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31. 发明申请

WO2014004609A3 RAPID ASSAYS FOR T-CELL ACTIVATION BY RNA MEASUREMENTS USING FLOW CYTOMETRY 审中-公开
标题翻译：利用流式细胞术测量RNA测量对T细胞活化的快速测定
公开(公告)号：WO2014004609A3
公开(公告)日：2014-02-27
申请号：PCT/US2013047774
申请日：2013-06-26
申请人： UNIV NEW JERSEY MED
发明人： BUSHKIN YURI , GENNARO MARIA LAURA , TYAGI SANJAY , PINE RICHARD
IPC分类号： C12Q1/68 , C07H21/04 , G01N33/00
CPC分类号： C12Q1/6841 , C12Q2565/626
摘要： The present invention relates to a method for rapidly detecting copies of at least one RNA molecule expressed in individual cells and uses thereof.
摘要翻译：本发明涉及用于快速检测在个体细胞中表达的至少一种RNA分子的拷贝的方法及其用途。

32. 发明申请

WO2012078321A1 PHASED GENOME SEQUENCING 审中-公开
标题翻译：相关基因测序
公开(公告)号：WO2012078321A1
公开(公告)日：2012-06-14
申请号：PCT/US2011/060832
申请日：2011-11-15
申请人： THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY , WONG, Wing H. , YANG, Hong
发明人： WONG, Wing H. , YANG, Hong
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6869 , C12N15/1065 , C12Q1/6804 , C12Q1/6874 , C12Q2537/164 , C12Q2565/626
摘要： Disclosed are methods for determining phased nucleic acid sequence for a single chromosome of interest and/or a single chromosomal fragment of interest. Also provided are methods for determining phased nucleic acid sequences for a plurality of single chromosomes of interest and/or a plurality of single chromosomal fragments of interest. The plurality of single chromosomes of interest may be of one chromosome type or of two or more chromosome types. Also provided is a method for isolating a plurality of chromosomal fragments of a specified size range, where the chromosomal fragments are from one or more specified regions of the genome, wherein the plurality of chromosomal fragments may be sequenced together to provide unphased nucleic acid sequence for the chromosomal fragments.
摘要翻译：公开了用于确定感兴趣的单个染色体和/或感兴趣的单个染色体片段的相序核酸序列的方法。还提供了用于确定感兴趣的多个单个染色体和/或感兴趣的多个单个染色体片段的相序核酸序列的方法。多个感兴趣的单个染色体可以是一种染色体类型或两种或更多种染色体类型。还提供了一种用于分离特定大小范围的多个染色体片段的方法，其中所述染色体片段来自所述基因组的一个或多个特定区域，其中所述多个染色体片段可以一起测序以提供未定型的核酸序列染色体片段。

33. 发明申请

WO2011156432A2 SCANNING MULTIFUNCTIONAL PARTICLES 审中-公开
标题翻译：扫描多功能颗粒
公开(公告)号：WO2011156432A2
公开(公告)日：2011-12-15
申请号：PCT/US2011/039529
申请日：2011-06-07
申请人： FIREFLY BIOWORKS, INC. , PREGIBON, Daniel, C. , MARINI, Davide, M. , STONER, Isaac , WINDEMUTH, Andreas , ERPS, Timothy
发明人： PREGIBON, Daniel, C. , MARINI, Davide, M. , STONER, Isaac , WINDEMUTH, Andreas , ERPS, Timothy
IPC分类号： G01N33/483 , G01N15/14 , C12Q1/68 , C12Q1/02 , G01N21/47
CPC分类号： C12Q1/6825 , C12Q1/6809 , C12Q1/6813 , C12Q1/6816 , C12Q1/6834 , C12Q1/6888 , C12Q1/689 , C12Q1/6895 , C12Q1/70 , G01N15/14 , G01N21/47 , G01N21/6428 , G01N33/6803 , C12Q2565/626 , C12Q2525/161 , C12Q2525/191 , C12Q2533/107
摘要： The present invention provides, among other things, methods and systems for characterizing multifunctional objects using a flow-through device, such as, a flow cytometer. In some embodiments, an inventive method according to the present invention includes one more steps of (a) interrogating a plurality of objects (e.g., particles), wherein each individual object (e.g., particle) comprises one or more interrogation regions detectable as a sequence of events; (b) recording multiple events, wherein each individual event corresponds to each individual interrogation region detectable above a pre-determined triggering threshold; (c) grouping the recorded multiple events, and (d) characterizing the plurality of objects based on the grouped events.
摘要翻译：本发明尤其提供了使用流通装置如流式细胞仪来表征多功能物体的方法和系统。在一些实施例中，根据本发明的发明方法包括以下步骤：（a）询问多个对象（例如，粒子），其中每个单个对象（例如，粒子）包括可被检测为序列的一个或多个询问区域的事件; （b）记录多个事件，其中每个单独的事件对应于在预先确定的触发阈值之上可检测的每个个别询问区域; （c）对记录的多个事件进行分组，以及（d）基于分组的事件来表征多个对象。

