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61. 发明申请

WO2017074952A1 BIOFILMS, COMPONENTS AND METHODS OF USE TO REDUCE BIOFOULING AND CONTAMINATION 审中-公开
标题翻译：生物膜，组件和使用方法，以减少生物污染和污染
公开(公告)号：WO2017074952A1
公开(公告)日：2017-05-04
申请号：PCT/US2016/058701
申请日：2016-10-25
申请人： THE PENN STATE RESEARCH FOUNDATION
发明人： WOOD, Thomas K. , KUMAR, Manish , WOOD, Thammajun L.
IPC分类号： C12N1/00 , C12N1/04 , C12N15/70 , C12N15/74 , C12N15/78 , C12R1/01
CPC分类号： A01N63/02 , C07K14/21 , C07K14/245 , C12N1/20 , C12N9/0075 , C12N9/1029 , C12N9/14 , C12Y114/13039 , C12Y203/01184 , C12Y303/02003
摘要： Biofilms are provided which are capable of regulating their own thickness, reducing contamination and preventing biofouling. Constructs are introduced into bacteria that comprise nucleic acid molecules encoding an autoinducer synthase polypeptide, a transcriptional regulator and a biofilm dispersal protein. Nucleic acid molecules may also be introduced which encode a nitric oxide synthase, an epoxide hydrolase, or both. Biofilms of the bacteria may be used to reduce biofouling and contamination of a surface.
摘要翻译：提供了能够调节其自身厚度，减少污染和防止生物污损的生物膜。将构建体引入包含编码自诱导剂合酶多肽，转录调节剂和生物膜分散蛋白的核酸分子的细菌中。也可以引入编码一氧化氮合酶，环氧化物水解酶或两者的核酸分子。细菌的生物膜可用于减少生物污染和表面污染。

62. 发明申请

WO2013167784A1 MINITRANSPOSÓN MINIUIB Y USOS DERIVADOS 审中-公开
标题翻译： MINIUIB MINITRANSPOSON和衍生用途
公开(公告)号：WO2013167784A1
公开(公告)日：2013-11-14
申请号：PCT/ES2013/070294
申请日：2013-05-09
申请人： xUNIVERSITAT DE LES ILLES BALEARS
发明人： CHRISTIE OLEZA, Joseph Alexander , BRUNET GALMÉS, Isabel , LALUCAT JO, Jorge , NOGALES FERNÁNDEZ, Balbina , BOSCH ZARAGOZA, Rafael
IPC分类号： C12N15/74 , C12N15/78 , C12N15/73
CPC分类号： C12N15/1082 , C12N15/70 , C12N15/78
摘要： La presente invención se refiere a un minitransposón que comprende las repeticiones invertidas izquierda y derecha de ISPst9 , a su uso para la transferencia de un ácido nucleico o para la mutagénesis aleatoria. Así mismo, la presente invención serefiere a un método para la transposición de un ácido nucleico desde una bacteria dadora a una bacteria receptora que comprende introducir el ácido nucleico a transponer entre las repeticiones invertidas del minitransposón de la invención, introducir el minitransposón en la bacteria dadora y conjugar ambas bacterias dadora y receptora.
摘要翻译：本发明涉及包含ISPst9的左右反向重复序列的转座子，以及其用于转移核酸或用于随机诱变的用途。此外，本发明涉及将核酸从供体细菌转移到受体细菌的方法，包括将待转移的核酸引入本发明的转座子的反转重复序列之间，将小转座子引入供体细菌并使供体和受体细菌共轭。

63. 发明申请

WO2012012605A3 BACTERIAL MEDIATED DELIVERY OF NUCLEAR PROTEIN INTO PLURIPOTENT AND DIFFERENTIATED CELLS 审中-公开
标题翻译：细菌介导的将核蛋白输送到多种和不同细胞中
公开(公告)号：WO2012012605A3
公开(公告)日：2012-04-26
申请号：PCT/US2011044797
申请日：2011-07-21
申请人： UNIV FLORIDA RES , JIN SHOUGUANG , BICHSEL CANDACE
发明人： JIN SHOUGUANG , BICHSEL CANDACE
IPC分类号： C12N15/78 , C12N1/21 , C12N5/074 , C12N5/10 , C12R1/38
CPC分类号： C12R1/385 , C07K14/21 , C07K14/4702 , C12N5/0696 , C12N15/87 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605
摘要： A modified P. aeruginosa type III secretion system has been developed that efficiently delivers selected proteins into a host cell. In one example, a functional nuclear Cre Recombinase is injected into embryonic stem (ES) cells and can be used to induce pluripotent stem (iPS) cells. This method of in vitro lineage directed differentiation prevents insertional mutagenesis and provides a route to selected stem cell renewal and cell-based therapies.
摘要翻译：已经开发了经修饰的铜绿假单胞菌III型分泌系统，其有效地将选择的蛋白质递送到宿主细胞中。在一个实例中，功能核Cre重组酶被注射到胚胎干（ES）细胞中，并且可以用于诱导多能干（iPS）细胞。这种体外谱系定向分化的方法预防插入突变，并提供了选择的干细胞更新和基于细胞的疗法的途径。

