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1. 发明申请

WO2018080925A1 METHODS OF DIFFERENTIATION TO NEURONAL CELLS AND KITS THEREFOR 审中-公开
标题翻译：分化为神经元细胞和试剂盒的方法
公开(公告)号：WO2018080925A1
公开(公告)日：2018-05-03
申请号：PCT/US2017/057641
申请日：2017-10-20
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： SHIN, Soojung , YAN, Yiping , BEACHAM, Daniel
IPC分类号： C12N5/0793 , C12N5/00
CPC分类号： C12N5/0619 , C12N5/0031 , C12N5/0037 , C12N5/0606 , C12N5/0696 , C12N2500/99 , C12N2501/42 , C12N2506/08
摘要： Embodiments herein provide methods of differentiating neural stem cells to neuronal cells while concomitantly retarding neural stem cell proliferation. Resultant cultures demonstrate reduced clumping of cells, increased purity of neuronal cells and accelerated electrophysiology as compared to control methods.
摘要翻译：这里的实施方式提供了将神经干细胞分化成神经元细胞同时阻滞神经干细胞增殖的方法。与对照方法相比，合成的培养物表现出细胞结块减少，神经元细胞纯度增加和电生理学加速。

2. 发明申请

WO2017205796A9 METHODS AND SYSTEMS FOR GRAPHICAL USER INTERFACES FOR BIOLOGICAL DATA 审中-公开
公开(公告)号：WO2017205796A9
公开(公告)日：2017-11-30
申请号：PCT/US2017/034776
申请日：2017-05-26
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： MAJUMDAR, Nivedita Sumi
IPC分类号： G06F19/26
摘要： In one exemplary embodiment, a method computer-implemented method of generating an outlier wheel data visualization for a graphical user interface, GUI, is provided. The method includes receiving emission data from a plurality of amplification reactions. The method further includes generating an outlier wheel data visualization using the emission data including a plurality of lines, where each line included in the outlier wheel data visualization represents emission data from an amplification reaction of the plurality of amplification reactions, and each line having a length and a visual indicator. The length of each line represents growth of intensity of fluorescence of the emission data and the visual indicator indicates the associated cycle number of the emission data. Further, each line is associated with an angular position so that the plurality of lines is configured in a circular shape. The method includes displaying the outlier wheel data visualization on a GUI.

3. 发明申请

WO2016060974A1 METHODS, SYSTEMS, AND COMPUTER-READABLE MEDIA FOR ACCELERATED BASE CALLING 审中-公开
标题翻译：方法，系统和用于加速基准电话的计算机可读介质
公开(公告)号：WO2016060974A1
公开(公告)日：2016-04-21
申请号：PCT/US2015/055070
申请日：2015-10-12
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： DONNET, Benjamin
IPC分类号： G06F19/20
CPC分类号： C12Q1/6869 , G06F19/20
摘要： Embodiments disclose methods, systems, and computer-readable media for accelerated base calling of sequencing data. These methods may be adapted to accelerate sequence determination for data arising from a variety of different nucleic acid sequencing platforms. In various embodiments, configurable logic circuits such as FPGAs and GPUs may be adapted to perform raw signal processing, basecalling, and/or sequence determination operations providing further enhancements to the sequence analysis methods.
摘要翻译：实施例公开了用于加速基站调度序列数据的方法，系统和计算机可读介质。这些方法可以适应于加速来自多种不同核酸测序平台的数据的序列确定。在各种实施例中，诸如FPGA和GPU之类的可配置逻辑电路可以适于执行原始信号处理，基准测试和/或序列确定操作，从而提供对序列分析方法的进一步增强。

