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    • 2. 发明申请
    • AP-ECD METHODS AND APPARATUS FOR MASS SPECTROMETRIC ANALYSIS OF PEPTIDES AND PROTEINS
    • AP-ECD方法和装置用于肽和蛋白质的质谱分析
    • WO2010096904A1
    • 2010-09-02
    • PCT/CA2010/000215
    • 2010-02-23
    • THE UNIVERSITY OF BRITISH COLUMBIAROBB, Damon, B.
    • ROBB, Damon, B.
    • H01J49/04H01J49/26
    • H01J49/0054H01J49/0072H01J49/165
    • The present invention provides an improved in-source atmospheric pressure electron capture dissociation (AP-ECD) method and apparatus for mass spectrometric analysis of peptides and proteins. The invention uses an electrified sprayer to generate multiply-charged peptide/protein ions from a sample solution, a source of electrons for negative reagents, and a guide and a flow of gas for guiding positively charged ions from the electrified sprayer to a downstream reaction region within the guide, the reaction region being at or near atmospheric pressure and substantially free of the electric field from the electrified sprayer. In another embodiment of the invention the method uses electron transfer dissociation (ETD), in the event that anions are substituted for electrons as the negative reagents. Fragment ions exiting the reaction region are subsequently passed into a mass analyzer of a mass spectrometer for mass analysis of the ions.
    • 本发明提供改进的源极大气压电子捕获离解(AP-ECD)方法和用于肽和蛋白质的质谱分析的装置。 本发明使用电动喷雾器从样品溶液中产生多电荷肽/蛋白质离子,用于阴性试剂的电子源,以及用于将带正电荷的离子从电化喷雾器引导到下游反应区域的引导和流动气体 在引导下,反应区域处于或接近大气压并且基本上没有来自带电喷雾器的电场。 在本发明的另一个实施方案中,在阴离子被取代为阴性试剂的情况下,该方法使用电子转移离解(ETD)。 离开反应区域的片段离子随后进入质谱仪的质谱仪,用于质量分析离子。