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1. 发明授权

US10976309B2 Methods, compositions and kits for labeling of proteins 有权
公开(公告)号：US10976309B2
公开(公告)日：2021-04-13
申请号：US16101234
申请日：2018-08-10
申请人： Biotium, Inc.
发明人： Fei Mao , Lori Michelle Roberts , Chingying Cheung
IPC分类号： G01N33/532
摘要： Provided herein are methods, compositions and kits for labeling of target proteins such as antibodies. The methods, compositions and kits are suited for labeling of such proteins that are mixed with other non-target molecules including BSA, gelatin, and other complex biological molecules.

2. 发明申请

US20160237421A9 ENZYME SUBSTRATE COMPRISING A FUNCTIONAL DYE AND ASSOCIATED TECHNOLOGY AND METHODS 有权
公开(公告)号：US20160237421A9
公开(公告)日：2016-08-18
申请号：US14051187
申请日：2013-10-10
申请人： Biotium, Inc.
发明人： Fei Mao , Hui Cen , Wai-Yee Leung
IPC分类号： C12N9/96
CPC分类号： C12N9/96 , C07D263/60 , C07D417/06 , C07D501/00 , C07K7/06 , C12Q1/37 , C12Q1/48 , C12Q1/485 , G01N21/6486 , G01N33/582
摘要： Enzyme substrates and associated technology of the present invention are provided. An enzyme substrate of the invention may comprise a biologically functional fluorescent dye and an enzyme-specific substrate moiety attached in such a way that the functionality of the functional dye is diminished. An enzymatic reaction may cleave at least a portion of the substrate moiety from the enzyme substrate to provide a more functional product dye. This product dye may be nonfluorescent or weakly fluorescent, in general, and relatively fluorescent, in a particular condition, such as when bound to a partner biological molecule or an assembly of partner biological molecules. An enzyme substrate of the present invention may thus be useful in fluorescence detection, and/or in any of a variety of useful applications, such as the detection of enzymatic activity in a cell-free system or in a living cell, the screening of drugs, or the diagnosis of disease.

3. 发明授权

US09193746B2 Luminescent metal complexes and associated technology 有权
标题翻译：发光金属配合物及相关技术
公开(公告)号：US09193746B2
公开(公告)日：2015-11-24
申请号：US11952867
申请日：2007-12-07
申请人： Fei Mao , Wai-Yee Leung , Ching-Ying Cheung , Jie Yang
发明人： Fei Mao , Wai-Yee Leung , Ching-Ying Cheung , Jie Yang
IPC分类号： G01N21/64 , C07F15/00 , C09B57/10 , G01N33/68
CPC分类号： C07F15/0053 , C09B57/10 , G01N33/6839 , G01N2458/40
摘要： Luminescent metal complexes, methods of producing and/or designing same, methods of using same, and associated technology, are disclosed herein. A luminescent metal complex may be useful for a variety of applications, such as staining, detection, and/or identification, for example, of substances, such as poly(amino acids), for example. Further by way of example, a luminescent metal complex may be useful for staining, detecting, and/or identifying poly(amino acids) that are associated with any of various environments, such as a gel or a gel matrix, such as any associated with SDS-PAGE, for example, a surface environment, such as any associated with western blot, for example, and/or the like. Compositions, solutions, and kits comprising a luminescent metal complex are also disclosed herein.
摘要翻译：本文公开了发光金属络合物，其制备和/或设计方法，使用方法及相关技术。发光金属络合物可用于各种应用，例如染色，检测和/或鉴定，例如物质，例如聚（氨基酸）。进一步作为示例，发光金属络合物可用于染色，检测和/或鉴定与各种环境中的任何环境相关的聚（氨基酸），例如凝胶或凝胶基质，例如任何与例如，SDS-PAGE，例如表面环境，例如与western印迹相关的任何例如和/或类似物。本文还公开了包含发光金属络合物的组合物，溶液和试剂盒。

4. 发明申请

US20140206099A1 METHODS, COMPOSITIONS AND KITS FOR LABELING OF PROTEINS 审中-公开
标题翻译：方法，蛋白质标记组合物和试剂盒
公开(公告)号：US20140206099A1
公开(公告)日：2014-07-24
申请号：US14030993
申请日：2013-09-18
申请人： BIOTIUM, INC.
发明人： Fei Mao , Lori M. Roberts , Chingying Cheung
IPC分类号： G01N33/536
CPC分类号： G01N33/532
摘要： Provided herein are methods, compositions and kits for labeling of target proteins such as antibodies. The methods, compositions and kits are suited for labeling of such proteins that are mixed with other non-target molecules including BSA, gelatin, and other complex biological molecules.
摘要翻译：本文提供了用于标记靶蛋白如抗体的方法，组合物和试剂盒。方法，组合物和试剂盒适用于与其他非靶分子（包括BSA，明胶和其他复杂生物分子）混合的蛋白质的标记。

