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91. 发明申请

WO9318166A3 PRODUCTION OF (ASPERGILLUS NIGER CATALASE-R) 审中-公开
标题翻译：（ASPERGILLUS NIGER CATALASE-R）
公开(公告)号：WO9318166A3
公开(公告)日：1993-10-28
申请号：PCT/US9302020
申请日：1993-03-04
申请人： GENENCOR INT
发明人： BERKA RANDY M , FOWLER TIMOTHY , REY MICHAEL W
IPC分类号： C12N15/09 , C12N1/15 , C12N9/08 , C12N15/00 , C12N15/53 , C12N15/80 , C12R1/68
CPC分类号： C12N9/0065 , C12N15/80
摘要： The invention discloses the application of genetic engineering techniques to create novel strains of A. niger which produce high levels of catalase catR gene product, catalase-R) while generating minimal sodium gluconate waste material. Use of an Aspergillus glucoamylase promoter gene.

92. 发明申请

WO1993020198A1 INTERSEXUAL HETEROZYGOUS PHYCOMYCES 审中-公开
标题翻译：间性杂合植物系
公开(公告)号：WO1993020198A1
公开(公告)日：1993-10-14
申请号：PCT/EP1993000850
申请日：1993-04-05
申请人： GIST-BROCADES N.V. , CERDA-OLMEDO, Enrique , MEHTA, Bina, Jamnadas , VAN DIJCK, Petrus, Wilhelmus, Maria , KOEKMAN, Bertus, Pieter
发明人： GIST-BROCADES N.V.
IPC分类号： C12N15/04
CPC分类号： C12N15/04 , C12N15/80 , C12N15/89 , C12P23/00
摘要： The present invention discloses intersexual heterozygous Phycomyces strains. These strains show an improved beta -carotene productivity and an increased stability when compared with both the wild type and heterokaryotic Phycomyces strains. The present invention also disclosed a method for obtaining Phycomyces transformants without using selection markers. Furthermore, a process is provided for producing beta -carotene comprising the use of intersexual heterozygous Phycomyces strains in a submerged culture.
摘要翻译：本发明公开了异种间杂种Phycomyces菌株。与野生型和异源真菌种菌株相比，这些菌株显示出改善的β-胡萝卜素生产力和增加的稳定性。本发明还公开了一种在不使用选择标记的情况下获得植物分枝杆菌转化体的方法。此外，提供了一种生产β-胡萝卜素的方法，其包括在浸没培养物中使用间性杂合的Phycomyces菌株。

93. 发明申请

WO1993019195A1 A PROCESS FOR PRODUCING HEME PROTEINS 审中-公开
标题翻译：生产HEME蛋白的方法
公开(公告)号：WO1993019195A1
公开(公告)日：1993-09-30
申请号：PCT/DK1993000094
申请日：1993-03-16
申请人： NOVO NORDISK A/S , JENSEN, Ejner, Bech
发明人： NOVO NORDISK A/S
IPC分类号： C12P21/02
CPC分类号： C12N9/0065 , C12N15/80
摘要： Process for producing an extracellular heme protein in increased yields, the process comprising culturing an apoprotein producing microorganism in a fermentation medium containing heme or a heme-containing material under conditions permitting the production of active, recombined heme protein, and recovering the resulting heme protein from the culture medium.
摘要翻译：用于以增加的产量生产细胞外血红素蛋白的方法，所述方法包括在允许产生活性的重组血红素蛋白的条件下，在含有血红素或含血红素的物质的发酵培养基中培养产生脱辅基蛋白的微生物，并将所得血红素蛋白从培养基。

94. 发明申请

WO1993004180A1 PROCESS FOR PURPOSEFULLY MODIFYING THE GENETIC CHARACTERISTICS OF ASHBYA GOSSYPII 审中-公开
标题翻译：用于修改ASHBYA GOSSYPII遗传特征的方法
公开(公告)号：WO1993004180A1
公开(公告)日：1993-03-04
申请号：PCT/EP1992001878
申请日：1992-08-18
申请人： BASF AKTIENGESELLSCHAFT , STEINER, Sabine , WENDLAND, Juergen , WRIGHT, Martin, C. , KURTH, Roland , PHILIPPSEN, Peter
发明人： BASF AKTIENGESELLSCHAFT
IPC分类号： C12N15/90
CPC分类号： C12N15/80 , C12N15/902
摘要： A process is disclosed for purposefully modifying the genetic characteristics of Ashbya gossypii by homologous recombination.
摘要翻译：公开了通过同源重组有目的地改变棉蚜亚种的遗传特征的方法。

