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1. 发明授权

US07138504B2 Reagents and methods for mycophenolic acid immunoassay 有权
公开(公告)号：US07138504B2
公开(公告)日：2006-11-21
申请号：US11201992
申请日：2005-08-11
申请人： Vani B. Bodepudi , Amy P. Tsai , Weixing Luo , Rueyming Loor
发明人： Vani B. Bodepudi , Amy P. Tsai , Weixing Luo , Rueyming Loor
IPC分类号： C07K17/06 , C07K16/44 , C12N9/96 , G01N33/353 , G01N33/533
CPC分类号： G01N33/9493 , C07K16/44
摘要： The invention teaches derivatives of mycophenolic alcohol and methods of preparing immunogens and other conjugates useful in immunoassays for quantitatively measuring concentrations of mycophenolic acid (MPA) and/or active metabolites of MPA in patient specimens. Antibodies produced from the disclosed immunogens capable of binding to MPA with cross-reactivity of no more than 5% with inactive metabolites and commonly co-prescribed drugs. Further, immunoassays for measuring the concentration of MPA using such antibodies are taught.

2. 发明授权

US06946547B2 Ecstasy-class analogs and use of same in detection of ecstasy-class compounds 有权
标题翻译：摇头丸类类似物，并用于检测狂喜类化合物
公开(公告)号：US06946547B2
公开(公告)日：2005-09-20
申请号：US10457314
申请日：2003-06-09
申请人： Riaz Rouhani , Anthony de Jesus Sanchez , David Davoudzadeh , William A. Coty , Cynthia A. Vistica
发明人： Riaz Rouhani , Anthony de Jesus Sanchez , David Davoudzadeh , William A. Coty , Cynthia A. Vistica
IPC分类号： A61K47/48 , A61K49/16 , C07D317/58 , C07K16/44 , G01N33/94 , G01N33/533 , G01N33/535
CPC分类号： G01N33/946 , A61K47/643 , A61K47/646 , A61K49/16 , C07D317/58 , C07K16/44
摘要： The present invention provides a system for the improved detection of ecstasy-class compounds in biological samples. New ecstasy-class analogs are provided for detection of such ecstasy-class drugs. These analogs are compounds, or salts thereof, of a 2-amino-methylenedioxyphenyl (MDP) derivative attached to Z, where Z is a moiety capable of bonding, either directly or indirectly, with an immunogenic carrier, a detectable label, or a solid capture vehicle. Such analogs may be used to construct immunogens, enzyme or enzyme-donor conjugates, and other conjugates. The immunogens reproducibly generate antibodies with an exquisite ability to distinguish various ecstasy-class drugs in biological samples from potentially interfering substances. The specific antibodies and the conjugates may be used to distinguish and measure various ecstasy-class compounds in biological samples, such as those obtained from an individual suspected of substance abuse. In another aspect, the invention includes certain reagents, reagent combinations, and kits for performing assay methods for ecstasy-class compounds in a biological sample.
摘要翻译：本发明提供一种用于改善生物样品中摇头丸类化合物检测的系统。提供新的摇头丸类似物用于检测这类摇头丸类药物。这些类似物是与Z连接的2-氨基亚甲二氧基苯基（MDP）衍生物的化合物或其盐，其中Z是能够直接或间接与免疫原性载体，可检测标记或固体键合的部分捕捉车辆此类类似物可用于构建免疫原，酶或酶 - 供体缀合物和其它缀合物。免疫原可重复产生具有精确的能力的抗体，以区分生物样品中的各种令人惊喜的药物与潜在的干扰物质。特异性抗体和缀合物可以用于区分和测量生物样品中的各种摇头丸类化合物，例如从怀疑有药物滥用的个体获得的那些化合物。另一方面，本发明包括某些试剂，试剂组合和用于在生物样品中进行摇头丸类化合物的测定方法的试剂盒。

3. 发明授权

US5223393A Detection of analytes having binding sites for at least two binding moieties 失效
标题翻译：检测具有两个绑定机构的绑定站点的分析人员
公开(公告)号：US5223393A
公开(公告)日：1993-06-29
申请号：US537905
申请日：1990-06-12
申请人： Pyare Khanna , Reuyming Loor
发明人： Pyare Khanna , Reuyming Loor
IPC分类号： C12Q1/34 , C12Q1/68 , G01N33/535 , G01N33/536 , G01N33/542 , G01N33/58
CPC分类号： G01N33/542 , C12Q1/6818 , G01N33/535 , G01N33/581
摘要： The present invention provides reagents for a detectable label for use in specific binding assays. Specifically, modified .beta.-galactosidase enzyme donors (ED) and acceptors (EA) are utilized. Both ED and EA are modified to form separate ED and EA complexes by coupling each with a linking element and a binding moiety which is specific to a binding site in the analyte. The ED and EA complexes are incapable of forming active enzyme in the absence of the analyte. However, when analyte is present, ED and EA complexes which bind to a common analyte in a sample, active .beta.-galactosidase is formed.

