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1. 发明申请

US20110217727A1 METHOD FOR PATTERNING MAGNETIC MATERIALS IN LIVE CELL, METHOD FOR IMAGING PATTERN OF MAGNETIC MATERIALS, AND APPARATUS USED FOR SAME 审中-公开
标题翻译：用于在活细胞中形成磁性材料的方法，用于成像磁性材料的图案的方法以及用于其的装置
公开(公告)号：US20110217727A1
公开(公告)日：2011-09-08
申请号：US13128376
申请日：2009-11-09
申请人： Dae Joong Kim
发明人： Dae Joong Kim
IPC分类号： C12Q1/02 , C12M1/34 , B82Y15/00
CPC分类号： C12N13/00
摘要： The present invention provides a method for imaging a pattern of magnetic materials in a live cell comprising: preparing a plurality of magnetic materials magnetized in a direction of a line of magnetic force by applying a magnetic field, at least one of the magnetic materials being configured into a nanoparticle and the surface of at least one of the magnetic materials being modified; introducing a plurality of the magnetic materials into each live cell; providing the live cell with a label capable of binding to the magnetic material and imaging a pattern of the magnetic materials in a direction of a line of magnetic force; allowing a bundle of the lines of magnetic force to pass through the live cell in a direction by applying a focused magnetic field to the live cell; aligning a plurality of the magnetic materials in the live cell with the direction of the line of magnetic force by the applied magnetic field; and identifying an imaged pattern of the label capable of imaging the aligned pattern of the magnetic materials.
摘要翻译：本发明提供了一种用于对活细胞中的磁性材料图案进行成像的方法，包括：通过施加磁场来制备在磁力线的方向上磁化的多个磁性材料，所述磁性材料中的至少一个被配置进入纳米颗粒，并且至少一种磁性材料的表面被改性; 将多个磁性材料引入每个活细胞; 为活细胞提供能够结合磁性材料的标签，并且在磁力线的方向上成像磁性材料的图案; 允许一束磁力线通过向活细胞施加聚焦磁场而沿着方向通过活细胞; 通过所施加的磁场将活细胞中的多个磁性材料与磁力线的方向对准; 以及识别能够成像磁性材料的对准图案的标签的成像图案。

2. 发明申请

US20110201016A1 METHOD FOR DETECTING MODULATOR FOR REGULATING ACTIVITY OF CELLULAR COMPONENT IN CELL 审中-公开
标题翻译：检测用于调节细胞中细胞成分活性的调节剂的方法
公开(公告)号：US20110201016A1
公开(公告)日：2011-08-18
申请号：US13025352
申请日：2011-02-11
申请人： Dae Joong KIM
发明人： Dae Joong KIM
IPC分类号： G01N33/53
CPC分类号： G01N33/502 , G01N33/54326 , G01N33/582
摘要： A method for detecting a modulator that can regulate biological and/or physiological activity of a cellular component is provided. The method can detect a modulator, after determining whether there is a competitive reaction between a reference substance and a modulator toward the cellular component in a cell by imaging a magnetic pattern magnetized in a direction of a magnetic line of force and a pattern of a label bound to the cellular component. The method has a benefit that since the reaction of a cellular component with a reference substance and a modulator such as a drug occurs in a cell, the competition reaction between the reference substance and the modulator toward the cellular component can reflect the real metabolism occurred in a live cell. It can also allow the result of interaction between the cellular component and the modulator such as a drug to be visualized and to be identified directly in a live cell. Additionally, the method has advantages to screen many modulators using a few reference substances and to detect the functions of the modulator in a cell, e.g., its efficacy without modifying the modulator by introducing the reference substance.
摘要翻译：提供了一种用于检测可调节细胞成分的生物学和/或生理活性的调节剂的方法。该方法可以通过对在磁力线的方向上磁化的磁性图案和标签的图案进行成像来确定在参考物质和调节剂之间是否存在对细胞中的细胞成分的竞争反应的方法来检测调节剂结合细胞成分。该方法的优点在于，由于细胞成分与参考物质和调节剂如药物的反应发生在细胞中，所以参考物质与调节剂之间的竞争反应朝向细胞成分可以反映出在一个活细胞它还可以允许细胞组分和调节剂如药物之间的相互作用的结果被可视化并且直接在活细胞中鉴定。此外，该方法具有使用少量参考物质筛选许多调节剂并且检测细胞中调节剂的功能的优点，例如其功效而不通过引入参考物质来修饰调节剂。

3. 发明申请

US20110223666A1 CYTOPLASM EXPOSURE ADDITIVE AND METHOD FOR EXPOSING PARTICLES DELIVERED INTO CELL TO CYTOPLASM FROM ENDOCYTIC VESICLES IN INTACT CELL 审中-公开
标题翻译： CYTOPLASM曝光添加剂和方法，用于将细胞递送至细胞内的细胞因子在内源性细胞中的内源性VESICLES
公开(公告)号：US20110223666A1
公开(公告)日：2011-09-15
申请号：US13128327
申请日：2009-11-09
申请人： Dae Joong Kim
发明人： Dae Joong Kim
IPC分类号： C12N5/071 , C07C39/15 , C07D207/448 , C07C47/04 , C07C43/303 , C07C31/04 , C07C31/08 , C01C1/16 , B82Y5/00
CPC分类号： C07D207/448 , C07C29/94 , C07C45/90 , C07C31/04 , C07C31/08 , C07C47/04
摘要： The present invention relates to a method of exposing particles to cytoplasm comprising introducing particles into a live cell; allowing the live cell to contact a cytoplasm exposure additive which can expose the particles from endocytic vesicles to cytoplasm in the cell with maintaining its physiological, biochemical, or biological environment as undamaged; and allowing the particles to be exposed from the endocytic vesicles to the cytoplasm. The present invention is advantageous in that particles delivered into cells can be effectively exposed to cytoplasm from endocytic vesicles in intact cells which maintain their physiological, biochemical, or biological environment as undamaged.
摘要翻译：本发明涉及将颗粒暴露于细胞质的方法，包括将颗粒引入活细胞; 允许活细胞接触细胞质暴露添加剂，其可以将颗粒从细胞内细胞囊泡暴露于细胞中的细胞质，同时保持其生理，生物化学或生物环境未损伤; 并允许颗粒从胞吞囊泡暴露于细胞质。本发明的优点在于，递送到细胞中的颗粒可以在完整的细胞中有效地暴露于来自内细胞囊泡的细胞质，其保持其未受损害的生理，生物化学或生物环境。

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