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1. 发明授权

US08043851B2 DNA encoding a neoculin acidic subunit of curculin 有权
标题翻译：编码仙人掌的新霉素酸性亚基的DNA
公开(公告)号：US08043851B2
公开(公告)日：2011-10-25
申请号：US12896676
申请日：2010-10-01
申请人： Keiko Abe , Tomiko Asakura , Hiroyuki Sorimachi , Tazuko Uenoyama , Kenichiro Nakajima , Katsuhiko Kitamoto , Junichi Maruyama , Mikiya Kishi
发明人： Keiko Abe , Tomiko Asakura , Hiroyuki Sorimachi , Tazuko Uenoyama , Kenichiro Nakajima , Katsuhiko Kitamoto , Junichi Maruyama , Mikiya Kishi
IPC分类号： C12N15/00 , C07H21/02
CPC分类号： C07K14/47 , A23L27/31
摘要： Disclosed is a polypeptide shown in the following in (A), and a protein dimer neoculin comprising the polypeptide Neoculin Acidic Subunit (NAS) and the polypeptide Neoculin Basic Subunit (NBS) and having a taste-modifying activity: (A) a polypeptide comprising an amino acid sequence shown in SEQ ID NO.2 in the sequence listing.
摘要翻译：公开了以下（A）中所示的多肽和包含多肽新霉素酸性亚基（NAS）和多肽新霉素基础亚基（NBS）并具有调味活性的蛋白质二聚体新霉素：（A）包含序列表中SEQ ID NO：2所示的氨基酸序列。

2. 发明授权

US07446192B2 Gene participating in acetic acid tolerance, acetic acid bacteria bred using the gene, and process for producing vinegar with the use of the acetic acid bacteria 有权
标题翻译：基因参与乙酸耐量，使用该基因繁殖的乙酸菌，以及使用乙酸菌生产醋的方法
公开(公告)号：US07446192B2
公开(公告)日：2008-11-04
申请号：US10507446
申请日：2003-03-12
申请人： Hidetsugu Goto , Shigeru Nakano
发明人： Hidetsugu Goto , Shigeru Nakano
IPC分类号： C07H21/04
CPC分类号： C12J1/04 , C07K14/195 , C12N1/20
摘要： The present invention is intended to provide novel genes participating in acetic acid tolerance of acetic acid bacteria, and a method of improving acetic acid tolerance of microorganisms, particularly that of acetic acid bacteria by using the genes, further a method of efficiently producing vinegar with acetic acid at higher concentration by using acetic acid bacteria whose acetic acid tolerance is improved. In the present invention, novel genes having a function for improving acetic acid tolerance on practical level were cloned from practical acetic acid bacteria belonging to the genus Gluconacetobacter by a method of obtaining genes from chromosomal DNA library that enable to grow on the medium at a high concentration of acetic acid. Further, in transformants in which the genes were introduced into acetic acid bacteria, acetic acid tolerance was remarkably increased, and when the transformants are subjected to aeration culture in the presence of ethanol, the growth lag-time can be shortened, and the growth rate can also be improved, moreover the final acetic acid concentration can be remarkably improved.
摘要翻译：本发明旨在提供参与乙酸菌的乙酸耐受性的新基因，以及通过使用该基因来提高微生物，特别是乙酸菌的乙酸耐受性的方法，还提出了一种有效生产乙酸醋的方法通过使用醋酸耐受性提高的乙酸菌，浓度较高的酸。在本发明中，通过从染色体DNA文库获得能够在培养基上高生长的基因的方法，从属于葡糖型醋杆菌属的实用乙酸菌克隆了具有实用水平提高乙酸耐受性的新型基因乙酸浓度。此外，在将基因导入乙酸菌的转化体中，乙酸耐受性显着增加，当转化体在乙醇存在下进行通气培养时，可以缩短生长滞后时间，生长速度也可以改善，而且可以显着提高最终的乙酸浓度。

