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51. 发明申请

WO2007099231A8 SYSTEM FOR THE EXPRESSION OF A GENE OF INTEREST IN YEAST 审中-公开
标题翻译：用于表达YEAST中利益基因的系统
公开(公告)号：WO2007099231A8
公开(公告)日：2009-07-16
申请号：PCT/FR2007000365
申请日：2007-02-28
申请人： MANE FILS V , UNIV VICTOR SEGALEN BORDEAUX 2 , CENTRE NAT RECH SCIENT , AIGLE MICHEL , NESS FREDERIQUE , ZUCCA JOSEPH , LAMBERT FANNY , MANE JEAN
发明人： AIGLE MICHEL , NESS FREDERIQUE , ZUCCA JOSEPH , LAMBERT FANNY , MANE JEAN
IPC分类号： C12N15/90 , C12N15/81
CPC分类号： C12N15/905 , C12N15/815
摘要： The invention relates to a system for the expression of a gene of interest in a cell, preferably a yeast, comprising: (1) means for integrating the system into the genome of said cell, comprising two nucelotide sequences, (2) means for selecting said cell in which the system is integrated, comprising a selection insert with two LoxP sequences flanking a promoter, an antibiotic resistance gene selection marker and a terminator, and (3) an expression cassette of the gene of interest, comprising a promoter enabling the expression of said gene, at least one cloning site enabling the integration of the gene, and a terminator.
摘要翻译：本发明涉及用于在细胞，优选酵母中表达感兴趣的基因的系统，其包括：（1）将所述系统整合到所述细胞的基因组中的装置，其包含两个核苷酸序列，（2）用于选择的装置所述细胞集成在其中，包含选择插入物，其具有两个位于启动子侧翼的LoxP序列，抗生素抗性基因选择标记和终止子，以及（3）感兴趣的基因的表达盒，其包含能够表达的所述基因，至少一个能够整合基因的克隆位点和终止子。

52. 发明申请

WO2007136865A2 RECOMBINANT VECTORS 审中-公开
标题翻译：重组载体
公开(公告)号：WO2007136865A2
公开(公告)日：2007-11-29
申请号：PCT/US2007012198
申请日：2007-05-18
申请人： GLYCOFI INC , NETT JUERGEN , DAVIDSON ROBERT
发明人： NETT JUERGEN , DAVIDSON ROBERT
IPC分类号： C12N15/74
CPC分类号： C12N15/815 , C12N15/905
摘要： Methods and materials are provided for integrating heterologous nucleic acids into the genome of a cell or virus without disrupting expression of genes adjacent to the insertion site.
摘要翻译：提供的方法和材料用于将异源核酸整合到细胞或病毒的基因组中，而不会破坏与插入位点相邻的基因的表达。

53. 发明申请

WO2004104165A2 URA5 GENE AND METHODS FOR STABLE GENETIC INTEGRATION IN YEAST 审中-公开
标题翻译： URA5基因和方法用于稳定的遗传整合在YEAST
公开(公告)号：WO2004104165A2
公开(公告)日：2004-12-02
申请号：PCT/US2004/013488
申请日：2004-04-30
申请人： GLYCOFI, INC. , NETT, Juergen, H.
发明人： NETT, Juergen, H.
IPC分类号： C12N
CPC分类号： C12N15/905 , C07H21/04 , C07K2319/00 , C12N9/1077 , C12N15/81 , C12N15/815
摘要： A novel gene encoding P. pastoris orotate-phosphoribosyl transferase (URA5) is disclosed. Methods for producing and selecting yeast strains capable of stable genetic integration of heterologous sequences into the host genome are also provided.
摘要翻译：公开了编码巴斯德毕赤酵母 - 磷酸核糖转移酶（URA5）的新型基因。还提供了用于产生和选择能够将异源序列稳定遗传整合到宿主基因组中的酵母菌株的方法。