34. 发明申请

WO2008029295A3 A METHOD FOR DETECTING NUCLEIC ACIDS 审中-公开
标题翻译：一种检测核酸的方法
公开(公告)号：WO2008029295A3
公开(公告)日：2011-05-12
申请号：PCT/IB2007003732
申请日：2007-06-29
申请人： ROSETTA GENOMICS LTD , SHLOMIT GILAD , MEIRI ESTHER , YERUSHALMI NOGA
发明人： SHLOMIT GILAD , MEIRI ESTHER , YERUSHALMI NOGA
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6816 , C12Q1/6832 , C12Q1/6853 , C12Q1/6886 , C12Q2600/136 , C12Q2600/178 , C12Q2545/101 , C12Q2563/149 , C12Q2565/501 , C12Q2565/626 , C12Q2521/107 , C12Q2525/207 , C12Q2525/301 , C12Q2561/101 , C12Q2525/161
摘要： Described herein are methods and compositions for detecting, amplifying and labeling targeted nucleic acids, including microRNAs.
摘要翻译：本文描述了用于检测，扩增和标记靶核酸，包括微小RNA的方法和组合物。

35. 发明申请

WO2009073345A3 ASSAY FOR DETECTING GENETIC ABNORMALITIES IN GENOMIC NUCLEIC ACIDS 审中-公开
标题翻译：检测基因组核酸遗传异常的检测方法
公开(公告)号：WO2009073345A3
公开(公告)日：2009-07-30
申请号：PCT/US2008083673
申请日：2008-11-14
申请人： QUEST DIAGNOSTICS INVEST INC , ALBITAR MAHER
发明人： ALBITAR MAHER
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , Y10T436/143333 , C12Q2565/518 , C12Q2565/626
摘要： The present invention provides methods of detecting unamplified genomic nucleic acid anchored to a solid support. The methods are useful for the detecting genetic abnormalities associated with various diseases, diagnosis, and prognosis.
摘要翻译：本发明提供了检测锚定到固体支持物上的未扩增的基因组核酸的方法。该方法可用于检测与各种疾病，诊断和预后有关的遗传异常。