4. 发明申请

WO2017083609A1 PREPARATION OF ELECTROPHORESIS GELS, AND RELATED DEVICES, SYSTEMS, AND METHODS 审中-公开
标题翻译：电泳凝胶的制备及相关器件，系统和方法
公开(公告)号：WO2017083609A1
公开(公告)日：2017-05-18
申请号：PCT/US2016/061476
申请日：2016-11-11
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： BULLOCH, Kyle , DILLER, Thomas
IPC分类号： G01N27/447
CPC分类号： G01N27/44747 , G01N27/44704 , G01N27/44782
摘要： An apparatus (200) for preparation of an electrophoresis slab gel may include a base (220) having an opening configured to receive a cassette (100) configured to contain an electrophoresis slab gel, a clamping mechanism (240) configured to move relative to the base (220) between an open position in which the clamping mechanism (240) permits insertion of a cassette into the base (220), and a closed position in which the clamping mechanism (240) is configured to clamp a cassette received in the base, a compressible pad (260) operatively coupled to the clamping mechanism in a position to compress against a cassette (100) received in the base (220) in the closed position of the clamping mechanism (240). The cassette (100) may include a first plate (11) and a second plate (12), and a spacer mechanism (13) separate from each of the first and second plates, the spacer mechanism configured to be positioned between the inner faces of the first plate and the second plate. When subjected to a clamping force exerted on the outer faces of the first and second plates, the spacer mechanism is configured to maintain a separation distance between the inner faces of the first and second plates, and provide a seal to prevent leakage of an electrophoresis gel solution introduced between the plates.
摘要翻译：用于制备电泳平板凝胶的装置（200）可以包括：具有开口的基座（220），所述开口构造成接收配置为容纳电泳平板凝胶的盒（100）;夹紧机构（ 240），所述闭合位置构造成相对于所述基座（220）在所述夹紧机构（240）允许将盒子插入所述基座（220）的打开位置与所述夹紧机构（240）配置于其中的闭合位置之间移动以夹紧容纳在底座中的盒子;在夹紧机构（240）的关闭位置中，可操作地联接到夹紧机构的可压缩垫（260）压靠容纳在基座（220）中的盒子（100）。盒（100）可包括第一板（11）和第二板（12）以及与第一板和第二板中的每一个分离的间隔机构（13），间隔机构构造成定位在第一板第一板和第二板。当受到施加在第一和第二板的外表面上的夹紧力时，间隔机构被构造成保持第一和第二板的内表面之间的分离距离，并且提供密封以防止电泳凝胶的泄漏解决方案介绍之间的板块。

5. 发明申请

WO2017069781A1 FILTER-BASED SYSTEM AND METHOD FOR EFFICIENT CAPTURE AND LYSIS OF SUSPENDED CELLS 审中-公开
标题翻译：基于滤波器的系统和方法，用于有效地捕获和分析悬浮细胞
公开(公告)号：WO2017069781A1
公开(公告)日：2017-04-27
申请号：PCT/US2015/057217
申请日：2015-10-23
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： MARTIN, Philip
IPC分类号： C12N1/06 , C12N15/10 , C12M1/00 , B01D61/14
CPC分类号： C12N1/06 , B01D61/147 , C12M47/04 , C12M47/06 , C12N15/1017
摘要： A cell lysis workflow involving a cell suspension that is passed through a filter in a first flow direction from a first side of the filter toward a second side thereof. The filter captures a plurality of cells on the first side. A lysis solution is passed through the filter in a second flow direction opposite the first direction, thereby dislodging the plurality of cells captured on the filter, and resuspending the plurality of cells in the lysis solution on the first side of the filter. The lysis solution lyses the plurality of cells to produce a cellular lysate. The cellular lysate is passed through the filter in the first flow direction.
摘要翻译：涉及细胞悬液的细胞溶解工作流程，所述细胞悬液沿第一流动方向从滤器的第一侧朝向其第二侧穿过过滤器。过滤器捕获第一侧上的多个细胞。裂解溶液沿与第一方向相反的第二流动方向通过过滤器，由此移除捕集在过滤器上的多个细胞，并将多个细胞重新悬浮在过滤器的第一侧上的裂解溶液中。裂解溶液裂解多个细胞以产生细胞裂解物。细胞裂解物以第一流动方向通过过滤器。