54. 发明申请

WO2003062435A1 A METHOD FOR GENERATING ENGINEERED CELLS FOR LOCUS SPECIFIC GENE REGULATION AND ANALYSIS 审中-公开
标题翻译：用于产生用于位置特异性基因调控和分析的工程细胞的方法
公开(公告)号：WO2003062435A1
公开(公告)日：2003-07-31
申请号：PCT/US2003/001361
申请日：2003-01-17
申请人： MORPHOTEK, INC. , GRASSO, Luigi , KLINE, J., Bradford , NICOLAIDES, Nicholas, C. , SASS, Philip, M.
发明人： GRASSO, Luigi , KLINE, J., Bradford , NICOLAIDES, Nicholas, C. , SASS, Philip, M.
IPC分类号： C12N15/87
CPC分类号： C12N15/905 , C12N15/102 , C12N15/902 , C12N15/907
摘要： Inhibitors of mismatch repair can be used to generate hypermutable cells and organisms. By inhibiting this process in cells, new cell lines and varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of homologous recombination. These methods are useful for generating targeted loci that can alter the expression profiles of target genes as well as tag exons of gene with a reporter marker to facilitate the monitoring of a given gene product when the host is grown under different conditions or exposed to biological and chemical entities.
摘要翻译：不匹配修复的抑制剂可用于产生超可变细胞和生物体。通过在细胞中抑制该过程，可以比通过依赖于同源重组的天然速率更有效地制备具有新颖和有用性质的新细胞系和品种。这些方法可用于产生靶基因座，其可以改变目标基因的表达谱以及基因的标签外显子与报道标记，以便当宿主在不同条件下生长或暴露于生物和化学实体。

55. 发明申请

WO02022811A2 MODULATION OF MEIOTIC RECOMBINATION 审中-公开
标题翻译：调节重建
公开(公告)号：WO02022811A2
公开(公告)日：2002-03-21
申请号：PCT/CA2001/001306
申请日：2001-09-12
IPC分类号： C12N15/82 , C12N15/873 , C12N15/90 , C12N15/01 , A01H1/02 , A01K67/02 , C12Q1/68
CPC分类号： C12N15/81 , C12N15/8213 , C12N15/873 , C12N15/905 , C12N15/907
摘要： The invention provides methods of modifying the level of expression or functional activity of factors such as enzymes or other catalytic proteins or structural proteins, alone or in concert, to modify the frequency of meiotic homologous recombination involving the exchange of genetic information between non-sister chromatids from homologous maternal and paternal chromosomes. The steps at which modulation may occur include: homologous chromosome pairing, double-strand break formation; resection; strand invasion; branch migration; and resolution. Methods of plant and animal breeding are also provided that utilize the modulation of meiotic homologous recombination.
摘要翻译：本发明提供了单独或一致地修饰诸如酶或其他催化蛋白或结构蛋白等因素的表达水平或功能活性的方法，以修饰涉及非姐妹染色单体之间的遗传信息交换的减数分裂同源重组的频率来自同源的母体和父系染色体。可能发生调节的步骤包括：同源染色体配对，双链断裂形成; 切除; 线入侵; 分支迁移; 和决议。还提供了利用减数分裂同源重组调控的植物和动物育种方法。

56. 发明申请

WO00055311A2 GENE MODIFICATION BY HOMOLOGOUS RECOMBINATION 审中-公开
标题翻译：基因改造通过同源重建
公开(公告)号：WO00055311A2
公开(公告)日：2000-09-21
申请号：PCT/US2000/006959
申请日：2000-03-16
IPC分类号： C12N15/90 , C12N15/00 , C12N15/11 , C12N15/63 , C12N15/65 , C12N15/81
CPC分类号： C12N15/905
摘要： A method for the modification of a specific gene of interest is described. The method employs two genetic constructs. One construct is a modification plasmid comprising the target gene or nucleic acid fragment of interest. The second construct is a modification cassette comprising yeast selectable markers, and optionally other DNA, bounded by flanking DNA which is complementary to portions of the target gene residing on the modification plasmid. Both constructs are transformed into budding yeast where homologous recombination occurs between the cassette and the plasmid. The resulting altered modification plasmid contains an insertion in the target gene in the regions governed by the sequence of the cassette flanking DNA.
摘要翻译：描述了用于修饰目标特定基因的方法。该方法采用两种遗传构建体。一种构建体是包含靶基因或目的核酸片段的修饰质粒。第二种构建体是包含酵母选择性标记和任选的其它DNA的修饰盒，所述DNA可以通过与位于修饰质粒上的目标基因的部分互补的侧翼DNA结合。将两种构建体转化入芽殖酵母中，其中盒和质粒之间发生同源重组。所产生的改变的修饰质粒在由侧翼DNA侧翼的序列控制的区域中包含靶基因中的插入物。