36. 发明申请

WO2006113590A2 FLOW-CYTOMETRIC HETERODUPLEX ANALYSIS FOR DETECTION OF GENETIC ALTERATIONS 审中-公开
标题翻译：用于检测遗传变异的流式细胞异位异构体分析
公开(公告)号：WO2006113590A2
公开(公告)日：2006-10-26
申请号：PCT/US2006014346
申请日：2006-04-17
申请人： CEDARS SINAI MEDICAL CENTER , SZABO GABOR , LUSTYIK GYOERGY
发明人： SZABO GABOR , LUSTYIK GYOERGY
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2565/626 , C12Q2545/114 , C12Q2523/107
摘要： The present invention provides methods and kits useful for the detection of genetic alterations and to diagnose a disease condition caused by a genetic alteration. The invention involves two different PCR products, one control "unaltered" product and one sample product, which may contain "altered" DNA, that are mixed, denatured, and allowed to re-anneal. The DNA duplexes are then treated enzymatically or chemically to cleave single stranded or mismatched DNA regions resulting from the genetic alterations. The alterations are then quantitated by measuring a decrease in a fluorescent signal resulting from removal of the fluorescent tag. Further embodiments of the invention include a second, different fluorescent tag that is used to measure non-specific degradation of the duplexes. Other embodiments of the invention include the use of avidin coated microbeads to which the duplexes may be attached for quantitation by flow cytometry. Still further embodiments include biological microbeads for this purpose.
摘要翻译：本发明提供了可用于检测遗传改变并诊断由遗传改变引起的疾病状况的方法和试剂盒。本发明涉及两种不同的PCR产物，一种对照“未改变”的产物和一种样品，其可以含有“改变的”DNA，其被混合，变性并允许再退火。然后将DNA双链体酶或化学处理以切割由遗传改变引起的单链或错配的DNA区域。然后通过测量由去除荧光标签产生的荧光信号的减少来定量改变。本发明的其它实施方案包括用于测量双链体的非特异性降解的第二种不同的荧光标签。本发明的其它实施方案包括使用亲和素包被的微珠，通过流式细胞术可将双链体连接到其上进行定量。另外的实施方案包括用于此目的的生物微珠。

37. 发明申请

WO2005095653A2 METHOD FOR COLLECTING PURIFIED CELLS 审中-公开
标题翻译：收集纯化细胞的方法
公开(公告)号：WO2005095653A2
公开(公告)日：2005-10-13
申请号：PCT/US2005/010713
申请日：2005-03-30
申请人： HEMATOLOGICS, INC. , LOKEN, Michael, R.
发明人： LOKEN, Michael, R.
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12Q1/6806 , C12Q2565/626 , C12Q2565/625
摘要： The present invention provides improved methods for collecting cells for subsequent analysis. More specifically, the invention provides methods for collecting purified cell samples from a flow cytometer onto a solid support on which cell lysis occurs and genetic material is immobilized.
摘要翻译：本发明提供了用于收集细胞以供随后分析的改进方法。更具体地说，本发明提供了从流式细胞仪收集纯化细胞样品到固体支持物上的方法，在固体支持物上发生细胞裂解并固定遗传物质。

38. 发明申请

WO2004108951A1 METHODS FOR ASSESSING THE INVASIVE POTENTIAL OF A CELL EMPLOYING CHROMATIN ANALYSIS 审中-公开
标题翻译：评估使用色胺分析的细胞的入侵潜能的方法
公开(公告)号：WO2004108951A1
公开(公告)日：2004-12-16
申请号：PCT/US2004/017859
申请日：2004-06-07
申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS , MANIOTIS, Andrew , FOLBERG, Robert
发明人： MANIOTIS, Andrew , FOLBERG, Robert
IPC分类号： C12Q1/00
CPC分类号： C12Q1/68 , C12Q1/6841 , C12Q2565/626 , C12Q2563/173 , C12Q2521/301
摘要： Embodiments of the invention relate to compositions and methods for evaluating or estimating the invasive potential of cells and thereby differentiating between normal and cancerous cells in accordance with the susceptibility of the cellular chromatin to degradation or other modification by particular enzymes or agents. Chromatin within permeabilized normal and non-invasive cells, including chromatin strands removed therefrom, are more susceptible to degradation by endonucleases or proteinases than is the chromatin from invasive cells.
摘要翻译：本发明的实施方案涉及用于评估或估计细胞的侵袭潜力并因此根据细胞染色质对特定酶或试剂的降解或其它修饰的敏感性的正常细胞和癌细胞之间的差异的组合物和方法。在透化的正常和非侵入性细胞（包括从其中去除的染色质丝线）上的染色质比来自浸润细胞的染色质更易受内切核酸酶或蛋白酶降解。