5. 发明申请

US20130167309A1 METHODS OF USING DYES IN ASSOCIATION WITH NUCLEIC ACID STAINING OR DETECTION AND ASSOCIATED TECHNOLOGY 有权
标题翻译：使用染料与核酸染色或检测及相关技术相结合的方法
公开(公告)号：US20130167309A1
公开(公告)日：2013-07-04
申请号：US13541313
申请日：2012-07-03
申请人： Fei Mao , Wai-Yee Leung
发明人： Fei Mao , Wai-Yee Leung
IPC分类号： G01N1/30
CPC分类号： C09B15/00 , C09B11/28 , C09B23/04 , C12Q1/6818 , G01N1/30 , G01N21/6486 , Y10S435/81 , Y10T436/143333 , C12Q2525/301
摘要： Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. Such a dye and a salt that comprises an anion that is associated with a strong acid and a cation that is associated with a strong base may be used in such an application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids.
摘要翻译：提供了使用染料和相关技术的方法。可以在核酸凝胶染色应用和/或核酸检测应用中使用染料，例如单体染料或二聚染料。这种染料和包含与强酸相关的阴离子和与强碱相关的阳离子的盐可用于这种应用中。可以使用二聚染料，例如能够形成发夹状结构的二聚染料，以通过按需释放机制对核酸进行染色和/或检测。在存在核酸时具有低背景荧光的二聚染料和在存在核酸时与核酸存在时的高荧光可用于染色和/或检测核酸。

6. 发明申请

US20120252063A1 SUBSTITUTED ANTHRAQUINONE DYES FOR CELLULAR STAINS AND ENZYME DETECTION 有权
标题翻译：用于细胞色素和酶检测的替代蒽醌染料
公开(公告)号：US20120252063A1
公开(公告)日：2012-10-04
申请号：US13439821
申请日：2012-04-04
申请人： Fei Mao , Wai-Yee Leung , Ching-Ying Cheung , Lori Michelle Roberts
发明人： Fei Mao , Wai-Yee Leung , Ching-Ying Cheung , Lori Michelle Roberts
IPC分类号： C09B1/516 , G01N1/30 , G01N21/64
CPC分类号： C09B1/516 , A61K49/0052 , C09B1/501 , C09B1/51 , C09B1/5155 , G01N1/30 , G01N21/6428
摘要： The invention discloses new substituted anthraquinone dyes that may be useful as cellular stains. In some aspect of the invention, the nuclear stains are useful for staining the nuclei of dead or fixed cells. Another aspect of the invention relates to substituted anthraquinone dyes comprising an enzyme substrate moiety that is transformable or cleavable by an enzyme such that the transformation or cleavage of the substrate moiety causes a detectable change in the functionality or spectral properties of the dye.
摘要翻译：本发明公开了可用作细胞污渍的新的取代的蒽醌染料。在本发明的某些方面，核染料可用于染色死细胞或固定细胞的细胞核。本发明的另一方面涉及包含酶底物部分的取代的蒽醌染料，其可由酶转化或切割，使得底物部分的转化或切割导致染料的官能度或光谱性质的可检测变化。

7. 发明申请

US20100323453A1 Methods of Using Dyes in Association with Nucleic Acid Staining or Detection and Associated Technology 有权
标题翻译：使用染料与核酸染色或检测及相关技术相结合的方法
公开(公告)号：US20100323453A1
公开(公告)日：2010-12-23
申请号：US12854436
申请日：2010-08-11
申请人： Fei Mao , Wai-Yee Leung
发明人： Fei Mao , Wai-Yee Leung
IPC分类号： G01N33/52
CPC分类号： C09B15/00 , C09B11/28 , C09B23/04 , C12Q1/6818 , G01N1/30 , G01N21/6486 , Y10S435/81 , Y10T436/143333 , C12Q2525/301
摘要： Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. Such a dye and a salt that comprises an anion that is associated with a strong acid and a cation that is associated with a strong base may be used in such an application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids.
摘要翻译：提供了使用染料和相关技术的方法。可以在核酸凝胶染色应用和/或核酸检测应用中使用染料，例如单体染料或二聚染料。这种染料和包含与强酸相关的阴离子和与强碱相关的阳离子的盐可用于这种应用中。可以使用二聚染料，例如能够形成发夹状结构的二聚染料，以通过按需释放机制对核酸进行染色和/或检测。在存在核酸时具有低背景荧光的二聚染料和在存在核酸时与核酸存在时的高荧光可用于染色和/或检测核酸。

