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31. 发明申请

WO00024931A2 DETECTION OF ANALYTES 审中-公开
标题翻译：检测分析
公开(公告)号：WO00024931A2
公开(公告)日：2000-05-04
申请号：PCT/IL1999/000557
申请日：1999-10-22
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6804 , C12Q1/6862 , C12Q1/6865 , C12Q2545/113 , C12Q2521/337
摘要： The invention concerns a method where catalytic nucleic acid sequence in the presence of an analyte (which may be a nucleic acid sequence or a non-nucleic acid sequence) converts a substrate to a catalytic product. The catalytic product may then be amplified by state of the art amplification methods such as PCR, LCR, 3SR and NASBA.
摘要翻译：本发明涉及一种方法，其中在分析物（其可以是核酸序列或非核酸序列）存在下的催化核酸序列将底物转化为催化产物。然后可以通过现有技术的扩增方法如PCR，LCR，3SR和NASBA扩增催化产物。

32. 发明申请

WO2018027238A1 METHOD FOR DETECTING NUCLEOTIDE POLYMORPHISMS 审中-公开
标题翻译：检测核苷酸多态性的方法
公开(公告)号：WO2018027238A1
公开(公告)日：2018-02-08
申请号：PCT/US2017/045802
申请日：2017-08-07
申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
发明人： MARTINEAU, Rhett, L. , MELDRUM, Deirdre
IPC分类号： C12N9/12 , C12N15/09 , C12P19/34 , C12Q1/02 , C12Q1/68
CPC分类号： C12Q1/6858 , C12N9/12 , C12N15/09 , C12Q1/6883 , C12Q2600/156 , C12Q2525/301 , C12Q2531/119 , C12Q2545/113 , C12Q2563/159
摘要： Methods for detecting single nucleotide polymorphisms in nucleotide sequences using LAMP reactions are provided herein. Generally, two sets of LAMP primers, a wild-type primer that matches expected DNA sequences and an SNP primer that matches the expected SNP DNA are provided. One method includes providing the wild-type primer and the SNP primer in separate wells of a multi-well microfluidic array device, adding the sample nucleotide sequence into the wells seeded with the primers, and initiating LAMP reactions within the wells. The method includes observing the reaction differential between the primers and determining the status of the DNA with regard to that particular SNP. A second method includes providing the primers with tags in a mixture, adding the sample nucleotide sequence to the mixture, and initiating LAMP reactions. The method includes providing a different visual indication when the wild-type primer reacts with the sample nucleotide sequence versus when the SNP primer reacts with the sample nucleotide sequence, and determining the status of the DNA with regard to that particular SNP.
摘要翻译：本文提供了使用LAMP反应检测核苷酸序列中的单核苷酸多态性的方法。通常，提供两组LAMP引物，匹配预期DNA序列的野生型引物和匹配预期SNP DNA的SNP引物。一种方法包括在多孔微流阵列装置的分开的孔中提供野生型引物和SNP引物，将样品核苷酸序列加入用引物接种的孔中，并在孔内启动LAMP反应。该方法包括观察引物之间的反应差异并确定关于该特定SNP的DNA的状态。第二种方法包括为引物提供混合物中的标签，将样品核苷酸序列加入到混合物中，并启动LAMP反应。所述方法包括当野生型引物与样品核苷酸序列反应时与SNP引物与样品核苷酸序列反应并确定关于该特定SNP的DNA状态时提供不同的视觉指示。