39. 发明申请

WO03048300A2 METHODS OF IDENTIFYING CELLULAR TARGET MOLECULES 审中-公开
标题翻译：鉴定细胞目标分子的方法
公开(公告)号：WO03048300A2
公开(公告)日：2003-06-12
申请号：PCT/US0227831
申请日：2002-08-30
申请人： AMNIS CORP , FINCH ROSALYNDE J
发明人： FINCH ROSALYNDE J
IPC分类号： G01N33/566 , C12Q1/02 , C12Q1/68 , G01N33/53 , G01N33/536 , G01N33/569 , G01N33/68 , C12N
CPC分类号： G01N33/6863 , C12Q1/6841 , G01N33/56966 , C12Q2565/626 , C12Q2527/125
摘要： The present invention provides methods of detecting and/or quantifying specific cellular target molecules in intact cells. The present invention further provides methods of processing an intact cell to facilitate in situ hybridization for use in flow cytometry.
摘要翻译：本发明提供在完整细胞中检测和/或定量特异性细胞靶分子的方法。本发明还提供了处理完整细胞以促进原位杂交以用于流式细胞术的方法。

40. 发明申请

WO0231182A3 METHOD AND APPARATUS FOR READING REPORTER LABELED BEADS 审中-公开
标题翻译：读取报告器标签的方法和装置
公开(公告)号：WO0231182A3
公开(公告)日：2002-09-06
申请号：PCT/US0142638
申请日：2001-10-12
申请人： AMNIS CORP , FROST KEITH , BASIJI DAVID , BAUER RICHARD , FINCH ROSALYNDE , ORTYN WILLIAM , PERRY DAVID
发明人： FROST KEITH , BASIJI DAVID , BAUER RICHARD , FINCH ROSALYNDE , ORTYN WILLIAM , PERRY DAVID
IPC分类号： G01N21/64 , C07B61/00 , C12N15/09 , C12Q1/68 , G01N15/02 , G01N15/10 , G01N15/14 , G01N21/05 , G01N33/48 , G01N33/483 , G01N33/50 , G02B7/28 , G02B21/00 , G02B27/00 , G02B27/14 , G01N33/543 , C07H21/04 , G01J3/28 , G01N21/00 , G01N33/53
CPC分类号： G02B27/148 , B01J2219/00274 , C07B2200/11 , C12Q1/6816 , C12Q1/6869 , C12Q2600/156 , C40B20/04 , C40B60/00 , G01N15/147 , G01N15/1475 , G01N21/6428 , G01N21/6456 , G01N2015/0294 , G01N2015/1018 , G01N2015/1472 , G01N2015/1479 , G01N2021/058 , G01N2021/6421 , G02B7/28 , G02B21/00 , G02B27/0012 , G02B27/1013 , G02B27/126 , G02B27/144 , G02B27/145 , C12Q2565/626 , C12Q2563/149 , C12Q2563/107
摘要： Combinatorially-synthesized deoxyribonucleic acid (DNA) oligonucleotides attached to encoded beads that are hybridized to amplified and labeled genomic DNA or ribonucleic acid (RNA) are analyzed using a flow imaging system. Oligonucleotides and corresponding reporters are bound to the surfaces of a plurality of small beads such that different beads bear different oligo sequences. Each bead bears a unique optical signature comprising a predefined number of unique reporters, where each reporter comprises a predefined combination of different fluorochromes. The composite spectral signature in turn identifies the unique nucleotide sequence of its attached oligo chains. This optical signature is rapidly decoded using an imaging system to discriminate the different reporters attached to each bead in a flow in regard to color and spatial position on the bead (see fig. 15).
摘要翻译：使用流动成像系统分析与编码的珠偶联的与扩增和标记的基因组DNA或核糖核酸（RNA）杂交的组合合成的脱氧核糖核酸（DNA）寡核苷酸。寡核苷酸和相应的报道结合到多个小珠的表面，使得不同的珠具有不同的寡核苷酸序列。每个珠具有独特的光学特征，其包含预定数量的唯一记录器，其中每个报告者包括不同荧光染料的预定组合。复合光谱特征依次识别其连接的寡链的唯一核苷酸序列。使用成像系统快速解码该光学签名，以鉴别与珠上的颜色和空间位置相关的流中附着于每个珠的不同报告者（参见图15）。

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