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1. 发明申请

US20190079089A1 METHOD FOR DETECTING STAPHYLOCOCCUS CONTAINED IN MILK 审中-公开
公开(公告)号：US20190079089A1
公开(公告)日：2019-03-14
申请号：US16184384
申请日：2018-11-08
申请人： ASAHI KASEI KABUSHIKI KAISHA
发明人： Koji MAEHANA , Kenji MATSUYAMA
IPC分类号： G01N33/569 , C12N9/52 , G01N33/558 , C12Q1/14
CPC分类号： G01N33/56938 , C12N9/52 , C12Q1/14 , C12Y304/24075 , G01N33/558 , G01N2333/31 , G01N2333/952 , G01N2333/96486
摘要： The object is to provide a lysis method, lysis treatment solution, detection method using an immunochromatographic device, and detection kit comprising an immunochromatographic device for detecting whether causative bacterium of mastitis is a staphylococcus or not by using milk of a livestock animal. There is provided a method for lysing a staphylococcus, which comprises the step of mixing a lysis agent containing a lytic enzyme, and at least one kind of ampholytic surfactant, and preferably further containing at least one kind of nonionic surfactant, with milk obtained form a livestock animal to lyse a staphylococcus existing in the milk. The lytic enzyme is preferably lysostaphin.

2. 发明申请

US20180251812A1 COMPOSITIONS AND METHODS FOR IMPROVED NEUROTOXIN ASSAYS 审中-公开
公开(公告)号：US20180251812A1
公开(公告)日：2018-09-06
申请号：US15980454
申请日：2018-05-15
申请人： BIOMADISON, INC.
发明人： Robert D. Fish , Min Dong
IPC分类号： C12Q1/37 , G01N33/542 , C07K14/00 , C07K14/47 , C07K19/00 , G01N21/64 , G01N33/94
CPC分类号： C12Q1/37 , C07K14/00 , C07K14/47 , C07K19/00 , C07K2319/50 , C07K2319/60 , G01N21/6428 , G01N33/542 , G01N33/9406 , G01N2021/6432 , G01N2333/952
摘要： Methods for improving the specificity of assays for botulinum neurotoxins are described. Reporting constructs are provided that include cleavable linker sequence with genetically modified recognition and/or cleavage sites. These linker sequences act as a substrate for a botulinum neurotoxin, with cleavage of the reporting construct providing a detectable signal. When first and second neurotoxins have activity with the same substrate protein, mutation and/or deletion of a recognition and/or cleavage site associated with the second neurotoxin improves the specificity of the detectable signal for the first neurotoxin.

3. 发明授权

US09952198B2 Translocation of a polymer through a nanopore 有权
公开(公告)号：US09952198B2
公开(公告)日：2018-04-24
申请号：US14773072
申请日：2014-03-05
申请人： ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY
发明人： Stuart Lindsay , Peiming Zhang
IPC分类号： G01N33/68 , G01N33/487 , G01N27/447 , G01N27/26 , G01N33/543 , G01N15/12 , C12Q1/37
CPC分类号： G01N33/48721 , C12Q1/37 , G01N15/12 , G01N27/44704 , G01N27/44747 , G01N27/44756 , G01N33/5438 , G01N33/6803 , G01N33/6848 , G01N2333/952 , G01N2570/00 , Y10T436/255
摘要： Embodiments of the present disclosure are directed to methods, systems and devices, for analyzing the molecules. For example, in some embodiments, a system is provided which includes a first volume of conducting fluid, a second volume of conducting fluid, an orifice in communication with the first and second volumes of fluid, and means for applying an electric potential difference between the first and second volumes of fluid. In some embodiments, a conjugate product is provided which includes charged polymers each having attached thereto at least one first molecule for analysis, where the product carries a predetermined charge greater than the charge on the first molecule, and upon dissolving a product in the first volume of fluid, the product is directed into the orifice.

4. 发明授权

US09816992B2 Compositions and methods relating to Lyme disease 有权
公开(公告)号：US09816992B2
公开(公告)日：2017-11-14
申请号：US15200657
申请日：2016-07-01
申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services
发明人： Theresa M. Russell , Barbara J. B. Johnson
IPC分类号： G01N33/573 , G01N33/58 , G01N33/68 , G01N33/569 , C07K16/12
CPC分类号： G01N33/573 , C07K16/12 , C07K2317/32 , G01N33/56911 , G01N33/581 , G01N33/6863 , G01N33/6869 , G01N2333/20 , G01N2333/952 , G01N2469/10 , G01N2469/20 , G01N2800/26 , G01N2800/52 , Y02A50/57
摘要： Compositions and methods of the present invention relating to B. burgdorferi HtrA sensu lato (BbHtrA) protease activity, its substrates, cleavage products, biological effects and use in detection, diagnosis and/or treatment of Lyme disease are provided.