64. 发明申请

WO2011132852A2 지방산 함량 개선을 위해 개발된 슈도모나스 에어루지노사 균주 및 제조방법 审中-公开
标题翻译：开发用于改善脂肪酸含量的PSEUDOMONAS AERUGINOSA菌株及其制备方法
公开(公告)号：WO2011132852A2
公开(公告)日：2011-10-27
申请号：PCT/KR2011/001554
申请日：2011-03-07
申请人： 서강대학교 산학협력단 , 이진원 , 이선희 , 전은영
发明人： 이진원 , 이선희 , 전은영
IPC分类号： C12N1/21 , C12N15/52 , C12N15/78 , C12P7/64 , C12R1/385
CPC分类号： C12N15/52 , C12N9/1029 , C12N9/16 , C12N9/93 , C12P7/6409 , C12P7/6463 , C12Y203/01039 , C12Y301/02014 , C12Y604/01002
摘要： 본 발명은 지방산 함량 개선을 위해 개발된 슈도모나스 에어루지노사 균주 및 제조방법에 관한 것으로, 보다 상세하게는 슈도모나스 에어루지노사( Pseudomonas aeruginosa )의 아세틸-CoA 카복실라제 카복실트랜스퍼라제 서브유닛 알파를 코딩하는 뉴클레오타이드 및/또는 말로닐-CoA-[아실운반단백질]트랜스아실라제트랜스아실라제를 코딩하는 뉴클레오타이드, 및/또는 스트렙토코커스 피오제네스( Streptococcus pyogenes )의 아실(아실운반단백질)티오에스터라제를 코딩하는 뉴클레오타이드를 포함하는 발현벡터로 형질전환된 슈도모나스 에어루지노사 및 그의 제조방법에 관한 것이다. 본 발명에 따른 형질전환된 슈도모나스 에어루지노사는 유전적 안정성이 높고 지질 또는 지방산 함량이 매우 높으므로, 지방산 생산을 대량 생산하는데 유용하게 사용될 수 있다.
摘要翻译：本发明涉及为提高脂肪酸含量而开发的铜绿假单胞菌菌株及其制造方法。更具体地说，本发明涉及转化成表达载体的铜绿假单胞菌，所述表达载体包括铜绿假单胞菌的编码乙酰辅酶A羧化酶羧基转移酶亚基α和/或核苷酸编码丙二酰-CoA- [酰基载体蛋白]转酰基甲酰四氢叶酸和/或化脓性链球菌的酰基（酰基载体蛋白）硫酯酶的核苷酸编码方法和铜绿假单胞菌的制造方法。由于根据本发明的转化的铜绿假单胞菌具有高的遗传稳定性和非常高含量的脂质或脂肪酸，因此本发明的转化的绿脓假单胞菌可用于大量生产脂肪酸。

65. 发明申请

WO2010008764A1 PSEUDOMONAS FLUORESCENS STRAINS FOR PRODUCTION OF EXTRACELLULAR RECOMBINANT PROTEIN 审中-公开
标题翻译：用于生产细胞外重组蛋白的PSEUDOMONAS FLUORESCENS菌株
公开(公告)号：WO2010008764A1
公开(公告)日：2010-01-21
申请号：PCT/US2009/047918
申请日：2009-06-19
申请人： DOW GLOBAL TECHNOLOGIES INC. , RETTALACK, Diane, M. , CHEW, Lawrence, C.
发明人： RETTALACK, Diane, M. , CHEW, Lawrence, C.
IPC分类号： C12N15/78 , C12P21/02
CPC分类号： C12R1/39 , C12N15/78 , C12P21/02
摘要： Provided herein are compositions and methods for improving secretion of heterologous protein from a population of Pseudomonas fluorescens-derived cells. The compositions include host cell populations that have been genetically modified to increase secretion into the extracellular space of a heterologous protein of interest. The genetically-modified host cell populations are useful for improving the extracellular secretion of any protein of interest, including therapeutic proteins, hormones, a growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like. In various embodiments, the modified P. fluorescens host cell populations include one or more genomic mutations responsible for or contributing to the improved level of extracellular secretion. The host cell populations of the invention include the modified P. fluorescens strains deposited in the American Type Tissue Culture Collection and assigned accession numbers PTA-8981 and PTA-8982.
摘要翻译：本文提供了用于改善来自荧光假单胞菌衍生细胞群的异源蛋白质分泌的组合物和方法。组合物包括已被遗传修饰以增加分泌到感兴趣的异源蛋白质的细胞外空间中的宿主细胞群体。遗传修饰的宿主细胞群体可用于改善任何目标蛋白质的细胞外分泌，包括治疗性蛋白质，激素，生长因子，细胞外受体或配体，蛋白酶，激酶，血液蛋白，趋化因子，细胞因子，抗体等。在各种实施方案中，经修饰的荧光假单胞菌宿主细胞群体包括负责或有助于提高细胞外分泌水平的一个或多个基因组突变。本发明的宿主细胞群包括保藏在美国组织培养物保藏中心的经修饰的荧光假单胞菌菌株，并分配了登录号PTA-8981和PTA-8982。

