16/45519
摘要： There are disclosed novel pin1 gene promoter isoforms and amt gene promoter isoforms. The promoters and their functional elements amy be used independently or in combination with one or more enhancer elements to increase or otherwise manipulate gene expression. The promoters disclosed may be used in Controlled Environment Agriculture fro heterlogous protein production. Also disclosed are methods for using the promoter and promoter elements of the instant invention, as well as vectors and transgenic plants comprising the same.
摘要翻译：已经公开了新的pin1基因启动子同种型和amt基因启动子同种型。启动子及其功能元件可以单独使用或与一种或多种增强子元件组合使用以增加或以其他方式操纵基因表达。所公开的启动子可以用于杂交蛋白质生产中的受控环境农业。还公开了使用本发明的启动子和启动子元件以及包含其的载体和转基因植物的方法。

6. 发明申请

WO0136649A3 ISOLATION AND CHARACTERIZATION OF THE GENOMIC DNA CLONES OF RIBOSOMAL PROTEIN GENE L25 IN TOBACCO 审中-公开
标题翻译： RIBOSOMAL蛋白基因L25在烟草中的基因组DNA克隆的分离和表征
公开(公告)号：WO0136649A3
公开(公告)日：2002-01-03
申请号：PCT/US0031716
申请日：2000-11-20
申请人： BATTELLE MEMORIAL INSTITUTE , SHI LI FANG , GAO JOHNWAY , HOOKER BRIAN S , DAI ZIYU
发明人： SHI LI-FANG , GAO JOHNWAY , HOOKER BRIAN S , DAI ZIYU
IPC分类号： C12N15/29 , C12N15/82 , A01H5/00 , C12N5/10
CPC分类号： C12N15/8225 , C12N15/8234
摘要： The promoter of the ribosomal protein gene L25 is operably linked to a structural gene. This chimeric gene is placed in an expression vector. The expression vector containing the chimeric gene is used to transform plants cells and plants. Seeds are obtained from the transformed plants. A product, such as a protein, encoded by the structural gene is isolated from the transformed plant cells and plants.
摘要翻译：核糖体蛋白基因L25的启动子与结构基因可操作地连接。将该嵌合基因置于表达载体中。含有嵌合基因的表达载体用于转化植物细胞和植物。从转化的植物获得种子。从转化的植物细胞和植物中分离由结构基因编码的产物，如蛋白质。

7. 发明申请

WO02059333A9 GENE PROMOTERS ISOLATED FROM POTATO AND USE THEREOF 审中-公开
标题翻译：基因启动子从马铃薯中分离并使用
公开(公告)号：WO02059333A9
公开(公告)日：2002-11-28
申请号：PCT/US0201287
申请日：2002-01-18
申请人： DAI ZIYU , SHI LIFANG , HOOKER BRIAN S
发明人： DAI ZIYU , SHI LIFANG , HOOKER BRIAN S
IPC分类号： C12N9/10 , C12N15/29 , C12N15/82 , C23C16/44 , C23C16/455 , A01H5/00 , C12N5/10
CPC分类号： C23C16/45565 , C12N9/1096 , C12N15/8237 , C12N15/8238 , C23C16/45519
摘要： There are disclosed novel pin1 gene promoter isoforms and amt gene promoter isoforms. The promoters and their functional elements amy be used independently or in combination with one or more enhancer elements to increase or otherwise manipulate gene expression. The promoters disclosed may be used in Controlled Environment Agriculture fro heterlogous protein production. Also disclosed are methods for using the promoter and promoter elements of the instant invention, as well as vectors and transgenic plants comprising the same.
摘要翻译：已经公开了新颖的pin1基因启动子同种型和amt基因启动子同种型。启动子及其功能元件可独立使用或与一种或多种增强子元件组合使用以增加或以其他方式操作基因表达。所公开的启动子可用于受控环境农业中的异源蛋白质生产。还公开了使用本发明的启动子和启动子元件的方法，以及包含它们的载体和转基因植物。

