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    • 3. 发明申请
    • NOVEL GLYCOSYLTRANSFERASE GENE AND USE THEREOF
    • 新型GLYCOSYLTRANSFERASE基因及其用途
    • US20170058269A1
    • 2017-03-02
    • US15308117
    • 2015-05-01
    • SUNTORY HOLDINGS LIMITED
    • Yoshikazu TANAKANaoko OKITSUKeisuke MATSUI
    • C12N9/10C12P17/06
    • C12N9/1048A01H5/00C12N5/10C12N9/10C12N15/09C12P17/06C12P19/60C12P21/00C12Y204/00
    • Provided is a polynucleotide for encoding a protein that exhibits activity for transferring a sugar to a hydroxyl group at position 7 of a flavone, particularly flavone 4′-glucoside. A polynucleotide selected from the group consisting of: (a) polynucleotides comprising a base sequence of SEQ ID NO.: 1 or SEQ ID NO.: 5; (b) polynucleotides that hybridize, under stringent conditions, with polynucleotides comprising a base sequence complementary to a base sequence of SEQ ID NO.: 1 or SEQ ID NO.: 5, wherein the polynucleotides encode a protein that exhibits activity for transferring a sugar to a hydroxyl group at position 7 of a flavone; (c) polynucleotides for encoding proteins comprising an amino acid sequence of SEQ ID NO.: 2 or SEQ ID NO.: 6; (d) polynucleotides for encoding proteins comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted, and/or added in an amino acid sequence of SEQ ID NO.: 2 or SEQ ID NO.: 6, the polynucleotides exhibiting activity for transferring a sugar to a hydroxyl group at position 7 of a flavone; and the like.
    • 本发明提供一种编码蛋白质的多核苷酸,其表现出将糖转移到黄酮,特别是黄酮4'-葡萄糖苷的第7位的羟基的活性。 选自以下的多核苷酸:(a)包含SEQ ID NO:1或SEQ ID NO:5的碱基序列的多核苷酸; (b)在严格条件下与包含与SEQ ID NO:1或SEQ ID NO:5的碱基序列互补的碱基序列的多核苷酸杂交的多核苷酸,其中所述多核苷酸编码显示转移糖的活性的蛋白质 到黄酮的第7位的羟基; (c)编码包含SEQ ID NO:2或SEQ ID NO:6的氨基酸序列的蛋白质的多核苷酸; (d)用于编码蛋白质的多核苷酸,其包含其中一个或多个氨基酸已被缺失,取代,插入和/或加入到SEQ ID NO:2或SEQ ID NO:6的氨基酸序列中的氨基酸序列 表现出将糖转移到黄酮第7位羟基的活性的多核苷酸; 等等。