4. 发明授权

US5362625A Methods and compositions for enzyme complementation assays using the omega region of .beta.-galactosidase 失效
标题翻译：使用β-半乳糖苷酶的ω区域进行酶互补测定的方法和组合物
公开(公告)号：US5362625A
公开(公告)日：1994-11-08
申请号：US67342
申请日：1993-05-25
申请人： Mark Krevolin , David Kates
发明人： Mark Krevolin , David Kates
IPC分类号： C07K14/195 , C07K14/00 , C07K14/41 , C07K19/00 , C12N9/38 , C12N15/00 , C12N15/09 , C12Q1/34 , G01N33/53 , G01N33/58 , G01N33/535
CPC分类号： G01N33/581
摘要： The use of omega-acceptor and omega-donor polypeptides (comprising about two-thirds and one-third of the .beta.-galactosidase molecule amino and carboxyl termini, respectively), prepared by recombinant DNA techniques, DNA synthesis, or chemical polypeptide synthesis techniques, which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of .beta.-galactosidase, is described along with improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample.
摘要翻译：通过重组DNA技术，DNA合成或化学多肽合成技术制备的ω-受体和ω-供体多肽（分别包含约三分之二和三分之一的β-半乳糖苷酶分子氨基和羧基末端）其能够相互作用以形成具有β-半乳糖苷酶特征的催化活性的活性酶复合物，以及改进的方法和用于酶互补测定的新组合物用于定性和定量测定样品中的可疑分析物。

5. 发明授权

US5244786A Method of measuring available free thyroxine bending sites 失效
标题翻译：测量可用的游离甲状腺素弯曲部位的方法
公开(公告)号：US5244786A
公开(公告)日：1993-09-14
申请号：US568644
申请日：1990-08-13
申请人： Teresa K. Picone , Stephen B. Friedman
发明人： Teresa K. Picone , Stephen B. Friedman
IPC分类号： G01N33/58 , G01N33/78
CPC分类号： G01N33/581 , G01N33/78
摘要： The present invention provides an improved assay for measuring thyroxine uptake by thyroxine binding globulin in which thyroxine binding globulin (TBG) activity is measured directly. The method comprises combining a sample in an aqueous solution with an enzyme donor (ED) conjugated to an analogue of polyiodothyronine that competes with thyroxine for thyroxine binding globulin binding sites. An enzyme acceptor (EA) characterized by providing a modulated enzyme activity in relation to the amount of TBG activity is combined with an enzyme donor and sample in an aqueous solution. The amount of enzyme activity in comparison to a control solution having a known amount of available TBG binding sites is determined.
摘要翻译：本发明提供了用于测量甲状腺素结合球蛋白甲状腺素摄取的改进测定法，其中甲状腺素结合球蛋白（TBG）活性被直接测量。该方法包括将水溶液中的样品与与甲状腺素竞争甲状腺素结合球蛋白结合位点的与多聚甲状腺氨酸类似物缀合的酶供体（ED）组合。特征在于提供与TBG活性的量相关的调节的酶活性的酶受体（EA）与酶供体和样品在水溶液中组合。确定与具有已知量的可用TBG结合位点的对照溶液相比，酶活性的量。

6. 发明授权

US5120653A Vector comprising DNA sequence coding for enzyme-donor polypeptide 失效
标题翻译：包含编码酶供体多肽的DNA序列的载体
公开(公告)号：US5120653A
公开(公告)日：1992-06-09
申请号：US788370
申请日：1985-10-22
申请人： Daniel R. Henderson
发明人： Daniel R. Henderson
IPC分类号： C07K14/02 , C07K14/59 , C12N1/21 , C12N9/38 , C12N15/62 , G01N33/535
CPC分类号： C07K14/005 , C07K14/59 , C12N15/62 , C12N9/2471 , C12Y302/01023 , G01N33/535 , C07K2319/00 , C07K2319/02 , C07K2319/35 , C07K2319/40 , C07K2319/61 , C07K2319/75 , C12N2730/10122
摘要： This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques, DNA synthesis or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of .beta.-galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.
摘要翻译：本发明涉及用于定量和定量测定样品中可疑分析物的酶互补测定法的改进方法和新型组合物。描述了通过重组DNA技术制备的酶 - 受体和酶供体多肽的使用，能够相互作用以形成具有β-半乳糖苷酶特征的催化活性的活性酶复合物的DNA合成或化学多肽合成技术。描述了利用这些多肽的均相和异质测定。

