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1. 发明申请

US20120178174A1 FLUORESCENT PROBE 有权
标题翻译：荧光探针
公开(公告)号：US20120178174A1
公开(公告)日：2012-07-12
申请号：US13425802
申请日：2012-03-21
申请人： Tetsuo NAGANO , Yu GABE , Yasuteru URANO
发明人： Tetsuo NAGANO , Yu GABE , Yasuteru URANO
IPC分类号： G01N21/64 , C07F5/02
CPC分类号： C07F5/022 , C09K11/06 , C09K2211/1055 , G01N31/22 , Y10T436/145555 , Y10T436/177692
摘要： A compound represented by the formula (I) wherein R1 and R2 represent amino groups that substitute at adjacent positions on the benzene ring, wherein one of the amino groups may have one alkyl group; R3 and R4 represent hydrogen atom, a C1-6 alkyl group, or a C1-6 alkoxy group, R5 and R8 independently represent a C1-6 alkyl group, R6 and R9 represent a carboxy-substituted C1-6 alkyl group, an alkoxycarbonyl-substituted C1-6 alkyl group, a sulfo-substituted C1-6 alkyl group, or an alkyl sulfonate-substituted C1-6 alkyl group, and R7 and R10 represent a C1-6 alkyl group, an aryl group, a C1-6 alkoxycarbonyl group, a vinyl group, a thienyl group, or a pyrrolyl group, and an agent for measurement of nitrogen monoxide containing the compound. Said compound can give a fluorescent substance that is free from change in fluorescence intensity in a wide pH range.
摘要翻译：由式（I）表示的化合物，其中R 1和R 2表示在苯环上的相邻位置取代的氨基，其中一个氨基可以具有一个烷基; R3和R4表示氢原子，C1-6烷基或C1-6烷氧基，R5和R8独立地表示C1-6烷基，R6和R9表示羧基取代的C1-6烷基，烷氧基羰基取代的C 1-6烷基，磺基取代的C 1-6烷基或烷基磺酸酯取代的C 1-6烷基，R 7和R 10表示C 1-6烷基，芳基，C 1-6 烷氧基羰基，乙烯基，噻吩基或吡咯基，以及含有该化合物的一氧化氮测定用试剂。所述化合物可以产生在宽pH范围内没有荧光强度变化的荧光物质。

2. 发明授权

US08183060B2 Luminescent polymer and use thereof in bioassay 有权
标题翻译：发光聚合物及其在生物测定中的应用
公开(公告)号：US08183060B2
公开(公告)日：2012-05-22
申请号：US10490182
申请日：2002-09-19
申请人： Masaaki Kai
发明人： Masaaki Kai
IPC分类号： G01N33/533 , G01N33/548 , G01N21/76
CPC分类号： G01N33/54393 , C12Q1/6816 , G01N33/533 , G01N33/582 , Y10T428/2991 , Y10T428/2998 , C12Q2563/131 , C12Q2563/107
摘要： It is an object of the present invention to provide a luminescent polymer that is useful as a luminescent signal probe for labeling and detecting a target substance at high sensitivity in bioassay, and to provide the application of said luminescent polymer to bioassay.The luminescent polymer of the present invention comprises at least one biotin covalently attached to a polymer that includes monosaccharide or amino acid as a constituent monomer covalently attached to a luminescent substance. Preferably, two or more biotins are attached. Examples of the above-mentioned luminescent substance include cyanoisoindoles, luminols, and acridinium esters, and examples of the polymer include polysaccharides, polyamino acids, peptides, polypeptides, and proteins.
摘要翻译：本发明的目的是提供一种发光聚合物，其可用作用于在生物测定中以高灵敏度标记和检测靶物质的发光信号探针，并提供将所述发光聚合物应用于生物测定。本发明的发光聚合物包含至少一种共价连接到包含单糖或氨基酸作为共价连接到发光物质上的构成单体的聚合物的生物素。优选地，连接两个或更多个生物素。上述发光物质的实例包括氰基异吲哚，发光体和吖啶酯，聚合物的实例包括多糖，聚氨基酸，肽，多肽和蛋白质。