3. 发明授权

US07354751B2 Alcohol dehydrogenase gene of acetic acid bacterium 有权
标题翻译：乙酸细菌的酒精脱氢酶基因
公开(公告)号：US07354751B2
公开(公告)日：2008-04-08
申请号：US10548486
申请日：2004-02-17
申请人： Shigeru Nakano
发明人： Shigeru Nakano
IPC分类号： C07H21/04 , C12P21/06 , C12N9/02 , C12N1/21
CPC分类号： C12N9/0006 , C12J1/04 , C12P7/54
摘要： A novel gene having a function of improving acetic acid fermentation in practical level was cloned from a practical acetic acid bacterium belonging to the genus Gluconacetobacter by a method for obtaining a gene having a growth-promoting function in acetic acid-containing medium from the chromosomal DNA library of the acetic acid bacterium. It was made possible to significantly shorten the growth induction period and significantly improve the acetic acid fermentation rate of transformants obtained by introducing the gene into acetic acid bacteria, when such transformants are cultured in the presence of ethanol.
摘要翻译：通过从染色体DNA获得含有乙酸的培养基中具有生长促进功能的基因的方法，从属于葡糖型醋杆菌属的实用乙酸菌克隆了具有实用水平的乙酸发酵功能的新型基因醋酸菌库。通过在乙醇存在下培养这些转化体，可以显着缩短生长诱导期，显着提高通过将基因导入乙酸菌而得到的转化体的乙酸发酵率。

4. 发明申请

US20060286652A1 Gene improving temperature-tolerance of acetic acid bacterium, acetic acid bacterium bred using the gene and process for producing vinegar using the acetic acid bacterium 有权
标题翻译：基因改良乙酸菌的耐温性，使用该基因繁殖的乙酸菌和使用醋酸菌生产醋的方法
公开(公告)号：US20060286652A1
公开(公告)日：2006-12-21
申请号：US10538481
申请日：2003-12-04
申请人： Hidetsugu Goto
发明人： Hidetsugu Goto
IPC分类号： C12P7/54 , C12N9/02 , C12N15/74 , C12N1/21
CPC分类号： C12N9/1051 , C12J1/04 , C12P7/54
摘要： The present invention is intended to provide a novel gene originating in an acetic acid bacterium, which participates in temperature tolerance; a method of improving the temperature tolerance of a microorganism, in particular, an acetic acid bacterium using the above gene; and a method of efficiently producing vinegar having a higher acetic acid concentration with the use of an acetic acid bacterium whose temperature tolerance has been improved.
摘要翻译：本发明旨在提供一种参与温度耐受性的乙酸菌中源自于基因的新基因; 提高微生物，特别是使用上述基因的乙酸菌的耐温性的方法; 以及使用耐温性提高的乙酸菌有效生产乙酸浓度较高的醋的方法。

