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11. 发明申请

WO2009128557A1 ELECTROCHEMICAL IMMUNOASSAY 审中-公开
标题翻译：电化学免疫
公开(公告)号：WO2009128557A1
公开(公告)日：2009-10-22
申请号：PCT/JP2009/057942
申请日：2009-04-15
申请人： CANON KABUSHIKI KAISHA , KOBAYASHI, Masaaki
发明人： KOBAYASHI, Masaaki
IPC分类号： G01N27/48 , G01N33/543 , G01N33/58
CPC分类号： G01N33/587 , G01N33/54326 , G01N33/5438
摘要： Provided is an immunoassay for detecting a test substance with high sensitivity. The immunoassay is a method of detecting or measuring a test substance contained in a sample liquid, and includes: reacting, with the test substance, a first specifically binding substance which is immobilized on a support and is capable of binding specifically to the test substance and a second specifically binding substance which is labeled with a labeling substance and is capable of binding specifically to the test substance; separating, from the support, the second specifically binding substance which is unreactive with the test substance; eluting the labeling substance retained on the support; depositing the eluted labeling substance on a test element; and measuring electrochemically a catalyst reaction amount of the labeling substance deposited on the test element.
摘要翻译：提供了用于检测具有高灵敏度的测试物质的免疫测定。免疫测定法是检测或测定样品液体中含有的检测物质的方法，其特征在于，包括：将被测物质与固定在载体上并能够特异性结合于试验物质的第一特异性结合物质反应，第二特异性结合物质，其被标记物质标记并且能够特异性结合被检物质; 从载体中分离与测试物质不反应的第二特异性结合物质; 洗脱保留在支持体上的标记物质; 将洗脱的标记物质沉积在测试元件上; 并电化学测定沉积在测试元件上的标记物质的催化剂反应量。

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