95. 发明申请

WO1992016634A1 A PROCESS FOR PRODUCING HEME PROTEINS 审中-公开
标题翻译：生产HEME蛋白的方法
公开(公告)号：WO1992016634A1
公开(公告)日：1992-10-01
申请号：PCT/DK1992000088
申请日：1992-03-20
申请人： NOVO NORDISK A/S , ANDERSEN, Henrik, Dalboge , JENSEN, Ejner, Bech , WELINDER, Karen, Gjesing
发明人： NOVO NORDISK A/S
IPC分类号： C12N15/80
CPC分类号： C12N9/0065 , C07K2319/02 , C12N15/80
摘要： Heterologous heme proteins may be produced extracellularly in filamentous fungi by transforming a filamentous fungus with a vector comprising a DNA sequence encoding a heterologous heme protein and a DNA sequence encoding a preregion permitting secretion of the expressed heme protein, and culturing the transformed filamentous fungus in a suitable culture medium to produce the heme protein.

96. 发明申请

WO1992007079A1 TRANSFORMATION SELECTION MARKER SYSTEM ADAPTED FOR PENICILLIUM 审中-公开
标题翻译：转换选择标记系统适用于PENICILLIUM
公开(公告)号：WO1992007079A1
公开(公告)日：1992-04-30
申请号：PCT/NL1991000203
申请日：1991-10-15
申请人： GIST-BROCADES N.V. , VAN HARTINGSVELDT, Willem , VAN GORCOM, Robert, Franciscus, Maria , GOUKA, Robertus, Johannes , BOVENBERG, Roelof, Ary, Lans
发明人： GIST-BROCADES N.V.
IPC分类号： C12N15/80
CPC分类号： C12N9/93 , C12N15/80
摘要： A transformant selection system has been developed, particularly for a β-lactam producing strain, more particularly for P. chrysogenum, by the complementation of a mutation of said β-lactam producing strain by a homologous selection marker without interfering with β-lactam biosynthesis. Particularly, in applying said transformant selection system a positive selection agent, for instance fluoroacetate is used for the isolation of fac mutants of said strain, particularly of said β-lactam producing strain. Furthermore, a gene entitled facA has been isolated from P. chrysogenum.

97. 发明申请

WO1992000379A1 NEW PROMOTER REGION 审中-公开
标题翻译：新促销区
公开(公告)号：WO1992000379A1
公开(公告)日：1992-01-09
申请号：PCT/EP1991001116
申请日：1991-06-15
申请人： BASF AKTIENGESELLSCHAFT , KURTH, Roland , PHILIPPSEN, Peter , STEINER, Sabine , WRIGHT, Martin, C.
发明人： BASF AKTIENGESELLSCHAFT
IPC分类号： C12N15/80
CPC分类号： C12N15/80 , C07K14/37 , C12P25/00
摘要： Described is the promoter region of the A. gossypii gene which codes the translation elongation factor EF-1α. The promoter can be used in the synthesis of proteins.

