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1. 发明授权

US5834299A Method for dehairing of hides or skins by means of enzymes 失效
标题翻译：通过酶对皮革或皮肤进行脱毛的方法
公开(公告)号：US5834299A
公开(公告)日：1998-11-10
申请号：US878910
申请日：1997-06-19
申请人： Lars Peter Andersen
发明人： Lars Peter Andersen
IPC分类号： C12S7/00 , C14C1/06 , C14C1/00
CPC分类号： C14C1/065
摘要： The invention relates to an environmentally friendly method for dehairing of hides or skins by means of enzymes comprising the following steps: 1) the hides or skins are soaked, 2) the soaked hides or skins are subjected to a main soak, and 3) the thus treated hides or skins are dehaired by addition of water, exposure to mechanical influence and subjection to at least one protease, characterized in that the hides or skins are subjected to at least one protein disulfide redox agent at least one time during step 1) to 3).
摘要翻译： PCT No.PCT / DK95 / 00509 Sec。 371日期：1997年6月19日 102（e）日期1997年6月19日PCT 1995年12月20日PCT PCT。出版物WO96 / 日期1996年6月27日本发明涉及一种通过酶对皮革或皮肤进行脱毛的环保方法，包括以下步骤：1）将皮革或皮肤浸泡，2）浸泡的皮革或皮肤经受主浸泡，和3）如此处理的皮革或皮肤通过加入水，暴露于机械影响并且服用于至少一种蛋白酶而脱毛，其特征在于，皮革或皮肤经受至少一种蛋白质二硫键氧化还原剂至少一次在步骤1）至3）。

2. 发明授权

US5271912A Enzymatic processing of materials containing chromium and protein 失效
标题翻译：含铬和蛋白质的材料的酶加工
公开(公告)号：US5271912A
公开(公告)日：1993-12-21
申请号：US848775
申请日：1992-03-10
申请人： Maryann M. Taylor , Edward J. Diefendorf , Willian N. Marmer , Eleanor M. Brown
发明人： Maryann M. Taylor , Edward J. Diefendorf , Willian N. Marmer , Eleanor M. Brown
IPC分类号： C01G37/00 , C02F3/34 , C12P21/06 , C12S7/00 , C14C3/32 , C22B34/32 , C12J7/00
CPC分类号： C02F3/342 , C01G37/00 , C12P21/06 , C14C3/32 , C22B34/32 , C02F2103/24 , Y02W30/76 , Y10S210/913
摘要： Chromium containing protein materials are treated by processes which include the steps of: combining the material to be treated with sufficient water to effect the solubilization of the gelable protein recovered therefrom, and an alkali additive. The mixture is thereafter held at a temperature of from about 60.degree. C. to about 85.degree. C. for a period of time of from about 0.5 hour to about 8 hours. The mixture is then filtered to remove the aqueous phase, which contains solubilized gelable protein. Subsequently, water is again added to the resultant chrome-containing proteinaceous cake and at least one enzyme is utilized to hydrolyze the remaining protein thus producing a product containing solubilized partially hydrolyzed protein and insoluble chromium. After separation, the chrome in the insoluble residue may be recycled into the pickling or tanning process, and the essentially chrome-free protein components may be used as ingredients in feeds, fertilizers, and cosmetics. The processes of the present invention are used to particular advantage to treat wastes from the leather tanning industry including blue stock, chrome shavings, chrome buffing dust, chrome cakes and chrome sludges.
摘要翻译：含铬蛋白质材料通过包括以下步骤的方法处理：将待处理的材料与足够的水组合以实现从其回收的可胶凝蛋白的溶解，以及碱添加剂。然后将混合物在约60℃至约85℃的温度下保持约0.5小时至约8小时的时间。然后过滤混合物以除去含有溶解的可胶凝蛋白的水相。随后，再向所得含铬蛋白质滤饼中加入水，并使用至少一种酶来水解剩余的蛋白质，从而产生含有溶解的部分水解蛋白质和不溶性铬的产物。分离后，不溶残留物中的铬可以再循环到酸洗或鞣制过程中，并且基本上不含铬的蛋白质组分可以用作饲料，肥料和化妆品中的成分。本发明的方法特别有利于处理皮革制革行业的废物，包括蓝色原料，铬屑，铬抛光粉，铬蛋糕和铬泥。