5. 发明授权

US09651553B2 Immuno-based retargeted endopeptidase activity assays 有权
公开(公告)号：US09651553B2
公开(公告)日：2017-05-16
申请号：US14695938
申请日：2015-04-24
申请人： Allergan, Inc.
发明人： Joanne Wang , Hong Zhu , Dianne D. Hodges , Ester Fernandez-Salas
IPC分类号： C12N9/00 , C12Q1/37 , G01N33/573 , G01N33/50 , G01N33/53 , C12N9/50 , C07K16/18
CPC分类号： G01N33/573 , C07K16/18 , C07K2317/34 , C07K2317/56 , C07K2317/92 , C12N9/50 , C12Q1/37 , C12Y304/24069 , G01N33/5014 , G01N33/5035 , G01N33/53 , G01N2333/33 , G01N2333/47 , G01N2333/952 , G01N2333/96425
摘要： The present specification discloses a retargeted endopeptidase pharmaceutical wherein the activity has been determined by the methods disclosed.

6. 发明申请

US20160202245A1 Means and Methods for the determination of the biological activity of Neurotoxin polypeptides in cells 审中-公开
标题翻译：用于测定细胞中神经毒素多肽的生物学活性的方法和方法
公开(公告)号：US20160202245A1
公开(公告)日：2016-07-14
申请号：US14901123
申请日：2014-06-26
申请人： MERZ PHARMA GMBH & CO. KGAA
发明人： Cornelia BRÜNN
IPC分类号： G01N33/50 , G01N21/64
CPC分类号： C07K16/18 , C07K2317/34 , G01N21/6428 , G01N33/5014 , G01N33/5058 , G01N33/5073 , G01N2021/6441 , G01N2333/33 , G01N2333/952
摘要： The present invention pertains to a method for directly determining the biological activity of a Neurotoxin polypeptide in cells, comprising the steps of: a) incubating cells susceptible to Neurotoxin intoxication with a Neurotoxin polypeptide for a time and under conditions which allow for the Neurotoxin polypeptide to exert its biological activity; b) fixing the cells and, optionally, permeabilizing the cells with a detergent; c) contacting the cells with at least a first capture antibody specifically binding to the non-cleaved and Neurotoxin-cleaved substrate and with at least a second capture antibody specifically binding to the cleavage site of the Neurotoxin-cleaved substrate, under conditions which allow for binding of said capture antibodies to said substrates; d) contacting the cells with at least a first detection antibody specifically binding to the first capture antibody, under conditions which allow for binding of said first detection antibody to said first capture antibody, thus forming first detection complexes, and at least a second detection antibody specifically binding to the second capture antibody, under conditions which allow for binding of said second detection antibody to said second capture antibody, thus forming second detection complexes; e) determining the amount of the first and second detection complexes of step d), and f) calculating the amount of substrate cleaved by said Neurotoxin polypeptide in said cells by means of the second detection complexes, thereby determining the biological activity of said Neurotoxin polypeptide in said cells. The invention further provides for a kit for carrying out the method of the invention.
摘要翻译：本发明涉及直接测定神经毒素多肽在细胞中的生物学活性的方法，包括以下步骤：a）在神经毒素多肽对神经毒素中毒敏感的情况下，使神经毒素多肽在一段时间内和在允许神经毒素多肽发挥其生物活性; b）固定细胞，并且任选地用洗涤剂使细胞透化; c）在允许细胞与至少一种特异性结合未切割和神经毒素切割的底物的第一捕获抗体和至少一种特异性结合神经毒素切割的底物的切割位点的第二捕获抗体上接触细胞，所述捕获抗体与所述底物结合; d）在允许所述第一检测抗体与所述第一捕获抗体结合从而形成第一检测复合物和至少第二检测抗体的条件下，使细胞与至少一种特异性结合第一捕获抗体的第一检测抗体接触在允许所述第二检测抗体与所述第二捕获抗体结合的条件下，特异性结合第二捕捉抗体，从而形成第二检测复合物; e）确定步骤d）的第一和第二检测复合物的量，和f）通过所述第二检测复合物计算由所述神经毒素多肽在所述细胞中切割的底物的量，由此确定所述神经毒素多肽的生物学活性在所述细胞中。本发明还提供了用于实施本发明方法的试剂盒。

