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1. 发明申请

WO2016140925A1 REGULATORY ELEMENTS FROM LABYRINTHULOMYCETES MICROORGANISMS 审中-公开
标题翻译：乳酸菌微生物的调节元素
公开(公告)号：WO2016140925A1
公开(公告)日：2016-09-09
申请号：PCT/US2016/020114
申请日：2016-02-29
申请人： SYNTHETIC GENOMICS, INC.
发明人： CAIAZZA, Nicky, C. , WIN, Maung, Nyan , URANO, Jun
IPC分类号： C12N15/63 , C12N15/80 , C12N15/113 , C12Q1/68
CPC分类号： C12N15/79 , C12N15/63 , C12N15/80
摘要： The present disclosure generally relates to novel polynucleotide molecules for use in regulating gene expression in recombinant cells, such as labyrinthulomycetes cells. The disclosure further relates to nucleic acid constructs, such as vectors and expression cassettes, containing a regulatory element operably linked to a heterologous nucleotide sequence. The disclosure further relates to methods for stably transforming a host cell, such as a labyrinthulomycetes cell with transgenes. Stably transformed recombinant cells, progeny, biomaterials derived therefrom, and methods for preparing and using the same are also provided.
摘要翻译：本公开通常涉及用于调节重组细胞如迷路形细胞核细胞中的基因表达的新型多核苷酸分子。本公开还涉及含有可操作地连接到异源核苷酸序列的调节元件的核酸构建体，例如载体和表达盒。本公开还涉及用转基因稳定转化宿主细胞如迷路形细胞细胞的方法。还提供了稳定转化的重组细胞，后代，从其衍生的生物材料及其制备和使用方法。

2. 发明申请

WO2019173226A1 ORGANISMS AND METHODS FOR PRODUCING GLYCOMOLECULES WITH LOW SULFATION 审中-公开
公开(公告)号：WO2019173226A1
公开(公告)日：2019-09-12
申请号：PCT/US2019/020566
申请日：2019-03-04
申请人： SYNTHETIC GENOMICS, INC.
发明人： CAIAZZA, Nicky, C. , URANO, Jun
IPC分类号： C12N9/00 , C12P21/00
摘要： The invention provides recombinant organisms containing a nucleic acid encoding a heterologous glycomolecule that has a low sulfation profile or that is unsulfated. In one embodiment the heterologous glycomolecule is an immunoglobulin molecule. The recombinant organisms have a genetic modification to at least one sulfotransferase gene, such as a deletion, disruption, or other genetic modification. The cells advantageously produce and, optionally secrete, the heterologous glycomolecule. Thus, the invention provides recombinant organisms that provide glycomolecules having a glycosylation profile that is more similar to the glycosylation profile produced in a mammalian cell, and therefore may be safer and more effective for use as a therapeutic in humans or animals. The glycomolecules can be a glycoprotein, glycopeptide, or a glycolipid.

3. 发明申请

WO2018085273A1 EXPRESSION OF MODIFIED GLYCOPROTEINS AND GLYCOPEPTIDES 审中-公开
标题翻译：修饰的糖蛋白和糖肽的表达
公开(公告)号：WO2018085273A1
公开(公告)日：2018-05-11
申请号：PCT/US2017/059304
申请日：2017-10-31
申请人： SYNTHETIC GENOMICS, INC.
发明人： CAIAZZA, Nicky, C.
IPC分类号： C12N1/19 , C12N15/09 , C12N15/80 , C12P21/00
摘要： The present invention provides recombinant cells that contain a modification causing altered expression or function of at least one mannosyl transferase enzyme. As a result of the modification the cells produce a glycoprotein or glycopeptide that has an N-linked glycan profile that is simplified or humanized. The glycoprotein or glycopeptide can have at least 25% fewer high mannose structures on than the glycoprotein or glycopeptide produced by a cell that does not have the modification. In some embodiments the modification is a deletion, knock out, or disruption of a gene encoding a mannosyl transferase, which can be in an Alg3 gene. Therefore, the proteins produced avoid many of the problems associated with the therapeutic use of glycoproteins from species having foreign or plant-like patterns of glycosylation. The invention also provides compositions of the glycoproteins or glycopeptides and methods of making them.
摘要翻译：本发明提供了含有引起至少一种甘露糖基转移酶的表达或功能改变的修饰的重组细胞。作为修饰的结果，细胞产生具有简化或人源化的N-连接的聚糖谱的糖蛋白或糖肽。糖蛋白或糖肽可以具有比不具有修饰的细胞产生的糖蛋白或糖肽少至少25％的高甘露糖结构。在一些实施方案中，修饰是编码甘露糖基转移酶的基因的缺失，敲除或破坏，所述甘露糖基转移酶可位于Alg3基因中。因此，所产生的蛋白质避免了许多与具有外来或植物样糖基化模式的物种的糖蛋白的治疗用途有关的问题。本发明还提供了糖蛋白或糖肽的组合物及其制备方法。

4. 发明申请

WO2014093535A1 PEG-MEDIATED ASSEMBLY OF NUCLEIC ACID MOLECULES 审中-公开
标题翻译： PEG介导的核酸分子组装
公开(公告)号：WO2014093535A1
公开(公告)日：2014-06-19
申请号：PCT/US2013/074471
申请日：2013-12-11
申请人： SYNTHETIC GENOMICS, INC.
发明人： URANO, Jun , GIBSON, Daniel, G. , CAIAZZA, Nicky, C. , QI, Zhiqing
IPC分类号： C12P19/34 , C12Q1/68 , C12N15/00
CPC分类号： C12P19/34 , C12N15/1027 , C12N15/1031 , C12Q1/6844 , C12Q2521/101 , C12Q2527/101 , C12Q2527/125 , C12Q2527/143 , C12Q2533/107
摘要： The present invention discloses methods for assembling a nucleic acid molecule from a set of overlapping oligonucleotides. The method involves contacting a set of overlapping oligonucleotides with a DNA polymerase, a mixture of dNTPs, and a crowding agent to form an assembly mixture. In one embodiment the crowding agent is polyethylene glycol (PEG). The presence of the crowding agent facilitates the nucleic acid assembly process of the invention. The assembly mixture is then subjected to multiple cycles, each cycle comprising an annealing phase, an extension phase, and a denaturation phase, and the desired nucleic acid molecule is thereby assembled. In some embodiments one or more of the phases are time varied.
摘要翻译：本发明公开了从一组重叠寡核苷酸组装核酸分子的方法。该方法包括将一组重叠的寡核苷酸与DNA聚合酶，dNTP的混合物和拥挤剂接触以形成组合混合物。在一个实施方案中，拥挤剂是聚乙二醇（PEG）。拥挤剂的存在促进了本发明的核酸组装过程。然后将组合混合物进行多个循环，每个循环包含退火阶段，延伸相和变性阶段，从而组装所需的核酸分子。在一些实施方案中，一个或多个相是时间变化的。

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