3. 发明申请

US20130105100A1 Method And Composition For Enzymatic Treatment Of Fiber For Papermaking, And Paper Products Made Therewith 有权
标题翻译：用于造纸纤维的酶法处理方法和组合物，以及与此相关的纸制品
公开(公告)号：US20130105100A1
公开(公告)日：2013-05-02
申请号：US13661334
申请日：2012-10-26
申请人： Buckman Laboratories International, Inc.
发明人： Stephen D. Bryant , Kevin J. MacDonald , Bernard Janse , Xiangdong Zhou , Philip Hoekstra , Daniel E. Glover
IPC分类号： D21C9/16 , C12N9/96 , C12S7/00 , D21H27/00
CPC分类号： D21C9/123 , D21C5/005 , D21C9/086 , D21C9/1005 , D21C9/1036
摘要： A method is provided for controlling organic contaminants, such as xylans, pitch or both, that interfere with bleaching of fibers and/or cause other interference(s) in papermaking systems. The method includes contacting fibers before any bleaching thereof with a composition which contains a hemicellulolytic enzyme and an organic contaminant removal adjuvant to liberate the organic contaminants from the fibers. The treated fibers can then be bleached and further used, for example, in making paper. The present invention also relates to the treatment compositions and to paper products made with fiber materials treated with these compositions. A method of enhancing enzymatic degradation of a substrate as well as formulations and systems for achieving the same are also provided. Various substrates can be degraded or otherwise processed, including biomass, paper mill sludge, and animal hides. Enzymatic degradation can be enhanced by including one or more polymeric surfactants.
摘要翻译：提供了一种用于控制干扰纤维漂白和/或在造纸系统中造成其它干扰的有机污染物如木聚糖，沥青或两者的方法。该方法包括使纤维在其任何漂白之前与含有半纤维素分解酶和有机污染物去除佐剂的组合物接触以释放纤维中的有机污染物。然后处理的纤维可以被漂白并进一步用于例如制造纸。本发明还涉及用这些组合物处理的纤维材料制成的处理组合物和纸制品。还提供了增强底物的酶降解的方法以及用于实现其的制剂和体系。可以降解或处理各种底物，包括生物质，造纸厂污泥和动物皮。通过包括一种或多种聚合物表面活性剂可以提高酶降解。

4. 发明授权

US5508195A Method for liming hides and skins 失效
标题翻译：鞣皮和皮肤的方法
公开(公告)号：US5508195A
公开(公告)日：1996-04-16
申请号：US533674
申请日：1995-09-26
申请人： Juergen Christner , Tilman Taeger , Gertrud Wick
发明人： Juergen Christner , Tilman Taeger , Gertrud Wick
IPC分类号： C12S7/00 , C14C1/06 , C12S3/00 , C12N9/54 , C14C1/08
CPC分类号： C14C1/065
摘要： A method for liming hides and skins using a proteolytic enzyme in an aqueous-alkaline float is taught, wherein the liming float has a pH value from 10-14 and contains both thiourea dioxide and an alkaline protease having elastase activity.
摘要翻译：教导了一种在水性碱性漂浮液中使用蛋白水解酶处理皮肤和皮肤的方法，其中柠檬浮游物的pH值为10-14，并含有硫脲二氧化物和具有弹性蛋白酶活性的碱性蛋白酶。

