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    • 4. 发明申请
    • NOVEL NON-REVERTING SALMONELLA LIVE VACCINES
    • 新型非反流型SALMONELLA LIVE VACCINES
    • WO1986003123A1
    • 1986-06-05
    • PCT/US1985002260
    • 1985-11-15
    • THE BOARD OF TRUSTEES OF THE LELAND STANFORD JR. ...
    • THE BOARD OF TRUSTEES OF THE LELAND STANFORD JR. ...STOCKER, Bruce, A., D.
    • A61K39/112
    • C12N15/00A61K39/0275A61K2039/522C07K14/255C12R1/42Y02A50/482Y02A50/484Y10S435/879
    • Live vaccines and methods for preparing the live vaccines for protection of a host from a pathogenic microorganism. The vaccines are prepared by introducing at least one modification on a gene involved in at least one, normally at least two, biosynethetic pathways, involving the production of products which are unlikely to be found in the disease susceptible host. The modification results in a gene change which cannot be repaired by a single step e.g. polynucleotide deletions and inversions. Where the aro gene suffers such a change, the resultant auxotrophic mutants require aromatic amino acids, p-aminobenzoic acid and 2,3-dihydroxybenzoic acid or a highly concentrated source of absorbable iron. The auxotrophic mutations have substantially reduced or nonexistent virulence, while retaining the desired immunogenicity to initiate the immunogenic response. Various techniques can be employed for providing the desired change.
    • 活疫苗和制备用于保护宿主免受致病微生物的活疫苗的方法。 通过在参与至少一个,通常至少两个生物合成途径的基因上引入至少一个修饰来制备疫苗,涉及产生在易感染的病毒宿主中不太可能发现的产物。 该修饰导致不能通过单个步骤修复的基因变化,例如, 多核苷酸缺失和反转。 在aro基因遭受这种变化的地方,所得到的营养缺陷型突变体需要芳香族氨基酸,对氨基苯甲酸和2,3-二羟基苯甲酸或高浓度的可吸收铁源。 营养缺陷型突变具有显着降低或不存在的毒力,同时保留所需的免疫原性以引发免疫原性应答。 可以采用各种技术来提供所需的变化。