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    • 9. 发明授权
    • Fluorescent fatty acids derived from dipyrrometheneboron difluoride dyes
    • 衍生自二吡咯亚甲基二氟化物染料的荧光脂肪酸
    • US5338854A
    • 1994-08-16
    • US654881
    • 1991-02-13
    • Hee C. KangRichard P. Haugland
    • Hee C. KangRichard P. Haugland
    • C07D207/44C07F5/02H01S3/213H01S3/20
    • C07D207/44C07F5/022H01S3/213
    • This invention relates to improved fluorescent fatty acid analogs derived from dipyrrometheneboron difluoride ("BDY") dyes that absorb light at wavelengths longer than 480 nm. The BDY fluorophore or a derivative thereof is incorporated at various locations along the alkyl portion of the fatty acid ("BDY fatty acid"). The general formula of a BDY fatty acid is as follows: ##STR1## wherein at least one of the substituents R.sub.1, R.sub.2, R.sub.3, R.sub.4, R.sub.5, R.sub.6 and R.sub.7 is a carboxylic acid terminated alkyl residue containing 5 to 22 carbon atoms. The remaining substituents, singly or in any combination, are: additional alkyl or arylalkyl residues containing 1 to 16 aliphatic carbon atoms, or aryl or heteroaryl residues, or hydrogen. Symmetrically substituted BDY fluorophores are conveniently synthesized from a single pyrrole precursor in a "one-pot" reaction. Alternatively, BDY fatty acids are synthesized from two different pyrrole precursors. The combination of fatty acids with these electrically neutral, photostable, strongly colored, and mostly highly fluorescent BDY dyes having relatively narrow absorption and emission spectra results in an improved fluorescent probe particularly useful for studying natural and synthetic lipid membranes and related areas.
    • 本发明涉及衍生自吸收长于480nm波长的光的二吡咯亚甲基二氟化二(“BDY”)染料的改进的荧光脂肪酸类似物。 BDY荧光团或其衍生物沿着脂肪酸(“BDY脂肪酸”)的烷基部分的不同位置被并入。 BDY脂肪酸的通式如下:其中至少一个取代基R 1,R 2,R 3,R 4,R 5,R 6和R 7是羧酸封端的含5-22个碳原子的烷基。 剩余的取代基,单独或以任何组合是:含有1至16个脂族碳原子的另外的烷基或芳基烷基,或芳基或杂芳基残基,或氢。 对称取代的BDY荧光团在“一锅”反应中方便地从单一吡咯前体合成。 或者,BDY脂肪酸由两种不同的吡咯前体合成。 脂肪酸与具有相对较窄吸收和发射光谱的这些电中性,光稳定,强烈着色和大部分高度荧光的BDY染料的组合导致改进的荧光探针,其特别用于研究天然和合成脂质膜及相关区域。