5. 发明申请

US20120142073A1 HIDE PROCESSING METHODS AND COMPOSITIONS 有权
标题翻译：隐藏处理方法和组合
公开(公告)号：US20120142073A1
公开(公告)日：2012-06-07
申请号：US13321316
申请日：2010-11-16
申请人： Shaon Ray Chaudhuri
发明人： Shaon Ray Chaudhuri
IPC分类号： C14C1/06 , C12N1/20 , C12N9/20 , C12S7/00
CPC分类号： C14C1/065 , C12N9/20 , C12N9/52 , C12R1/385
摘要： Provided in part herein are compositions that include isolated microbial enzymes, such as proteases, lipases, useful in dehairing leather. Also provided are methods for isolating proteases, lipases, from microbes, as well as methods of reusing these enzymes effectively for several cycles in an environment friendly process.
摘要翻译：本文部分提供了包含分离的微生物酶的组合物，例如蛋白酶，脂肪酶，用于脱皮皮革。还提供了用于从微生物中分离蛋白酶，脂肪酶的方法，以及在环境友好过程中多次循环使用这些酶的方法。

6. 发明申请

US20050026269A1 Novel alkaline protease variants and detergents and cleaning agents containing said novel alkaline protease variants 审中-公开
标题翻译：含有所述新型碱性蛋白酶变体的新型碱性蛋白酶变体和洗涤剂和清洁剂
公开(公告)号：US20050026269A1
公开(公告)日：2005-02-03
申请号：US10476463
申请日：2002-04-24
申请人： Beatrix Kottwitz , Karl-Heinz Maurer , Roland Breves
发明人： Beatrix Kottwitz , Karl-Heinz Maurer , Roland Breves
IPC分类号： A23K1/165 , A23C19/032 , A23L1/00 , A61K8/00 , A61K8/64 , A61K8/66 , A61K8/96 , A61Q5/02 , A61Q19/10 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/54 , C12N15/09 , C12N15/31 , C12N15/57 , C12P21/06 , C12R1/07 , C12S7/00 , C12S9/00 , C12S11/00 , G03C11/24 , C07H21/04 , C12N9/56 , C12N15/74
CPC分类号： C12N9/54
摘要： Described herein are novel alkaline protease variants derived from subtilisin. These variants have, with respect to the amino acid sequence of Bacillus lentus subtilisin, variations at amino acid positions 199 and 211, and at least one modification that contributes to the stabilization of the molecule, the modification preferably being variations at amino acid positions 3 and/or 4. Preferably, the variant is B. lentus alkaline protease S3T/NV4I/V199I/L211G. Also described are detergents and cleaning agents comprising the novel alkaline protease variants. Methods of use employing the novel alkaline protease variants are also described.
摘要翻译：本文描述了衍生自枯草杆菌蛋白酶的新型碱性蛋白酶变体。这些变体具有关于迟缓芽孢杆菌枯草杆菌蛋白酶的氨基酸序列，氨基酸位置199和211的变化以及有助于分子稳定化的至少一种修饰，优选在氨基酸位置3和/ /或4.优选地，变体是枯草芽孢杆菌碱性蛋白酶S3T / NV4I / V199I / L211G。还描述了包含新型碱性蛋白酶变体的洗涤剂和清洁剂。还描述了使用新型碱性蛋白酶变体的使用方法。

7. 发明授权

US5710040A Stable enzymatic aqueous liquid composition for the production of leather 失效
标题翻译：稳定的酶水性液体组合物用于生产皮革
公开(公告)号：US5710040A
公开(公告)日：1998-01-20
申请号：US600735
申请日：1996-02-13
申请人： Juergen Christner , Gertrud Wick
发明人： Juergen Christner , Gertrud Wick
IPC分类号： C12S7/00 , C14C1/00 , C14C1/04 , C14C1/06 , C14C1/08
CPC分类号： C14C1/00
摘要： An aqueous liquid composition containing one or more enzymatic active substances and at least 10 wt % to a maximum of (100-x) wt % molasses, wherein x is the fraction of enzymatic active substances in wt % and wherein x is a value from 0.001 to 90, which is useful for the production of leather in the beamhouse, to improve rehydration and dirt removal while soaking, to improve the loosening of hair and to inhibit swelling during liming, and to improve the cleaning of the surface of the skin during bating.
摘要翻译：含有一种或多种酶活性物质和至少10重量％至最大（100-x）重量％糖蜜的含水液体组合物，其中x是以重量％计的酶活性物质的分数，其中x为0.001 至90，其可用于在梁屋中生产皮革，以在浸泡期间改善补液和除污，以改善头发的松动并抑制跛行期间的肿胀，以及改善皮肤表面的清洁。