98. 发明申请

WO1991006644A1 IMPROVED PLASMID VECTORS FOR CELLULAR SLIME MOULDS OF THE GENUS DICTYOSTELIUM 审中-公开
标题翻译：革兰氏阴性细菌的改进的PLASMID VECTORS
公开(公告)号：WO1991006644A1
公开(公告)日：1991-05-16
申请号：PCT/AU1990000530
申请日：1990-11-02
申请人： MACQUARIE UNIVERSITY , SLADE, Martin, Basil , CHANG, Andy, Chin, Meng , WILLIAMS, Keith, Leslie
发明人： MACQUARIE UNIVERSITY
IPC分类号： C12N15/11
CPC分类号： C07K14/37 , C07K2319/02 , C12N15/80
摘要： The present invention relates generally to the fields of molecular biology and the production of recombinant protein using cellular slime moulds of the genus Dictyostelium. Most particularly, the present invention relates to novel strains of the genus Dictyostelium, recombinant plasmids for use with strains of the genus Dictyostelium, and polypeptides which facilitate the extrachromosomal replication of such plasmids in strains of the genus Dictyostelium. In particular, the present invention provides a polypeptide which facilitates the extrachromosomal replication of a recombinant plasmid in Dictyostelium spp in which the recombinant plasmid includes an origin of replication derived from a Ddp2-like plasmid but which lacks functional genes for extrachromosomal replication in wild type Dictyostelium spp. The extrachromosomal replicating plasmid constructed in accordance with the present invention are suitable for carrying a wide variety of genes and promoter sequences for control production of recombinant proteins by the biotechnology industry.
摘要翻译：本发明一般涉及分子生物学领域和使用盘基网柄菌属的细胞粘液霉菌生产重组蛋白质。最具体地说，本发明涉及盘基网柄菌属的新菌株，用于盘基网柄菌属的菌株的重组质粒，以及促进这种质粒在染色假丝酵母属菌株中的染色体外复制的多肽。特别地，本发明提供了促进重要质粒在盘基网柄菌属spp中的染色体外复制的多肽，其中重组质粒包含衍生自Ddp2样质粒的复制起点，但是在野生型盘基网柄菌属中缺少染色体外复制的功能基因属。根据本发明构建的染色体外复制质粒适用于携带多种基因和启动子序列，用于通过生物技术工业控制重组蛋白的产生。

99. 发明申请

WO1990000192A1 ASPARTIC PROTEINASE DEFICIENT FILAMENTOUS FUNGI 审中-公开
标题翻译：真菌蛋白酶缺乏真菌真菌
公开(公告)号：WO1990000192A1
公开(公告)日：1990-01-11
申请号：PCT/US1989002891
申请日：1989-07-01
申请人： GENENCOR, INC.
发明人： GENENCOR, INC. , BERKA, Randy , HAYENGA, Kirk , LAWLIS, Virgil, Brian , WARD, Michael
IPC分类号： C12N01/15
CPC分类号： C12N9/6481 , A23C19/04 , C12N9/62 , C12N15/80 , C12R1/665
摘要： This invention relates to novel mutant filamentous fungi which are deficient in the gene for the corresponding aspartic proteinase. These organisms are useful production hosts in the production of heterologous polypetides such as chymosin.
摘要翻译：本发明涉及缺乏相应天冬氨酸蛋白酶基因的新型突变丝状真菌。这些生物体是生产异源多肽如凝乳酶的有用生产宿主。

100. 发明申请

WO1986000089A1 EUKARYGOTIC HYBRID VECTORS AND PREPARATION OF POLYPEPTIDES 审中-公开
标题翻译：非线性混合矢量和多肽的制备
公开(公告)号：WO1986000089A1
公开(公告)日：1986-01-03
申请号：PCT/EP1985000278
申请日：1985-06-11
申请人： CIBA-GEIGY AG , HINNEN, Albert , KÜNZLER, Peter
发明人： CIBA-GEIGY AG
IPC分类号： C12N15/00
CPC分类号： C12N9/6459 , C12N15/63 , C12N15/68 , C12N15/80 , C12N15/81 , C12N15/815 , C12N15/82 , C12Y304/21069
摘要： Extrachromosomal ribosomal DNA (rDNA) can be used as a vector for introducing a foreign gene into eukaryotic cells. Eukaryotic hybrid vectors comprising an extrachromosomal rDNA or functional fragments thereof and a foreign structural gene coding for a polypeptide, and methods for their preparation, characterized in that a foreign structural gene coding for a polypeptide and optionally additional DNA sequences are introduced in vitro or in vivo into an extrachromosomal rDNA or functional fragments thereof. A eukaryotic cell transformed with a hybrid vector and methods for the preparation thereof, characterize in that a transformable eukaryotic cell is treated with the eukaryotic hybrid vector under transforming conditions.
摘要翻译：染色体外核糖体DNA（rDNA）可用作将外源基因导入真核细胞的载体。包含染色体外rDNA或其功能片段的真核杂交载体和编码多肽的外源结构基因及其制备方法，其特征在于，在体外或体内引入编码多肽的外源结构基因和任选的其它DNA序列进入染色体外的rDNA或其功能片段。用杂交载体转化的真核细胞及其制备方法，其特征在于在转化条件下用真核杂交载体处理可转化的真核细胞。

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