8. 发明授权

US10837881B2 Fluorescent dyes, fluorescent dye kits, and methods of preparing labeled molecules 有权
公开(公告)号：US10837881B2
公开(公告)日：2020-11-17
申请号：US15815035
申请日：2017-11-16
申请人： Biotium, Inc.
发明人： Fei Mao , Wai-Yee Leung , Ching-Ying Cheung , Hye Eun Hoover
IPC分类号： G01N33/532 , G01N33/533 , G01N1/30 , G01N33/58 , C09B11/24 , C09B57/00 , C09B23/08 , C09B57/02 , C09B23/06 , G01N33/53 , C07K1/13 , C07K17/06 , G01N33/68
CPC分类号： G01N1/30 , C09B11/24 , C09B23/06 , C09B23/083 , C09B57/001 , C09B57/02 , G01N33/53 , G01N33/532 , G01N33/533 , G01N33/582 , C07K1/13 , C07K17/06 , G01N33/6854
摘要： The present invention provides methods, compositions, and kits useful in preparing labeled molecules, which are useful in the detection of binding partners.

9. 发明申请

US20150025222A1 Substituted Anthraquinone Dyes for Cellular Stains and Enzyme Detection 有权
标题翻译：取代的蒽醌染料用于细胞污染和酶检测
公开(公告)号：US20150025222A1
公开(公告)日：2015-01-22
申请号：US14248886
申请日：2014-04-09
申请人： Biotium, Inc.
发明人： Fei MAO , Wai-Yee LEUNG , Ching-Ying CHEUNG , Lori Michelle ROBERTS
IPC分类号： C09B1/516 , C09B1/51 , A61K49/00
CPC分类号： C09B1/516 , A61K49/0052 , C09B1/501 , C09B1/51 , C09B1/5155 , G01N1/30 , G01N21/6428
摘要： The invention discloses new substituted anthraquinone dyes that may be useful as cellular stains. In some aspect of the invention, the nuclear stains are useful for staining the nuclei of dead or fixed cells. Another aspect of the invention relates to substituted anthraquinone dyes comprising an enzyme substrate moiety that is transformable or cleavable by an enzyme such that the transformation or cleavage of the substrate moiety causes a detectable change in the functionality or spectral properties of the dye.
摘要翻译：本发明公开了可用作细胞污渍的新的取代的蒽醌染料。在本发明的某些方面，核染料可用于染色死细胞或固定细胞的细胞核。本发明的另一方面涉及包含酶底物部分的取代的蒽醌染料，其可由酶转化或切割，使得底物部分的转化或切割导致染料的官能度或光谱性质的可检测变化。

10. 发明授权

US08790892B2 Substituted anthraquinone dyes for cellular stains and enzyme detection 有权
标题翻译：取代的蒽醌染料用于细胞染色和酶检测
公开(公告)号：US08790892B2
公开(公告)日：2014-07-29
申请号：US13439821
申请日：2012-04-04
申请人： Fei Mao , Wai-Yee Leung , Ching-Ying Cheung , Lori Michelle Roberts
发明人： Fei Mao , Wai-Yee Leung , Ching-Ying Cheung , Lori Michelle Roberts
IPC分类号： G01N1/30
CPC分类号： C09B1/516 , A61K49/0052 , C09B1/501 , C09B1/51 , C09B1/5155 , G01N1/30 , G01N21/6428
摘要： The invention discloses new substituted anthraquinone dyes that may be useful as cellular stains. In some aspect of the invention, the nuclear stains are useful for staining the nuclei of dead or fixed cells. Another aspect of the invention relates to substituted anthraquinone dyes comprising an enzyme substrate moiety that is transformable or cleavable by an enzyme such that the transformation or cleavage of the substrate moiety causes a detectable change in the functionality or spectral properties of the dye.
摘要翻译：本发明公开了可用作细胞污渍的新的取代的蒽醌染料。在本发明的某些方面，核染料可用于染色死细胞或固定细胞的细胞核。本发明的另一方面涉及包含酶底物部分的取代的蒽醌染料，其可由酶转化或切割，使得底物部分的转化或切割导致染料的官能度或光谱性质的可检测变化。

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