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    • 74. 发明申请
    • IMMUNOAFFINITY ISOLATION OF MODIFIED PEPTIDES FROM COMPLEX MIXTURES
    • 从复合混合物中修饰的肽的免疫分离
    • WO2003000931A1
    • 2003-01-03
    • PCT/US2002/019480
    • 2002-06-20
    • CELL SIGNALING TECHNOLOGY, INC.RUSH, JohnZHANG, HuiZHA, XiangmingCOMB, Michael, J.TAN, Yi
    • RUSH, JohnZHANG, HuiZHA, XiangmingCOMB, Michael, J.TAN, Yi
    • C12Q1/68
    • C07K16/44
    • The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS-MS), and/or MS 3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.
    • 本发明提供了从复杂的肽混合物中分离修饰的肽的方法,所述方法包括以下步骤:(a)从生物体获得蛋白质制剂,其中所述制剂包含来自两种或更多种不同蛋白质的修饰肽; (b)使制剂与至少一种固定的修饰特异性抗体接触; 和(c)在步骤(b)中分离至少一种由固定的修饰特异性抗体特异性结合的修饰的肽。 该方法还可以包括以下步骤:(d)通过质谱(MS),串联质谱(MS-MS)和/或MS 3分析表征步骤(c)中分离的修饰肽,或 (e)使用搜索程序将在步骤(d)表征期间获得的修饰肽的光谱与已知肽序列的光谱基本上匹配,从而鉴定修饰肽的亲本蛋白。 还提供了包含修饰特异性抗体和针对新型UFD1和PTN6磷酸化位点的抗体的免疫亲和分离装置。
    • 75. 发明申请
    • COMBUSTION EMISSION ESTIMATION WITH FLAME SENSING SYSTEM
    • 火焰传感系统的燃烧排放估算
    • WO2004048853A2
    • 2004-06-10
    • PCT/US2003/025722
    • 2003-08-18
    • ABB INC.
    • ZHANG, HuiCHASE, Paul, H.NIZIOLEK, James, M.
    • F23N
    • F23N5/082F23N2029/08F23N2029/20
    • There is described a method and apparatus for controlling the combustion by-product formation rate in at least one burner of a fossil fuel fired power plant. The burner has an associated flame scanner which is focused on a small area of the burner flame to obtain an image signal of the flame. A flame signal that represents properties of temporal combustion in the visible light spectrum of the burner is generated from the image signal. Combustion turbulence at the burner is analyzed from the flame signal by a dynamic invariant that has a relationship to the combustion by-product values and different combustion by-product levels at the burner and the combustion turbulence is correlated to the combustion by-product formation rate at the burner.The method and apparatus can also be used to correlated the combustion turbulence at a multiplicity of burners to the associated combustion by-product formation rate.
    • 描述了用于控制化石燃料火力发电站的至少一个燃烧器中的燃烧副产物形成速率的方法和设备。 该燃烧器具有相关的火焰扫描器,其聚焦在燃烧器火焰的小面积上以获得火焰的图像信号。 从图像信号产生表示燃烧器的可见光谱中的时间燃烧特性的火焰信号。 燃烧器处的燃烧湍流由火焰信号通过与燃烧副产物值和燃烧器处不同燃烧副产物水平有关的动态不变量来分析,并且燃烧湍流与燃烧副产物形成率 该方法和设备也可用于将多个燃烧器处的燃烧湍流与相关的燃烧副产物形成速率相关联。