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1. 发明申请

WO2004065935A2 SYSTEM AND METHOD DETERMINING SIZES OF POLYNUCLEOTIDES 审中-公开
标题翻译：系统和方法确定多核苷酸的大小
公开(公告)号：WO2004065935A2
公开(公告)日：2004-08-05
申请号：PCT/US2004/001791
申请日：2004-01-23
申请人： SPECTRUMEDIX LLC , ZHAOWEI, Liu , YIQIONG, Wu
发明人： ZHAOWEI, Liu , YIQIONG, Wu
IPC分类号： G01N
CPC分类号： C12Q1/6816 , C12Q1/68 , C12Q2565/125 , C12Q2537/107 , C12Q2527/15 , C12Q2525/204
摘要： The present invention relates to a method of determining whether a length of a first polynucleotide is equal to (a) a length of a second polynucleotide or (b) a length of a third polynucleotide. A sample including the first polynucleotide is provided. A first portion of the sample is combined with an amount of the second polynucleotide to form a first mixture. A second portion of the sample is combined with an amount of the third polynucleotide to form a second, different mixture. The second polynucleotide includes at least 1 additional base than the third polynucleotide. The at least 1 additional base is located intermediate terminal ends of the second polynucleotide. First duplexes including the first polynucleotide and the second polynucleotide are prepared. Second duplexes including the first polynucleotide and the third polynucleotide are prepared. The first and second duplexes are subjected to temperature gradient electrophoresis to obtain electrophoresis data. The electrophoresis data is analyzed to determine whether the length of the first polynucleotide is equal to (a) the length of the second polynucleotide or (b) the length of the third polynucleotide.
摘要翻译：本发明涉及确定第一多核苷酸的长度是否等于（a）第二多核苷酸的长度或（b）第三多核苷酸的长度的方法。提供了包含第一多核苷酸的样品。将样品的第一部分与一定量的第二多核苷酸组合以形成第一混合物。样品的第二部分与一定量的第三多核苷酸组合以形成第二种不同的混合物。第二个多核苷酸包括比第三个多核苷酸至少1个碱基。至少另外1个碱基位于第二多核苷酸的中间末端。制备包括第一多核苷酸和第二多核苷酸的第一双链体。制备包括第一多核苷酸和第三多核苷酸的第二双链体。对第一和第二双链体进行温度梯度电泳以获得电泳数据。分析电泳数据以确定第一多核苷酸的长度是否等于（a）第二多核苷酸的长度，或（b）第三多核苷酸的长度。

2. 发明申请

WO2005012501A3 FREE-SOLUTION DNA SEQUENCING 审中-公开
公开(公告)号：WO2005012501A3
公开(公告)日：2005-02-10
申请号：PCT/US2004/025145
申请日：2004-08-02
申请人： SPECTRUMEDIX LLC , LOGE, Gary, W.
发明人： LOGE, Gary, W.
IPC分类号： C12Q1/68
摘要： A method for sequencing DNA includes binding each of a plurality of DNA fragments to a respective microparticle to prepare a plurality microparticle composites. DNA fragments bound to different microparticles differing in length by at least one base. The microparticle composites are subjected to electrophoresis. The separated microparticle composites are detected to obtain electrophoresis data. The sequence of the DNA fragments is determined from the electrophoresis data.

3. 发明申请

WO2005011867A2 PROCESSING PARTICLE-CONTAINING SAMPLES 审中-公开
标题翻译：加工颗粒样品
公开(公告)号：WO2005011867A2
公开(公告)日：2005-02-10
申请号：PCT/US2004/025181
申请日：2004-08-02
申请人： HANDYLAB, INC. , WU, Betty , HANDIQUE, Kalyan , PARUNAK, Gene , KEHRER, Aaron , GANESAN, Karthik
发明人： WU, Betty , HANDIQUE, Kalyan , PARUNAK, Gene , KEHRER, Aaron , GANESAN, Karthik
IPC分类号： B01L3/00
CPC分类号： C12Q1/6806 , B01L3/502707 , B01L3/502738 , B01L3/502753 , B01L2200/10 , B01L2300/0681 , B01L2300/0816 , B01L2300/087 , B01L2300/0887 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0677 , B33Y40/00 , B33Y80/00 , C12Q2523/109 , G01N1/40 , Y10T436/25 , Y10T436/25375
摘要： A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.
摘要翻译：微流体装置包括用于将含颗粒的液体样品输入到装置中的输入端口，保持构件和压力致动器。保持构件与输入端口连通并且构造成将含有颗粒的液体样品的颗粒与含有流体样品的颗粒的液体的第一部分空间分离。压力执行器将至少一些分离的颗粒与从颗粒分离的液体的第一部分的一部分重新组合。该装置还可以包括从保留构件接收颗粒和液体的裂解室。裂解室热裂解颗粒以释放其内容物。

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