5. 发明申请

US20060154351A1 Gene participating in acetic acid tolerance, acetic acid bacteria bred using the gene, and process for producing vinegar with the use of the acetic acid bacterium 有权
标题翻译：基因参与乙酸耐量，使用该基因繁殖的乙酸菌，以及使用乙酸菌生产醋的方法
公开(公告)号：US20060154351A1
公开(公告)日：2006-07-13
申请号：US10507446
申请日：2003-03-12
申请人： Hidetsugu Goto , Shigeru Nakano
发明人： Hidetsugu Goto , Shigeru Nakano
IPC分类号： C12P7/54 , C12N9/02 , C12N15/74 , C12N1/21
CPC分类号： C12J1/04 , C07K14/195 , C12N1/20
摘要： The present invention is intended to provide novel genes participating in acetic acid tolerance of acetic acid bacteria, and a method of improving acetic acid tolerance of microorganisms, particularly that of acetic acid bacteria by using the genes, further a method of efficiently producing vinegar with acetic acid at higher concentration by using acetic acid bacteria whose acetic acid tolerance is improved. In the present invention, novel genes having a function for improving acetic acid tolerance on practical level were cloned from practical acetic acid bacteria belonging to the genus Gluconacetobacter by a method of obtaining genes from chromosomal DNA library that enable to grow on the medium at a high concentration of acetic acid. Further, in transformants in which the genes were introduced into acetic acid bacteria, acetic acid tolerance was remarkably increased, and when the transformants are subjected to aeration culture in the presence of ethanol, the growth lag-time can be shortened, and the growth rate can also be improved, moreover the final acetic acid concentration can be remarkably improved.
摘要翻译：本发明旨在提供参与乙酸菌的乙酸耐受性的新基因，以及通过使用该基因来提高微生物，特别是乙酸菌的乙酸耐受性的方法，还提出了一种有效生产乙酸醋的方法通过使用醋酸耐受性提高的乙酸菌，浓度较高的酸。在本发明中，通过从染色体DNA文库获得能够在培养基上高生长的基因的方法，从属于葡糖型醋杆菌属的实用乙酸菌克隆了具有实用水平提高乙酸耐受性的新型基因乙酸浓度。此外，在将基因导入乙酸菌的转化体中，乙酸耐受性显着增加，当转化体在乙醇存在下进行通气培养时，可以缩短生长滞后时间，生长速度也可以改善，而且可以显着提高最终的乙酸浓度。

6. 发明授权

US07541491B2 Gene involved in growth-promoting function of acetic acid bacteria and uses thereof 有权
标题翻译：涉及乙酸菌生长促进功能的基因及其用途
公开(公告)号：US07541491B2
公开(公告)日：2009-06-02
申请号：US10561834
申请日：2004-06-16
申请人： Shigeru Nakano
发明人： Shigeru Nakano
IPC分类号： C07C51/16 , C12N15/00 , C12N1/20 , C07H21/02
CPC分类号： C07K14/195 , C12J1/00 , C12J1/04
摘要： The present invention provides a gene involved in the growth-promoting function of acetic acid bacteria and a microorganism containing the gene. In particular, the present invention provides a method for enhancing the growth-promoting function of acetic acid bacteria and a method for efficiently producing vinegar containing acetic acid at a high concentration in a short time using such acetic acid bacteria having an enhanced growth-promoting function.
摘要翻译：本发明提供了涉及乙酸菌生长促进功能的基因和含有该基因的微生物。特别地，本发明提供了一种提高乙酸菌生长促进功能的方法以及使用具有增强生长促进功能的乙酸菌在短时间内高效浓缩高浓度乙酸醋的方法。

7. 发明授权

US07256025B2 Gene improving temperature-tolerance of acetic acid bacterium, acetic acid bacterium bred using the gene and process for producing vinegar using the acetic acid bacterium 有权
标题翻译：基因改良乙酸菌的耐温性，使用该基因繁殖的乙酸菌和使用乙酸菌生产醋的方法
公开(公告)号：US07256025B2
公开(公告)日：2007-08-14
申请号：US10538481
申请日：2003-12-04
申请人： Hidetsugu Goto
发明人： Hidetsugu Goto
IPC分类号： C12N9/10 , C12N1/20 , C12N15/74 , C12P7/54 , C12P21/06
CPC分类号： C12N9/1051 , C12J1/04 , C12P7/54
摘要： The present invention is intended to provide a novel gene originating in an acetic acid bacterium, which participates in temperature tolerance; a method of improving the temperature tolerance of a microorganism, in particular, an acetic acid bacterium using the above gene; and a method of efficiently producing vinegar having a higher acetic acid concentration with the use of an acetic acid bacterium whose temperature tolerance has been improved.
摘要翻译：本发明旨在提供一种参与温度耐受性的乙酸菌中源自于基因的新基因; 提高微生物，特别是使用上述基因的乙酸菌的耐温性的方法; 以及使用耐温性提高的乙酸菌有效生产乙酸浓度较高的醋的方法。

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