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11. 发明申请

WO2008006734A3 UV-SENSITIVE SAFETY STEMS FOR BIOTECHNOLOGICAL PRODUCTION 审中-公开
标题翻译：紫外线敏感的安全梗生物技术生产
公开(公告)号：WO2008006734A3
公开(公告)日：2008-04-03
申请号：PCT/EP2007056684
申请日：2007-07-03
申请人： HENKEL KGAA , WIELAND SUSANNE , MEINHARDT FRIEDHELM , WALDECK JENS , MEYER-RAMMES HEIKE , NAHRSTEDT HANNES
发明人： WIELAND SUSANNE , MEINHARDT FRIEDHELM , WALDECK JENS , MEYER-RAMMES HEIKE , NAHRSTEDT HANNES
IPC分类号： C07K14/32
CPC分类号： C07K14/195 , C12N1/36 , C12N9/00 , C12P21/00
摘要： The aim of the invention is to increase UV sensitivity of Gram-positive bacteria that do not belong to the species Bacillus megaterium. Said aim is achieved by functionally deactivating: a) a part of the uvrBA operon that does not comprise the genes uvrA and uvrB, or b) the genes uvrA and uvrB and/or the gene uvrC of said bacteria or the respective homologues thereof from the respective bacterium. The stems obtained in said manner are particularly UV-sensitive and are thus suitable as safety stems for biotechnological production. Said deactivation can be combined with other deactivation options, especially deactivation of recA and/or deactivation of sporulation genes. Also disclosed are corresponding possible uses for parts of the bacterial uvrBA operon and/or the uvrC gene and for oligonucleotides suitable for carrying out the deactivation process, as well as corresponding bacterial stems and fermentation processes carried out therewith.
摘要翻译：本申请是为了增加其不属于巨大芽胞杆菌物种革兰氏阳性细菌的紫外线灵敏度，所述方法包括：a）将uvrBA操纵子，它不包括基因uvrA和uvrB，或b）的基因uvrA和uvrB的一部分和/或所述基因uvrC这些细菌或从相关的细菌功能性失活它们各自的同源物。由此获得的菌株是特别UV敏感，并因此适合作为生物技术生产安全的菌株。这种失活可以与其他Inaktivierungsmöglichkeiten组合，特别是recA基因的失活和/或失活孢子形成的基因。此外，对应于用途的细菌uvrBA操纵子和/或基因uvrC的部件和用于进行失活示适当的寡核苷酸，和相应的细菌菌株和由此进行的发酵方法。

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