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1. 发明申请

WO2013138255A1 INTEGRATED BIOREFINERY 审中-公开
标题翻译：一体化生物
公开(公告)号：WO2013138255A1
公开(公告)日：2013-09-19
申请号：PCT/US2013/030296
申请日：2013-03-11
申请人： COBALT TECHNOLOGIES INC.
发明人： BURNS-GUYDISH, Stacy, M. , .CANN, Anthony F , DALAL, Dhruti , FRY, Lawrence W. , HERSHKOWITZ, Michael S. , MCDONALD, William F. , MEYER, Andrew D. , NAVA-SALGADO, Victor O. , OLSON, Brandon T. , SONICO, Ishmael M. , WALTHER, David C. , ZHU, Yongming
IPC分类号： C12P7/14
CPC分类号： C12P7/14 , C12M21/12 , C12M23/42 , C12M33/00 , C12M43/02 , C12M45/00 , C12M45/06 , C12M45/20 , C12N1/14 , C12N1/20 , C12P7/10 , C12P7/16 , C12P7/28 , C12P2203/00 , Y02E50/10 , Y02E50/16
摘要： Systems and methods for producing bioproducts are shown. The systems and methods herein can be configured and used in an integrated biorefinery. The integrated biorefinery may comprise a sugar production facility such as a sugar mill, a production facility for one or more bioproduct(s) such as butanol, and optionally an ethanol production facility employing the system and method.
摘要翻译：显示生产生物产品的系统和方法。本文中的系统和方法可以在一个综合生物精炼厂中进行配置和使用。综合生物精炼可以包括糖生产设备，例如糖厂，用于一种或多种生物产物例如丁醇的生产设备，以及任选地使用该系统和方法的乙醇生产设备。

2. 发明申请

WO2010126848A1 MULTISTAGE VAPOR COMPRESSION DISTILLATION 审中-公开
标题翻译：多级蒸汽压缩蒸馏
公开(公告)号：WO2010126848A1
公开(公告)日：2010-11-04
申请号：PCT/US2010/032463
申请日：2010-04-26
申请人： COBALT TECHNOLOGIES, INC. , KAUFMAN, Bruce, D. , WALTHER, David, C. , CONTAG, Pamela, Reilly
发明人： KAUFMAN, Bruce, D. , WALTHER, David, C. , CONTAG, Pamela, Reilly
IPC分类号： B01D3/00
CPC分类号： B01D1/2887 , B01D3/007
摘要： The invention provides systems and methods for multistage vapor compression distillation (VCD). A plurality of VCD units may be combined to form a VCD device where heat from a prior VCD unit may be transferred to a subsequent VCD unit. Each VCD unit may have an evaporator stage and a condenser stage where heat from the condenser stage of a prior VCD unit may be transferred to the evaporator stage of the subsequent VCD unit. The amount of heat transfer between units may be controlled. A multistage VCD device may be used to separate an acetone-butanol-ethanol fermentation broth.
摘要翻译：本发明提供多级蒸气压缩蒸馏（VCD）的系统和方法。可以组合多个VCD单元以形成VCD设备，其中来自先前的VCD单元的热量可以被传送到后续的VCD单元。每个VCD单元可以具有蒸发器级和冷凝器级，其中来自先前VCD单元的冷凝器级的热量可以被传送到随后的VCD单元的蒸发器级。可以控制单元之间的热传递量。可以使用多级VCD装置来分离丙酮 - 丁醇 - 乙醇发酵液。

3. 发明申请

WO2010027531A1 ENGINEERED LIGHT-EMITTING REPORTER GENES AND MEDTHODS OF USE 审中-公开
标题翻译：工程发光报告基因和使用药物
公开(公告)号：WO2010027531A1
公开(公告)日：2010-03-11
申请号：PCT/US2009/036868
申请日：2009-03-11
申请人： COBALT TECHNOLOGIES, INC. , BURNS-GUYDLSH, Stacy, M. , CONTAG, Pamela, Reilly
发明人： BURNS-GUYDLSH, Stacy, M. , CONTAG, Pamela, Reilly
IPC分类号： C12N15/31 , C12N15/63 , C12N1/21 , C12R1/145
CPC分类号： C12P7/16 , C12N1/20 , C12N15/01 , C12N15/1058 , C12N15/1086 , Y02E50/10
摘要： Compositions and methods are provided for enhanced expression of light emitting reporters. Such reporters are used in methods for monitoring cultures for production of target compounds. Additionally, methods are provided for the use of light emitting reporter systems and other reporter systems for selecting desired traits in an organism and for identifying or optimizing culture conditions.
摘要翻译：提供组合和方法以增强发光报告器的表达。这样的记者用于监测用于生产目标化合物的培养物的方法。另外，提供了使用发光报道系统和其他报道系统来选择生物体中期望的性状并用于鉴定或优化培养条件的方法。

