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11. 发明申请

WO0073413A9 APPARATUS AND METHOD FOR CELL DISRUPTION 审中-公开
标题翻译：细胞破坏的装置和方法
公开(公告)号：WO0073413A9
公开(公告)日：2002-01-24
申请号：PCT/US0014740
申请日：2000-05-30
申请人： CEPHEID
发明人： TAYLOR MICHAEL T , BELGRADER PHILLIP , POURAHMADI FRAZAD , MCMILLAN WILLIAM A , CHANG RONALD , SAKAI STANLEY H , CHING JESUS , PETERSEN KURT E , NORTHRUP M ALLEN
IPC分类号： C12M1/33 , B01L3/00 , B01L7/00 , C12M1/26 , C12N1/00 , G01N1/04 , G01N1/10 , G01N33/48 , C12M3/08
CPC分类号： B01L3/502 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0809 , B01L2300/087 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12N1/066 , C12N13/00 , Y10S435/81 , Y10S436/805 , Y10S436/806 , Y10S436/807 , Y10S436/809 , Y10S436/829 , Y10T137/87716 , Y10T137/88046 , Y10T436/255
摘要： An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.
摘要翻译：用于破坏细胞或病毒的装置包括具有用于保持细胞或病毒的室的容器。容器包括限定腔室的至少一个柔性壁。该装置还包括用于冲击柔性壁的外表面的换能器，以在腔室中产生压力波。该装置还包括用于增加腔室中的压力的压力源。室的加压确保换能器与柔性壁之间的有效耦合。该装置还可以包括室中的珠子，用于破裂细胞或病毒。

12. 发明申请

WO0072019A3 LIPOSOME-ENHANCED TEST DEVICE AND METHOD 审中-公开
标题翻译： LIPOSOME增强测试装置和方法
公开(公告)号：WO0072019A3
公开(公告)日：2001-09-13
申请号：PCT/US0013592
申请日：2000-05-18
申请人： CORNELL RES FOUNDATION INC , INNOVATIVE BIOTECHNOLOGIES INT
发明人： DURST RICHARD ALLEN , MONTAGNA RICHARD A , BAUMNER ANTJE J , SIEBERT SUI TI A , RULE GEOFFREY S
IPC分类号： C12Q1/68 , G01N33/543 , G01N33/558 , G01N33/58
CPC分类号： C12Q1/6816 , G01N33/5438 , G01N33/558 , G01N33/586 , G01N2333/37 , G01N2458/30 , Y10S435/97 , Y10S436/806 , C12Q2565/607 , C12Q2565/501
摘要： A test device and method for detecting or quantifying an analyte in a test sample employs an interdigitated electrode array and electroactive marker-encapsulating liposomes for signal generation and detection. The test device includes a contact portion on a first absorbent material, a capture portion either on the first absorbent material, or on a second absorbent material in fluid flow contact with the first absorbent material. The capture portion has a binding material specific for a portion of the analyte bound thereto. The device further includes an electrode array including first and second conductors each having a plurality of fingers, wherein the fingers of the conductors are interdigitated. The electrode array is positioned to induce redox cycling of an electroactive marker released either in or beyond the capture portion, depending upon whether direct (proportional) or indirect (inversely proportional) detection or measurement is desired. In the method of the invention, the test sample is applied to the contact portion, and allowed to migrate along the absorbent material(s) into the capture portion. Either before or after the migration, the test sample is contacted with a conjugate of liposomes and a second binding material for the analyte. To the extent that analyte is present in the sample, the conjugate is bound in the capture portion. By applying a voltage across the conductors, redox cycling of the marker is induced and a current is generated.
摘要翻译：用于检测或定量测试样品中的分析物的测试装置和方法使用交叉指示电极阵列和电活性标记物封装的脂质体用于信号产生和检测。测试装置包括在第一吸收材料上的接触部分，或者在第一吸收材料上的捕获部分或与第一吸收材料流体接触的第二吸收材料上。捕获部分具有对与其结合的分析物的一部分特异性的结合材料。该装置还包括电极阵列，该电极阵列包括每个具有多个指状物的第一和第二导体，其中导体的指状物被互相指向。取决于是否需要直接（成比例）或间接（反比例）的检测或测量，定位电极阵列以引发在俘获部分中或超过俘获部分释放的电活性标记物的氧化还原循环。在本发明的方法中，将测试样品施加到接触部分，并使其沿着吸收材料迁移到捕获部分中。在迁移之前或之后，将测试样品与分析物的脂质体和第二结合材料的缀合物接触。在分析物存在于样品中的程度上，缀合物结合在捕获部分中。通过在导体上施加电压，引发标记的氧化还原循环并产生电流。

