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    • 6. 发明授权
    • Pepper gametoclonal variation
    • 辣椒对比变种
    • US5066830A
    • 1991-11-19
    • US142961
    • 1988-01-12
    • Robert A. MorrisonDavid A. Evans
    • Robert A. MorrisonDavid A. Evans
    • A01H4/00A01H5/08
    • A01H5/08A01H4/00
    • The present invention relates to a method of recovering variants of species of the genus Capsicum by in vitro culture which comprises:(a) culturing excised anthers on a first medium which promotes the initiation of embryos for a period of time and under conditions sufficient for initiation to occur;(b) culturing initiated anthers in a second medium which promotes embryo development, the second medium comprising a first solid layer containing an effective amount of activated charcoal and a second aqueous layer containing no charcoal for a period of time and under conditions sufficient for embryo development to occur; and(c) recovering variant plantlets from embryos so produced.
    • 本发明涉及通过体外培养来回收辣椒属种的变体的方法,其包括:(a)在第一培养基上培养切除的花药,所述第一培养基促进胚胎起始一段时间并在足以引发的条件下 发生; (b)在促进胚胎发育的第二培养基中培养引发的花药,所述第二培养基包含含有有效量的活性炭的第一固体层和不含木炭的第二含水层一段时间和足以进行胚胎发育的条件 发生; 和(c)从如此生产的胚​​胎中回收变种植物。