7. 发明申请

US20160138073A1 GLYCATED HEXAPEPTIDE OXIDASE AND USE THEREOF 有权
标题翻译：甘草酸二氢氧化酶及其用途
公开(公告)号：US20160138073A1
公开(公告)日：2016-05-19
申请号：US14898022
申请日：2014-07-07
申请人： KYOWA MEDEX CO., LTD. , KYOTO UNIVERSITY
发明人： Noriyuki OGAWA , Fumi UMEHARA , Takehide KIMURA , Kousaku MURATA , Wataru HASHIMOTO
IPC分类号： C12Q1/26 , C12Q1/37 , C12N9/06
CPC分类号： C12Q1/26 , C12N9/0006 , C12N9/0032 , C12Q1/37 , C12Y105/03 , G01N33/723 , G01N33/725 , G01N2333/805 , G01N2333/90672 , G01N2333/952
摘要： The present invention provides a protein comprising an amino acid sequence in which arginine at position 61 of a protein comprising the amino acid sequence represented by SEQ ID NO: 1 is substituted to an amino acid selected from the group consisting of glycine, alanine, valine, leucine, serine, threonine, proline, cysteine, methionine, asparagine, glutamine, and aspartic acid; and a method for measuring a glycated hemoglobin in a sample, wherein the method comprises reacting a glycated hemoglobin in a sample with a protease to produce a glycated hexapeptide, then reacting the produced glycated hexapeptide with the aforementioned protein, and measuring a substance produced or consumed by the reaction.
摘要翻译：本发明提供了包含氨基酸序列的蛋白质，其中将包含SEQ ID NO：1所示氨基酸序列的蛋白质的61位精氨酸取代为选自甘氨酸，丙氨酸，缬氨酸，亮氨酸，丝氨酸，苏氨酸，脯氨酸，半胱氨酸，甲硫氨酸，天冬酰胺，谷氨酰胺和天冬氨酸; 以及测定样品中的糖化血红蛋白的方法，其中所述方法包括使样品中的糖化血红蛋白与蛋白酶反应以产生糖化六肽，然后使产生的糖基化六肽与前述蛋白质反应，并测量产生或消耗的物质通过反应。

8. 发明申请

US20150253326A1 Methods and Compositions for Detecting Botulinum Neurotoxin 审中-公开
标题翻译：检测肉毒杆菌神经毒素的方法和组成
公开(公告)号：US20150253326A1
公开(公告)日：2015-09-10
申请号：US14624775
申请日：2015-02-18
申请人： Wisconsin Alumni Research Foundation
发明人： Edwin R. Chapman , Min Dong
IPC分类号： G01N33/569 , C07K14/435 , C07K14/705 , G01N33/542
CPC分类号： G01N33/573 , C07K14/435 , C07K14/43595 , C07K14/705 , C07K2319/50 , C07K2319/60 , C12Y304/24069 , G01N33/542 , G01N33/56911 , G01N2333/33 , G01N2333/952
摘要： The present invention provides a molecular construct capable of fluorescent resonance energy transfer (FRET), comprising a linker peptide, and donor and acceptor fluorophore moieties, where the linker peptide is a substrate of a botulinum neurotoxin selected from the group consisting of synaptobrevin, syntaxin and SNAP-25, or a fragment thereof capable being cleaved by the botulinum neurotoxin, and separates the donor and acceptor fluorophores by a distance of not more than 10 nm, and where emission spectrum of the donor fluorophore moiety overlaps with the excitation spectrum of the acceptor fluorophore moiety; or where the emission spectra of the fluorophores are detectably different. Also provided are isolated nucleic acid expressing the construct, kits comprising said construct and cell lines comprising said nucleic acid. Further provided are methods of detecting a BoNT using the above described construct via FRET, and methods for detecting a BoNT using surface plasmon resonance imaging.
摘要翻译：本发明提供了能够进行荧光共振能量转移（FRET）的分子构建体，其包含接头肽和供体和受体荧光团部分，其中连接肽是肉毒神经毒素的底物，其选自突触链突起目，突触蛋白和突触体 SNAP-25或其能够被肉毒杆菌神经毒素切割的片段，并且将供体和受体荧光团分开不超过10nm的距离，并且其中供体荧光团部分的发射光谱与受体的激发光谱重叠荧光团部分; 或者其中荧光团的发射光谱可检测地不同。还提供了表达构建体的分离的核酸，包含所述构建体的试剂盒和包含所述核酸的细胞系。还提供了通过FRET使用上述构造检测BoNT的方法，以及使用表面等离子体共振成像检测BoNT的方法。