13. 发明申请

WO00073777A1 ARRAY CYTOMETRY 审中-公开
标题翻译：阵列细胞计数
公开(公告)号：WO00073777A1
公开(公告)日：2000-12-07
申请号：PCT/US2000/014957
申请日：2000-05-28
IPC分类号： G01N33/48 , B01J19/00 , B01L3/00 , C07B61/00 , C12Q1/68 , G01N1/00 , G01N15/14 , G01N27/416 , G01N27/447 , G01N30/00 , G01N33/483 , G01N33/53 , G01N33/543 , G01N33/566 , G01N37/00 , G02B3/00 , G21K1/00 , H05H3/04 , G01N27/00 , B01J19/08 , B29D22/00 , C12M1/26 , G01N15/06 , G01N21/03 , G01N21/29 , G01N27/02 , G01N27/26 , H01M2/38 , H01M4/02 , H01M4/36 , H01M6/04 , H01M6/30 , H01M6/36
CPC分类号： B01J19/0046 , B01J2219/00585 , B01J2219/00596 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00707 , B01J2219/00713 , B01L3/502707 , B01L3/502761 , B01L3/502784 , B01L2200/0647 , B01L2200/0668 , B01L2300/0636 , B01L2300/0816 , B01L2300/089 , B01L2400/0415 , B01L2400/0454 , C12Q1/6825 , C12Q1/6837 , C40B40/00 , G01N15/14 , G01N30/0005 , G01N33/54313 , G01N33/5438 , G01N2015/149 , G02B3/0012 , G02B3/0056 , H05H3/04 , Y10S436/806 , Y10S436/807 , C12Q2565/501 , C12Q2563/155 , C12Q2565/607
摘要： A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled elektrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components. This invention is also for a method and apparatus to direct the lateral motion and induce the assembly into planar arrays of cells on semiconductor surfaces in response to temporally and spatially varying electric fields and to projected patterns of illumination.
摘要翻译：一种用于在诸如氧化硅的绝缘电极和电解质溶液之间的界面处操作胶体颗粒和生物分子的方法和装置。表面附近颗粒的光控电动组装依赖于三种功能元素的组合：交流电场引起的平面聚集体组装; 电解质/氧化硅/硅界面的图案化以在组装过程上施加空间控制; 并通过外部照明实现对装配过程的实时控制。本发明提供一组基本操作，使得能够对几种类型的颗粒和生物分子的平面阵列的创建和放置以及阵列形状和尺寸的操纵进行交互式控制。本发明使得能够以阵列格式进行诊断测定和生物化学分析的样品制备和处理以及这些操作的功能整合。此外，本发明提供了用于产生具有所需性质的材料表面和用于制造表面安装光学部件的步骤。本发明还涉及一种方法和装置，以响应于时间和空间上变化的电场以及投影的照明模式，引导横向运动并将组件引导到半导体表面上的平面阵列阵列。

14. 发明申请

WO0031539A9 METHOD AND APPARATUS FOR PERFORMING A LATERAL FLOW ASSAY 审中-公开
标题翻译：用于执行侧流测定的方法和装置
公开(公告)号：WO0031539A9
公开(公告)日：2000-12-07
申请号：PCT/US9927595
申请日：1999-11-18
申请人： PRAXSYS BIOSYSTEMS INC , POLITO ALAN J , THAYER RICHARD M , DINELLO ROBERT K , SIERRA GEORGE H , NIXON DENNIS , PHILLIPS ALAN , NEUBARTH STUART
发明人： POLITO ALAN J , THAYER RICHARD M , DINELLO ROBERT K , SIERRA GEORGE H , NIXON DENNIS , PHILLIPS ALAN , NEUBARTH STUART
IPC分类号： G01N33/543 , G01N33/558
CPC分类号： G01N27/227 , B01L3/5023 , G01N33/4875 , G01N33/54386 , G01N33/54393 , G01N33/558 , Y10S436/806 , Y10S436/807 , Y10T436/114998
摘要： An embodiment of the present invention provides a method for performing a lateral flow assay. The method includes depositing a sample on a test strip at an application region, detecting a first detection signal arising from the test strip in the first detection zone, and generating a baseline for the first measurement zone by interpolating between values of the detection signal outside of the first measurement zone and inside of the first detection zone. The method may include locating a beginning boundary and an ending boundary for the first measurement zone on the test strip. Additional detection zones having measurement zones may also be incorporated with the embodiment.
摘要翻译：本发明的一个实施方案提供了一种用于进行侧流测定的方法。该方法包括将样品沉积在应用区域的测试条上，检测在第一检测区域中由测试条产生的第一检测信号，以及通过在第一测量区域之外的检测信号的值之间进行内插来生成第一测量区域的基线第一测量区域和第一检测区域内部。该方法可以包括在测试条上定位第一测量区的开始边界和结束边界。具有测量区域的附加检测区域也可以结合实施例。

