会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 6. 发明申请
    • METHOD AND APPARATUS EMPLOYING MAGNETIC BEADS FOR LIGAND BINDING ASSAYS OF BIOLOGICAL SAMPLES
    • WO2020256695A1
    • 2020-12-24
    • PCT/US2019/037628
    • 2019-06-18
    • VIRGIN INSTRUMENTS CORPORATION
    • VESTAL, Marvin L.
    • G01N33/543G01N33/53
    • An apparatus for ligand binding of biological samples includes a bead well configured to confine a plurality of magnetic beads. A sample well comprises a filter bottom configured to contain samples of interest. A first magnetic bead picker captures magnetic beads from the bead well and releases the captured magnetic beads into the sample well. An incubator incubates the magnetic beads in the sample well binding the bait molecules to sample molecules contained in the sample of interest. A washer washes the incubated magnetic beads removing weakly bound sample molecules while retaining magnetic beads comprising strongly bound sample molecules. A second magnetic bead picker captures the magnetic beads comprising strongly bound sample molecules from the sample well and releases the captured magnetic beads comprising strongly bound samples onto a sample plate. A matrix material applicator deposits MALDI matrix material onto a surface of the sample plate. A MALDI-TOF mass spectrometer receives the sample plate with deposited MALDI matrix material and performs time-of-flight mass spectrometry on the strongly bound sample molecules, thereby generating mass spectra of the sample. A computer executes an algorithm using the mass spectra generated by the MALDI- TOF mass spectrometer to produce a ligand binding assay.