4. 发明申请

WO2009120806A3 EXPRESSION OF OXIDOREDUCTASES TO INCREASE VOLUMETRIC PRODUCTIVITY 审中-公开
标题翻译：氧化反应的表达增加体积生产力
公开(公告)号：WO2009120806A3
公开(公告)日：2010-02-25
申请号：PCT/US2009038300
申请日：2009-03-25
申请人： COBALT TECHNOLOGIES INC , CONTAG PAMELA REILLY , BURNS-GUYDISH STACY M , MEERMAN HENDRIKUS J
发明人： CONTAG PAMELA REILLY , BURNS-GUYDISH STACY M , MEERMAN HENDRIKUS J
IPC分类号： C12N1/21 , C12N9/02 , C12N15/53 , C12P7/06 , C12P7/16 , C12R1/145
CPC分类号： C12P7/16 , C12N9/0004 , C12N9/0069 , C12P7/065 , C12P7/28 , Y02E50/10 , Y02E50/17
摘要： Compositions and methods are provided for increased volumetric productivity of microorganisms by expression of oxidoreductases. Such methods are useful in increasing the volumetric production of alcohols by microorganisms such as Clostridium.
摘要翻译：提供了通过氧化还原酶的表达提高微生物的体积生产率的组合物和方法。这样的方法可用于通过诸如梭菌（Clostridium）的微生物增加醇的体积产生。

5. 发明申请

WO2009033114A2 ENGINEERED LIGHT-EMITTING REPORTER GENES 审中-公开
标题翻译：工程发光报告基因
公开(公告)号：WO2009033114A2
公开(公告)日：2009-03-12
申请号：PCT/US2008075515
申请日：2008-09-06
申请人： COBALT TECHNOLOGIES INC , BURNS-GUYDISH STACY M , CONTAG PAMELA REILLY
发明人： BURNS-GUYDISH STACY M , CONTAG PAMELA REILLY
IPC分类号： C12N15/11 , C12N1/21 , C12N9/02 , C12N15/00 , C12P21/04 , C12Q1/66
CPC分类号： C12N15/74 , C12N1/20 , C12N9/0069 , C12N15/01 , C12N15/1058 , C12P7/16 , Y02E50/10
摘要： Compositions and methods are provided for enhanced expression of light emitting reporters. Such reporters are used in methods for monitoring cultures for production of target compounds.
摘要翻译：提供组合和方法以增强发光报告器的表达。这样的记者用于监测用于生产目标化合物的培养物的方法。

6. 发明申请

WO2008082726A2 REAL TIME MONITORING OF MICROBIAL ENZYMATIC PATHWAYS 审中-公开
标题翻译：微生物酶通路的实时监测
公开(公告)号：WO2008082726A2
公开(公告)日：2008-07-10
申请号：PCT/US2007078321
申请日：2007-09-12
申请人： COBALT TECHNOLOGIES INC , CONTAG PAMELA REILLY
发明人： CONTAG PAMELA REILLY
IPC分类号： C12Q1/66
CPC分类号： C12Q1/6897 , C12N15/70 , C12N15/74 , C12N15/81 , C12P7/06 , C12P7/16 , C12P7/28 , C12P7/52 , C12P7/54 , C12P7/56 , G06Q99/00 , Y02E50/10 , Y02E50/17
摘要： This invention provides compositions and methods for monitoring and regulating the production of a target product of a biochemical pathway in an organism, such as butanol. A gene encoding a light-emitting reporter molecule, such as luciferase, is operatively linked with a transcription regulatory nucleotide sequence that regulates transcription of an enzyme in the pathway that signals the rate of production of the target product, such as butanol dehyrogenase. When a microorganism is transfected with such a reporter construct and cultured, the reporter is expressed contemporaneously with the enzyme. The amount of light produced by the reporter indicates amount of enzyme being produced which, in turn, signals the amount of target product being produced. When the reporter is measured in real time, it provides information that can be used to regulate culture conditions and to optimize production of the target product.
摘要翻译：本发明提供了用于监测和调节生物体例如丁醇中生物化学途径的目标产物的产生的组合物和方法。编码发光报道分子例如萤光素酶的基因与转录调节核苷酸序列可操作地连接，所述转录调节核苷酸序列调节信号通路中的酶转录，所述信号表示目标产物例如丁醇脱氢酶的产生速率。当用这种报道构建体转染微生物并培养时，报道基因与酶同时表达。报道基因产生的光量表明产生的酶的量，其反过来表示正在产生的目标产物的量。当记者实时测量时，它提供的信息可用于调节培养条件并优化目标产品的生产。