57. 发明申请

WO98037208A1 EXPRESSION OF HETEROLOGOUS PROTEINS 审中-公开
标题翻译：异位蛋白的表达
公开(公告)号：WO98037208A1
公开(公告)日：1998-08-27
申请号：PCT/IB1998/000269
申请日：1998-02-18
IPC分类号： C12N15/09 , C07K14/18 , C07K14/475 , C07K14/52 , C12N1/19 , C12N1/21 , C12N9/02 , C12N9/90 , C12N15/90 , C12P21/02 , C12N15/63 , C12N5/10
CPC分类号： C07K14/005 , C07K14/52 , C12N9/0036 , C12N9/90 , C12N15/905 , C12N2770/36122 , C12P21/02 , C12Y108/01008 , C12Y503/04001
摘要： An expression system which provides heterologous proteins expressed by a non-native host organism but which have native-protein-like biological activity and/or structure. Disclosed are vectors, expression hosts and methods for expressing the heterologous proteins. The expression system involves co-expression of protein factor(s) which is/are capable of catalyzing disulphide bond formation and desired heterologous protein(s). The expression system is presented using yeast cells as the preferred host, protein disulphide isomerase (PDI) and thioredoxin (TRX) as the preferred examples of the protein factors and HCV-E2715 envelope glycoprotein and human FIGF as the preferred examples of the heterologous proteins.
摘要翻译：提供由非天然宿主生物表达但具有天然蛋白样生物活性和/或结构的异源蛋白质的表达系统。公开了用于表达异源蛋白的载体，表达宿主和方法。表达系统涉及能够催化二硫键形成和所需异源蛋白质的蛋白质因子的共表达。使用酵母细胞作为优选宿主，蛋白质二硫键异构酶（PDI）和硫氧还蛋白（TRX）作为蛋白质因子和HCV-E2715包膜糖蛋白和人FIGF的优选实例作为异源蛋白质的优选实施例提供表达系统。

58. 发明申请

WO1998007873A1 YEAST VECTORS AND PROCESS FOR PRODUCING PROTEINS WITH THE USE OF THE SAME 审中-公开
标题翻译： YEAST VECTORS和使用该蛋白的蛋白质生产过程
公开(公告)号：WO1998007873A1
公开(公告)日：1998-02-26
申请号：PCT/JP1997002924
申请日：1997-08-22
申请人： KIRIN BEER KABUSHIKI KAISHA , KONDO, Keiji , MIURA, Yutaka
发明人： KIRIN BEER KABUSHIKI KAISHA
IPC分类号： C12N15/68
CPC分类号： C12N9/2411 , C07K14/43 , C12N9/2408 , C12N15/815 , C12N15/905 , C12Y302/01001
摘要： Vectors which are to be integrated onto yeast chromosomes by multi-copying; and vectors which are to be integrated onto yeast chromosomes by multi-copying and show improved stability of expression units on the chromosomes. These vectors are ones each containing a marker gene for selecting a transformant, a shortened promoter sequence linked to the marker gene to thereby serve as an operator, and a sequence homologous with the chromosomal DNA of Candida utilis, optionally together with a heterogenous gene or a gene originating in C. utilis. It is linearized by cleaving with restriction enzymes within the homologous DNA sequence or at both ends of the same and, thus, enables the integration of the heterogenous gene or the gene originating in C. utilis onto the chromosomal DNA of C. utilis via homologous recombination.
摘要翻译：通过多重复制整合到酵母染色体上的载体; 以及通过多次复制整合到酵母染色体上的载体，并且显示改善染色体上表达单元的稳定性。这些载体是含有用于选择转化体的标记基因，与标记基因连接的缩短的启动子序列，从而用作操纵子的序列，以及与产朊假丝酵母的染色体DNA同源的序列，任选与异源基因或源自产朊酵母的基因。通过用同源DNA序列内的限制性内切酶或同源DNA两端的限制性内切酶线性化，从而通过同源重组使异源基因或源自产朊酵母的基因与产朊假单胞菌的染色体DNA整合。