33. 发明申请

WO2015051888A1 METHOD FOR THE QUANTITATIVE ANALYSIS OF NUCLEIC ACID FRAGMENTATION AND AMPLIFICABILITY 审中-公开
标题翻译：用于定量分析核酸分解和扩增性的方法
公开(公告)号：WO2015051888A1
公开(公告)日：2015-04-16
申请号：PCT/EP2014/002657
申请日：2014-09-30
申请人： UNIVERSITÄT HEIDELBERG
发明人： NEUMAIER, Michael , AHMAD NEJAD, Parviz
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6851 , C12Q2600/16 , C12Q2537/16 , C12Q2537/165 , C12Q2545/113 , C12Q2565/301
摘要： The present invention relates to a method for the quantitative analysis of complex nucleic acids (NA), i.e. their fragmentation/degradation and amplificability as a marker of biomolecular quality and integrity of a biosample. Said method comprises the steps of subjecting said NA to a multiplex polymerase chain reaction using primers to generate different-size amplicons (referred to as indicator PCR). For simplicity, a duplex PCR using one primer pair for the generation of a longer PCR product and a second primer pair for the generation of a shorter PCR product is being described as the most simple variant of this test. Following the duplex PCR amplification, the ratio between the yield of the longer PCR product and the yield of the shorter PCR product generated during duplex PCR is determined using a read-out that allows relative quantification between the two (e.g. Pyrosequencing). The ratio is proportional to the nucleic acids quality, because the larger fragment tends to be under-represented with increased fragmentation impeding with its amplificability. The invention further relates to the generation and use of reference high-molecular weight DNA samples subjected to degradation under controlled conditions (e.g. by inflicting heat for specified periods of time) to generate a degradation calibration curve. The fragmentation of a query NA sample previously prepared from a liquid or solid biosource can then be quantified by use of the duplex indicator PCR after direct comparison to the calibrator DNA fragmentation curve. The present invention further relates to a comprehensive kit containing all specific components required to apply said method.
摘要翻译：本发明涉及用于定量分析复合核酸（NA）的方法，即其作为生物样品的生物分子质量和完整性的标记物的分裂/降解和可扩增性。所述方法包括以下步骤：使用引物对所述NA进行多重聚合酶链反应以产生不同大小的扩增子（称为指示性PCR）。为了简单起见，使用一个引物对用于产生较长PCR产物的双链PCR和用于产生较短PCR产物的第二引物对被描述为该测试的最简单的变体。在双链PCR扩增之后，使用允许两者之间的相对定量（例如焦磷酸测序）的读数确定较长PCR产物的产量与双相PCR期间产生的较短PCR产物的产量之间的比率。该比例与核酸质量成比例，因为较大的片段倾向于低度代表，随着其可扩增性阻碍增加的片段化。本发明还涉及在受控条件下（例如通过施加热量达指定时间段）进行降解的参考高分子量DNA样品的产生和使用，以产生降解校准曲线。然后可以通过在与校准物DNA断裂曲线直接比较之后使用双链体指示剂PCR来量化从液体或固体生物源预先制备的查询NA样品的片段化。本发明还涉及包含应用所述方法所需的所有特定部件的综合试剂盒。

34. 发明申请

WO2015048535A1 PRENATAL DIAGNOSTIC RESTING STANDARDS 审中-公开
标题翻译：初步诊断标准
公开(公告)号：WO2015048535A1
公开(公告)日：2015-04-02
申请号：PCT/US2014/057843
申请日：2014-09-26
申请人： NATERA, INC.
发明人： BABIARZ, Joshua , ZIMMERMAN, Bernhard
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12N15/10 , C12Q1/6806 , C12Q1/6883 , C12Q2521/301 , C12Q2537/16 , C12Q2545/113 , C12Q2600/16 , C12Q2600/166 , Y10T436/10
摘要： Embodiments of the invention include methods and compositions for producing proficiency testing standards for noninvasive prenatal genetic diagnostics and for the detection and monitoring of cancer. The compositions can comprise a plurality of different nucleosomal DNA fragments derived from either primary cells or cell lines. The amount of the different nucleosomal DNA fragments can be varied so as to simulate naturally occurring cell free DNA samples obtained from the blood of the pregnant woman or naturally occurring cell free DNA samples obtained from the blood of cancer patients.
摘要翻译：本发明的实施方案包括用于生产无创产前遗传诊断能力检测标准和用于检测和监测癌症的方法和组合物。组合物可以包含衍生自原代细胞或细胞系的多种不同的核小体DNA片段。可以改变不同核小体DNA片段的量，以模拟从孕妇的血液中获得的天然存在的无细胞DNA样品或从癌症患者的血液获得的天然存在的无细胞DNA样品。

35. 发明申请

WO2014113843A1 AN ASSAY FOR QUANTITATING THE EXTENT OF METHYLATION OF A TARGET SITE 审中-公开
标题翻译：用于定量目标场地甲基化程度的测定
公开(公告)号：WO2014113843A1
公开(公告)日：2014-07-31
申请号：PCT/AU2014/000044
申请日：2014-01-24
申请人： MURDOCH CHILDRENS RESEARCH INSTITUTE
发明人： GODLER, David , INABA, Yoshimi
IPC分类号： C12Q1/68 , G06F19/22
CPC分类号： C12Q1/6883 , C12Q1/6851 , C12Q2600/154 , G06F19/24 , C12Q2523/125 , C12Q2527/107 , C12Q2545/113 , C12Q2561/113
摘要： The present specification relates to an assay to quantitate extent of methylation in a DNA sample. Kits and clinical diagnostic assays are also enabled herein including assays to determine clinical phenotypes based on extent of methylation of a DNA target site.
摘要翻译：本说明书涉及定量DNA样品中甲基化程度的测定。试剂盒和临床诊断测定法也在本文中实现，包括基于DNA靶位点的甲基化程度确定临床表型的测定法。