7. 发明申请

WO2015142399A1 PREPARATION OF BIOMASS 审中-公开
标题翻译：生物量的准备
公开(公告)号：WO2015142399A1
公开(公告)日：2015-09-24
申请号：PCT/US2014/072278
申请日：2014-12-23
申请人： COBALT TECHNOLOGIES, INC.
发明人： ZHU, Yongming , WALTHER, David C. , GAO, Xiadi
IPC分类号： C12P7/06 , C12P7/14 , C12P19/00
CPC分类号： C12P7/16 , C12P19/02 , C12P2201/00 , Y02E50/10 , Y02E50/17
摘要： Methods and systems are provided for preparing biomass for downstream pretreatment operations to release sugar molecules. Biomass is prewashed with acid hydrolysate prior to pretreatment. In so doing, the amount of chemicals that are required to efficiently process biomass for downstream uses is greatly reduced.
摘要翻译：提供了用于制备用于下游预处理操作释放糖分子的生物质的方法和系统。在预处理之前，用酸水解产物预生物质。这样做，大大减少了为下游用途有效处理生物质所需的化学物质量。

8. 发明申请

WO2015066316A1 BIOPRODUCT RECOVERY USING TRIGGERABLE POLYMERIC MATERIALS 审中-公开
标题翻译：使用可触发的聚合材料进行生物反应回收
公开(公告)号：WO2015066316A1
公开(公告)日：2015-05-07
申请号：PCT/US2014/063139
申请日：2014-10-30
申请人： COBALT TECHNOLOGIES, INC.
发明人： KAMBAM, Pavan , WRIGHT, Michael E.
IPC分类号： C12P7/28
CPC分类号： C12P7/16 , B01D15/30 , C07C29/76 , Y02E50/10 , C07C31/12
摘要： Systems, methods, and apparatus are provided for the removal and isolation and purification of bioproducts (e.g., alcohols) from a solution during and/or after production by chemical and/or biochemical production pathways. The disclosed systems and methods include absorption and/or adsorption of a bioproduct of interest by a polymeric resin followed by release of the bioproduct with a triggering agent or event such as modification of pH.
摘要翻译：提供了系统，方法和装置，用于通过化学和/或生物化学生产途径在制造期间和/或之后从溶液中除去和分离和纯化生物产物（例如醇）。所公开的系统和方法包括通过聚合物树脂吸收和/或吸附感兴趣的生物产物，然后用引发剂或事件如pH的改变释放生物产物。

9. 发明申请

WO2013019822A1 REMOVAL OF MICROBIAL FERMENTATION INHIBITORS FROM CELLULOSIC HYDROLYSATES OR OTHER INHIBITOR-CONTAINING COMPOSITIONS 审中-公开
标题翻译：从纤维素水解物或其他含有抑制剂的组合物中去除微生物发酵抑制剂
公开(公告)号：WO2013019822A1
公开(公告)日：2013-02-07
申请号：PCT/US2012/049084
申请日：2012-07-31
申请人： COBALT TECHNOLOGIES, INC. , KEATING, Jeffrey, D.
发明人： KEATING, Jeffrey, D.
IPC分类号： C12P7/16
CPC分类号： C12P7/16 , C12N1/38 , C12P2203/00 , Y02E50/10
摘要： Methods are provided for conditioning a cellulosic biomass hydrolysate to remove or inactivate inhibitors of microbial growth and/or metabolite production. The methods include precipitation or inactivation of inhibitors with calcium hydroxide and potassium hydroxide.
摘要翻译：提供了用于调节纤维素生物质水解产物以除去或失活微生物生长和/或代谢物产生抑制剂的方法。该方法包括用氢氧化钙和氢氧化钾沉淀或灭活抑制剂。

10. 发明申请

WO2011163620A1 REMOVAL OF INHIBITORS OF MICROBIAL FERMENTATION FROM INHIBITOR-CONTAINING COMPOSITIONS 审中-公开
标题翻译：从含有抑制剂的组合物中去除微生物发酵抑制剂
公开(公告)号：WO2011163620A1
公开(公告)日：2011-12-29
申请号：PCT/US2011/041879
申请日：2011-06-24
申请人： COBALT TECHNOLOGIES, INC. , HOGG, Eamon , LULI, Gregory, W. , WALTHER, David, C. , ECKERT, Robert , KEATING, Jeffrey, D.
发明人： HOGG, Eamon , LULI, Gregory, W. , WALTHER, David, C. , ECKERT, Robert , KEATING, Jeffrey, D.
IPC分类号： B01D61/00 , C02F1/44
CPC分类号： C12N1/20 , C12N1/22 , C12P7/16 , Y02E50/10 , Y02E50/17
摘要： Methods are provided for conditioning an inhibitor-containing composition, such as a cellulosic biomass hydrolysate, to remove inhibitors of microbial growth and/or product production. The methods include precipitation of inhibitors by formation of complexes with metal salts, such as aluminum sulfate and ferric chloride.
摘要翻译：提供了用于调节含抑制剂的组合物（例如纤维素生物质水解产物）以除去微生物生长和/或产物生产抑制剂的方法。所述方法包括通过与金属盐如硫酸铝和氯化铁形成络合物来沉淀抑制剂。

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