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    • 81. 发明申请
    • MAMMALIAN CELL EXPRESSION VECTORS AND UTILIZATION
    • MAMMALIAN细胞表达载体和利用
    • WO2010006415A1
    • 2010-01-21
    • PCT/CA2009/000908
    • 2009-06-30
    • ATGCELL INC.LI, QiangCHEN, Xing-Zhen
    • LI, QiangCHEN, Xing-Zhen
    • C12N15/85C12N15/65C12Q1/68
    • C12N15/85C12N2830/00C12N2840/203
    • An improved mammalian expression vector system which allows: (1) highly expressing exogenous proteins in host mammalian cells; (2) rapidly and efficiently screening recombinant stable cell lines expressing the gene of interest; (3) maintain sustainable expression and prevent gene silencing; and (4) effectively secreting proteins into media in some of cases. The entire expression vector system includes optimized promoters and core promoters, the use of special internal ribosome entry sites, integration of the bacterial backbone into the mammalian expression unit, multiple choices of selection markers, artificial matrix attachment region elements, effective secreting lead sequences and their 5' and 3' UTRs, and proper combinations of these expression elements.
    • 改进的哺乳动物表达载体系统,其允许:(1)在宿主哺乳动物细胞中高度表达外源蛋白; (2)快速有效地筛选表达目的基因的重组稳定细胞系; (3)保持可持续的表达,防止基因沉默; 和(4)在某些情况下,有效地将蛋白质分解成培养基。 整个表达载体系统包括优化的启动子和核心启动子,使用特殊的内部核糖体进入位点,细菌骨架与哺乳动物表达单元的整合,选择标记的多种选择,人造基质附着区域元件,有效分泌引导序列及其 5'和3'UTR,以及这些表达元件的适当组合。
    • 83. 发明申请
    • BALUN SIGNAL TRANSFORMER
    • BALUN信号变压器
    • WO2008130796A2
    • 2008-10-30
    • PCT/US2008/058967
    • 2008-04-01
    • FREESCALE SEMICONDUCTOR INC.LIU, LianjunLI, Qiang
    • LIU, LianjunLI, Qiang
    • H03H7/42H03H7/1775H03H2001/0078
    • A system 20 includes an unbalanced device 22, a balanced device 24, and a balun (balanced-unbalanced) signal transformer 26 interposed between devices 22 and 24. The balun signal transformer 26 includes a balanced external port section 32 formed by ports 40 and 42. The balun signal transformer 26 includes a symmetric transformer 48 having a balanced port 50 formed by terminals 52 and 54. Terminal 52 is electrically interconnected with port 40, and an inductor 64 is interposed between terminal 54 and port 42. The inductor 64 shifts a phase of a signal component 72 at terminal 54 to balance substantially one hundred eighty degrees out-of-phase with a signal component 70 at terminal 52.
    • 系统20包括不平衡装置22,平衡装置24和插入在装置22和24之间的平衡 - 不平衡转换器(平衡 - 不平衡)信号变压器26.平衡不平衡变换器信号变压器26包括由端口40和42形成的平衡外部端口部分32 平衡不平衡变压器信号变压器26包括具有由端子52和54形成的平衡端口50的对称变压器48.端子52与端口40电互连,并且电感器64插入在端子54和端口42之间。电感器64移位 在端子54处的信号部件72的相位相平衡,以平衡与端子52处的信号部件70基本上相差180度的异相。