6. 发明申请

WO2013095729A1 SEQUENTIAL LATERAL FLOW CAPILLARY DEVICE FOR ANALYTE DETERMINATION 审中-公开
标题翻译：用于分析确定的顺序性流动毛细管装置
公开(公告)号：WO2013095729A1
公开(公告)日：2013-06-27
申请号：PCT/US2012/055676
申请日：2012-09-16
申请人： LIFE TECHNOLOGIES CORPORATION , SEMENOV, Eugene , GARGIR, Ari
发明人： SEMENOV, Eugene , GARGIR, Ari
IPC分类号： B01L3/00
CPC分类号： G01N1/28 , B01L3/5023 , B01L3/50273 , B01L2200/026 , B01L2200/12 , B01L2200/16 , B01L2300/0825 , B01L2400/0406
摘要： Disclosed is a lateral flow capillary device (24) and uses thereof comprising a unipath capillary flow matrix (18) and a plurality of reservoirs ((32a)(32b)(32c)) each in fluid communication with the capillary flow matrix (18). The device includes one or more pressure delivery systems ((36a)(36b)(36c)(38a)(38b)(38c)) configured to urge capillary flow matrix (18) toward a portion of the housing (40) in which it resides, such that a substantially uniform pressure is applied over a portion of the capillary flow matrix (18).
摘要翻译：公开了一种侧流毛细管装置（24）及其使用，其包括每个与毛细管流动基质（18）流体连通的单通道毛细管流动基质（18）和多个储存器（（32a）（32b）（32c）），。该装置包括一个或多个压力输送系统（（36a）（36b）（36c）（38b）（38c）（38c））（38c）（38c）（38c）（38c）（38c）），其构造成将毛细管流动基质（18）推向壳体使得在毛细管流动基质（18）的一部分上施加基本均匀的压力。

7. 发明申请

WO2011031497A2 QUANTITATIVE FLUORESCENT PROTEIN STANDARDS 审中-公开
标题翻译：定量荧光蛋白标准品
公开(公告)号：WO2011031497A2
公开(公告)日：2011-03-17
申请号：PCT/US2010/046695
申请日：2010-08-25
申请人： LIFE TECHNOLOGIES CORPORATION , DILLER, Thomas , UPDYKE, Timothy
发明人： DILLER, Thomas , UPDYKE, Timothy
IPC分类号： G01N33/68 , G01N33/52 , G01N33/58
CPC分类号： G01N33/6803 , B33Y80/00 , C07K1/26 , Y10T436/10 , Y10T436/105831
摘要： The present invention provides dual labeled protein standards useful for the simultaneous determination of the molecular weight of a subject protein as well as the relative mass (i.e., amount) of the subject protein present in an electrophoresis lane. The invention is also directed to methods suitable for the preparation of such dual labeled protein standards and to methods of using such dual labeled proteins to simultaneously determine the molecular weight and the relative amount of a subject protein. Further embodiments are directed to the use dual labeled protein standards to make a more accurate determination of the amount of a protein present in an electrophoresis lane. Yet further embodiments are directed to kits containing the presently described dual protein standards. Dual labeled protein standards made and used in accordance with the embodiments set forth herein may be used to simultaneously determine the molecular weight and the relative amount of a subject protein in real time.
摘要翻译：本发明提供了用于同时测定受试蛋白的分子量以及存在于电泳泳道中的受试蛋白的相对质量（即，量）的双重标记蛋白质标准。本发明还涉及适用于制备这种双重标记的蛋白质标准品的方法以及使用这种双标记蛋白质同时测定受试蛋白质的分子量和相对量的方法。进一步的实施方案涉及使用双重标记的蛋白质标准物来更准确地测定电泳泳道中存在的蛋白质的量。另外的实施方案涉及包含目前描述的双重蛋白质标准品的试剂盒。根据本文所述的实施方案制备和使用的双重标记的蛋白质标准物可用于同时确定受试蛋白质的分子量和相对量。

