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11. 发明申请

WO2009067279A1 PRODUCTION OF PERACIDS USING AN ENZYME HAVING PERHYDROLYSIS ACTIVITY 审中-公开
标题翻译：使用具有过敏活性的酵素生产过氧化物
公开(公告)号：WO2009067279A1
公开(公告)日：2009-05-28
申请号：PCT/US2008/067712
申请日：2008-06-20
申请人： E.I. DU PONT DE NEMOURS AND COMPANY , DICOSIMO, Robert , GAVAGAN, John, E. , PAYNE, Mark , COOLING, Frederick, B. III
发明人： DICOSIMO, Robert , GAVAGAN, John, E. , PAYNE, Mark , COOLING, Frederick, B. III
IPC分类号： C12P7/40 , C12P7/62 , C12N9/18
CPC分类号： C12P7/40 , A61L2/186 , C12P7/62
摘要： A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided.
摘要翻译：提供了从羧酸酯制备过氧羧酸的方法。更具体地说，羧酸酯与无机过氧化物如过氧化氢在具有过水解活性的酶催化剂存在下反应。基于保守的结构特征，本发明的过水解酶催化剂被分类为碳水化合物酯酶家族7（CE-7）的成员。此外，提供包含通过本文所述方法生产的过酸的消毒剂制剂。

12. 发明申请

WO2002072856A2 IMPROVED PROCESS FOR CONVERTING NITRILES TO CARBOXYLIC ACIDS USING NITRILASE 审中-公开
标题翻译：使用硝酸盐将硝酸盐转化为羧酸的改进方法
公开(公告)号：WO2002072856A2
公开(公告)日：2002-09-19
申请号：PCT/US2002/001811
申请日：2002-01-22
申请人： E.I. DUPONT DE NEMOURS AND COMPANY
发明人： DICOSIMO, Robert , FALL0N, Robert, D. , GAVAGAN, John, E.
IPC分类号： C12P7/00
CPC分类号： C12N1/04 , C12N9/78 , C12N9/96 , C12N11/04 , C12N11/08 , C12N11/10 , C12P7/40 , C12P13/002 , Y02P20/588
摘要： This invention relates to an improved process for preparing a carboxylic acid from the corresponding nitrile using a nitrilase catalyst. More particularly, the instant invention converts 2-methylglutaronitrile to 4-cyanopentanoic acid in aqueous solution using an enzyme catalyst having an nitrilase activity, immobilized in alginate, and crosslinked with glutaraldehyde and polyethylenimine.
摘要翻译：本发明涉及使用腈水解酶催化剂从相应的腈制备羧酸的改进方法。更具体地，本发明使用具有腈水解酶活性的酶催化剂将2-甲基戊二酸转化成水溶液中的4-氰基戊酸，固定在藻酸盐中，并与戊二醛和聚乙烯亚胺交联。

13. 发明申请

WO2002068658A1 METHOD FOR PRODUCING ALPHA-HYDROXY ACID, GLYCOLIC ACID 2-HYDROXYISOBUTYRIC ACID FROM A CORRESPONDING ALPHA-HYDROXY NITRILE USING NITRILASE 审中-公开
标题翻译：使用硝酸盐从相应的阿尔法羟基硝酸生产α-羟基酸，甘油酸2-羟基异丁酸的方法
公开(公告)号：WO2002068658A1
公开(公告)日：2002-09-06
申请号：PCT/US2002/008156
申请日：2002-02-20
申请人： E.I. DUPONT DE NEMOURS AND COMPANY
发明人： CHAUHAN, Sarita , DICOSIMO, Robert , FALL0N, Robert, D. , GAVAGAN, John, E. , PAYNE, Mark, S.
IPC分类号： C12N15/55
CPC分类号： C12P7/42 , C12N9/78
摘要： The present invention relates to a method for producing α-hydroxy acids from the corresponding α-hydroxy nitrile using a catalyst having nitrilase activity. More specifically, the invention pertains to use of Acidovorax facilis 72W (ATCC 55746) nitrilase to hydrolyze glycolonitrile to glycolic acid, or acetone cyanohydrin to 2-hydroxyisobutyric acid. Glycolonitrile is reacted in an aqueous mixture with a catalyst having Acidovorax facilis 72W nitrilase activity to give glycolic acid selectively, and at high concentration and high yield.-hydroxy nitrile using a catalyst having nitrilase activity. More specifically, the invention pertains to use of Acidovorax facilis 72W (ATCC 55746) nitrilase to hydrolyze glycolonitrile to glycolic acid, or acetone cyanohydrin to 2-hydroxyisobutyric acid. Glycolonitrile is reacted in an aqueous mixture with a catalyst having Acidovorax facilis 72W nitrilase activity to give glycolic acid selectively, and at high concentration and high yield.
摘要翻译：本发明涉及使用具有腈水解酶活性的催化剂从相应的α-羟基腈生产α-羟基酸的方法。更具体地，本发明涉及Acidovorax facilis 72W（ATCC 55746）腈水解酶将乙醇腈水解成乙醇酸或丙酮氰醇至2-羟基异丁酸的用途。乙醇腈在水性混合物中与具有Acidovorax facilis 72W腈水解酶活性的催化剂反应，选择性地得到乙醇酸，并且使用具有腈水解酶活性的催化剂以高浓度和高收率产生羟基腈。更具体地，本发明涉及Acidovorax facilis 72W（ATCC 55746）腈水解酶将乙醇腈水解成乙醇酸或丙酮氰醇至2-羟基异丁酸的用途。乙醇腈在含水混合物中与具有酸性酵母72W腈水解酶活性的催化剂反应，选择性地得到乙醇酸，并以高浓度和高产率。

14. 发明申请

WO2002072856A3 IMPROVED PROCESS FOR CONVERTING NITRILES TO CARBOXYLIC ACIDS USING NITRILASE 审中-公开
公开(公告)号：WO2002072856A3
公开(公告)日：2002-09-19
申请号：PCT/US2002/001811
申请日：2002-01-22
申请人： E.I. DUPONT DE NEMOURS AND COMPANY
发明人： DICOSIMO, Robert , FALL0N, Robert, D. , GAVAGAN, John, E.
IPC分类号： C12N11/10
摘要： This invention relates to an improved process for preparing a carboxylic acid from the corresponding nitrile using a nitrilase catalyst. More particularly, the instant invention converts 2-methylglutaronitrile to 4-cyanopentanoic acid in aqueous solution using an enzyme catalyst having an nitrilase activity, immobilized in alginate, and crosslinked with glutaraldehyde and polyethylenimine.

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