15. 发明申请

WO1997042503A1 METHOD AND INSTALLATIONS FOR SEPARATING MAGNETIC PARTICLES IN A FLUID FOR BIOLOGICAL ANALYSIS, AND APPLICATION OF SAID METHOD 审中-公开
标题翻译：用于分离用于生物分析的流体中的磁性颗粒的方法和装置以及使用方法
公开(公告)号：WO1997042503A1
公开(公告)日：1997-11-13
申请号：PCT/FR1997000794
申请日：1997-05-05
申请人： BIOCOM S.A. , BISCONTE DE SAINT JULIEN, Jean-Claude
发明人： BIOCOM S.A.
IPC分类号： G01N33/543
CPC分类号： G01N1/405 , B03C1/035 , G01N33/54326 , G01N35/0098 , Y10S435/971 , Y10S436/806 , Y10S436/807 , Y10S436/81 , Y10S436/824
摘要： The invention discloses a magnetic immunoseparation method of cells, particularly of bacteria, foetal cells, bone marrow stem cells and systemic cancerous cells, consisting in fixing target cells on paramagnetic balls and causing a magnetic field to act on a sample containing the fixed cells, the free cells and the supernumerary paramagnetic balls, to isolate the paramagnetic balls. The sample is circulated in a tube (2), the cross-section of which is much less than the length on which the magnetic field is applied.
摘要翻译：本发明公开了一种细胞，特别是细菌，胎儿细胞，骨髓干细胞和全身性癌细胞的磁免疫分离方法，其包括将靶细胞固定在顺磁球上，并使磁场作用于含有固定细胞的样品，游离细胞和超顺磁球，以分离顺磁球。样品在管（2）中循环，其横截面远小于施加磁场的长度。

16. 发明申请

WO1997033176A1 GRAPHITIC NANOTUBES IN LUMINESCENCE ASSAYS 审中-公开
标题翻译：光泽纳米粒子在光度测定中的应用
公开(公告)号：WO1997033176A1
公开(公告)日：1997-09-12
申请号：PCT/US1997003653
申请日：1997-03-05
申请人： IGEN, INC.
发明人： IGEN, INC. , MASSEY, Richard, J. , MARTIN, Mark, T. , DONG, Liwen , LU, Ming , FISCHER, Alan , JAMEISON, Fabian , LIANG, Pam , HOCH, Robert , LELAND, Jonathon, K.
IPC分类号： G01N33/573
CPC分类号： C01B31/0213 , B82Y30/00 , B82Y40/00 , C01B32/152 , C01B32/174 , C01B2202/34 , C01B2202/36 , C07K17/06 , D01F9/12 , D01F11/14 , G01N21/69 , G01N21/76 , G01N33/54373 , G01N33/5438 , G01N33/582 , G01N2030/8863 , G11C13/025 , H01M4/60 , Y10S435/968 , Y10S436/805 , Y10S436/806 , Y10S977/737 , Y10S977/742
摘要： Graphitic nanotubes, which include tubular fullerenes (commonly called "buckytubes") and fibrils, which are functionalized by chemical substitution, are used as solid supports in electrogenerated chemiluminescence assays. The graphitic nanotubes are chemically modified with functional group biomolecules prior to use in an assay. Association of electrochemiluminescent ruthenium complexes with the functional group biomolecule-modified nanotubes permits detection of molecules including nucleic acids, antigens, enzymes, and enzyme substrates by multiple formats.
摘要翻译：石墨纳米管，其包括通过化学取代官能化的管状富勒烯（通常称为“buckytubes”）和原纤维，被用作电致化学发光测定中的固体支持物。石墨纳米管在用于测定之前用官能团生物分子进行化学修饰。电化学发光钌络合物与官能团生物分子修饰的纳米管的关联允许通过多种形式检测包括核酸，抗原，酶和酶底物的分子。

17. 发明申请

WO1996028538A1 MULTI-ARRAY, MULTI-SPECIFIC ELECTROCHEMILUMINESCENCE TESTING 审中-公开
标题翻译：多阵列，多特定电子显微镜测试
公开(公告)号：WO1996028538A1
公开(公告)日：1996-09-19
申请号：PCT/US1996003190
申请日：1996-03-06
申请人： MESO SCALE TECHNOLOGIES, LLC
发明人： MESO SCALE TECHNOLOGIES, LLC , WOHLSTADTER, Jacob , WILBUR, James , SIGAL, George , MARTIN, Mark , GUO, Liang-Hong , FISCHER, Alan , LELAND, Jon
IPC分类号： C12M01/00
CPC分类号： G01N21/66 , B82Y30/00 , G01N21/76 , G01N33/5438 , G01N33/582 , Y10S436/806 , Y10S977/957
摘要： Materials and methods are provided for producing patterned multi-array, multi-specific surfaces which are electronically excited for use in electrochemiluminescence based tests. Materials and methods are provided for the chemical and/or physical control of conducting domains and reagent deposition for use in flat panel displays and multiply specific testing procedures.
摘要翻译：提供材料和方法用于产生电子激发用于基于电化学发光的测试的图案化多阵列多特异性表面。提供材料和方法用于化学和/或物理控制导电域和试剂沉积，用于平板显示器和多种特定测试程序。