7. 发明授权

US4956274A Reagent stabilization in enzyme-donor and acceptor assay 失效
标题翻译：酶供体和受体测定中的试剂稳定性
公开(公告)号：US4956274A
公开(公告)日：1990-09-11
申请号：US34757
申请日：1987-04-06
申请人： Pyare L. Khanna , Robert Dworschack , Phillip Ruprecht
发明人： Pyare L. Khanna , Robert Dworschack , Phillip Ruprecht
IPC分类号： C12N9/40 , C07K1/00 , C12N9/96 , C12Q1/34 , G01N33/535 , G01N33/58
CPC分类号： G01N33/581 , C12Q1/34 , G01N2333/924 , Y10S435/81 , Y10S435/963
摘要： A method of stabilizing .beta.-galactosidase peptide fragments against storage degradation for use in a complementation assay which comprises storing the peptide fragment in a storage medium containing an ionic surfactant or a surfactant derived from a sugar residue and adding a cyclodextrin to the storage medium after storing the peptide fragments but before using them in a complementation assay.

8. 发明授权

US11578140B2 Immunoassay for mitragynine 有权
公开(公告)号：US11578140B2
公开(公告)日：2023-02-14
申请号：US16662408
申请日：2019-10-24
申请人： MICROGENICS CORPORATION
发明人： Guoping Xu , Imad Nashashibi , Pong Chua , Chandrasekaran Raman , Yingqi Lin , Lakshmi Anne , Anthony Prestigiacomo
IPC分类号： C07D471/14 , G01N33/94 , C07K16/44 , G01N33/53
摘要： Compositions, methods, assays, and kits providing or incorporating derivatives of mitragynine, particularly as haptens and immunogens.

9. 发明授权

US5188938A Enzyme quantitation wicking assay 失效
标题翻译：酶定量芯吸试验
公开(公告)号：US5188938A
公开(公告)日：1993-02-23
申请号：US777489
申请日：1991-10-15
申请人： Pyare L. Khanna , Glenda L. Choate
发明人： Pyare L. Khanna , Glenda L. Choate
IPC分类号： G01N33/542 , G01N33/558 , G01N33/58
CPC分类号： G01N33/581 , G01N33/542 , G01N33/558 , Y10S435/97 , Y10S435/975
摘要： Diagnostic assays are provided comprising complementary enzyme fragments of .beta.-galactosidase, where one of the fragments is bound to a support and the other fragment is conjugated to an immunologically cross-reactive epitope of the analyte or complementary to an analyte receptor. Binding to the receptor allows for discrimination between complexed enzyme fragment conjugate and uncomplexed enzyme fragment conjugate. The assay medium is then allowed to wick onto a support to which the complementary fragment is substantially uniformly bound in the detection region. The support is then developed, where color formation from the substrate in the detection region is used as a measure of the presence of analyte in the sample.
摘要翻译：提供诊断测定法，其包括β-半乳糖苷酶的互补酶片段，其中一个片段与载体结合，另一个片段与分析物的免疫交叉反应性表位缀合或与分析物受体互补。与受体的结合允许区分复合酶片段缀合物和未复合酶片段缀合物。然后允许测定培养基吸收到在检测区域中互补片段基本上均匀结合的载体上。然后开发支持体，其中使用检测区域中底物的颜色形成作为样品中分析物存在的量度。

10. 发明授权

US5037735A Visual discrimination qualitative enzyme complementation assay 失效
标题翻译：视觉鉴别定性酶互补测定
公开(公告)号：US5037735A
公开(公告)日：1991-08-06
申请号：US210825
申请日：1988-06-24
申请人： Pyare L. Khanna , Glenda L. Choate
发明人： Pyare L. Khanna , Glenda L. Choate
IPC分类号： G01N33/543 , G01N33/542 , G01N33/558 , G01N33/58
CPC分类号： G01N33/581 , G01N33/558
摘要： A .beta.-galactosidase complementation assay for determining the presence of an analyte which is a member of a specific binding pair (sbp) in a sample is provided, which assay permits visual discrimination between those samples wherein the analyte is present above a predetermined, threshold concentration. The method comprises combining the sample with a hydrophobic enzyme donor- (ED-) analyte conjugate and the complementary member of the sbp to form an sbp complex-containing assay medium. A small volume of the assay medium is spotted onto an enzyme acceptor (EA) affixed to a bibulous, solid support, followed by development with enzyme substrate solution. A substantially larger area is detectable when analyte in the sample is below as compared to above a threshold concentration. The assay is particularly useful in field testing applications such as determining the presence of antibiotics in milk, toxins in water, or drugs in serum or urine. Kits facilitating the method are also provided.

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