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1. 发明申请

WO2013058654A2 METHODS FOR PRODUCING CINNAMOLIDE AND/OR DRIMENDIOL 审中-公开
标题翻译：生产新戊酰胺和/或维生素B1的方法
公开(公告)号：WO2013058654A2
公开(公告)日：2013-04-25
申请号：PCT/NL2012/050729
申请日：2012-10-19
申请人： KEYGENE N.V.
发明人： BOUWMEESTER, Hendrik Jan , HENQUET, Maurice Gerard Leon , JONGSMA, Maarten Anthonie
IPC分类号： C12N9/00
CPC分类号： C12P7/02 , C12N9/0004 , C12N9/0006 , C12N9/16 , C12N15/8241 , C12N15/8286 , C12P17/04
摘要： Described is a nucleic acid sequence isolated from Persicaria hydropiper and encoding a drimenol oxidase protein, expression vectors comprising such nucleic acid sequence, chimeric genes comprising such nucleic acid sequence, host cells or host organisms altered to harbour the drimenol oxidase nucleic acid sequence, and the drimenol oxidase protein itself. Methods for producing cinnamolide and/or drimendiol and/or enhanced levels of cinnamolide and/or drimendiol, in a cell or organism harbouring such nucleic acid sequence are provided. Transgenic organisms comprising the nucleic acid sequence or a chimeric gene of the invention are also provided. The present invention especially relates to transgenic plants with enhanced resistance to insects and enhanced insect antifeedant properties.
摘要翻译：描述了从Persicaria hydropiper分离的核酸序列，并编码一种石蜡氧化酶蛋白，包含该核酸序列的表达载体，包含该核酸序列的嵌合基因，被改变为含有该二醇氧化酶核酸序列的宿主细胞或宿主生物，以及石蜡氧化酶蛋白本身。提供了在含有这种核酸序列的细胞或生物体中生产肉桂酸酯和/或烈im醇和/或增强的肉桂酸酯和/或烈end醇水平的方法。还提供了包含本发明的核酸序列或嵌合基因的转基因生物。本发明特别涉及具有增强的对昆虫的抗性和增强的昆虫抗食性质的转基因植物。

2. 发明申请

WO2013058655A1 METHODS AND COMPOSITIONS FOR PRODUCING DRIMENOL 审中-公开
标题翻译：用于生产蛇毒酚的方法和组合物
公开(公告)号：WO2013058655A1
公开(公告)日：2013-04-25
申请号：PCT/NL2012/050730
申请日：2012-10-19
申请人： KEYGENE N.V.
发明人： BOUWMEESTER, Hendrik Jan , HENQUET, Maurice Gerard Leon , JONGSMA, Maarten Anthonie
IPC分类号： C12N9/16 , C12N15/82
CPC分类号： C12P7/02 , C12N9/0004 , C12N9/0006 , C12N9/16 , C12N15/8241 , C12N15/8286 , C12P17/04
摘要： The present invention relates to nucleic acids sequences derived from Valeriana officinalis and/or Persicaria hydropiper and encoding drimenol synthase polypeptides. The present invention also provides the amino acid sequences of the polypeptides. The invention further provides host cells or organisms genetically modified to harbour the polynucleotides of the invention. A method to produce drimenol and/or a drimenol derivative by contacting farnesyl diphosphate with a polypeptide having a drimenol synthase activity is also part of this invention.
摘要翻译：本发明涉及源自缬草和/或Persicaria hydropiper的核酸序列，并编码檀香醇合成酶多肽。本发明还提供了多肽的氨基酸序列。本发明还提供遗传修饰以携带本发明的多核苷酸的宿主细胞或生物体。通过使法呢基二磷酸酯与具有萜醇合成酶活性的多肽接触来生产蓖麻醇和/或dr醇衍生物的方法也是本发明的一部分。

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