8. 发明申请

WO2019099891A1 STREAMLINED METHODS FOR MAKING LIQUID MEDIA 审中-公开
公开(公告)号：WO2019099891A1
公开(公告)日：2019-05-23
申请号：PCT/US2018/061603
申请日：2018-11-16
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： ALLES, Neva , EYCHNER, Deirdre , LORENZO, Gabrielle , REYNOLDS, Mary , GULDE, Paul , BONIFACE, Ryan , PHELPS, Mwita , KAHN, Matthew , FIKE, Richard
IPC分类号： C12N5/00
CPC分类号： C12N5/0031 , C12N5/0018 , C12N5/005 , C12N2500/10 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/36 , C12N2500/38 , C12N2500/46 , C12N2500/60 , C12N2510/02
摘要： Provided herein are, inter alia , methods for preparing a liquid cell culture media that has lesser lot-to-lot analytical variation, increased performance, and has lesser metal ion concentrations compared to a liquid media prepared by traditional methods. Such liquid media may be used for culturing cells, including but not limited to, recombinant cells.

9. 发明申请

WO2017066707A1 RIBONUCLEOPROTEIN TRANSFECTION AGENTS 审中-公开
标题翻译：核糖核蛋白转染剂
公开(公告)号：WO2017066707A1
公开(公告)日：2017-04-20
申请号：PCT/US2016/057224
申请日：2016-10-14
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： XU, Xin , LIANG, Xiquan , DE MOLLERAT DU JEU, Xavier , POTTER, Robert Jason
IPC分类号： A61K48/00 , C12N15/88
CPC分类号： C12N15/90 , A61K48/0033 , A61K48/005 , C12N9/22 , C12N15/88 , C12N2830/30 , C12Y301/00
摘要： Provided herein are compositions and methods useful, inter alia , for the delivery of ribonucleoprotein complexes (e.g., Cas9/guide RNA complexes) into cells. The compositions and methods provided herein are particularly useful for the delivery of ribonucleoprotein complexes into pluripotent cells and lymphatic cells.
摘要翻译：本文提供了用于将核糖核蛋白复合物（例如Cas9 /引导RNA复合物）递送入细胞的组合物和方法，尤其是用于其他方面的组合物和方法。本文提供的组合物和方法对于将核糖核蛋白复合物递送到多能细胞和淋巴细胞中是特别有用的。

10. 发明申请

WO2017011598A1 SYSTEM AND METHOD FOR IMPROVED TRANSIENT PROTEIN EXPRESSION IN CHO CELLS 审中-公开
标题翻译：用于在CHO细胞中改进的瞬时蛋白表达的系统和方法
公开(公告)号：WO2017011598A1
公开(公告)日：2017-01-19
申请号：PCT/US2016/042166
申请日：2016-07-13
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： ZMUDA, Jonathan , LIU, Chao Yan
IPC分类号： C12P21/02 , C12N5/00
CPC分类号： C12P21/02 , C07K14/505 , C07K16/00 , C07K2317/14 , C07K2317/21 , C07K2317/92 , C12N5/0043 , C12N2510/02 , C12N2511/00
摘要： The present invention is directed generally to systems and methods suitable for high level expression of recombinant proteins in suspension CHO cells. In particular, the invention allows introduction of the invention obviates the need to replace, replenish or supplement the growth medium during the procedure. The invention also relates to compositions and kits useful for culturing and transforming/transfecting suspension CHO cells.
摘要翻译：本发明一般涉及适用于悬浮CHO细胞中重组蛋白高水平表达的系统和方法。特别地，本发明允许本发明的引入消除了在该过程期间更换，补充或补充生长培养基的需要。本发明还涉及可用于培养和转化/转染悬浮液CHO细胞的组合物和试剂盒。

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