8. 发明申请

WO02070649A2 EPIMERASE GENE AND USE THEREOF 审中-公开
标题翻译： EPIMERASE基因及其用途
公开(公告)号：WO02070649A2
公开(公告)日：2002-09-12
申请号：PCT/US0202727
申请日：2002-02-01
申请人： DAI ZIYU , SHI LIFANG , HOOKER BRIAN S
发明人： DAI ZIYU , SHI LIFANG , HOOKER BRIAN S
IPC分类号： C12N9/80 , C12N
CPC分类号： C12Y305/01003 , C12N9/80
摘要： This invention provides transgenic plants that have increased or decreased activity or uridine 5' -diphospho-galactose 4-epimerase. Controlling the level of uridine 5' -diphospho-galactose 4-epimerase in the transgenic plants is used to regulate the nutritional profile of the plant by controlling the use of glucose and/or galactose. An isolated polynucleotide coding for the potato uridine 5' -diphospho-galactose 4-epimerase protein, its antisense equivalent and the nucleic acid sequence of potato uridine 5' -diphospho-galactose --epimerase are also provided. The present invention also provides a method for reducing the activity or uridine 5' -diphospho-galactose 4-epimerase activity in plants. The present invention also provides a method of producing bulk quantities of uridine 5' -diphospho-galactose 4-epimerase enzyme in transgenic plants.
摘要翻译：本发明提供具有增加或降低的活性的转基因植物或尿苷5'-二磷酸 - 半乳糖-4-差向异构酶。通过控制葡萄糖和/或半乳糖的使用，可以控制转基因植物中尿苷5'-二磷酸 - 半乳糖4-差向异构酶的水平来调节植物的营养特征。还提供了编码马铃薯尿苷5'-二磷酸 - 半乳糖-4-差向异构酶蛋白质的分离的多核苷酸，其反义等同物和马铃薯尿苷5'-二磷酸 - 半乳糖 - 同工酶的核酸序列。本发明还提供了降低植物中活性或尿苷5'-二磷酸 - 半乳糖4-差向异构酶活性的方法。本发明还提供了一种在转基因植物中生产大量尿苷5'-二磷酸 - 半乳糖-4-差向异构酶的方法。

9. 发明申请

WO0127297A3 VERSATILE rpL34 PROMOTER ELEMENTS AND USE THEREOF 审中-公开
标题翻译：多用途rpL34推动元件及其用途
公开(公告)号：WO0127297A3
公开(公告)日：2001-12-27
申请号：PCT/US0028111
申请日：2000-10-12
申请人： BATTELLE MEMORIAL INSTITUTE , SHI LIFANG , DAI ZIYU , GAO JOHNWAY , HOOKER BRIAN S , ANDERSON DANIEL B
发明人： SHI LIFANG , DAI ZIYU , GAO JOHNWAY , HOOKER BRIAN S , ANDERSON DANIEL B
IPC分类号： C12N15/29 , C12N15/82 , C12P21/02
CPC分类号： C12N15/8238 , C12N15/8216 , C12N15/8229 , C12N15/8237 , C12N15/8239
摘要： Synergistically functional, yet separable, cis -acting enhancer elements from the rpL34 promoter are disclosed. These enhancer elements of the instant invention may be used in combination with a plurality of promoters to increase gene expression without affecting the intrinsic specificity of the promoters. Also disclosed are methods for using the enhancer elements of the instant invention as well as vectors and transgenic plants comprising the enhancer elements.
摘要翻译：公开了来自rpL34启动子的协同功能但可分离的顺式增强子元件。本发明的这些增强子元件可以与多种启动子组合使用以增加基因表达而不影响启动子的固有特异性。还公开了使用本发明增强子元件的方法以及包含增强子元件的载体和转基因植物。

10. 发明申请

WO2008028019A1 METHODS AND MICROORGANISMS FOR FORMING FERMENTATION PRODUCTS AND FIXING CARBON DIOXIDE 审中-公开
标题翻译：形成发酵产品和固定二氧化碳的方法和微生物
公开(公告)号：WO2008028019A1
公开(公告)日：2008-03-06
申请号：PCT/US2007/077198
申请日：2007-08-30
申请人： BATTELLE MEMORIAL INSTITUTE , DAI, Ziyu , LASURE, Linda, L. , BAKER, Scott, E. , MAGNUSON, Jon, K.
发明人： DAI, Ziyu , LASURE, Linda, L. , BAKER, Scott, E. , MAGNUSON, Jon, K.
IPC分类号： C12P7/06 , C12N1/19
CPC分类号： C12P7/06 , C12N9/88 , Y02E50/17
摘要： Methods and microorganisms for forming fermentation products utilizing a microorganism having at least one heterologous gene sequence that enables carbohydrate conversion and carbon dioxide fixation in the production of the fermentation products are disclosed according to some aspects.
摘要翻译：根据一些方面，公开了利用具有至少一种能够在生产发酵产物中进行碳水化合物转化和二氧化碳固定的异源基因序列的微生物形成发酵产物的方法和微生物。

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