36. 发明申请

WO2013020714A2 CELL- OR VIRUS SIMULATING MEANS COMPRISING ENCAPSULATED MARKER MOLECULES 审中-公开
标题翻译：细胞或病毒模拟包含封装标记分子的手段
公开(公告)号：WO2013020714A2
公开(公告)日：2013-02-14
申请号：PCT/EP2012003448
申请日：2012-08-13
申请人： QIAGEN GMBH , QIAGEN HAMBURG GMBH , BELLER KATHARINA , DOEDT THOMAS , HECKEL DIRK , SOELLER RAINER
发明人： BELLER KATHARINA , DOEDT THOMAS , HECKEL DIRK , SOELLER RAINER
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12Q1/6811 , C12Q1/6806 , C12Q1/6848 , C12Q2545/113 , C12Q2547/107 , C12Q2563/149 , C12Q2563/179
摘要： The present invention refers to a method, a composition and a kit for isolating biomolecules from any biological sample material containing cells, virus(es), microorganism(s) or a combination thereof comprising a cell- or virus-simulating means, wherein said cell- or virus- simulating means comprises at least one type of marker molecule(s), incorporated in at least one type of a layer, capsule, bead, sphere or particle, which is not a biological cell or provided on a substrate covered by a coating.
摘要翻译：本发明涉及用于从包含细胞，病毒，微生物或其组合的任何生物样品材料中分离生物分子的方法，组合物和试剂盒，其包含细胞或病毒模拟手段，其中所述细胞 - 或病毒模拟装置包含至少一种类型的标记物分子，所述至少一种类型的标记物分子被结合到至少一种类型的层，囊，珠，球或颗粒中，所述层不是生物细胞或设置在覆盖有涂层。

37. 发明申请

WO2013003849A2 PROCESS AND APPARATUS FOR QUANTIFYING NUCLEIC ACID IN A SAMPLE 审中-公开
标题翻译：量化样品中核酸的方法和设备
公开(公告)号：WO2013003849A2
公开(公告)日：2013-01-03
申请号：PCT/US2012/045271
申请日：2012-07-02
申请人： BIOQUANTA SA , BIOQUANTA, CORP. , ASSISTANCE PUBLIQUE-HOPITAUX DE PARIS , CONTI, Marc , LORIC, Sylvain , MANIVET, Philippe , DELACOTTE, Nicolas , KEUMEUGNI KWEMO, Carlosse , SALIOU BAH, Mamadou
发明人： CONTI, Marc , LORIC, Sylvain , MANIVET, Philippe , DELACOTTE, Nicolas , KEUMEUGNI KWEMO, Carlosse , SALIOU BAH, Mamadou
CPC分类号： C12Q1/6806 , C12Q1/68 , C12Q2537/125 , C12Q2545/113 , C12Q2563/173 , C12Q2522/101 , C12Q2537/161 , C12Q2545/114
摘要： In one aspect, the present invention concerns methods and kits for quantifying a target nucleic acid in a sample. In embodiments, a method comprises sequestrating the target nucleic acid by one or more interactors to form a sequestered conjugate that will be further labeled to form a labeled conjugate. The signal produced by the labeled conjugate is measured and correlated to the amount of the target nucleic acid. In another aspect, the present invention concerns a method for quantifying a target nucleic acid in a sample by a reaction of the nucleic acids in the sample with a nucleic acid interactor or set of interactors to form a conjugate. The conjugate is then sequestered from the rest of the sample with a molecule bound to a support, to form a sequestered conjugate. The sequestered conjugate is labeled to form a labeled conjugate. The signal produced by the labeled conjugate is measured and correlated to the amount of the target nucleic acid. In some embodiments, the target nucleic acid is cell free nucleic acids. In embodiments, the interactor binds to nucleic acids in the sample in a nonsequence specific manner.
摘要翻译：在一个方面，本发明涉及用于定量样品中的靶核酸的方法和试剂盒。在实施方案中，方法包括通过一种或多种相互作用物螯合靶核酸以形成螯合缀合物，所述螯合缀合物将被进一步标记以形成标记缀合物。测量由标记的缀合物产生的信号并与靶核酸的量相关。另一方面，本发明涉及通过样品中的核酸与核酸相互作用物或一组相互作用物反应以形成缀合物来定量样品中的靶核酸的方法。然后将缀合物从与样品的其余部分隔离的分子结合到支持物上，形成螯合的缀合物。将螯合的缀合物标记以形成标记的缀合物。测量由标记的缀合物产生的信号并与靶核酸的量相关。在一些实施方案中，靶核酸是无细胞核酸。在实施方案中，相互作用子以非序列特异性方式结合样品中的核酸。