66. 发明申请

WO2010002966A2 HIGH THROUGHPUT SCREENING METHOD AND USE THEREOF TO IDENTIFY A PRODUCTION PLATFORM FOR A MULTIFUNCTIONAL BINDING PROTEIN 审中-公开
标题翻译：高通量筛选方法及其用于鉴定用于多功能结合蛋白的生产平台
公开(公告)号：WO2010002966A2
公开(公告)日：2010-01-07
申请号：PCT/US2009049366
申请日：2009-07-01
申请人： DOW GLOBAL TECHNOLOGIES INC , RETALLACK DIANE
发明人： RETALLACK DIANE
IPC分类号： C12N15/62 , C12N15/78 , G01N33/50
CPC分类号： C12N15/1037 , C07K16/005 , C07K16/40 , C40B40/02
摘要： Methods of identifying and expressing an antibody variant are disclosed wherein the method comprises identifying a binding region in an antibody, fusing the binding region to a plurality of scaffolds of antibody constant regions to obtain antibody fragment variants, expressing the antibody fragment variants in organisms to form constructs and expressing the constructs carried by the organisms to form induced cultures, wherein the organisms are expressed in HTP mode.
摘要翻译：公开了鉴定和表达抗体变体的方法，其中该方法包括鉴定抗体中的结合区，将结合区与多个抗体恒定区的支架融合以获得抗体片段变体，在生物体中表达抗体片段变体以形成构建并表达由生物体携带的构建体以形成诱导培养物，其中所述生物体以HTP模式表达。

67. 发明申请

WO2006037215A1 EXPRESSION SYSTEM, COMPONENTS THEREOF AND METHODS OF USE 审中-公开
标题翻译：表达系统，组件及其使用方法
公开(公告)号：WO2006037215A1
公开(公告)日：2006-04-13
申请号：PCT/CA2005/001508
申请日：2005-10-03
申请人： NATIONAL RESEARCH COUNCIL OF CANADA , YU, Yan , MULLICK, Alaka , MASSIE, Bernard
发明人： YU, Yan , MULLICK, Alaka , MASSIE, Bernard
IPC分类号： C12N15/78 , C12N15/31 , C07K14/21 , C12P21/00 , C12N5/10 , C12N15/63
CPC分类号： C07K14/21
摘要： Recently, the development of inducible expression systems has involved exploitation of the p-cym operon from Pseudomonas putida . Disclosed herein are novel expression systems and components thereof, which involve the development of CymR variants with reverse DNA binding activity, such that they exhibit increased affinity for DNA in a presence rather than an absence of an effector molecule such as cumene or an equivalent thereof. Also disclosed are the CymR variants, fusion proteins incorporating such variants, and their use in the control and expression of polynucleotides.
摘要翻译：最近，诱导型表达系统的发展涉及从恶臭假单胞菌（Pseudomonas putida）中开发p-cym操纵子。本文公开了新的表达系统及其组分，其涉及开发具有反向DNA结合活性的CymR变体，使得它们在存在而不是不存在效应分子例如枯烯或其等同物时表现出对DNA的增加的亲和力。还公开了CymR变体，掺入这些变体的融合蛋白及其在多核苷酸的控制和表达中的用途。