9. 发明授权

US08969016B2 Resonance energy transfer assay with cleavage sequence and spacer 有权
标题翻译：具有切割序列和间隔区的共振能量转移测定
公开(公告)号：US08969016B2
公开(公告)日：2015-03-03
申请号：US13625723
申请日：2012-09-24
申请人： Biosentinel, Inc.
发明人： Robert D. Fish , Min Dong
IPC分类号： G01N33/53 , G01N31/00 , C07K19/00 , C12Q1/37 , G01N33/542 , G01N33/94
CPC分类号： C12Q1/37 , C07K14/00 , C07K14/47 , C07K19/00 , C07K2319/50 , C07K2319/60 , G01N21/6428 , G01N33/542 , G01N33/9406 , G01N2021/6432 , G01N2333/952
摘要： A molecular construct comprises a donor label, an acceptor label, a linker peptide disposed between the donor and the acceptor, the linker having a cleavage site sequence, and a spacer between at least one of (a) the donor and the cleavage site sequence and (b) the acceptor and the cleavage site sequence. Preferably, the construct is selected from the group consisting of CFP-(SGLRSRA)-SNAP-25-(SNS)-YFP, and CFP-(SGLRSRA)-synaptobrevin-(SNS)-YFP. In preferred embodiments, the linker peptide is a substrate of a botulinum neurotoxin selected from the group consisting of synaptobrevin (VAMP), syntaxin and SNAP-25, or a fragment thereof that can be recognized and cleaved by the botulinum neurotoxin. Advantageously, the spacer increases the electronic coupling between the donor label and the acceptor label relative to a corresponding construct without the spacer.
摘要翻译：分子构建体包括供体标记，受体标记，置于供体和受体之间的接头肽，接头具有切割位点序列，以及（a）供体和切割位点序列之间的间隔基和（b）受体和切割位点序列。优选地，构建体选自CFP-（SGLRSRA）-SNAP-25-（SNS）-YFP和CFP-（SGLRSRA）-Synaptobrevin-（SNS）-YFP。在优选的实施方案中，接头肽是肉毒杆菌神经毒素的底物，其选自由肉毒杆菌神经毒素可识别和切割的突触连锁蛋白（VAP），突触蛋白（S）或SNAP-25，或其片段。有利地，间隔物相对于没有间隔物的相应构建体增加了供体标签和受体标记之间的电子偶联。

10. 发明申请

US20150045286A1 BROAD SPECTRUM ANTIBIOTIC ARYLOMYCIN ANALOGS 审中-公开
标题翻译： BROAD SPECTRUM抗生素ARYLOMYCIN模拟
公开(公告)号：US20150045286A1
公开(公告)日：2015-02-12
申请号：US14384638
申请日：2013-03-08
申请人： The Scripps Research Institute
发明人： Floyd E. Romesberg , Peter A. Smith , Tucker C. Roberts
IPC分类号： C07K7/56
CPC分类号： C07K7/06 , A61K9/0014 , A61K38/00 , A61K38/08 , A61K45/06 , C07K7/56 , C12N9/52 , C12Q1/04 , C12Q1/18 , C12Q1/37 , C12Q1/689 , C12Q2600/156 , G01N33/56911 , G01N33/56916 , G01N33/56927 , G01N33/56938 , G01N2333/952 , G01N2469/10 , Y02A50/47 , Y02A50/471 , Y02A50/473 , Y02A50/475 , Y02A50/481
摘要： Arylomycin analogs are provided, wherein the analogs can have broad spectrum bioactivity. Resistance to the antibiotic bioactivity of natural product arylomycin in a range of pathogenic bacterial species has been found to depend upon single amino acid mutations at defined positions of bacterial Signal Peptidases (SPases), wherein the presence of a proline residue confers arylomycin resistance. Arylomycin analogs are provided herein that can overcome that resistance and provide for a broader spectrum of antibiotic bioactivity than can natural product arylomycins such as arylomycin A2. Methods for determining if a bacterial strain is susceptible to narrow spectrum arylomycin antibiotics, or if a broad spectrum analog is required for treatment, is provided. Pharmaceutical compositions and methods of treatment of bacterial infections, and methods of synthesis of arylomycin analogs, are provided.
摘要翻译：提供了芳香霉素类似物，其中类似物可具有广谱生物活性。已经发现天然产物芳香霉素在一系列病原菌种中对抗生素生物活性的抗性取决于在细菌信号肽酶（SPases）的限定位置的单个氨基酸突变，其中脯氨酸残基的存在赋予芳樟霉素抗性。本文提供的芳香霉素类似物可以克服该抗性，并提供比天然产物芳香霉素如芳香霉素A2更广谱的抗生素生物活性。确定细菌菌株是否易受窄谱芳香霉素抗生素敏感的方法，或者如果需要广谱类似物进行治疗。提供了治疗细菌感染的药物组合物和方法，以及合成法罗霉素类似物的方法。

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