3. 发明申请

US20090291461A1 METHOD OF SELECTIVELY ASSAYING ADIPONECTIN MULTIMERS 有权
标题翻译：选择ADIPONECTIN多聚体的方法
公开(公告)号：US20090291461A1
公开(公告)日：2009-11-26
申请号：US12468440
申请日：2009-05-19
申请人： Hiroyuki EBINUMA , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi , Kazuo Hara
发明人： Hiroyuki EBINUMA , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi , Kazuo Hara
IPC分类号： G01N33/53
CPC分类号： G01N33/74 , G01N2800/042 , G01N2800/32
摘要： Kits and methods for selectively assaying a target adiponectin multimer in a biological sample. Such methods accurately evaluate the relationship between a disease and adiponectin through selective assay of adiponectin multimers and provide information that cannot be obtained through measurement of the total amount of adiponectin alone. A method for selectively assaying a target adiponectin multimer in a biological sample comprising distinguishing target adiponectin multimer from the other adiponectin multimers by using a protease and/or an antibody.
摘要翻译：用于选择性测定生物样品中目标脂联素多聚体的试剂盒和方法。这种方法通过脂联素多聚体的选择性测定来准确地评估疾病与脂联素之间的关系，并提供通过单独测量脂连蛋白的总量不能获得的信息。一种用于选择性测定生物样品中的目标脂联素多聚体的方法，包括通过使用蛋白酶和/或抗体将目标脂连蛋白多聚体与其它脂联素多聚体区分开。

4. 发明授权

US07544438B2 Electron mediator, electron mediator immobilized electrode, and biofuel cell using the electrode 有权
标题翻译：电子介体，电子介体固定电极和使用电极的生物燃料电池
公开(公告)号：US07544438B2
公开(公告)日：2009-06-09
申请号：US10807489
申请日：2004-03-23
申请人： Matsuhiko Nishizawa , Tomokazu Matsue , Jun-ichi Kosuge , Noboru Fukasaku
发明人： Matsuhiko Nishizawa , Tomokazu Matsue , Jun-ichi Kosuge , Noboru Fukasaku
IPC分类号： H01M2/14 , H01M4/86 , C12Q1/00 , C12N11/08
CPC分类号： C07C66/02 , C07C235/78 , H01M8/16 , Y02E60/527 , Y10S435/817
摘要： An object of the present invention is to provide a highly functional mediator capable of smoothly proceeding electron transfer between an electrode and a biocatalyst based on the premise that the mediator excels in safety and cost and to provide an electrode and a cell with sufficient amount of such a mediator molecule immobilized efficiently and strongly. The present invention provides a mediator mediating electron transfer between an enzyme and an electrode and containing a quinone molecule derivative, in particular, a mediator containing a VK3 derivative. In addition, the present invention provides an electrode and a biofuel cell to which those mediators are applied.
摘要翻译：本发明的目的是提供一种能够平稳地进行电极和生物催化剂之间的电子转移的高功能介体，其基于这样的前提，即介质优良的安全性和成本，并且提供具有足够量的这样的电极和电池一种有效强固地固定的介体分子。本发明提供介导酶和电极之间的电子转移并含有醌分子衍生物，特别是含有VK3衍生物的介体的介体。此外，本发明提供一种电极和生物燃料电池，其中应用这些介体。

5. 发明申请

US20080261314A1 FLUORESCENT PROBE FOR ZINC 审中-公开
标题翻译：荧光探针
公开(公告)号：US20080261314A1
公开(公告)日：2008-10-23
申请号：US12058289
申请日：2008-03-28
申请人： Tetsuo NAGANO , Kazuya Kikuchi , Tomoya Hirano , Satoko Maruyama
发明人： Tetsuo NAGANO , Kazuya Kikuchi , Tomoya Hirano , Satoko Maruyama
IPC分类号： G01N33/20 , C07D413/14 , C07D401/14
CPC分类号： C07D405/14 , C07D413/14 , C09K11/06 , C09K2211/1011 , C09K2211/1014 , C09K2211/1029 , C09K2211/1033 , C09K2211/1088 , C09K2211/188 , G01N31/22
摘要： A compound represented by the following general formula (I) or a salt thereof: wherein R1 represents hydrogen atom, an alkyl group, an alkoxy group, or hydroxy group; R2 represents a group represented by the following formula (A): wherein X1 to X4 represent hydrogen atom, an alkyl group, or 2-pyridylmethyl group, and m and n represent 0 or 1; Y represents a single bond or —CO—; R3 represents a carboxy-substituted aryl group, a carboxy-substituted heteroaryl group, benzothiazol-2-yl group, or 5-oxo-2-thioxo-4-imidazolyzinylidenmethyl group], and a fluorescent probe for zinc which comprises said compound or a salt thereof.
摘要翻译：由下列通式（I）表示的化合物或其盐：其中R 1表示氢原子，烷基，烷氧基或羟基; R 2表示由下式（A）表示的基团：其中X 1至X 4表示氢原子，烷基或 2-吡啶基甲基，m和n表示0或1; Y表示单键或-CO-; R 3表示羧基取代的芳基，羧基取代的杂芳基，苯并噻唑-2-基或5-氧代-2-硫代-4-咪唑基亚乙烯基甲基]和荧光探针对于包含所述化合物或其盐的锌。