18. 发明申请

WO1996026782A1 IMPROVED MAGNETIC SEPARATION APPARATUS AND METHOD 审中-公开
标题翻译：改进的磁选分离装置和方法
公开(公告)号：WO1996026782A1
公开(公告)日：1996-09-06
申请号：PCT/US1996000953
申请日：1996-01-29
申请人： MILTENYI BIOTECH, INC.
发明人： MILTENYI BIOTECH, INC. , MILTENYI, Stephan
IPC分类号： B01D35/06
CPC分类号： B03C1/002 , A61M1/36 , A61M1/3618 , A61M1/362 , B01D35/06 , B03C1/01 , B03C1/025 , B03C1/027 , B03C1/0332 , B03C1/0335 , B03C1/034 , B03C2201/18 , B03C2201/26 , G01N1/34 , G01N33/54326 , Y10S436/806
摘要： A magnetic separation column (2) and method for magnetic separation are disclosed herein. The separation column (2) includes a fluid permeable matrix (6) which provides uniform channels that reduce the entrapment of air or non-target substances, and decrease the loss of target substances due to mechanical disruption. Biological target cells are labeled in conjunction with a suitable specific binding member and isolated using the method of the present invention.
摘要翻译：本文公开了磁分离柱（2）和磁分离方法。分离柱（2）包括可提供均匀通道的流体可渗透基质（6），其减少空气或非目标物质的滞留，并减少机械破坏导致的目标物质的损失。结合适当的特异性结合成员标记生物靶细胞，并使用本发明的方法进行分离。

19. 发明申请

WO1996012960A1 SEPARATION OF MAGNETIC MICROPARTICLES INVOLVING A PRE-CONCENTRATION STEP 审中-公开
标题翻译：涉及预浓缩步骤的磁性微球的分离
公开(公告)号：WO1996012960A1
公开(公告)日：1996-05-02
申请号：PCT/FI1995000579
申请日：1995-10-20
申请人： LABSYSTEMS OY , TUUNANEN, Jukka
发明人： LABSYSTEMS OY
IPC分类号： G01N33/543
CPC分类号： B03C1/035 , B03C1/28 , G01N33/54326 , G01N35/0098 , Y10S436/806 , Y10T436/25375
摘要： The invention relates to a method for separating magnetic particles from a composition in a vessel. In the method, particles are first concentrated on the wall of the vessel and collected from this area by using a separation means (1) to which the magnetic particles adhere. In this way, particles can be collected efficiently. The invention can be used in different applications especially in the fields of biotechnology, biochemistry, and biomedicine.
摘要翻译：本发明涉及从容器中的组合物中分离出磁性颗粒的方法。在该方法中，首先将颗粒集中在容器的壁上并通过使用磁性颗粒粘附到其上的分离装置（1）从该区域收集。以这种方式，可以有效地收集颗粒。本发明可用于不同的应用，特别是在生物技术，生物化学和生物医学领域。

20. 发明申请

WO1994024561A1 IMMOBILIZED CARBOHYDRATE BIOSENSOR 审中-公开
标题翻译：固化碳水化合物生物传感器
公开(公告)号：WO1994024561A1
公开(公告)日：1994-10-27
申请号：PCT/SE1994000343
申请日：1994-04-18
申请人： NILSSON, Kurt , MANDENIUS, Carl-Fredrik
IPC分类号： G01N33/543
CPC分类号： G01N33/54373 , Y10S436/805 , Y10S436/806 , Y10S436/827
摘要： The present application refers to a biosensor in which an immobilized carbohydrate or a derivative thereof is used to generate a detectable signal when a protein, a virus or a cell is bound to the carbohydrate surface. The sensor is an optical sensor, a piezoelectric sensor, an electrochemical electrode or a thermistor. A method of binding carbohydrates to a gold surface is also described.
摘要翻译：本申请是指当蛋白质，病毒或细胞结合到碳水化合物表面时，使用固定的碳水化合物或其衍生物来产生可检测信号的生物传感器。传感器是光学传感器，压电传感器，电化学电极或热敏电阻。还描述了将碳水化合物结合到金表面的方法。

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