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1. 发明授权

US06270984B1 Modulating robo: ligand interactions 失效
标题翻译：调节robo：配体相互作用
公开(公告)号：US06270984B1
公开(公告)日：2001-08-07
申请号：US09540245
申请日：2000-03-31
申请人： Corey S. Goodman , Thomas Kidd , Katja Brose , Marc Tessier-Lavigne
发明人： Corey S. Goodman , Thomas Kidd , Katja Brose , Marc Tessier-Lavigne
IPC分类号： G01N3383
CPC分类号： C07K14/70503 , A61K38/00 , C07K14/4702 , G01N33/566 , G01N33/68 , G01N2333/43573 , G01N2500/02
摘要： Disclosed are methods and compositions for identifying agents which modulate the interaction of Robo and a Robo ligand and for modulating the interaction of Robo and a Robo ligand. The methods for identifying Robo:ligand modulators find particular application in commercial drug screens. These methods generally comprise (1) combining a Robo polypeptide, a Slit polypeptide and a candidate agent under conditions whereby, but for the presence of the agent, the Robo and Slit polypeptides engage in a first interaction, and (2) determining a second interaction of the Robo and Slit polypeptides in the presence of the agent, wherein a difference between the first and second interactions indicates that the agent modulates the interaction of the Robo and Slit polypeptides. The subject methods of modulating the interaction of Robo and a Robo ligand involve combining a Robo polypeptide, a Slit polypeptide and a modulator under conditions whereby, but for the presence of the modulator, the Robo and Slit polypeptides engage in a first interaction, whereby the Robo and Slit polypeptides engage in a second interaction different from the first interaction. In a particular embodiment, the modulator is dominant negative form of the Robo or Slit polypeptide.
摘要翻译：公开了用于鉴定调节Robo和Robo配体的相互作用并调节Robo和Robo配体的相互作用的试剂的方法和组合物。用于鉴定Robo：配体调节剂的方法在商业药物筛选中发现具体应用。这些方法通常包括（1）在条件下组合Robo多肽，狭缝多肽和候选试剂，但是对于所述试剂的存在，所述Robo和狭缝多肽参与第一相互作用，和（2）确定第二相互作用的Robo和Slit多肽，其中所述第一和第二相互作用之间的差异指示所述试剂调节所述Robo和Slit多肽的相互作用。调节Robo和Robo配体的相互作用的主题方法包括在条件下组合Robo多肽，狭缝多肽和调节剂，但是对于调节剂的存在，Robo和Slit多肽参与第一相互作用，由此 Robo和Slit多肽参与与第一相互作用不同的第二相互作用。在一个具体实施方案中，调节剂是Robo或Slit多肽的显性阴性形式。

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