38. 发明申请

WO2012135125A1 TELOMERE LENGTH MEASUREMENT IN FORMALIN-FIXED, PARAFFIN EMBEDDED (FFPE) SAMPLES BY QUANTITATIVE PCR 审中-公开
标题翻译：通过定量PCR在FORMALIN-FIXED，PARAFFIN嵌入（FFPE）样品中的TELOMERE长度测量
公开(公告)号：WO2012135125A1
公开(公告)日：2012-10-04
申请号：PCT/US2012/030581
申请日：2012-03-26
申请人： GERON CORPORATION , WANG, Hui , GO, Ning, F. , PIROT, Zhu, Zhen
发明人： WANG, Hui , GO, Ning, F. , PIROT, Zhu, Zhen
IPC分类号： G01N33/574
CPC分类号： C12Q1/6876 , C12Q1/6851 , G01N33/5088 , G01N2800/52 , C12Q2525/155 , C12Q2527/107 , C12Q2527/125 , C12Q2527/137 , C12Q2545/113
摘要： Methods of reliably quantifying telomere length in cells or tissues that have been formalin fixed and paraffin embedded (FFPE) samples by quantitative polymerase chain reaction protocol and kits for use with such various methods are provided. The methods of the present invention may be used to predetermine an individual's response to treatment with a telomerase inhibitor, a telomere damaging agent or a telomerase activator.
摘要翻译：提供了通过定量聚合酶链反应方案和用于这些各种方法的试剂盒可靠地定量已经用福尔马林固定和石蜡包埋（FFPE）样品的细胞或组织中端粒长度的方法。本发明的方法可以用于预先确定个体对用端粒酶抑制剂，端粒损伤剂或端粒酶激活剂治疗的反应。

39. 发明申请

WO2012071344A2 NUCLEOTIDE-BASED PROBES AND METHODS FOR THE DETECTION AND QUANTIFICATION OF MACROMOLECULES AND OTHER ANALYTES 审中-公开
标题翻译：基于核苷酸的探针和方法用于检测和定量大分子和其他分析物
公开(公告)号：WO2012071344A2
公开(公告)日：2012-05-31
申请号：PCT/US2011/061701
申请日：2011-11-21
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , VALLEE-BELISLE, Alexis , RICCI, Francesco , WHITE, Ryan , BONHAM, Andrew J. , PLAXCO, Kevin W.
发明人： VALLEE-BELISLE, Alexis , RICCI, Francesco , WHITE, Ryan , BONHAM, Andrew J. , PLAXCO, Kevin W.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6825 , C12Q1/6818 , C12Q2525/301 , C12Q2565/1015 , C12Q2565/1025 , C12Q2545/113 , C12Q2563/131
摘要： Provided are unimolecular oligonucleotide probes for detecting a target in a sample. The probes use target binding-induced structural changes to detect the presence of the target in the sample. Also provided are methods of using the probes to detect a target in a sample.
摘要翻译：提供了用于检测样品中靶标的单分子寡核苷酸探针。探针使用靶结合诱导的结构变化来检测样品中目标物的存在。还提供了使用探针来检测样品中的靶的方法。

40. 发明申请

WO2012009813A1 METHODS OF DETECTING GENETIC POLYMORPHISMS USING MELTING TEMPERATURE OF PROBE-AMPLICON COMPLEXES 审中-公开
标题翻译：使用探针 - AMPLICON复合物的熔融温度检测遗传多态性的方法
公开(公告)号：WO2012009813A1
公开(公告)日：2012-01-26
申请号：PCT/CA2011/050443
申请日：2011-07-20
申请人： SOCPRA SCIENCES SANTE ET HUMAINES, S.E.C. , FROST, Eric , DESLANDES, Sylvie , DEXTRAS PAQUETTE, Patrick
发明人： FROST, Eric , DESLANDES, Sylvie , DEXTRAS PAQUETTE, Patrick
IPC分类号： C12Q1/68 , C12P19/34 , C40B30/04
CPC分类号： C12Q1/689 , C12Q1/6818 , C12Q1/6827 , C12Q1/701 , C12Q2600/156 , C12Q2600/16 , C12Q2545/113 , C12Q2535/131 , C12Q2527/107 , C12Q2565/1015 , C12Q2537/143
摘要： The present application provides methods and related kits for the detection of a nucleotide difference between a target sequence and standard sequences. The methods include the determination of a melting temperature of a complex between an amplicon of the target sequence and a probe which is substantially identical to one of the standard sequences. The probe comprises a detectable label, linked to its 5' end as well as a quencher, linked to its 3' end. This method can be easily adapted for use with multiple probes and in multiplex PCR.
摘要翻译：本申请提供了用于检测靶序列和标准序列之间的核苷酸差异的方法和相关试剂盒。所述方法包括确定靶序列的扩增子与基本上与标准序列之一基本相同的探针之间的复合物的解链温度。该探针包含与其5'端连接的可检测标记以及连接于其3'末端的猝灭剂。该方法可以容易地适用于多个探针和多重PCR。

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