6. 发明申请

US20080199847A1 Method for Predicting the Metabolism of Drug in Human Liver and Liver Function 审中-公开
标题翻译：预测人肝脏和肝功能中药物代谢的方法
公开(公告)号：US20080199847A1
公开(公告)日：2008-08-21
申请号：US11660592
申请日：2005-08-19
申请人： Shin-ichi Ninomiya , Yoshihiro Ohzone , Yasuhisa Adachi , Toru Horie , Yoshinori Soeno , Tae Inoue
发明人： Shin-ichi Ninomiya , Yoshihiro Ohzone , Yasuhisa Adachi , Toru Horie , Yoshinori Soeno , Tae Inoue
IPC分类号： C12Q1/00
CPC分类号： G01N33/5038 , G01N33/5088
摘要： The invention provides a method for precisely predicting the metabolism of a drug in human liver and the effect of the drug on liver function. The invention provides a method for predicting a metabolite of a test substance produced in human liver and functions of the liver, wherein the method includes administering a test substance to a nonhuman chimeric animal and a non-chimeric animal of the same species, wherein the chimeric animal intracorporeally carries a population of human-origin hepatocytes having proliferation potential and in which the hepatocyte, population substantially functions as the liver of the chimeric animal, and wherein these animals are protected from the attack by a human complement produced by the hepatocytes; and analyzing and comparing metabolites from the chimeric animal and the non-chimeric animal.
摘要翻译：本发明提供了一种精确预测人肝脏药物代谢及肝功能的作用的方法。本发明提供一种用于预测在人肝脏中产生的测试物质和肝脏功能的代谢物的方法，其中所述方法包括向非人类嵌合动物和相同物种的非嵌合动物施用测试物质，其中嵌合动物体内携带具有增殖潜力的人源肝细胞群体，其中肝细胞群体基本上起嵌合动物的肝脏的作用，并且其中这些动物被免于由肝细胞产生的人补体的攻击; 并分析和比较来自嵌合动物和非嵌合动物的代谢物。

7. 发明申请

US20080131911A1 Method for Quantitatively Determining LDL Cholesterols 审中-公开
标题翻译：定量测定低密度脂蛋白胆固醇的方法
公开(公告)号：US20080131911A1
公开(公告)日：2008-06-05
申请号：US12025369
申请日：2008-02-04
申请人： Mitsuhiro NAKAMURA , Kazuo Nakanishi , Koichi Hino , Mitsuhisa Manabe
发明人： Mitsuhiro NAKAMURA , Kazuo Nakanishi , Koichi Hino , Mitsuhisa Manabe
IPC分类号： G01N33/53 , C12Q1/60
CPC分类号： C12Q1/60
摘要： A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholesterols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.
摘要翻译：一种定量测定LDL胆固醇的方法，包括以下步骤：向血清中加入选自聚氧乙烯亚烷基苯基醚和聚氧乙烯亚烷基三苄基苯基醚的表面活性剂和胆固醇分析酶试剂，以便优先反应高密度和非常低密度的胆固醇中的胆固醇在脂蛋白中，然后确定其后反应的胆固醇的量。该方法可以消除离心和电泳等预处理的必要性，能够以高效，简单的方式进行定量测定，可以应用于各种自动分析装置。

8. 发明授权

US07329520B2 Fructosyl peptide oxidase and utilization thereof 有权
标题翻译：果糖基肽氧化酶及其利用
公开(公告)号：US07329520B2
公开(公告)日：2008-02-12
申请号：US10531305
申请日：2003-10-23
申请人： Hiroyuki Ebinuma
发明人： Hiroyuki Ebinuma
IPC分类号： C12P21/06 , C12N9/24 , C12N9/06
CPC分类号： C07K14/805 , C12N9/0022 , C12N9/2402 , C12P21/00 , C12P21/005 , C12Q1/26 , C12Q1/34 , G01N33/68 , G01N33/725 , G01N2400/00
摘要： The present invention is directed to a defructosylation enzyme originating from a plant, a method of defructosylating a fructosylated peptide or protein through use of the enzyme, and a method of measuring a fructosylated peptide or protein.
摘要翻译：本发明涉及来自植物的脱甲硅烷基化酶，通过使用酶使果糖基化肽或蛋白质去结构化的方法，以及测量果糖基化肽或蛋白质的方法。

