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    • 1. 发明申请
    • PLANT GROWTH REGULATING GENES, PROTEINS AND USES THEREOF
    • 植物生长调节基因,蛋白质及其用途
    • WO02083901A3
    • 2003-08-21
    • PCT/EP0204035
    • 2002-04-11
    • CROPDESIGN NVSAUTER MARGRETLORBIECKE RENEMIRONOV VLADIMIRFRANKARD VALERIEDILLEN WILLYLEJEUNE PIERREBROEKAERT WILLEM
    • SAUTER MARGRETLORBIECKE RENEMIRONOV VLADIMIRFRANKARD VALERIEDILLEN WILLYLEJEUNE PIERREBROEKAERT WILLEM
    • C07K14/415C12N15/29C12N15/82A01H5/00C07K16/16C12Q1/68
    • C12N15/8262C07K14/415C12N15/8237C12N15/8261Y02A40/146
    • Provided are methods for controlling or altering the growth characteristics of a plant or of an organ or tissue or cell of this plant. This method comprises introduction and/or expression of one or more Growth Regulating Proteins functional in a plant or parts thereof and/or one or more DNA sequences encoding such proteins. Another method comprises contacting a plant or plant tissue with the purified Growth Regulated Protein or the active product derived from it. Provided are also DNA sequences encoding plant Growth Regulating Protein(s) as well as methods for obtaining the same. Furthermore, vectors comprising said DNA sequences are described, wherein the DNA sequences are operatively linked to regulatory elements allowing expression in prokaryotic and/or eukaryotic host cells. In addition, proteins encoded by said DNA sequences, antibodies to said proteins and methods for their production are provided. Furthermore, regulatory sequences, which naturally regulate the expression of the above-described DNA sequences, are described. Methods for the identification of compounds being capable of interacting with said Growth Regulating Proteins are described as well. Furthermore, transgenic plant cells, plant tissues and plants containing the above-described DNA sequences and vectors are described, as well as the use of the aforementioned DNA sequences, vectors, proteins, antibodies, regulatory sequences and/or compounds identified by the method of the invention in plant cell and tissue culture, plant breeding and/or agriculture.
    • 提供了用于控制或改变植物或该植物的器官或组织或细胞的生长特性的方法。 该方法包括在植物或其部分和/或编码这种蛋白质的一个或多个DNA序列中引入和/或表达一种或多种功能的生长调节蛋白。 另一种方法包括使植物或植物组织与纯化的生长调节蛋白或其衍生的活性产物接触。 还提供编码植物生长调节蛋白的DNA序列以及获得其的方法。 此外,描述了包含所述DNA序列的载体,其中所述DNA序列可操作地连接到允许在原核和/或真核宿主细胞中表达的调节元件。 此外,提供了由所述DNA序列编码的蛋白质,对所述蛋白质的抗体及其生产方法。 此外,描述了调节序列,其自然调节上述DNA序列的表达。 还描述了用于鉴定能够与所述生长调节蛋白相互作用的化合物的方法。 此外,描述了含有上述DNA序列和载体的转基因植物细胞,植物组织和植物,以及使用上述DNA序列,载体,蛋白质,抗体,调节序列和/或化合物 本发明在植物细胞和组织培养,植物育种和/或农业中。