59. 发明申请

WO1991015588A1 RECOMBINANT YEASTS CONTAINING THE DNA SEQUENCES CODING FOR XYLOSE REDUCTASE- AND XYLITOL DEHYDROGENASE ENZYMES 审中-公开
标题翻译：含有XYLOSE还原酶和XYLITOL DEHYDROGENASE酶的DNA序列编码的重组子
公开(公告)号：WO1991015588A1
公开(公告)日：1991-10-17
申请号：PCT/FI1991000103
申请日：1991-04-08
申请人： VALTION TEKNILLINEN TUTKIMUSKESKUS , HALLBORN, Johan , PENTTILÄ, Merja , OJAMO, Heikki , WALFRIDSSON, Mats , Airaksinen, Ulla , KERÄNEN, Sirkka , HAHN-HÄGERDAL, Bärbel
发明人： VALTION TEKNILLINEN TUTKIMUSKESKUS
IPC分类号： C12N15/53
CPC分类号： C12N9/0006 , C12N15/52 , C12N15/81 , C12N15/905 , C12P7/06 , C12P7/18 , C12P7/40 , C12P19/02 , Y02E50/17 , Y02P20/582
摘要： This invention relates to recombinant-DNA-technology. Specifically this invention relates to new recombinant yeast strains transformed with xylose reductase and/or xylitol dehydrogenase enzyme genes. A yeast strain transformed with the xylose reductase gene is capable of reducing xylose to xylitol and consequently of producing xylitol in vivo. If both of these genes are transformed into a yeast strain, the resultant strain is capable of producing ethanol on xylose containing medium during fermentation. Further, the said new yeast strains are capable of expressing the said two enzymes. Xylose reductase produced by these strains can be used in an enzymatic process for the production of xylitol in vitro.
摘要翻译：本发明涉及重组DNA技术。具体地说，本发明涉及用木糖还原酶和/或木糖醇脱氢酶基因转化的新的重组酵母菌株。用木糖还原酶基因转化的酵母菌株能够将木糖还原成木糖醇，从而在体内产生木糖醇。如果将这两个基因转化成酵母菌株，则所得菌株能够在发酵期间在含有木糖的培养基上产生乙醇。此外，所述新酵母菌株能够表达所述两种酶。由这些菌株产生的木糖还原酶可用于体外生产木糖醇的酶法。

60. 发明申请

WO2018050666A1 GENOMIC INTEGRATION OF DNA FRAGMENTS IN FUNGAL HOST CELLS 审中-公开
标题翻译：基因组DNA片段在真菌宿主细胞中的基因整合
公开(公告)号：WO2018050666A1
公开(公告)日：2018-03-22
申请号：PCT/EP2017/072967
申请日：2017-09-13
申请人： NOVOZYMES A/S
发明人： KLITGAARD, Dorte, M.K.
IPC分类号： C12N15/90
CPC分类号： C12N15/902 , C07K14/37 , C07K2319/02 , C07K2319/036 , C12N9/0036 , C12N9/22 , C12N15/65 , C12N15/80 , C12N15/905
摘要： The instant invention relates to methods for the in vivo assembly and integration of a polynucleotide of interest at a specific chromosomal target site in a fungal host cell, said method comprising transforming the host cell with: iv. at least one polynucleotide fragment comprising an upstream flanking region and a 5' part of the polynucleotide of interest in that order; v. at least one polynucleotide fragment comprising a 3' part of the polynucleotide of interest and a downstream flanking region in that order; and, optionally vi. one or more additional polynucleotide fragments each comprising a 5' part and a 3' part of the polynucleotide of interest; wherein the 5' part and 3' part of each polynucleotide fragment overlap with that or those of another fragment with at least 20 bp, whereby the fragments cover the entire polynucleotide of interest, and wherein the flanking regions are of sufficient size and homology to effectuate homologous recombination with the specific target site for chromosomal integration.
摘要翻译：本发明涉及用于在特定染色体靶位点在真菌宿主细胞中体内组装和整合感兴趣多核苷酸的方法，所述方法包括用以下方法转化宿主细胞：iv。至少一个多核苷酸片段，其按顺序包含上游侧翼区和5'部分目的多核苷酸; v。至少一个多核苷酸片段，所述多核苷酸片段按顺序包含目的多核苷酸的3'部分和下游侧翼区域; 并且可选地vi。一个或多个另外的多核苷酸片段，每个包含目的多核苷酸的5'部分和3'部分; 其中每个多核苷酸片段的5'部分和3'部分与具有至少20bp的另一个片段的5'部分和3'部分重叠，由此片段覆盖整个目的多核苷酸，并且其中侧翼区域具有足够的大小和同源性以实现与染色体整合的特定靶位点同源重组。

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