8. 发明授权

US5094946A Enzymatic processing of materials containing chromium and protein 失效
标题翻译：含铬和蛋白质的材料的酶加工
公开(公告)号：US5094946A
公开(公告)日：1992-03-10
申请号：US476843
申请日：1990-02-08
申请人： Maryann M. Taylor , Edward J. Diefendorf , George C. Na , William N. Marmer
发明人： Maryann M. Taylor , Edward J. Diefendorf , George C. Na , William N. Marmer
IPC分类号： C02F3/34 , C12P21/06 , C12S7/00
CPC分类号： C02F3/342 , C12P21/06 , C02F2103/24 , Y10S210/913
摘要： Materials containing both chromium and protein and treated by processes which include the steps of: combining a material to be treated (i.e. containing both chromium and protein) with sufficient water to produce a mixture having from about 75 wt. % water to about 95 wt. % water, and an additive which both provides an alkaline pH and provides calcium or magnesium as an enzyme cofactor. The mixture is thereafter held at a temperature of from about 60.degree. C. to about 75.degree. C. for a period of time of from about 0.5 hour to about 4 hours. Subsequently, at least one enzyme is utilized to hydrolyze the protein, thus producing a product containing solubilized hydrolyzed protein and insoluble chromium. After separation of the soluble fraction from the insoluble fraction, the chrome in the insoluble residue may be recycled into the pickling or tanning process, and the essentially chrome-free protein hydrolyzate is potentially useful as an ingredient in feeds, fertilizers, and cosmetics. The processes of the present invention are used to particular advantage to treat wastes from the leather tanning industry, for example, material such as blue stock, chrome shavings, chrome buffing dust, chrome cakes and chrome sludges.
摘要翻译：含有铬和蛋白质并且通过方法处理的材料包括以下步骤：将待处理材料（即含有铬和蛋白质）与足够的水组合以产生具有约75wt。％水至约95wt。％的水和添加剂，其都提供碱性pH并提供钙或镁作为酶辅因子。然后将混合物在约60℃至约75℃的温度下保持约0.5小时至约4小时的时间。随后，使用至少一种酶水解蛋白质，从而产生含有溶解的水解蛋白质和不溶性铬的产品。在将可溶性级分与不溶性级分分离后，不溶残留物中的铬可以再循环到酸洗或鞣制过程中，并且基本上无铬的蛋白质水解产物作为饲料，肥料和化妆品中的成分潜在地有用。本发明的方法特别有利于处理皮革制革工业中的废物，例如蓝色原料，铬屑，铬抛光粉，铬饼和铬泥等材料。

9. 发明授权

US5089414A Enzymatic soaking method 失效
标题翻译： ENZYMATIC SOAKING方法
公开(公告)号：US5089414A
公开(公告)日：1992-02-18
申请号：US528717
申请日：1990-05-24
申请人： Juergen Christner , Ernst Pfleiderer , Tilman Taeger
发明人： Juergen Christner , Ernst Pfleiderer , Tilman Taeger
IPC分类号： C12S7/00 , C14C1/04
CPC分类号： C14C1/04
摘要： The soaking of hides and skins is enzymatically assisted in a method using an aqueous float having a pH from 9 to 11 which containsA) a lipase having an activity optimum in the pH region from 9 to 11,B) a protease with activity in the pH region from 9 to 11, andC) a surface active agent.
摘要翻译：皮肤和皮肤的浸泡在使用pH为9-11的水性漂浮物的方法中酶促辅助，其含有A）在pH范围为9至11的活性最佳的脂肪酶，B）具有活性的蛋白酶 pH范围为9至11，和C）表面活性剂。

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