68. 发明申请

WO2005069913A3 EXPRESSION OF MAMMALIAN PROTEINS IN PSEUDOMONAS FLUORESCENS 审中-公开
标题翻译：在PSEUDOMONAS FLRORESCENS中表达MAMMALIAN蛋白
公开(公告)号：WO2005069913A3
公开(公告)日：2005-09-22
申请号：PCT/US2005001549
申请日：2005-01-18
申请人： DOW GLOBAL TECHNOLOGIES INC , RETALLACK DIANE M , SQUIRES CHARLES H , WATKINS DAVID C , GAERTNER FRANK H , LEE STACEY LYNN , SHUTTER ROBERT
发明人： RETALLACK DIANE M , SQUIRES CHARLES H , WATKINS DAVID C , GAERTNER FRANK H , LEE STACEY LYNN , SHUTTER ROBERT
IPC分类号： C07K14/57 , C07K14/61 , C12N1/21 , C12N15/10 , C12N15/63 , C12N15/64 , C12N15/67 , C12N15/74 , C12N15/78
CPC分类号： C07K14/61 , C07K14/57 , C12N15/78
摘要： The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances. Processes for improved production of isolated mammalian, particularly human, proteins are provided.
摘要翻译：本发明是通过在假单胞菌，特别是荧光假单胞菌生物体中表达来改进重组哺乳动物蛋白质生产的方法。该方法在可比较的情况下，通过已知的表达系统如大肠杆菌改善哺乳动物蛋白质，特别是人源或人源蛋白质的产生。提供了用于改进分离的哺乳动物，特别是人类蛋白质生产的方法。

69. 发明申请

WO2004033633A3 COMPATIBLE HOST/VECTOR SYSTEMS FOR EXPRESSION OF DNA 审中-公开
标题翻译：用于表达DNA的相容性主机/载体系统
公开(公告)号：WO2004033633A3
公开(公告)日：2004-12-23
申请号：PCT/US0331383
申请日：2003-10-03
申请人： EMBIOSIS PHARMACEUTICALS , HOCH JAMES A , DARTOIS VERONIQUE
发明人： HOCH JAMES A , DARTOIS VERONIQUE
IPC分类号： C12N15/74 , C12N15/75 , C12N15/63 , C12N15/76 , C12N15/77 , C12N15/78
CPC分类号： C12N15/75 , C12N15/74 , C12N15/746
摘要： The present invention provides shuttle vectors compatible with a panel of different bacterial hosts and capable of replicating in evolutionarily divergent bacterial species. In various embodiments the shuttle vectors of the present invention can integrate into the genomes of and/or replicate in both Gram-negative and Gram-positive organisms of both high and low GC content. The vectors of the present invention therefore make possible the expression of environmental DNA in varied families of bacterial hosts using a single vector that is transferable between hosts by a variety of methods. In some embodiments, the vector can replicate in a genera of bacteria from each of the following groups: Escherichia or Klebsiella; Pseudomonas, Xanthomonas, or Acetobacter; Bacillus or Clostridia, and Corynebacteria, Streptomycete, or Rhodococcus. In various embodiments, the vectors are cosmid, plasmids, fosmids, or bacterial artificial chromosomes (BACs).
摘要翻译：本发明提供了与一组不同细菌宿主相容并能够在进化上不同的细菌物种中复制的穿梭载体。在各种实施方案中，本发明的穿梭载体可以整合到高GC含量和低GC含量的革兰氏阴性和革兰氏阳性生物体的基因组中和/或复制。因此，本发明的载体可以使用可通过各种方法在宿主之间转移的单一载体在不同的细菌宿主家族中表达环境DNA。在一些实施方案中，载体可以在来自以下各组的细菌属中复制：埃希氏菌属或克雷伯菌属; 假单胞菌属，黄单胞菌属或醋杆菌属; 芽孢杆菌属或梭菌属，以及棒状杆菌属，链霉菌属或红球菌属。在各种实施方案中，载体是粘粒，质粒，fosmids或细菌人工染色体（BAC）。

70. 发明申请

WO0214551B1 BIOLUMINESCENT METHODS FOR DIRECT VISUAL DETECTION OF ENVIRONMENTAL COMPOUNDS 审中-公开
公开(公告)号：WO0214551B1
公开(公告)日：2003-06-26
申请号：PCT/US0125014
申请日：2001-08-10
申请人： UNIV TENNESSEE RES CORP , SANSEVERINO JOHN , SAYLER GARY S , RIPP STEVEN A
发明人： SANSEVERINO JOHN , SAYLER GARY S , RIPP STEVEN A
IPC分类号： C12N1/21 , C12N15/63 , C12N15/78 , C12Q1/02 , C12Q1/66 , C12N11/02
CPC分类号： C12Q1/025 , C12N15/635 , C12N15/78
摘要： The invention relates to devices and methods that utilize immobilized bacterial bioreporters genetically engineered to emit light visible to the naked eye in the presence of selected analytes. An exemplary bioreporter is an E. coli that has been modified to respond to mercury II as a result of incorporation of a merRop/Iux gene cassette into its genome. Systems employing analagously engineered microorganisms can detect selected toxins quickly without need for expensive instruments or highly trained technicians.

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