9. 发明申请

US20080009077A1 Anti-Human Soluble Fibrin Monoclonal Antibody and Immunilogical Assay Method Using the Antibody 有权
标题翻译：抗人溶纤维蛋白单克隆抗体和使用抗体的免疫测定方法
公开(公告)号：US20080009077A1
公开(公告)日：2008-01-10
申请号：US11722998
申请日：2005-12-27
申请人： Masanao Matsuo , Hiroyuki Ebinuma , Osamu Miyazaki , Kyoko Tanaka , Akiko Suzuki
发明人： Masanao Matsuo , Hiroyuki Ebinuma , Osamu Miyazaki , Kyoko Tanaka , Akiko Suzuki
IPC分类号： G01N33/566 , C07K16/36 , C12N5/12
CPC分类号： C07K16/18 , C07K14/745 , C07K14/75 , G01N33/86 , G01N2333/75 , G01N2800/224
摘要： The present invention is directed to a monoclonal antibody against a soluble fibrin, which specifically recognizes a conformation-changed site newly occurred in a C-terminal region of an Aα-chain of the soluble fibrin formed through thrombin digestion of fibrinogen. The present invention is also directed to a hybridoma which produces the antibody, an immunological assay method employing the antibody, and a method for evaluating hypercoagulability in a test sample by measuring the soluble fibrin level in the sample with the assay method. Through employment of the monoclonal antibody of the present invention, soluble fibrin on which plasmin has not acted, which reflects exclusively initial hypercoagulability, can be specifically detected.
摘要翻译：本发明涉及针对可溶性纤维蛋白的单克隆抗体，其特异性识别通过纤维蛋白原的凝血酶消化形成的可溶性纤维蛋白的Aalpha链的C末端区域中新发生的构象改变部位。本发明还涉及产生抗体的杂交瘤，使用抗体的免疫测定方法，以及通过使用测定方法测量样品中的可溶性纤维蛋白水平来评估测试样品中的高凝状态的方法。通过使用本发明的单克隆抗体，可以特异性检测仅反映初始高凝状态的纤维蛋白溶酶未起作用的可溶性纤维蛋白。

10. 发明申请

US20070178547A1 Method of measuring glycated protein 审中-公开
标题翻译：测定糖化蛋白质的方法
公开(公告)号：US20070178547A1
公开(公告)日：2007-08-02
申请号：US10592800
申请日：2005-03-16
申请人： Yuriko Taniguchi , Tomohisa Nishio , Kazunori Saito
发明人： Yuriko Taniguchi , Tomohisa Nishio , Kazunori Saito
IPC分类号： C12Q1/37 , C12Q1/26
CPC分类号： G01N33/723 , C12Q1/26 , G01N33/6803
摘要： A method for measuring a glycated protein, a glycated peptide, or a glycated amino acid is provided. In this method, proteolytic activity of the protease is controlled to thereby realize high accuracy of the measurement. Also provided is a reagent used in such a measurement. More specifically, this invention provides a method of measuring a glycated protein, a glycated peptide, or a glycated amino acid comprising the steps of treating a sample containing the glycated protein with a protease for releasing a glycated peptide or a glycated amino acid; reacting the released glycated peptide or glycated amino acid with corresponding oxidase for generation of hydrogen peroxide; and measuring the resulting hydrogen peroxide with peroxidase and an oxidizable color developing reagent; wherein reaction solution before the reaction with the oxidase is adjusted to a pH of 1 to 5; and a reagent used in such measurement.
摘要翻译：提供了测定糖化蛋白质，糖化肽或糖化氨基酸的方法。在该方法中，控制蛋白酶的蛋白水解活性，从而实现高精度的测定。还提供了用于这种测量中的试剂。更具体地，本发明提供了测定糖化蛋白质，糖化肽或糖化氨基酸的方法，其包括用用于释放糖化肽或糖化氨基酸的蛋白酶处理含有糖基化蛋白质的样品的步骤; 使释放的糖化肽或糖化氨基酸与相应的氧化酶反应产生过氧化氢; 并用过氧化物酶和可氧化显色试剂测量所得的过氧化氢; 其中将与氧化酶反应之前的反应溶液调节至1至5的pH; 和用于这种测量的试剂。

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